Entity Extraction Examples

(0)

CheY - dependent methylation of the asparagine receptor, McpB, during chemotaxis in Bacillus subtilis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9980	CheY - dependent methylation of the asparagine receptor, ProteinMcpB , during chemotaxis in Bacillus subtilis.
0.9196	ProteinCheY - dependent methylation of the asparagine receptor, McpB, during chemotaxis in Bacillus subtilis.
0.7265	CheY - dependent Methylationmethylation of the asparagine receptor, McpB, during chemotaxis in Bacillus subtilis.

(1)

For the Gram - positive organism Bacillus subtilis, chemotaxis to the attractant asparagine is mediated by the chemoreceptor McpB.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	For the Gram - positive organism Bacillus subtilis, chemotaxis to the attractant asparagine is mediated by the chemoreceptor ProteinMcpB .

(2)

In this study, we show that rapid net demethylation of B. subtilis McpB results in the immediate production of methanol, presumably due to the action of CheB.

Precision: 66.67

Recall: 66.67

F1: 66.67

Score	Text
0.9935	In this study, we show that rapid net demethylation of B. subtilis ProteinMcpB results in the immediate production of methanol, presumably due to the action of CheB.
0.9912	In this study, we show that rapid net demethylation of B. subtilis McpB results in the immediate production of methanol, presumably due to the action of ProteinCheB .
0.6187	In this study, we show that rapid net Methylationdemethylation of B. subtilis McpB results in the immediate production of methanol, presumably due to the action of CheB.
	In this study, we show that rapid net Demethylationdemethylation of B. subtilis McpB results in the immediate production of methanol, presumably due to the action of CheB.

(3)

We also show that net demethylation of McpB occurs upon both addition and removal of asparagine.

Precision: 50.00

Recall: 50.00

F1: 50.00

Score	Text
0.9993	We also show that net demethylation of ProteinMcpB occurs upon both addition and removal of asparagine.
0.4519	We also show that net Methylationdemethylation of McpB occurs upon both addition and removal of asparagine.
	We also show that net Demethylationdemethylation of McpB occurs upon both addition and removal of asparagine.

(4)

After each demethylation event, McpB is remethylated to nearly prestimulus levels.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9983	After each demethylation event, ProteinMcpB is remethylated to nearly prestimulus levels.
0.8137	After each demethylation event, McpB is Methylationremethylated to nearly prestimulus levels.

(5)

Both remethylation events are attributable to CheR using S - adenosylmethionine as a substrate.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9985	Both remethylation events are attributable to ProteinCheR using S - adenosylmethionine as a substrate.
0.8293	Both Methylationremethylation events are attributable to CheR using S - adenosylmethionine as a substrate.
	Both Catalysisremethylation events are attributable to CheR using S - adenosylmethionine as a substrate.

(6)

Therefore, no methyl transfer to an intermediate carrier need be postulated to occur during chemotaxis in B. subtilis as was previously suggested.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(7)

Furthermore, we show that the remethylation of asparagine - bound McpB requires the response regulator, CheY - P, suggesting that CheY - P acts in a feedback mechanism to facilitate adaptation to positive stimuli during chemotaxis in B. subtilis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9983	Furthermore, we show that the remethylation of asparagine - bound McpB requires the response regulator, CheY - P, suggesting that ProteinCheY - P acts in a feedback mechanism to facilitate adaptation to positive stimuli during chemotaxis in B. subtilis.
0.9972	Furthermore, we show that the remethylation of asparagine - bound McpB requires the response regulator, ProteinCheY - P , suggesting that CheY - P acts in a feedback mechanism to facilitate adaptation to positive stimuli during chemotaxis in B. subtilis.
0.9880	Furthermore, we show that the remethylation of asparagine - bound ProteinMcpB requires the response regulator, CheY - P, suggesting that CheY - P acts in a feedback mechanism to facilitate adaptation to positive stimuli during chemotaxis in B. subtilis.
0.8810	Furthermore, we show that the Methylationremethylation of asparagine - bound McpB requires the response regulator, CheY - P, suggesting that CheY - P acts in a feedback mechanism to facilitate adaptation to positive stimuli during chemotaxis in B. subtilis.

(8)

This hypothesis is supported by two observations : a cheRBCD mutant is capable of transient excitation and subsequent oscillations that bring the flagellar rotational bias below the prestimulus value in the tethered cell assay, and the cheRBCD mutant is capable of swarming in a Tryptone swarm plate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	This hypothesis is supported by two observations : a cheRBCD mutant is capable of transient excitation and subsequent oscillations that bring the flagellar rotational bias below the prestimulus value in the tethered cell assay, and the ProteincheRBCD mutant is capable of swarming in a Tryptone swarm plate.
0.9998	This hypothesis is supported by two observations : a ProteincheRBCD mutant is capable of transient excitation and subsequent oscillations that bring the flagellar rotational bias below the prestimulus value in the tethered cell assay, and the cheRBCD mutant is capable of swarming in a Tryptone swarm plate.

(9)

Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9647	Transglycosylation reactions of Bacillus stearothermophilus Proteinmaltogenic amylase with acarbose and various acceptors.

(10)

It was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide ( PTS ) that was transferred to C - 6 of the glucose to give an alpha - ( 1 - - > 6 ) glycosidic linkage and the formation of isoacarbose.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9884	It was observed that Bacillus stearothermophilus Proteinmaltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide ( PTS ) that was transferred to C - 6 of the glucose to give an alpha - ( 1 - - > 6 ) glycosidic linkage and the formation of isoacarbose.
0.5754	It was observed that Bacillus stearothermophilus maltogenic Proteinamylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide ( PTS ) that was transferred to C - 6 of the glucose to give an alpha - ( 1 - - > 6 ) glycosidic linkage and the formation of isoacarbose.

(11)

The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha - ( 1 - - > 6 ) to D - glucose, D - mannose, D - galactose, and methyl alpha - D - glucopyranoside.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(12)

With D - fructopyranose and D - xylopyranose, PTS was linked alpha - ( 1 - - > 5 ) and alpha - ( 1 - - > 4 ), respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(13)

PTS was primarily transferred to C - 6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(14)

Lesser amounts of alpha - ( 1 - - > 3 ) and / or alpha - ( 1 - - > 4 ) transfer products were also observed for these carbohydrate acceptors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(15)

The major transfer product to sucrose gave PTS linked alpha - ( 1 - - > 4 ) to the glucose residue. alpha, alpha - Trehalose gave two major products with PTS linked alpha - ( 1 - - > 6 ) and alpha - ( 1 - - > 4 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(16)

Maltitol gave two major products with PTS linked alpha - ( 1 - - > 6 ) and alpha - ( 1 - - > 4 ) to the glucopyranose residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(17)

Raffinose gave two major products with PTS linked alpha - ( 1 - - > 6 ) and alpha - ( 1 - - > 4 ) to the D - galactopyranose residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(18)

Maltotriose gave two major products with PTS linked alpha - ( 1 - - > 6 ) and alpha - ( 1 - - > 4 ) to the nonreducing end glucopyranose residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(19)

Xylitol gave PTS linked alpha - ( 1 - - > 5 ) as the major product and D - glucitol gave PTS linked alpha - ( 1 - - > 6 ) as the only product.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(20)

The structures of the transfer products were determined using thin - layer chromatography, high - performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(21)

The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D - glucitol.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(22)

A Plasmodium chabaudi chabaudi high molecular mass glycoprotein translocated to the host cell membrane by a non - classical secretory pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(23)

We have purified and characterized a novel high molecular mass glycoprotein of P. chabaudi chabaudi ( Pc550gp ) that is transported to the erythrocyte membrane during the intraerythrocytic cycle.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9562	We have purified and characterized a novel high molecular mass glycoprotein of P. chabaudi chabaudi Protein( Pc550gp ) that is transported to the erythrocyte membrane during the intraerythrocytic cycle.

(24)

Immuno fluorescence assays with polyclonal monospecific antibodies against Pc550gp show that the protein to be localized in the periphery of young trophozoite stages i. e., on the plasma membrane or parasitophorous vacuole membrane.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Immuno fluorescence assays with polyclonal monospecific antibodies against ProteinPc550gp show that the protein to be localized in the periphery of young trophozoite stages i. e., on the plasma membrane or parasitophorous vacuole membrane.

(25)

However, in late trophozoites and schizonts the antigen is distributed in both parasite and host cell membranes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(26)

These results were confirmed by immunoblotting of isolated parasites and infected host cell membranes at different stages of parasite development.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(27)

Moreover, alkali extraction of purified infected erythrocyte membranes at mature stages of parasite development does not solubilize Pc550gp, suggesting that it is an integral membrane protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Moreover, alkali extraction of purified infected erythrocyte membranes at mature stages of parasite development does not solubilize ProteinPc550gp , suggesting that it is an integral membrane protein.

(28)

In addition proteinase K digestion of intact infected host cells induced the disappearance of Pc550gp.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	In addition proteinase K digestion of intact infected host cells induced the disappearance of ProteinPc550gp .

(29)

Further indicating its transmembrane nature and that it presents extracellular domains susceptible to proteolysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(30)

Brefeldin A or low temperature ( 15 degrees C ) treatment did not affect the translocation of Pc550gp from the parasite compartments to the erythrocyte membrane, indicating that the secretion of Pc550gp does not follow the classical transport pathway described in most eukaryotic cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Brefeldin A or low temperature ( 15 degrees C ) treatment did not affect the translocation of ProteinPc550gp from the parasite compartments to the erythrocyte membrane, indicating that the secretion of Pc550gp does not follow the classical transport pathway described in most eukaryotic cells.
0.9996	Brefeldin A or low temperature ( 15 degrees C ) treatment did not affect the translocation of Pc550gp from the parasite compartments to the erythrocyte membrane, indicating that the secretion of ProteinPc550gp does not follow the classical transport pathway described in most eukaryotic cells.

(31)

Novel roles for classical factors at the interface between translation termination and initiation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(32)

The pathway of bacterial ribosome recycling following translation termination has remained obscure.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(33)

Here, we elucidate two essential steps and describe the roles played by the three translation factors EF - G, RRF, and IF3.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	Here, we elucidate two essential steps and describe the roles played by the three translation factors EF - G, RRF, and ProteinIF3 .
0.9982	Here, we elucidate two essential steps and describe the roles played by the three translation factors EF - G, ProteinRRF , and IF3.
0.9976	Here, we elucidate two essential steps and describe the roles played by the three translation factors ProteinEF - G , RRF, and IF3.

(34)

Release factor RF3 is known to catalyze the dissociation of RF1 or RF2 from ribosomes after polypeptide release.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9985	Release factor RF3 is known to catalyze the dissociation of RF1 or ProteinRF2 from ribosomes after polypeptide release.
0.9982	Release factor RF3 is known to catalyze the dissociation of ProteinRF1 or RF2 from ribosomes after polypeptide release.
0.9927	Release factor ProteinRF3 is known to catalyze the dissociation of RF1 or RF2 from ribosomes after polypeptide release.

(35)

We show that the next step is dissociation of 50S subunits from the 70S posttermination complex and that it is catalyzed by RRF and EF - G and requires GTP hydrolysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9982	We show that the next step is dissociation of 50S subunits from the 70S posttermination complex and that it is catalyzed by RRF and ProteinEF - G and requires GTP hydrolysis.
0.9965	We show that the next step is dissociation of 50S subunits from the 70S posttermination complex and that it is catalyzed by ProteinRRF and EF - G and requires GTP hydrolysis.

(36)

Removal of deacylated tRNA from the resulting 30S : mRNA : tRNA posttermination complex is then necessary to permit rapid 30S subunit recycling.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(37)

We show that this step requires initiation factor IF3, whose role was previously thought to be restricted to promoting specific 30S initiation complex formation from free 30S subunits.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	We show that this step requires initiation factor ProteinIF3 , whose role was previously thought to be restricted to promoting specific 30S initiation complex formation from free 30S subunits.

(38)

Effect of alternative glycosylation on insulin receptor processing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9796	Effect of alternative Glycosylationglycosylation on insulin receptor processing.
0.9509	Effect of alternative glycosylation on Proteininsulin receptor processing.

(39)

The mature insulin receptor is a cell surface heterotetrameric glycoprotein composed of two alpha - and two beta - subunits.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9803	The mature Proteininsulin receptor is a cell surface heterotetrameric glycoprotein composed of two alpha - and two beta - subunits.

(40)

In 3T3 - L1 adipocytes as in other cell types, the receptor is synthesized as a single polypeptide consisting of uncleaved alpha - and beta - subunits, migrating as a 190 - kDa glycoprotein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(41)

To examine the importance of N - linked glycosylation on insulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9699	To examine the importance of N - linked glycosylation on Proteininsulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.
0.9253	To examine the importance of GlycosylationN - linked glycosylation on insulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.
0.9429	To examine the importance of N - linked Glycosylationglycosylation on insulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.
0.7012	To examine the importance of GlycosylationN - linked glycosylation on insulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.

(42)

Western blot analysis shows that glucose deprivation led to a time - dependent accumulation of an alternative proreceptor of 170 kDa in a subcellular fraction consistent with endoplasmic reticulum localization.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(43)

Co - precipitation assays provide evidence that the alternative proreceptor bound GRP78, an endoplasmic reticulum molecular chaperone.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9978	Co - precipitation assays provide evidence that the alternative proreceptor bound ProteinGRP78 , an endoplasmic reticulum molecular chaperone.

(44)

N - Glycosidase F treatment shows that the alternative proreceptor contained N - linked oligosaccharides.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9765	N - Glycosidase F treatment shows that the alternative proreceptor contained N - linked Entityoligosaccharides .
0.9545	N - Glycosidase F treatment shows that the alternative proreceptor contained GlycosylationN - linked oligosaccharides.
0.9442	ProteinN - Glycosidase F treatment shows that the alternative proreceptor contained N - linked oligosaccharides.

(45)

Yet, endoglycosidase H insensitivity indicates an aberrant oligosaccharide structure.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9492	Yet, Proteinendoglycosidase H insensitivity indicates an aberrant oligosaccharide structure.

(46)

Using pulse - chase methodology, we show that the synthetic rate was similar between the normal and alternative proreceptor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(47)

However, the normal proreceptor was processed into alpha - and beta - subunits ( t ( ( 1 ) / ( 2 ) ) = 1. 3 + / - 0. 6 h ), while the alternative proreceptor was degraded ( t ( ( 1 ) / ( 2 ) ) = 5. 1 + / - 0. 6 h ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(48)

Upon refeeding cells that were initially deprived of glucose, the alternative proreceptor was processed to a higher molecular weight form and gained sensitivity to endoglycosidase H.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9669	Upon refeeding cells that were initially deprived of glucose, the alternative proreceptor was processed to a higher molecular weight form and gained sensitivity to Proteinendoglycosidase H .

(49)

This " intermediate " form of the proreceptor was also degraded, although a small fraction escaped degradation, resulting in cleavage to the alpha - and beta - subunits.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(50)

These data provide evidence for the first time that glucose deprivation leads to the accumulation of an alternative proreceptor, which can be post - translationally glycosylated with the readdition of glucose inducing both accelerated degradation and maturation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(51)

Meat, metabolic genotypes and risk for colorectal cancer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(52)

Persuasive data exist as to the importance of environmental factors in the pathogenesis of sporadic colorectal cancer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(53)

One possibility is that the effect of environmental factors varies between individuals, perhaps on the basis of inherited variation ( polymorphism ) in genes which influence the activation or inactivation of dietary carcinogens.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(54)

Thus far, the focus has been on acetylator genes ( NAT1, NAT2 ) and the activation of heterocyclic amines, carcinogens generated by cooking meat for prolonged periods at high temperature.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	Thus far, the focus has been on acetylator genes ( NAT1, ProteinNAT2 ) and the activation of heterocyclic amines, carcinogens generated by cooking meat for prolonged periods at high temperature.
0.9990	Thus far, the focus has been on acetylator genes Protein( NAT1 , NAT2 ) and the activation of heterocyclic amines, carcinogens generated by cooking meat for prolonged periods at high temperature.

(55)

Three case - control studies and one prospective study have shown a consistent trend towards higher risks for cancer with higher intakes of meat in rapid acetylators for NAT1, NAT2 or both genotypes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Three case - control studies and one prospective study have shown a consistent trend towards higher risks for cancer with higher intakes of meat in rapid acetylators for NAT1, ProteinNAT2 or both genotypes.
0.9998	Three case - control studies and one prospective study have shown a consistent trend towards higher risks for cancer with higher intakes of meat in rapid acetylators for ProteinNAT1 , NAT2 or both genotypes.

(56)

Other links between meat, cooking methods, metabolic genotypes and risk for cancer might include enhanced activation of polycyclic aromatic hydrocarbons and N - nitroso compounds by variant genotypes of CYP1A1 and CYP2E1, respectively, and modulation by meat of the protective effect of the E4 allele of apolipoprotein E on risk for cancer of the proximal colon.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Other links between meat, cooking methods, metabolic genotypes and risk for cancer might include enhanced activation of polycyclic aromatic hydrocarbons and N - nitroso compounds by variant genotypes of CYP1A1 and ProteinCYP2E1 , respectively, and modulation by meat of the protective effect of the E4 allele of apolipoprotein E on risk for cancer of the proximal colon.
0.9995	Other links between meat, cooking methods, metabolic genotypes and risk for cancer might include enhanced activation of polycyclic aromatic hydrocarbons and N - nitroso compounds by variant genotypes of ProteinCYP1A1 and CYP2E1, respectively, and modulation by meat of the protective effect of the E4 allele of apolipoprotein E on risk for cancer of the proximal colon.
0.9990	Other links between meat, cooking methods, metabolic genotypes and risk for cancer might include enhanced activation of polycyclic aromatic hydrocarbons and N - nitroso compounds by variant genotypes of CYP1A1 and CYP2E1, respectively, and modulation by meat of the protective effect of the ProteinE4 allele of apolipoprotein E on risk for cancer of the proximal colon.
0.9899	Other links between meat, cooking methods, metabolic genotypes and risk for cancer might include enhanced activation of polycyclic aromatic hydrocarbons and N - nitroso compounds by variant genotypes of CYP1A1 and CYP2E1, respectively, and modulation by meat of the protective effect of the E4 allele of Proteinapolipoprotein E on risk for cancer of the proximal colon.

(57)

Differential expression of human lysyl hydroxylase genes, lysine hydroxylation, and cross - linking of type I collagen during osteoblastic differentiation in vitro.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.5464	Differential expression of human Proteinlysyl hydroxylase genes, lysine hydroxylation, and cross - linking of type I collagen during osteoblastic differentiation in vitro.

(58)

The pattern of lysyl hydroxylation in the nontriple helical domains of collagen is critical in determining the cross - linking pathways that are tissue specific.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(59)

We hypothesized that the tissue specificity of type I collagen cross - linking is, in part, due to the differential expression of lysyl hydroxylase genes ( Procollagen - lysine, 2 - oxyglutarate, 5 - dioxygenase 1, 2, and 3 [ PLOD1, PLOD2, and PLOD3 ] ).

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9991	We hypothesized that the tissue specificity of type I collagen cross - linking is, in part, due to the differential expression of lysyl hydroxylase genes ( Procollagen - lysine, 2 - oxyglutarate, 5 - dioxygenase 1, 2, and 3 [ PLOD1, ProteinPLOD2 , and PLOD3 ] ).
0.9977	We hypothesized that the tissue specificity of type I collagen cross - linking is, in part, due to the differential expression of lysyl hydroxylase genes ( Procollagen - lysine, 2 - oxyglutarate, 5 - dioxygenase 1, Protein2 , and 3 [ PLOD1, PLOD2, and PLOD3 ] ).
0.9963	We hypothesized that the tissue specificity of type I collagen cross - linking is, in part, due to the differential expression of lysyl hydroxylase genes ( Procollagen - lysine, 2 - oxyglutarate, 5 - dioxygenase 1, 2, and Protein3 [ PLOD1, PLOD2, and PLOD3 ] ).
0.9954	We hypothesized that the tissue specificity of type I collagen cross - linking is, in part, due to the differential expression of lysyl hydroxylase genes ( Procollagen - lysine, 2 - oxyglutarate, 5 - dioxygenase 1, 2, and 3 [ PLOD1, PLOD2, and ProteinPLOD3 ] ) .
0.9297	We hypothesized that the tissue specificity of type I collagen cross - linking is, in part, due to the differential expression of lysyl hydroxylase genes ( Procollagen - lysine, 2 - oxyglutarate, 5 - dioxygenase 1, 2, and 3 Protein[ PLOD1 , PLOD2, and PLOD3 ] ).
0.7111	We hypothesized that the tissue specificity of type I collagen cross - linking is, in part, due to the differential expression of lysyl hydroxylase genes Protein( Procollagen - lysine, 2 - oxyglutarate, 5 - dioxygenase 1 , 2, and 3 [ PLOD1, PLOD2, and PLOD3 ] ).
0.9363	We hypothesized that the tissue specificity of type I collagen cross - linking is, in part, due to the differential expression of lysyl hydroxylase genes ( Procollagen - lysine, 2 - oxyglutarate, 5 - dioxygenase 1, 2, and Protein3 [ PLOD1 , PLOD2, and PLOD3 ] ).
0.8143	We hypothesized that the tissue specificity of type I collagen cross - linking is, in part, due to the differential expression of lysyl hydroxylase genes ( Procollagen - lysine, 2 - oxyglutarate Protein, 5 - dioxygenase 1 , 2, and 3 [ PLOD1, PLOD2, and PLOD3 ] ).
0.7823	We hypothesized that the tissue specificity of type I collagen cross - linking is, in part, due to the differential expression of lysyl hydroxylase genes ( Procollagen - lysine Protein, 2 - oxyglutarate, 5 - dioxygenase 1 , 2, and 3 [ PLOD1, PLOD2, and PLOD3 ] ).

(60)

In this study, we have examined the expression patterns of these three genes during the course of in vitro differentiation of human osteoprogenitor cells ( bone marrow stromal cells [ BMSCs ] ) and normal skin fibroblasts ( NSFs ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(61)

In addition, using the medium and cell layer / matrix fractions in these cultures, lysine hydroxylation of type I collagen alpha chains and collagen cross - linking chemistries have been characterized.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.5110	In addition, using the medium and cell layer / matrix fractions in these cultures, lysine Hydroxylationhydroxylation of type I collagen alpha chains and collagen cross - linking chemistries have been characterized.

(62)

High levels of PLOD1 and PLOD3 genes were expressed in both BMSCs and NSFs, and the expression levels did not change in the course of differentiation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	High levels of ProteinPLOD1 and PLOD3 genes were expressed in both BMSCs and NSFs, and the expression levels did not change in the course of differentiation.
0.9991	High levels of PLOD1 and ProteinPLOD3 genes were expressed in both BMSCs and NSFs, and the expression levels did not change in the course of differentiation.

(63)

In contrast to the PLOD1 and PLOD3 genes, both cell types showed low PLOD2 gene expression in undifferentiated and early differentiated conditions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	In contrast to the ProteinPLOD1 and PLOD3 genes, both cell types showed low PLOD2 gene expression in undifferentiated and early differentiated conditions.
0.9995	In contrast to the PLOD1 and PLOD3 genes, both cell types showed low ProteinPLOD2 gene expression in undifferentiated and early differentiated conditions.
0.9994	In contrast to the PLOD1 and ProteinPLOD3 genes, both cell types showed low PLOD2 gene expression in undifferentiated and early differentiated conditions.

(64)

However, fully differentiated BMSCs, but not NSFs, exhibited a significantly elevated level ( 6 - fold increase ) of PLOD2 mRNA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	However, fully differentiated BMSCs, but not NSFs, exhibited a significantly elevated level ( 6 - fold increase ) of ProteinPLOD2 mRNA.

(65)

This increase coincided with the onset of matrix mineralization and with the increase in lysyl hydroxylation in the nontriple helical domains of alpha chains of type I collagen molecule.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(66)

Furthermore, the collagen cross - links that are derived from the nontriple helical hydroxylysine - aldehyde were found only in fully differentiated BMSC cultures.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(67)

The data suggests that PLOD2 expression is associated with lysine hydroxylation in the nontriple helical domains of collagen and, thus, could be partially responsible for the tissue - specific collagen cross - linking pattern.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	The data suggests that ProteinPLOD2 expression is associated with lysine hydroxylation in the nontriple helical domains of collagen and, thus, could be partially responsible for the tissue - specific collagen cross - linking pattern.

(68)

Phenylalanine residues in the active site of tyrosine hydroxylase : mutagenesis of Phe300 and Phe309 to alanine and metal ion - catalyzed hydroxylation of Phe300.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9778	Phenylalanine residues in the active site of Proteintyrosine hydroxylase : mutagenesis of Phe300 and Phe309 to alanine and metal ion - catalyzed hydroxylation of Phe300.

(69)

Residues Phe300 and Phe309 of tyrosine hydroxylase are located in the active site in the recently described three - dimensional structure of the enzyme, where they have been proposed to play roles in substrate binding.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9740	Residues Phe300 and Phe309 of Proteintyrosine hydroxylase are located in the active site in the recently described three - dimensional structure of the enzyme, where they have been proposed to play roles in substrate binding.

(70)

Also based on the structure, Phe300 has been reported to be hydroxylated due to a naturally occurring posttranslational modification [ Goodwill, K.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(71)

E., Sabatier, C., and Stevens, R.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(72)

C. ( 1998 ) Biochemistry 37, 13437 - 13445 ].

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(73)

Mutants of tyrosine hydroxylase with alanine substituted for Phe300 or Phe309 have now been purified and characterized.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9693	Mutants of Proteintyrosine hydroxylase with alanine substituted for Phe300 or Phe309 have now been purified and characterized.

(74)

The F309A protein possesses 40 % less activity than wild - type tyrosine hydroxylase in the production of DOPA, but full activity in the production of dihydropterin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9572	The F309A protein possesses 40 % less activity than wild - type Proteintyrosine hydroxylase in the production of DOPA, but full activity in the production of dihydropterin.

(75)

The F300A protein shows a 2. 5 - fold decrease in activity in the production of both DOPA and dihydropterin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(76)

The K ( 6 - MPH4 ) value for F300A tyrosine hydroxylase is twice the wild - type value.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.8885	The K ( 6 - MPH4 ) value for F300A Proteintyrosine hydroxylase is twice the wild - type value.
0.8410	The K ( 6 - MPH4 ) value for ProteinF300A tyrosine hydroxylase is twice the wild - type value.

(77)

These results are consistent with Phe309 having a role in maintaining the integrity of the active site, while Phe300 contributes less than 1 kcal / mol to binding tetrahydropterin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(78)

Characterization of Phe300 by MALDI - TOF mass spectrometry and amino acid sequencing showed that hydroxylation only occurs in the isolated catalytic domain after incubation with a large excess of 7, 8 - dihydropterin, DTT, and Fe ( 2 + ).

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.7185	Characterization of Phe300 by MALDI - TOF mass spectrometry and amino acid sequencing showed that Hydroxylationhydroxylation only occurs in the isolated catalytic domain after incubation with a large excess of 7, 8 - dihydropterin, DTT, and Fe ( 2 + ).

(79)

The modification is not observed in the untreated catalytic domain or in the full - length protein, even in the presence of excess iron.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(80)

These results establish that hydroxylation of Phe300 is an artifact of the crystallography conditions and is not relevant to catalysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(81)

Development of an enzyme - linked immunosorbent assay, using a monoclonal antibody against alpha2 - macroglobulin, for the diagnosis of systemic lupus erythematosus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9967	Development of an enzyme - linked immunosorbent assay, using a monoclonal antibody against Proteinalpha2 - macroglobulin , for the diagnosis of systemic lupus erythematosus.

(82)

OBJECTIVES : To develop an enzyme - linked immunosorbent assay ( ELISA ) using a monoclonal antibody ( mab ) directed against abnormally glycosylated serum alpha2 - macroglobulin ( alpha2 - M ) from patients with systemic lupus erythematosus ( SLE ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9908	OBJECTIVES : To develop an enzyme - linked immunosorbent assay ( ELISA ) using a monoclonal antibody ( mab ) directed against abnormally Glycosylationglycosylated serum alpha2 - macroglobulin ( alpha2 - M ) from patients with systemic lupus erythematosus ( SLE ).
0.9501	OBJECTIVES : To develop an enzyme - linked immunosorbent assay ( ELISA ) using a monoclonal antibody ( mab ) directed against abnormally glycosylated serum alpha2 - macroglobulin Protein( alpha2 - M ) from patients with systemic lupus erythematosus ( SLE ).
0.8773	OBJECTIVES : To develop an enzyme - linked immunosorbent assay ( ELISA ) using a monoclonal antibody ( mab ) directed against abnormally glycosylated serum Proteinalpha2 - macroglobulin ( alpha2 - M ) from patients with systemic lupus erythematosus ( SLE ).

(83)

DESIGN AND METHODS : Serum alpha2 - M purified by HPLC from patients with SLE was injected in a Balb / c, CB6 F1 female mouse and hybrid cell lines were screened using alpha2 - M Glu - C fragments derived from SLE and normal donors ( NHS ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9948	DESIGN AND METHODS : Serum alpha2 - M purified by HPLC from patients with SLE was injected in a Balb / c, CB6 F1 female mouse and hybrid cell lines were screened using Proteinalpha2 - M Glu - C fragments derived from SLE and normal donors ( NHS ).
0.9917	DESIGN AND METHODS : Serum Proteinalpha2 - M purified by HPLC from patients with SLE was injected in a Balb / c, CB6 F1 female mouse and hybrid cell lines were screened using alpha2 - M Glu - C fragments derived from SLE and normal donors ( NHS ).

(84)

A mab was selected and used to develop an ELISA by which sera from NHS ( n = 14 ), SLE ( n = 34 ), rheumatoid arthritis ( n = 15 ), Sjogren's syndrome ( n = 11 ), mixed connective tissue diseases ( n = 12 ), and liver diseases ( n = 11 ) were analyzed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(85)

RESULTS : The affinity of the mab for alpha2 - M from SLE, but not from the other diseases, was higher compared to NHS, as demonstrated by immunoblotting and ELISA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9977	RESULTS : The affinity of the mab for Proteinalpha2 - M from SLE, but not from the other diseases, was higher compared to NHS, as demonstrated by immunoblotting and ELISA.

(86)

CONCLUSIONS : The ELISA was capable of recognizing changes of glycosylation of alpha2 - M in SLE and may be useful for its differential diagnosis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9920	CONCLUSIONS : The ELISA was capable of recognizing changes of Glycosylationglycosylation of alpha2 - M in SLE and may be useful for its differential diagnosis.
0.9905	CONCLUSIONS : The ELISA was capable of recognizing changes of glycosylation of Proteinalpha2 - M in SLE and may be useful for its differential diagnosis.

(87)

Efficient adaptational demethylation of chemoreceptors requires the same enzyme - docking site as efficient methylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(88)

The mechanistic basis of sensory adaptation and gradient sensing in bacterial chemotaxis is reversible covalent modification of transmembrane chemoreceptors, methylation, and demethylation at specific glutamyl residues in their cytoplasmic domains.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(89)

These reactions are catalyzed by a dedicated methyltransferase CheR and a dedicated methylesterase CheB.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	These reactions are catalyzed by a dedicated methyltransferase ProteinCheR and a dedicated methylesterase CheB.
0.9987	These reactions are catalyzed by a dedicated methyltransferase CheR and a dedicated methylesterase ProteinCheB .

(90)

The esterase is also a deamidase that creates certain methyl - accepting glutamyls by hydrolysis of glutamine side chains.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(91)

We investigated the action of CheB and its activated form, phospho - CheB, on a truncated form of the aspartate receptor of Escherichia coli that was missing the last 5 aa of the intact receptor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	We investigated the action of ProteinCheB and its activated form, phospho - CheB, on a truncated form of the aspartate receptor of Escherichia coli that was missing the last 5 aa of the intact receptor.
0.9901	We investigated the action of CheB and its activated form, Proteinphospho - CheB , on a truncated form of the aspartate receptor of Escherichia coli that was missing the last 5 aa of the intact receptor.

(92)

The deleted pentapeptide is conserved in several chemoreceptors in enteric and related bacteria.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(93)

The truncated receptor was much less efficiently demethylated and deamidated than intact receptor, but essentially was unperturbed for kinase activation or transmembrane signaling.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(94)

CheB bound specifically to an affinity column carrying the isolated pentapeptide, implying that in the intact receptor the pentapeptide serves as a docking site for the methylesterase / deamidase and that the truncated receptor was inefficiently modified because the enzyme could not dock.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9997	ProteinCheB bound specifically to an affinity column carrying the isolated pentapeptide, implying that in the intact receptor the pentapeptide serves as a docking site for the methylesterase / deamidase and that the truncated receptor was inefficiently modified because the enzyme could not dock.
0.7522	CheB bound specifically to an affinity column carrying the isolated pentapeptide, implying that in the intact receptor the pentapeptide serves as a docking site for the Proteinmethylesterase / deamidase and that the truncated receptor was inefficiently modified because the enzyme could not dock.

(95)

It is striking that the same pentapeptide serves as an activity - enhancing docking site for the methyltransferase CheR, the other enzyme involved in adaptational covalent modification of chemoreceptors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	It is striking that the same pentapeptide serves as an activity - enhancing docking site for the methyltransferase ProteinCheR , the other enzyme involved in adaptational covalent modification of chemoreceptors.

(96)

A shared docking site raises the tantalizing possibility that relative rates of methylation and demethylation could be influenced by competition between the two enzymes at that site.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(97)

Roles of cell division and gene transcription in the methylation of CpG islands.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(98)

De novo methylation of CpG islands within the promoters of eukaryotic genes is often associated with their transcriptional repression, yet the methylation of CpG islands located downstream of promoters does not block transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(99)

We investigated the kinetics of mRNA induction, demethylation, and remethylation of the p16 promoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.

Precision: 66.67

Recall: 85.71

F1: 75.00

Score	Text
0.9877	We investigated the kinetics of mRNA induction, demethylation, and remethylation of the Proteinp16 promoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
0.9588	We investigated the kinetics of mRNA induction, demethylation, and remethylation of the p16 Entitypromoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
0.9485	We investigated the kinetics of mRNA induction, demethylation, and remethylation of the p16 promoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island DNA_methylationmethylation and gene transcription.
0.9262	We investigated the kinetics of mRNA induction, demethylation, and DNA_methylationremethylation of the p16 promoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
0.7222	We investigated the kinetics of mRNA induction, demethylation, and remethylation of the p16 promoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between EntityCpG island methylation and gene transcription.
0.7088	We investigated the kinetics of mRNA induction, demethylation, and remethylation of the p16 promoter and Entitysecond - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
0.7960	We investigated the kinetics of mRNA induction, demethylation, and remethylation of the p16 promoter and second - exon EntityCpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
0.6599	We investigated the kinetics of mRNA induction, DNA_methylationdemethylation , and remethylation of the p16 promoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
0.5525	We investigated the kinetics of mRNA induction, demethylation, and remethylation of the p16 promoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between EntityCpG island methylation and gene transcription.
	We investigated the kinetics of mRNA induction, DNA_demethylationdemethylation , and remethylation of the p16 promoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.

(100)

The rates of remethylation of both CpG islands were associated with time but not with the rate of cell division, and remethylation of the p16 exon 2 CpG island occurred at a higher rate than that of the p16 promoter.

Precision: 62.50

Recall: 100.00

F1: 76.92

Score	Text
0.9813	The rates of remethylation of both CpG islands were associated with time but not with the rate of cell division, and remethylation of the p16 exon 2 CpG island occurred at a higher rate than that of the Proteinp16 promoter.
0.9664	The rates of remethylation of both CpG islands were associated with time but not with the rate of cell division, and DNA_methylationremethylation of the p16 exon 2 CpG island occurred at a higher rate than that of the p16 promoter.
0.9639	The rates of remethylation of both CpG islands were associated with time but not with the rate of cell division, and remethylation of the Proteinp16 exon 2 CpG island occurred at a higher rate than that of the p16 promoter.
0.9048	The rates of remethylation of both CpG islands were associated with time but not with the rate of cell division, and remethylation of the p16 exon 2 CpG island occurred at a higher rate than that of the p16 Entitypromoter .
0.5281	The rates of remethylation of both CpG islands were associated with time but not with the rate of cell division, and remethylation of the p16 Entityexon 2 CpG island occurred at a higher rate than that of the p16 promoter.
0.9509	The rates of DNA_methylationremethylation of both CpG islands were associated with time but not with the rate of cell division, and remethylation of the p16 exon 2 CpG island occurred at a higher rate than that of the p16 promoter.
0.6523	The rates of remethylation of both CpG islands were associated with time but not with the rate of cell division, and remethylation of the p16 exon 2 EntityCpG island occurred at a higher rate than that of the p16 promoter.
0.5021	The rates of remethylation of both EntityCpG islands were associated with time but not with the rate of cell division, and remethylation of the p16 exon 2 CpG island occurred at a higher rate than that of the p16 promoter.

(101)

We also examined the relationship between the remethylation of coding sequence CpG islands and gene transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(102)

The kinetics of remethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.

Precision: 83.33

Recall: 100.00

F1: 90.91

Score	Text
0.9825	The kinetics of DNA_methylationremethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.9814	The kinetics of remethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are DNA_methylationhypermethylated in T24 cells.
0.9756	The kinetics of remethylation of the Proteinp16 exon 2, PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.9712	The kinetics of remethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL exon 11, and ProteinMYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.9666	The kinetics of remethylation of the p16 exon 2, PAX - 6 exon 5, Proteinc - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.9610	The kinetics of remethylation of the p16 exon 2, ProteinPAX - 6 exon 5, c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.9118	The kinetics of remethylation of the p16 exon 2, PAX - 6 Entityexon 5 , c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.9040	The kinetics of remethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL Entityexon 11 , and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.8757	The kinetics of remethylation of the p16 Entityexon 2 , PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.8204	The kinetics of remethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 Entityexon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.5206	The kinetics of remethylation of the p16 exon 2, PAX - 6 Entityexon 5, c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.5121	The kinetics of remethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 Entityexon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.

(103)

Remethylation occurred most rapidly in the p16, PAX - 6, and c - ABL genes, shown to be transcribed prior to drug treatment.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9971	Remethylation occurred most rapidly in the p16, PAX - 6, and Proteinc - ABL genes, shown to be transcribed prior to drug treatment.
0.9960	Remethylation occurred most rapidly in the Proteinp16 , PAX - 6, and c - ABL genes, shown to be transcribed prior to drug treatment.
0.9942	Remethylation occurred most rapidly in the p16, ProteinPAX - 6 , and c - ABL genes, shown to be transcribed prior to drug treatment.
0.9563	DNA_methylationRemethylation occurred most rapidly in the p16, PAX - 6, and c - ABL genes, shown to be transcribed prior to drug treatment.

(104)

These regions also exhibited higher levels of remethylation in single - cell clones and subclones derived from 5 - Aza - CdR - treated T24 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9716	These regions also exhibited higher levels of DNA_methylationremethylation in single - cell clones and subclones derived from 5 - Aza - CdR - treated T24 cells.

(105)

Our data suggest that de novo methylation is not restricted to the S phase of the cell cycle and that transcription through CpG islands does not inhibit their remethylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(106)

Distinct methylation pattern and microsatellite instability in sporadic gastric cancer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(107)

Aberrant 5'CpG island methylation is an alternative mechanism of gene inactivation during the development of cancer as demonstrated for several tumor - suppressor genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(108)

Also, marked relationship of microsatellite instability ( MSI ) and DNA methylation has been reported in sporadic colorectal cancer, which is a result of epigenetic inactivation of hMLH1 in association of promoter hypermethylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9936	Also, marked relationship of microsatellite instability ( MSI ) and DNA methylation has been reported in sporadic colorectal cancer, which is a result of epigenetic inactivation of ProteinhMLH1 in association of promoter hypermethylation.
0.9859	Also, marked relationship of microsatellite instability ( MSI ) and DNA methylation has been reported in sporadic colorectal cancer, which is a result of epigenetic inactivation of hMLH1 in association of promoter DNA_methylationhypermethylation .
0.9826	Also, marked relationship of microsatellite instability ( MSI ) and DNA methylation has been reported in sporadic colorectal cancer, which is a result of epigenetic inactivation of hMLH1 in association of Entitypromoter hypermethylation.

(109)

In the present study, we investigated the 5'CpG island hypermethylation of hMLH1, E - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.

Precision: 62.50

Recall: 100.00

F1: 76.92

Score	Text
0.9943	In the present study, we investigated the 5'CpG island hypermethylation of hMLH1, E - cadherin and Proteinp16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.
0.9925	In the present study, we investigated the 5'CpG island hypermethylation of hMLH1, ProteinE - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.
0.9907	In the present study, we investigated the 5'CpG island DNA_methylationhypermethylation of hMLH1, E - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.
0.9872	In the present study, we investigated the 5'CpG island hypermethylation of ProteinhMLH1 , E - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.
0.8857	In the present study, we investigated the Entity5'CpG island hypermethylation of hMLH1, E - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.
0.8781	In the present study, we investigated the 5 ' EntityCpG island hypermethylation of hMLH1, E - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.
0.5562	In the present study, we investigated the Entity5'CpG island hypermethylation of hMLH1, E - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.
0.5354	In the present study, we investigated the 5 ' EntityCpG island hypermethylation of hMLH1, E - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.

(110)

Of 61 GCs investigated, 5 ( 8. 1 % ) tumors presented hMLH1 methylation, 16 ( 26. 2 % ) and 25 ( 40. 9 % ) showed E - cadherin and p16 methylation respectively, and 8 ( 13. 1 % ) presented high - frequency MSI ( MSI - H ).

Precision: 71.43

Recall: 100.00

F1: 83.33

Score	Text
0.9895	Of 61 GCs investigated, 5 ( 8. 1 % ) tumors presented hMLH1 methylation, 16 ( 26. 2 % ) and 25 ( 40. 9 % ) showed ProteinE - cadherin and p16 methylation respectively, and 8 ( 13. 1 % ) presented high - frequency MSI ( MSI - H ).
0.9870	Of 61 GCs investigated, 5 ( 8. 1 % ) tumors presented hMLH1 DNA_methylationmethylation , 16 ( 26. 2 % ) and 25 ( 40. 9 % ) showed E - cadherin and p16 methylation respectively, and 8 ( 13. 1 % ) presented high - frequency MSI ( MSI - H ).
0.9851	Of 61 GCs investigated, 5 ( 8. 1 % ) tumors presented ProteinhMLH1 methylation, 16 ( 26. 2 % ) and 25 ( 40. 9 % ) showed E - cadherin and p16 methylation respectively, and 8 ( 13. 1 % ) presented high - frequency MSI ( MSI - H ).
0.9844	Of 61 GCs investigated, 5 ( 8. 1 % ) tumors presented hMLH1 methylation, 16 ( 26. 2 % ) and 25 ( 40. 9 % ) showed E - cadherin and p16 DNA_methylationmethylation respectively, and 8 ( 13. 1 % ) presented high - frequency MSI ( MSI - H ).
0.9795	Of 61 GCs investigated, 5 ( 8. 1 % ) tumors presented hMLH1 methylation, 16 ( 26. 2 % ) and 25 ( 40. 9 % ) showed E - cadherin and Proteinp16 methylation respectively, and 8 ( 13. 1 % ) presented high - frequency MSI ( MSI - H ).
0.8182	Of 61 GCs investigated, 5 ( 8. 1 % ) tumors presented hMLH1 methylation, 16 ( 26. 2 % ) and 25 ( 40. 9 % ) showed E - cadherin and DNA_methylationp16 methylation respectively, and 8 ( 13. 1 % ) presented high - frequency MSI ( MSI - H ).
0.5782	Of 61 GCs investigated, 5 ( 8. 1 % ) tumors presented DNA_methylationhMLH1 methylation , 16 ( 26. 2 % ) and 25 ( 40. 9 % ) showed E - cadherin and p16 methylation respectively, and 8 ( 13. 1 % ) presented high - frequency MSI ( MSI - H ).

(111)

Of the 8 MSI - H patients, 5 presented hMLH1 methylation, whereas no low - frequency MSI ( MSI - L ) and microsatellite stable ( MSS ) cases exhibited hMLH1 methylation ( 5 / 8 vs. 0 / 43, p < 0. 00001 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	Of the 8 MSI - H patients, 5 presented ProteinhMLH1 methylation, whereas no low - frequency MSI ( MSI - L ) and microsatellite stable ( MSS ) cases exhibited hMLH1 methylation ( 5 / 8 vs. 0 / 43, p < 0. 00001 ).
0.9979	Of the 8 MSI - H patients, 5 presented hMLH1 methylation, whereas no low - frequency MSI ( MSI - L ) and microsatellite stable ( MSS ) cases exhibited ProteinhMLH1 methylation ( 5 / 8 vs. 0 / 43, p < 0. 00001 ).
0.9875	Of the 8 MSI - H patients, 5 presented hMLH1 methylation, whereas no low - frequency MSI ( MSI - L ) and microsatellite stable ( MSS ) cases exhibited hMLH1 DNA_methylationmethylation ( 5 / 8 vs. 0 / 43, p < 0. 00001 ).
0.9875	Of the 8 MSI - H patients, 5 presented hMLH1 DNA_methylationmethylation , whereas no low - frequency MSI ( MSI - L ) and microsatellite stable ( MSS ) cases exhibited hMLH1 methylation ( 5 / 8 vs. 0 / 43, p < 0. 00001 ).

(112)

Furthermore, these patients also presented E - cadherin and p16 hypermethylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9964	Furthermore, these patients also presented E - cadherin and Proteinp16 hypermethylation.
0.9959	Furthermore, these patients also presented ProteinE - cadherin and p16 hypermethylation.
0.9495	Furthermore, these patients also presented E - cadherin and p16 DNA_methylationhypermethylation .

(113)

Our data showed a significant correlation between hMLH1 methylation and MSI in GC, and suggested that a common mechanism of aberrant de novo methylation can be postulated in these cancers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9966	Our data showed a significant correlation between ProteinhMLH1 methylation and MSI in GC, and suggested that a common mechanism of aberrant de novo methylation can be postulated in these cancers.
0.9558	Our data showed a significant correlation between hMLH1 DNA_methylationmethylation and MSI in GC, and suggested that a common mechanism of aberrant de novo methylation can be postulated in these cancers.

(114)

Effect of HSP47 on prolyl 4 - hydroxylation of collagen model peptides.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9781	Effect of ProteinHSP47 on prolyl 4 - hydroxylation of collagen model peptides.

(115)

Prolyl 4 - hydroxylation, the most important post - translational modification in collagen biosynthesis, is catalyzed by prolyl 4 - hydroxylase, an endoplasmic reticulum - resident enzyme.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(116)

HSP47 is a collagen - binding stress protein which also resides in the endoplasmic reticulum ( Nagata, K. and Yamada, K. M. ( 1986 ) J.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	ProteinHSP47 is a collagen - binding stress protein which also resides in the endoplasmic reticulum ( Nagata, K. and Yamada, K. M. ( 1986 ) J.

(117)

Biol.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(118)

Chem., 261, 7531 - 7536 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(119)

Both prolyl 4 - hydroxylase and HSP47 interact with procollagen alpha - chains during their folding and / or modification in the endoplasmic reticulum.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9912	Both prolyl 4 - hydroxylase and ProteinHSP47 interact with procollagen alpha - chains during their folding and / or modification in the endoplasmic reticulum.

(120)

Recent study has revealed that a simple collagen model peptide, ( Pro - Pro - Gly ) n, is recognized by HSP47 as well as by prolyl 4 - hydroxylase in vitro ( Koide et al., manuscript submitted ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9815	Recent study has revealed that a simple collagen model peptide, ( Pro - Pro - Gly ) n, is recognized by ProteinHSP47 as well as by prolyl 4 - hydroxylase in vitro ( Koide et al., manuscript submitted ).

(121)

In the present study, we investigated the effect of HSP47 on the prolyl 4 - hydroxylation of such collagen model peptides.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9723	In the present study, we investigated the effect of ProteinHSP47 on the prolyl 4 - hydroxylation of such collagen model peptides.

(122)

To monitor the enzymatic hydroxylation of the peptides, we developed a non - RI assay system based on reversed - phase HPLC.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(123)

When HSP47 was added to the reaction mixture, substrate and less - hydroxylated materials accumulated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9884	When ProteinHSP47 was added to the reaction mixture, substrate and less - hydroxylated materials accumulated.

(124)

This effect depended on the peptide - binding activity of HSP47, because a mutant HSP47 without collagen - binding activity did not show any inhibitory effect on prolyl 4 - hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9971	This effect depended on the peptide - binding activity of HSP47, because a mutant ProteinHSP47 without collagen - binding activity did not show any inhibitory effect on prolyl 4 - hydroxylation.
0.9959	This effect depended on the peptide - binding activity of ProteinHSP47 , because a mutant HSP47 without collagen - binding activity did not show any inhibitory effect on prolyl 4 - hydroxylation.

(125)

Kinetic analysis and other biochemical analyses suggest that HSP47 retards the enzymatic reaction competing for the substrate peptide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9830	Kinetic analysis and other biochemical analyses suggest that ProteinHSP47 retards the enzymatic reaction competing for the substrate peptide.

(126)

Mouse K - Cl cotransporter KCC1 : cloning, mapping, pathological expression, and functional regulation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	Mouse K - Cl cotransporter ProteinKCC1 : cloning, mapping, pathological expression, and functional regulation.

(127)

Although K - Cl cotransporter ( KCC1 ) mRNA is expressed in many tissues, K - Cl cotransport activity has been measured in few cell types, and detection of endogenous KCC1 polypeptide has not yet been reported.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	Although K - Cl cotransporter ( KCC1 ) mRNA is expressed in many tissues, K - Cl cotransport activity has been measured in few cell types, and detection of endogenous ProteinKCC1 polypeptide has not yet been reported.
0.9807	Although K - Cl cotransporter Protein( KCC1 ) mRNA is expressed in many tissues, K - Cl cotransport activity has been measured in few cell types, and detection of endogenous KCC1 polypeptide has not yet been reported.

(128)

We have cloned the mouse erythroid KCC1 ( mKCC1 ) cDNA and its flanking genomic regions and mapped the mKCC1 gene to chromosome 8.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9998	We have cloned the mouse erythroid KCC1 ( mKCC1 ) cDNA and its flanking genomic regions and mapped the ProteinmKCC1 gene to chromosome 8.
0.9816	We have cloned the mouse erythroid KCC1 Protein( mKCC1 ) cDNA and its flanking genomic regions and mapped the mKCC1 gene to chromosome 8.
0.9722	We have cloned the mouse erythroid ProteinKCC1 ( mKCC1 ) cDNA and its flanking genomic regions and mapped the mKCC1 gene to chromosome 8.
0.8525	We have cloned the mouse erythroid ProteinKCC1 ( mKCC1 ) cDNA and its flanking genomic regions and mapped the mKCC1 gene to chromosome 8.

(129)

Three anti - peptide antibodies raised against recombinant mKCC1 function as immunoblot and immunoprecipitation reagents.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Three anti - peptide antibodies raised against recombinant ProteinmKCC1 function as immunoblot and immunoprecipitation reagents.

(130)

The tissue distributions of mKCC1 mRNA and protein are widespread, and mKCC1 RNA is constitutively expressed during erythroid differentiation of ES cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	The tissue distributions of mKCC1 mRNA and protein are widespread, and ProteinmKCC1 RNA is constitutively expressed during erythroid differentiation of ES cells.
0.9997	The tissue distributions of ProteinmKCC1 mRNA and protein are widespread, and mKCC1 RNA is constitutively expressed during erythroid differentiation of ES cells.

(131)

KCC1 polypeptide or related antigen is present in erythrocytes of multiple species in which K - Cl cotransport activity has been documented.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	ProteinKCC1 polypeptide or related antigen is present in erythrocytes of multiple species in which K - Cl cotransport activity has been documented.

(132)

Erythroid KCC1 polypeptide abundance is elevated in proportion to reticulocyte counts in density - fractionated cells, in bleeding - induced reticulocytosis, in mouse models of sickle cell disease and thalassemia, and in the corresponding human disorders. mKCC1 - mediated uptake of ( 86 ) Rb into Xenopus oocytes requires extracellular Cl ( - ), is blocked by the diuretic R ( + ) - [ 2 - n - butyl - 6, 7 - dichloro - 2 - cyclopentyl - 2, 3 - dihydro - 1 - oxo - 1H - indenyl - 5 - yl - ) oxy ] acetic acid, and exhibits an erythroid pattern of acute regulation, with activation by hypotonic swelling, N - ethylmaleimide, and staurosporine and inhibition by calyculin and okadaic acid.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9978	Erythroid ProteinKCC1 polypeptide abundance is elevated in proportion to reticulocyte counts in density - fractionated cells, in bleeding - induced reticulocytosis, in mouse models of sickle cell disease and thalassemia, and in the corresponding human disorders. mKCC1 - mediated uptake of ( 86 ) Rb into Xenopus oocytes requires extracellular Cl ( - ), is blocked by the diuretic R ( + ) - [ 2 - n - butyl - 6, 7 - dichloro - 2 - cyclopentyl - 2, 3 - dihydro - 1 - oxo - 1H - indenyl - 5 - yl - ) oxy ] acetic acid, and exhibits an erythroid pattern of acute regulation, with activation by hypotonic swelling, N - ethylmaleimide, and staurosporine and inhibition by calyculin and okadaic acid.
0.9933	Erythroid KCC1 polypeptide abundance is elevated in proportion to reticulocyte counts in density - fractionated cells, in bleeding - induced reticulocytosis, in mouse models of sickle cell disease and thalassemia, and in the corresponding human disorders. ProteinmKCC1 - mediated uptake of ( 86 ) Rb into Xenopus oocytes requires extracellular Cl ( - ), is blocked by the diuretic R ( + ) - [ 2 - n - butyl - 6, 7 - dichloro - 2 - cyclopentyl - 2, 3 - dihydro - 1 - oxo - 1H - indenyl - 5 - yl - ) oxy ] acetic acid, and exhibits an erythroid pattern of acute regulation, with activation by hypotonic swelling, N - ethylmaleimide, and staurosporine and inhibition by calyculin and okadaic acid.

(133)

These reagents and findings will expedite studies of KCC1 structure - function relationships and of the pathobiology of KCC1 - mediated K - Cl cotransport.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	These reagents and findings will expedite studies of ProteinKCC1 structure - function relationships and of the pathobiology of KCC1 - mediated K - Cl cotransport.
0.9959	These reagents and findings will expedite studies of KCC1 structure - function relationships and of the pathobiology of ProteinKCC1 - mediated K - Cl cotransport.

(134)

Progesterone metabolism in the human kidney and inhibition of 11beta - hydroxysteroid dehydrogenase type 2 by progesterone and its metabolites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.8841	Progesterone metabolism in the human kidney and inhibition of Protein11beta - hydroxysteroid dehydrogenase type 2 by progesterone and its metabolites.

(135)

Progesterone binds with high affinity to the mineralocorticoid ( MC ) receptor, but confers only very low agonistic MC activity.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.8592	Progesterone binds with high affinity to the Proteinmineralocorticoid ( MC ) receptor , but confers only very low agonistic MC activity.
0.6067	Progesterone binds with high affinity to the mineralocorticoid Protein( MC ) receptor , but confers only very low agonistic MC activity.

(136)

Therefore, progesterone is a potent MC antagonist in vitro.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(137)

Although progesterone reaches up to 100 times higher plasma levels in late pregnancy than aldosterone, the in vivo MC antagonistic effect of progesterone seems to be relatively weak.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(138)

One explanation for this phenomenon could be local metabolism of progesterone in the human kidney, similar to the inactivation of cortisol to cortisone by the 11beta - hydroxysteroid dehydrogenase ( 11beta - HSD ) type 2.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
	One explanation for this phenomenon could be local metabolism of progesterone in the human kidney, similar to the inactivation of cortisol to cortisone by the Protein11beta - hydroxysteroid dehydrogenase ( 11beta - HSD ) type 2 .

(139)

We studied the metabolism of progesterone in the human kidney in vitro and found reduction to 20alpha - dihydro ( DH ) - progesterone as the main metabolite.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(140)

Ring - A reduction to 5alpha - DH - progesterone, 20alpha - DH - 5alpha - DH - progesterone, and 3beta, 5alpha - tetrahydro ( TH ) - progesterone was also documented.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(141)

We further showed for the first time that 17 - hydroxylation of progesterone ( 17alpha - OH - progesterone, 17alpha - OH, 20alpha - DH - progesterone ), normally localized in the adrenals and the gonads, occurs in the human adult kidney.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(142)

We found no formation of deoxycorticosterone from progesterone in the human adult kidney.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(143)

Using human kidney cortex microsomes, we tested the inhibitory potency of progesterone and its metabolites on the 11beta - HSD type 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.8784	Using human kidney cortex microsomes, we tested the inhibitory potency of progesterone and its metabolites on the Protein11beta - HSD type 2 .

(144)

The most potent inhibitor was progesterone itself ( IC50 = 4. 8 x 10 ( - 8 ) mol / L ), followed by 5alpha - DH - progesterone ( IC50 = 2. 4 x 10 ( - 7 ) mol / L ), 20alpha - DH - progesterone, 3beta, 5alpha - TH - progesterone, 17alpha - OH - progesterone, and 20alpha - DH - 5alpha - DH - progesterone ( IC50 between 7. 7 x 10 ( - 7 ) mol / L and 1. 3 x 10 ( - 6 ) mol / L ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(145)

The least potent inhibitor was 17alpha - OH, 20alpha - DH - progesterone.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(146)

In addition to progesterone metabolism by the kidney, the inhibition of 11beta - HSD type 2 by progesterone and its metabolites could be a second explanation for the weak MC - antagonist activity of progesterone in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9603	In addition to progesterone metabolism by the kidney, the inhibition of Protein11beta - HSD type 2 by progesterone and its metabolites could be a second explanation for the weak MC - antagonist activity of progesterone in vivo.

(147)

Inhibition of 11beta - HSD type 2 leads to an increase of intracellular cortisol in a way that the local equilibrium between the MC agonist cortisol and the antagonist progesterone is shifted to the agonist side.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9710	Inhibition of Protein11beta - HSD type 2 leads to an increase of intracellular cortisol in a way that the local equilibrium between the MC agonist cortisol and the antagonist progesterone is shifted to the agonist side.

(148)

Deglycosylation of a bifunctional lutropin - follitropin agonist reduced its follitropin activity more than its lutropin activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(149)

OBJECTIVE : To design a drug that blocks the gonadal actions of lutropins and follitropins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(150)

DESIGN : Controlled in vitro study.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(151)

SETTING : Academic laboratory.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(152)

PATIENT ( S ) : None.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(153)

INTERVENTION ( S ) : We removed three glycosylation signals from an hCG - hFSH chimera known to have high affinity for LH and FSH receptors, expecting this would create a bifunctional antagonist ( dgCFC ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9611	INTERVENTION ( S ) : We removed three glycosylation signals from an hCG - hFSH chimera known to have high affinity for LH and ProteinFSH receptors , expecting this would create a bifunctional antagonist ( dgCFC ).
0.9513	INTERVENTION ( S ) : We removed three glycosylation signals from an hCG - hFSH chimera known to have high affinity for ProteinLH and FSH receptors, expecting this would create a bifunctional antagonist ( dgCFC ).

(154)

To offset the inhibition of subunit combination caused by deglycosylation of alpha - subunit loop 2, we prepared dgCFC as a single - chain fusion protein containing the alpha - subunit downstream of the chimeric beta - subunit.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(155)

MAIN OUTCOME MEASURE ( S ) : Receptor binding, cyclic adenosine monophosphate accumulation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(156)

RESULT ( S ) : dgCFC bound LH or FSH receptors similar to hCG or hFSH.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9893	RESULT ( S ) : dgCFC bound LH or ProteinFSH receptors similar to hCG or hFSH.
0.9818	RESULT ( S ) : dgCFC bound ProteinLH or FSH receptors similar to hCG or hFSH.

(157)

It was a partial agonist and had one tenth the efficacy of hFSH and two thirds the efficacy of hCG.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(158)

CONCLUSION ( S ) : The surprising high residual lutropin activity of dgCFC indicated that its FSH residues offset the effects of deglycosylation, suggesting this approach to preparing a bifunctional antagonist is unlikely to lead to a useful drug.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(159)

The increased lutropin efficacy of dgCFC relative to deglycosylated hCG supports the idea that oligosaccharides modulate glycoprotein hormone efficacy through an influence on hormone conformation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(160)

Regulation of thyrotropin receptor protein expression in insect cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9476	Regulation of Proteinthyrotropin receptor protein expression in insect cells.

(161)

Expression of large quantities of conformationally intact thyrotropin receptor ( TSHR ) is essential to understand the structure - function relationship of the receptor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9694	Expression of large quantities of conformationally intact thyrotropin receptor Protein( TSHR ) is essential to understand the structure - function relationship of the receptor.
0.9445	Expression of large quantities of conformationally intact Proteinthyrotropin receptor ( TSHR ) is essential to understand the structure - function relationship of the receptor.

(162)

We expressed three different constructs of full - length human TSHR in insect cells : ( a ) a TSHR cDNA lacking signal sequence ( TSHR - ns ), ( b ) a TSHR cDNA containing human TSHR signal sequence ( TSHR - hs ) and ( c ) a TSHR cDNA with baculovirus envelope protein encoded signal sequence gp - 67 ( TSHR - gp ).

Precision: 88.89

Recall: 100.00

F1: 94.12

Score	Text
0.9993	We expressed three different constructs of full - length human ProteinTSHR in insect cells : ( a ) a TSHR cDNA lacking signal sequence ( TSHR - ns ), ( b ) a TSHR cDNA containing human TSHR signal sequence ( TSHR - hs ) and ( c ) a TSHR cDNA with baculovirus envelope protein encoded signal sequence gp - 67 ( TSHR - gp ).
0.9984	We expressed three different constructs of full - length human TSHR in insect cells : ( a ) a TSHR cDNA lacking signal sequence ( TSHR - ns ), ( b ) a TSHR cDNA containing human TSHR signal sequence ( TSHR - hs ) and ( c ) a ProteinTSHR cDNA with baculovirus envelope protein encoded signal sequence gp - 67 ( TSHR - gp ).
0.9972	We expressed three different constructs of full - length human TSHR in insect cells : ( a ) a ProteinTSHR cDNA lacking signal sequence ( TSHR - ns ), ( b ) a TSHR cDNA containing human TSHR signal sequence ( TSHR - hs ) and ( c ) a TSHR cDNA with baculovirus envelope protein encoded signal sequence gp - 67 ( TSHR - gp ).
0.9963	We expressed three different constructs of full - length human TSHR in insect cells : ( a ) a TSHR cDNA lacking signal sequence ( TSHR - ns ), ( b ) a TSHR cDNA containing human ProteinTSHR signal sequence ( TSHR - hs ) and ( c ) a TSHR cDNA with baculovirus envelope protein encoded signal sequence gp - 67 ( TSHR - gp ).
0.9963	We expressed three different constructs of full - length human TSHR in insect cells : ( a ) a TSHR cDNA lacking signal sequence ( TSHR - ns ), ( b ) a ProteinTSHR cDNA containing human TSHR signal sequence ( TSHR - hs ) and ( c ) a TSHR cDNA with baculovirus envelope protein encoded signal sequence gp - 67 ( TSHR - gp ).
0.9937	We expressed three different constructs of full - length human TSHR in insect cells : ( a ) a TSHR cDNA lacking signal sequence ( TSHR - ns ), ( b ) a TSHR cDNA containing human TSHR signal sequence Protein( TSHR - hs ) and ( c ) a TSHR cDNA with baculovirus envelope protein encoded signal sequence gp - 67 ( TSHR - gp ).
0.9901	We expressed three different constructs of full - length human TSHR in insect cells : ( a ) a TSHR cDNA lacking signal sequence Protein( TSHR - ns ) , ( b ) a TSHR cDNA containing human TSHR signal sequence ( TSHR - hs ) and ( c ) a TSHR cDNA with baculovirus envelope protein encoded signal sequence gp - 67 ( TSHR - gp ).
0.9901	We expressed three different constructs of full - length human TSHR in insect cells : ( a ) a TSHR cDNA lacking signal sequence ( TSHR - ns ), ( b ) a TSHR cDNA containing human TSHR signal sequence ( TSHR - hs ) and ( c ) a TSHR cDNA with baculovirus envelope protein encoded signal sequence gp - 67 Protein( TSHR - gp ) .
0.7322	We expressed three different constructs of full - length human TSHR in insect cells : ( a ) a TSHR cDNA lacking signal sequence ( TSHR - ns ), ( b ) a TSHR cDNA containing human TSHR signal sequence ( TSHR - hs ) and ( c ) a TSHR cDNA with baculovirus envelope protein encoded signal sequence Proteingp - 67 ( TSHR - gp ).

(163)

No unique protein band, corresponding to any of these recombinant proteins, was visible upon Coomassie Blue staining after SDS - PAGE.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(164)

However, Western blot using TSHR specific monoclonal antibody showed unique bands around 80, 100 and 100 kDa in TSHR - ns, TSHR - hs and TSHR - gp virus infected insect cells respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	However, Western blot using TSHR specific monoclonal antibody showed unique bands around 80, 100 and 100 kDa in TSHR - ns, ProteinTSHR - hs and TSHR - gp virus infected insect cells respectively.
0.9996	However, Western blot using TSHR specific monoclonal antibody showed unique bands around 80, 100 and 100 kDa in ProteinTSHR - ns , TSHR - hs and TSHR - gp virus infected insect cells respectively.
0.9994	However, Western blot using TSHR specific monoclonal antibody showed unique bands around 80, 100 and 100 kDa in TSHR - ns, TSHR - hs and ProteinTSHR - gp virus infected insect cells respectively.
0.9989	However, Western blot using ProteinTSHR specific monoclonal antibody showed unique bands around 80, 100 and 100 kDa in TSHR - ns, TSHR - hs and TSHR - gp virus infected insect cells respectively.

(165)

All three full - length TSHR proteins could neutralize the TSH binding inhibitory immunoglobulin ( TBII ) activity from sera of experimental animals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	All three full - length ProteinTSHR proteins could neutralize the TSH binding inhibitory immunoglobulin ( TBII ) activity from sera of experimental animals.

(166)

However, only glycosylated proteins ( TSHR - hs and TSHR - gp ) neutralized the TBII activity of sera from autoimmune thyroid patients, confirming the importance of glycosylation for patient autoantibody reactivity.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9993	However, only glycosylated proteins ( TSHR - hs and ProteinTSHR - gp ) neutralized the TBII activity of sera from autoimmune thyroid patients, confirming the importance of glycosylation for patient autoantibody reactivity.
0.9981	However, only glycosylated proteins Protein( TSHR - hs and TSHR - gp ) neutralized the TBII activity of sera from autoimmune thyroid patients, confirming the importance of glycosylation for patient autoantibody reactivity.
0.9720	However, only Glycosylationglycosylated proteins ( TSHR - hs and TSHR - gp ) neutralized the TBII activity of sera from autoimmune thyroid patients, confirming the importance of glycosylation for patient autoantibody reactivity.
0.8409	However, only glycosylated proteins ( TSHR - hs and TSHR - gp ) neutralized the TBII activity of sera from autoimmune thyroid patients, confirming the importance of Glycosylationglycosylation for patient autoantibody reactivity.

(167)

Expression levels of full - length TSHR proteins were much lower than the levels of similarly produced corresponding ectodomains of TSHR proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	Expression levels of full - length ProteinTSHR proteins were much lower than the levels of similarly produced corresponding ectodomains of TSHR proteins.
0.9990	Expression levels of full - length TSHR proteins were much lower than the levels of similarly produced corresponding ectodomains of ProteinTSHR proteins.

(168)

Southern blot and Northern blot analyses showed that DNA and RNA levels in full - length TSHR virus infected insect cells were comparable to the levels found in cells infected with viruses encoding only the ectodomain of TSHR.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Southern blot and Northern blot analyses showed that DNA and RNA levels in full - length TSHR virus infected insect cells were comparable to the levels found in cells infected with viruses encoding only the ectodomain of ProteinTSHR .

(169)

These data suggest that full - length TSHR expression is very low and is regulated at the translational level.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	These data suggest that full - length ProteinTSHR expression is very low and is regulated at the translational level.

(170)

BasT, a membrane - bound transducer protein for amino acid detection in Halobacterium salinarum.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	ProteinBasT , a membrane - bound transducer protein for amino acid detection in Halobacterium salinarum.

(171)

Halophilic archaea, such as eubacteria, use methyl - accepting chemotaxis proteins ( MCPs ) to sense their environment.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(172)

We show here that BasT is a halobacterial transducer protein ( Htp ) responsible for chemotaxis towards five attractant amino acids.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	We show here that ProteinBasT is a halobacterial transducer protein ( Htp ) responsible for chemotaxis towards five attractant amino acids.

(173)

The C - terminus of the protein exhibits the highly conserved regions that are diagnostic for MCPs : the signalling domain for communication with the histidine kinase and the methylation sites that interact with the methylation / demethylation enzymes for adaptation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(174)

Hydropathy analysis predicts an enterobacterial - type transducer protein topology for BasT, with an extracellular putative ligand - binding domain flanked by two transmembrane helices and a cytoplasmic domain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Hydropathy analysis predicts an enterobacterial - type transducer protein topology for ProteinBasT , with an extracellular putative ligand - binding domain flanked by two transmembrane helices and a cytoplasmic domain.

(175)

BasT - inactivated mutant cells are missing a membrane protein radiolabelled with L - [ methyl - 3H ] - methionine in wild - type cells, confirming that BasT is methylatable and membrane bound.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	ProteinBasT - inactivated mutant cells are missing a membrane protein radiolabelled with L - [ methyl - 3H ] - methionine in wild - type cells, confirming that BasT is methylatable and membrane bound.
0.9986	BasT - inactivated mutant cells are missing a membrane protein radiolabelled with L - [ methyl - 3H ] - methionine in wild - type cells, confirming that ProteinBasT is methylatable and membrane bound.
0.8197	BasT - inactivated mutant cells are missing a membrane protein radiolabelled with L - [ methyl - 3H ] - methionine in wild - type cells, confirming that BasT is Methylationmethylatable and membrane bound.

(176)

Behavioural analysis of the basT mutant cells by capillary and chemical - in - plug assays demonstrates complete loss of chemotactic responses towards five ( leucine, isoleucine, valine, methionine and cysteine ) of the six attractant amino acids for Halobacterium salinarum, whereas they still respond to arginine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	Behavioural analysis of the ProteinbasT mutant cells by capillary and chemical - in - plug assays demonstrates complete loss of chemotactic responses towards five ( leucine, isoleucine, valine, methionine and cysteine ) of the six attractant amino acids for Halobacterium salinarum, whereas they still respond to arginine.

(177)

The volatile methyl group production assays also corroborate these findings and confirm that BasT signalling induces methyl group turnover.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	The volatile methyl group production assays also corroborate these findings and confirm that ProteinBasT signalling induces methyl group turnover.

(178)

Our data identify BasT as the chemotaxis transducer protein for the branched chain amino acids leucine, isoleucine and valine as well as for methionine and cysteine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Our data identify ProteinBasT as the chemotaxis transducer protein for the branched chain amino acids leucine, isoleucine and valine as well as for methionine and cysteine.

(179)

Thus, BasT and the arginine sensor Car cover the entire spectrum of chemotactic responses towards attractant amino acids in H. salinarum.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Thus, ProteinBasT and the arginine sensor Car cover the entire spectrum of chemotactic responses towards attractant amino acids in H. salinarum.
0.9991	Thus, BasT and the arginine sensor ProteinCar cover the entire spectrum of chemotactic responses towards attractant amino acids in H. salinarum.

(180)

Additional N - glycosylation at Asn ( 13 ) rescues the human LHbeta - subunit from disulfide - linked aggregation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9937	Additional N - glycosylation at Asn ( 13 ) rescues the human ProteinLHbeta - subunit from disulfide - linked aggregation.
0.9927	Additional GlycosylationN - glycosylation at Asn ( 13 ) rescues the human LHbeta - subunit from disulfide - linked aggregation.
0.9679	Additional N - glycosylation at EntityAsn ( 13 ) rescues the human LHbeta - subunit from disulfide - linked aggregation.

(181)

CG, LH, FSH, and TSH are a family of heterodimeric glycoprotein hormones that contain a common alpha - subunit, but differ in their hormone - specific beta - subunits.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(182)

Despite the considerable homology between LHbeta and CGbeta, we previously demonstrated that, when expressed in GH ( 3 ) cells, the secreted form of LHbeta showed mispaired disulfide - linked aggregation in addition to monomer, whereas no aggregation was observed in CGbeta.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Despite the considerable homology between LHbeta and CGbeta, we previously demonstrated that, when expressed in GH ( 3 ) cells, the secreted form of ProteinLHbeta showed mispaired disulfide - linked aggregation in addition to monomer, whereas no aggregation was observed in CGbeta.
0.9998	Despite the considerable homology between ProteinLHbeta and CGbeta, we previously demonstrated that, when expressed in GH ( 3 ) cells, the secreted form of LHbeta showed mispaired disulfide - linked aggregation in addition to monomer, whereas no aggregation was observed in CGbeta.
0.9998	Despite the considerable homology between LHbeta and ProteinCGbeta , we previously demonstrated that, when expressed in GH ( 3 ) cells, the secreted form of LHbeta showed mispaired disulfide - linked aggregation in addition to monomer, whereas no aggregation was observed in CGbeta.
0.9997	Despite the considerable homology between LHbeta and CGbeta, we previously demonstrated that, when expressed in GH ( 3 ) cells, the secreted form of LHbeta showed mispaired disulfide - linked aggregation in addition to monomer, whereas no aggregation was observed in ProteinCGbeta .

(183)

To determine the domains which are associated with the LHbeta - aggregation and which prevent CGbeta - aggregation, mutant beta - subunits in glycosylation and carboxy - terminus were expressed in GH ( 3 ) cells, and the occurrence of aggregation was assessed by continuous labeling with [ 35S ] methionine / cysteine, immunoprecipitation with anti - hCGbeta serum, and sodium dodecyl sulfate - polyacrylamide gel electrophoresis in a non - reducing condition.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9996	To determine the domains which are associated with the ProteinLHbeta - aggregation and which prevent CGbeta - aggregation, mutant beta - subunits in glycosylation and carboxy - terminus were expressed in GH ( 3 ) cells, and the occurrence of aggregation was assessed by continuous labeling with [ 35S ] methionine / cysteine, immunoprecipitation with anti - hCGbeta serum, and sodium dodecyl sulfate - polyacrylamide gel electrophoresis in a non - reducing condition.
0.9994	To determine the domains which are associated with the LHbeta - aggregation and which prevent ProteinCGbeta - aggregation , mutant beta - subunits in glycosylation and carboxy - terminus were expressed in GH ( 3 ) cells, and the occurrence of aggregation was assessed by continuous labeling with [ 35S ] methionine / cysteine, immunoprecipitation with anti - hCGbeta serum, and sodium dodecyl sulfate - polyacrylamide gel electrophoresis in a non - reducing condition.
0.9960	To determine the domains which are associated with the LHbeta - aggregation and which prevent CGbeta - aggregation, mutant beta - subunits in glycosylation and carboxy - terminus were expressed in GH ( 3 ) cells, and the occurrence of aggregation was assessed by continuous labeling with [ 35S ] methionine / cysteine, immunoprecipitation with Proteinanti - hCGbeta serum, and sodium dodecyl sulfate - polyacrylamide gel electrophoresis in a non - reducing condition.
0.5586	To determine the domains which are associated with the LHbeta - aggregation and which prevent CGbeta - aggregation, mutant beta - subunits in Glycosylationglycosylation and carboxy - terminus were expressed in GH ( 3 ) cells, and the occurrence of aggregation was assessed by continuous labeling with [ 35S ] methionine / cysteine, immunoprecipitation with anti - hCGbeta serum, and sodium dodecyl sulfate - polyacrylamide gel electrophoresis in a non - reducing condition.

(184)

No aggregation was seen when N - linked oligosaccharides were attached to the Asn ( 13 ) of LHbeta.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9873	No aggregation was seen when N - linked oligosaccharides were attached to the Asn ( 13 ) of ProteinLHbeta .
0.9754	No aggregation was seen when N - linked oligosaccharides were attached to the EntityAsn ( 13 ) of LHbeta.
0.9257	No aggregation was seen when N - linked Entityoligosaccharides were attached to the Asn ( 13 ) of LHbeta.
0.6661	No aggregation was seen when N - linked oligosaccharides were Glycosylationattached to the Asn ( 13 ) of LHbeta.

(185)

Removal of the carbohydrate unit at the Asn ( 13 ) of CGbeta caused aggregation, although the amount was less than 10 % of monomer.

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9914	Removal of the carbohydrate unit at the EntityAsn ( 13 ) of CGbeta caused aggregation, although the amount was less than 10 % of monomer.
0.9859	Removal of the carbohydrate unit at the Asn ( 13 ) of ProteinCGbeta caused aggregation, although the amount was less than 10 % of monomer.
0.8673	Removal of the Entitycarbohydrate unit at the Asn ( 13 ) of CGbeta caused aggregation, although the amount was less than 10 % of monomer.
0.4781	DeglycosylationRemoval of the carbohydrate unit at the Asn ( 13 ) of CGbeta caused aggregation, although the amount was less than 10 % of monomer.
0.8217	Removal of the Entitycarbohydrate unit at the Asn ( 13 ) of CGbeta caused aggregation, although the amount was less than 10 % of monomer.

(186)

The carboxy - terminal regions of neither LHbeta nor CGbeta were associated with their aggregation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	The carboxy - terminal regions of neither LHbeta nor ProteinCGbeta were associated with their aggregation.
0.9982	The carboxy - terminal regions of neither ProteinLHbeta nor CGbeta were associated with their aggregation.

(187)

Both CGbeta wild - type ( WT ) and CGbeta lacking N - glycosylation at Asn ( 13 ) ( CGbeta - N13 ) showed aggregates in lysate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	Both CGbeta wild - type ( WT ) and ProteinCGbeta lacking N - glycosylation at Asn ( 13 ) ( CGbeta - N13 ) showed aggregates in lysate.
0.9969	Both ProteinCGbeta wild - type ( WT ) and CGbeta lacking N - glycosylation at Asn ( 13 ) ( CGbeta - N13 ) showed aggregates in lysate.
0.9872	Both CGbeta wild - type ( WT ) and CGbeta lacking GlycosylationN - glycosylation at Asn ( 13 ) ( CGbeta - N13 ) showed aggregates in lysate.
0.9241	Both CGbeta wild - type ( WT ) and CGbeta lacking N - glycosylation at EntityAsn ( 13 ) ( CGbeta - N13 ) showed aggregates in lysate.

(188)

However, in contrast to CGbeta - N13, CGbetaWT revealed no aggregation in medium.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	However, in contrast to CGbeta - N13, ProteinCGbetaWT revealed no aggregation in medium.
0.9984	However, in contrast to ProteinCGbeta - N13 , CGbetaWT revealed no aggregation in medium.

(189)

These results indicate that the backbone structure consisting of 114 amino acids and N - linked glycosylation at Asn ( 30 ) is involved in the aggregation of LHbeta.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9966	These results indicate that the backbone structure consisting of 114 amino acids and N - linked glycosylation at Asn ( 30 ) is involved in the aggregation of ProteinLHbeta .
0.9745	These results indicate that the backbone structure consisting of 114 amino acids and N - linked glycosylation at EntityAsn ( 30 ) is involved in the aggregation of LHbeta.
0.8888	These results indicate that the backbone structure consisting of 114 amino acids and GlycosylationN - linked glycosylation at Asn ( 30 ) is involved in the aggregation of LHbeta.
0.9807	These results indicate that the backbone structure consisting of 114 amino acids and N - linked Glycosylationglycosylation at Asn ( 30 ) is involved in the aggregation of LHbeta.

(190)

Moreover, N - glycosylation at Asn ( 13 ) does not prevent such aggregation, but instead plays an important role in correct folding for both LHbeta - and CGbeta - subunits to be secreted as monomer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9965	Moreover, N - glycosylation at Asn ( 13 ) does not prevent such aggregation, but instead plays an important role in correct folding for both ProteinLHbeta - and CGbeta - subunits to be secreted as monomer.
0.9960	Moreover, N - glycosylation at Asn ( 13 ) does not prevent such aggregation, but instead plays an important role in correct folding for both LHbeta - and ProteinCGbeta - subunits to be secreted as monomer.
0.9920	Moreover, GlycosylationN - glycosylation at Asn ( 13 ) does not prevent such aggregation, but instead plays an important role in correct folding for both LHbeta - and CGbeta - subunits to be secreted as monomer.
0.9863	Moreover, N - glycosylation at EntityAsn ( 13 ) does not prevent such aggregation, but instead plays an important role in correct folding for both LHbeta - and CGbeta - subunits to be secreted as monomer.

(191)

Type XIII collagen forms homotrimers with three triple helical collagenous domains and its association into disulfide - bonded trimers is enhanced by prolyl 4 - hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9499	ProteinType XIII collagen forms homotrimers with three triple helical collagenous domains and its association into disulfide - bonded trimers is enhanced by prolyl 4 - hydroxylase.

(192)

Type XIII collagen is a type II transmembrane protein predicted to consist of a short cytosolic domain, a single transmembrane domain, and three collagenous domains flanked by noncollagenous sequences.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9699	ProteinType XIII collagen is a type II transmembrane protein predicted to consist of a short cytosolic domain, a single transmembrane domain, and three collagenous domains flanked by noncollagenous sequences.
0.6768	Type ProteinXIII collagen is a type II transmembrane protein predicted to consist of a short cytosolic domain, a single transmembrane domain, and three collagenous domains flanked by noncollagenous sequences.

(193)

Previous studies on mRNAs indicate that the structures of the collagenous domain closest to the cell membrane, COL1, the adjacent noncollagenous domain, NC2, and the C - terminal domains COL3 and NC4 are subject to alternative splicing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(194)

In order to extend studies of type XIII collagen from cDNAs to the protein level we have produced it in insect cells by means of baculoviruses.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9431	In order to extend studies of Proteintype XIII collagen from cDNAs to the protein level we have produced it in insect cells by means of baculoviruses.
0.5934	In order to extend studies of type ProteinXIII collagen from cDNAs to the protein level we have produced it in insect cells by means of baculoviruses.

(195)

Type XIII collagen alpha chains were found to associate into disulfide - bonded trimers, and hydroxylation of proline residues dramatically enhanced this association.

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Text
0.9727	Type XIII collagen alpha chains were found to associate into disulfide - bonded trimers, and Hydroxylationhydroxylation of proline residues dramatically enhanced this association.
0.9044	Type XIII collagen alpha chains were found to associate into disulfide - bonded trimers, and hydroxylation of Entityproline residues dramatically enhanced this association.
0.7708	ProteinType XIII collagen alpha chains were found to associate into disulfide - bonded trimers, and hydroxylation of proline residues dramatically enhanced this association.
0.7303	Type XIII collagen alpha chains were found to associate into disulfide - bonded trimers, and hydroxylation of Entityproline residues dramatically enhanced this association.
0.6210	ProteinType XIII collagen alpha chains were found to associate into disulfide - bonded trimers, and hydroxylation of proline residues dramatically enhanced this association.

(196)

This protein contains altogether eight cysteine residues, and interchain disulfide bonds could be located in the NC1 domain and possibly at the junction of COL1 and NC2, while the two cysteine residues in NC4 are likely to form intrachain bonds.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(197)

Pepsin and trypsin / chymotrypsin digestions indicated that the type XIII collagen alpha chains form homotrimers whose three collagenous domains are in triple helical conformation.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9968	ProteinPepsin and trypsin / chymotrypsin digestions indicated that the type XIII collagen alpha chains form homotrimers whose three collagenous domains are in triple helical conformation.
0.7930	Pepsin and trypsin / chymotrypsin digestions indicated that the Proteintype XIII collagen alpha chains form homotrimers whose three collagenous domains are in triple helical conformation.
0.6860	Pepsin and trypsin / chymotrypsin digestions indicated that the Proteintype XIII collagen alpha chains form homotrimers whose three collagenous domains are in triple helical conformation.

(198)

The thermal stabilities ( T ( m ) ) of the COL1, COL2, and COL3 domains were 38, 49 and 40 degrees C, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(199)

The T ( m ) of the central collagenous domain is unusually high, which in the light of this domain being invariant in terms of alternative splicing suggests that the central portion of the molecule may have an important role in the stability of the molecule.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(200)

All in all, most of the type XIII collagen ectodomain appears to be present in triple helical conformation, which is in clear contrast to the short or highly interrupted triple helical domains of the other known collagenous transmembrane proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9342	All in all, most of the Proteintype XIII collagen ectodomain appears to be present in triple helical conformation, which is in clear contrast to the short or highly interrupted triple helical domains of the other known collagenous transmembrane proteins.

(201)

Symmetric and asymmetric DNA methylation in the human IGF2 - H19 imprinted region.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9804	Symmetric and asymmetric DNA methylation in the human ProteinIGF2 - H19 imprinted region.
0.8721	Symmetric and asymmetric DNA_methylationDNA methylation in the human IGF2 - H19 imprinted region.
0.7417	Symmetric and asymmetric DNA methylation in the human IGF2 - H19 Entityimprinted region .
0.9222	Symmetric and asymmetric DNA DNA_methylationmethylation in the human IGF2 - H19 imprinted region.
0.6758	Symmetric and asymmetric DNA_methylationDNA methylation in the human IGF2 - H19 imprinted region.
0.6149	Symmetric and asymmetric DNA methylation in the human IGF2 - H19 Entityimprinted region.

(202)

The two contiguous IGF2 ( human insulin - like growth factor II ) and H19 genes are reciprocally imprinted in both human and mouse.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	The two contiguous IGF2 ( human insulin - like growth factor II ) and ProteinH19 genes are reciprocally imprinted in both human and mouse.
0.9986	The two contiguous ProteinIGF2 ( human insulin - like growth factor II ) and H19 genes are reciprocally imprinted in both human and mouse.
0.8282	The two contiguous IGF2 ( human Proteininsulin - like growth factor II ) and H19 genes are reciprocally imprinted in both human and mouse.

(203)

In most tissues, IGF2 is transcribed only from the paternal chromosome while H19 is transcribed only from the maternal allele.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	In most tissues, IGF2 is transcribed only from the paternal chromosome while ProteinH19 is transcribed only from the maternal allele.
0.9996	In most tissues, ProteinIGF2 is transcribed only from the paternal chromosome while H19 is transcribed only from the maternal allele.

(204)

The presence of a differential methylation region ( DMR ) on the two parental alleles at the 5'flanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.

Precision: 42.86

Recall: 100.00

F1: 60.00

Score	Text
0.9881	The presence of a differential methylation region ( DMR ) on the two parental alleles at the 5'flanking region of ProteinH19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.9682	The presence of a differential DNA_methylationmethylation region ( DMR ) on the two parental alleles at the 5'flanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.8126	The presence of a differential methylation region ( DMR ) on the two parental alleles at the Entity5'flanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.8506	The presence of a differential methylation region ( DMR ) on the two parental alleles at the Entity5 ' flanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.8239	The presence of a differential methylation region ( DMR ) on the two parental alleles at the Entity5'flanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.5867	The presence of a differential methylation region ( DMR ) on the two parental alleles at the 5 ' Entityflanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.5195	The presence of a differential methylation region ( DMR ) on the two parental alleles at the 5 ' Entityflanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.

(205)

Using bisulfite genomic sequencing, we have assessed the methylation status of cytosine ( including 154 CpG sites ) in six CpG - rich regions of the human IGF2 - H19 genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9914	Using bisulfite genomic sequencing, we have assessed the methylation status of cytosine ( including 154 CpG sites ) in six CpG - rich regions of the human ProteinIGF2 - H19 genes.
0.9168	Using bisulfite genomic sequencing, we have assessed the methylation status of Entitycytosine ( including 154 CpG sites ) in six CpG - rich regions of the human IGF2 - H19 genes.
0.9051	Using bisulfite genomic sequencing, we have assessed the DNA_methylationmethylation status of cytosine ( including 154 CpG sites ) in six CpG - rich regions of the human IGF2 - H19 genes.

(206)

In a CpG island near promoter P3 of the IGF2 gene, more than 99. 8 % of all cytosines were converted to thymidine by sodium bisulfite mutagenesis, indicating that none of the CpGs was methylated.

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Text
0.9990	In a CpG island near promoter P3 of the ProteinIGF2 gene, more than 99. 8 % of all cytosines were converted to thymidine by sodium bisulfite mutagenesis, indicating that none of the CpGs was methylated.
0.9128	In a CpG island near promoter P3 of the IGF2 gene, more than 99. 8 % of all cytosines were converted to thymidine by sodium bisulfite mutagenesis, indicating that none of the CpGs was DNA_methylationmethylated .
0.6624	In a EntityCpG island near promoter P3 of the IGF2 gene, more than 99. 8 % of all cytosines were converted to thymidine by sodium bisulfite mutagenesis, indicating that none of the CpGs was methylated.
0.8559	In a CpG island near promoter P3 of the IGF2 gene, more than 99. 8 % of all Entitycytosines were converted to thymidine by sodium bisulfite mutagenesis, indicating that none of the CpGs was methylated.
0.5444	In a CpG island near promoter P3 of the IGF2 gene, more than 99. 8 % of all cytosines were converted to thymidine by sodium bisulfite mutagenesis, indicating that none of the EntityCpGs was methylated.

(207)

In the IGF2 exon 8 - 9 region, mosaic methylation of 56 CpG sites was observed in fetal tissues and in adult blood DNA.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9955	In the ProteinIGF2 exon 8 - 9 region, mosaic methylation of 56 CpG sites was observed in fetal tissues and in adult blood DNA.
0.9840	In the IGF2 exon 8 - 9 region, mosaic DNA_methylationmethylation of 56 CpG sites was observed in fetal tissues and in adult blood DNA.
0.6755	In the IGF2 exon 8 - 9 region, mosaic methylation of 56 EntityCpG sites was observed in fetal tissues and in adult blood DNA.
0.5131	In the IGF2 exon 8 - 9 region, mosaic methylation of 56 EntityCpG sites was observed in fetal tissues and in adult blood DNA.

(208)

In contrast to the mosaic methylation of IGF2, the allelic methylation of the human H19 DMR was uniform.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9983	In contrast to the mosaic methylation of ProteinIGF2 , the allelic methylation of the human H19 DMR was uniform.
0.9495	In contrast to the mosaic methylation of IGF2, the allelic DNA_methylationmethylation of the human H19 DMR was uniform.
0.9479	In contrast to the mosaic methylation of IGF2, the allelic methylation of the human ProteinH19 DMR was uniform.
0.9345	In contrast to the mosaic DNA_methylationmethylation of IGF2, the allelic methylation of the human H19 DMR was uniform.
0.8976	In contrast to the mosaic methylation of IGF2, the allelic methylation of the human H19 EntityDMR was uniform.

(209)

In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all 25 CpG sites were completely methylated on only one parental allele.

Precision: 42.86

Recall: 100.00

F1: 60.00

Score	Text
0.9967	In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the ProteinH19 transcription site, all 25 CpG sites were completely methylated on only one parental allele.
0.9885	In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all 25 CpG sites were completely DNA_methylationmethylated on only one parental allele.
0.8887	In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all Entity25 CpG sites were completely methylated on only one parental allele.
0.9163	In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all 25 EntityCpG sites were completely methylated on only one parental allele.
0.5610	In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all Entity25 CpG sites were completely methylated on only one parental allele.
0.5387	In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all 25 EntityCpG sites were completely methylated on only one parental allele.
0.5386	In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all Entity25 CpG sites were completely methylated on only one parental allele.

(210)

Uniform allele - specific methylation was also observed in the CpG island proximal to the H19 promoter ( - 711 to - 290 ) with complete methylation of all 25 CpG sites in one parental allele.

Precision: 66.67

Recall: 80.00

F1: 72.73

Score	Text
0.9920	Uniform allele - specific DNA_methylationmethylation was also observed in the CpG island proximal to the H19 promoter ( - 711 to - 290 ) with complete methylation of all 25 CpG sites in one parental allele.
0.9834	Uniform allele - specific methylation was also observed in the CpG island proximal to the H19 promoter ( - 711 to - 290 ) with complete DNA_methylationmethylation of all 25 CpG sites in one parental allele.
0.9734	Uniform allele - specific methylation was also observed in the CpG island proximal to the ProteinH19 promoter ( - 711 to - 290 ) with complete methylation of all 25 CpG sites in one parental allele.
0.6477	Uniform allele - specific methylation was also observed in the EntityCpG island proximal to the H19 promoter ( - 711 to - 290 ) with complete methylation of all 25 CpG sites in one parental allele.
0.7181	Uniform allele - specific methylation was also observed in the CpG island proximal to the H19 promoter ( - 711 to - 290 ) with complete methylation of all 25 EntityCpG sites in one parental allele.
0.5004	Uniform allele - specific methylation was also observed in the CpG island proximal to the H19 promoter ( - 711 to - 290 ) with DNA_methylationcomplete methylation of all 25 CpG sites in one parental allele.
	Uniform allele - specific methylation was also observed in the CpG island proximal to the H19 promoter ( - 711 to - 290 ) with complete methylation of all Entity25 CpG sites in one parental allele.

(211)

In contrast, the CpG region in the H19 promoter ( - 292 to + 15 ) was mosaically methylated in all tissues.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9850	In contrast, the CpG region in the H19 promoter ( - 292 to + 15 ) was mosaically DNA_methylationmethylated in all tissues.
0.9604	In contrast, the CpG region in the ProteinH19 promoter ( - 292 to + 15 ) was mosaically methylated in all tissues.
0.8898	In contrast, the EntityCpG region in the H19 promoter ( - 292 to + 15 ) was mosaically methylated in all tissues.
0.7647	In contrast, the EntityCpG region in the H19 promoter ( - 292 to + 15 ) was mosaically methylated in all tissues.

(212)

In addition, cytosine was methylated at three CpNpG and GpNpC sites on the top DNA strand and one CpNpG site on the bottom DNA strand from the fetal brain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9719	In addition, cytosine was DNA_methylationmethylated at three CpNpG and GpNpC sites on the top DNA strand and one CpNpG site on the bottom DNA strand from the fetal brain.
0.9107	In addition, cytosine was methylated at three EntityCpNpG and GpNpC sites on the top DNA strand and one CpNpG site on the bottom DNA strand from the fetal brain.
0.9007	In addition, cytosine was methylated at three CpNpG and EntityGpNpC sites on the top DNA strand and one CpNpG site on the bottom DNA strand from the fetal brain.
0.8889	In addition, cytosine was methylated at three CpNpG and GpNpC sites on the top DNA strand and one EntityCpNpG site on the bottom DNA strand from the fetal brain.

(213)

The cytosines at CpG sites were methylated on both DNA strands ( symmetric methylation ) while cytosines at the CpNpG and GpNpC sites were methylated on only one DNA strand ( asymmetric methylation ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9566	The cytosines at CpG sites were methylated on both DNA strands ( symmetric methylation ) while cytosines at the CpNpG and GpNpC sites were DNA_methylationmethylated on only one DNA strand ( asymmetric methylation ).
0.9546	The cytosines at CpG sites were DNA_methylationmethylated on both DNA strands ( symmetric methylation ) while cytosines at the CpNpG and GpNpC sites were methylated on only one DNA strand ( asymmetric methylation ).
0.9444	The cytosines at CpG sites were methylated on both DNA strands ( symmetric methylation ) while Entitycytosines at the CpNpG and GpNpC sites were methylated on only one DNA strand ( asymmetric methylation ).
0.9244	The Entitycytosines at CpG sites were methylated on both DNA strands ( symmetric methylation ) while cytosines at the CpNpG and GpNpC sites were methylated on only one DNA strand ( asymmetric methylation ).

(214)

The asymmetric methylation was associated with tissue - specific disruption of H19 genomic imprinting in fetal brain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9931	The asymmetric methylation was associated with tissue - specific disruption of ProteinH19 genomic imprinting in fetal brain.
0.9505	The asymmetric DNA_methylationmethylation was associated with tissue - specific disruption of H19 genomic imprinting in fetal brain.

(215)

An increase in histone acetylation and IL - 2 antagonizing the immunoinhibitory effect are necessary for augmentation by butyrate of in vitro anti - TNP antibody production.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9982	An increase in histone acetylation and ProteinIL - 2 antagonizing the immunoinhibitory effect are necessary for augmentation by butyrate of in vitro anti - TNP antibody production.
0.9969	An increase in histone Acetylationacetylation and IL - 2 antagonizing the immunoinhibitory effect are necessary for augmentation by butyrate of in vitro anti - TNP antibody production.
0.9952	An increase in Proteinhistone acetylation and IL - 2 antagonizing the immunoinhibitory effect are necessary for augmentation by butyrate of in vitro anti - TNP antibody production.

(216)

We investigated the role of histone acetylation in the promotion of antigen - specific antibody production in murine B cells induced by sodium butyrate ( NaBu ) plus interleukin 2 ( IL - 2 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9977	We investigated the role of Proteinhistone acetylation in the promotion of antigen - specific antibody production in murine B cells induced by sodium butyrate ( NaBu ) plus interleukin 2 ( IL - 2 ).
0.9954	We investigated the role of histone Acetylationacetylation in the promotion of antigen - specific antibody production in murine B cells induced by sodium butyrate ( NaBu ) plus interleukin 2 ( IL - 2 ).
0.9559	We investigated the role of histone acetylation in the promotion of antigen - specific antibody production in murine B cells induced by sodium butyrate ( NaBu ) plus interleukin 2 Protein( IL - 2 ) .
0.8633	We investigated the role of histone acetylation in the promotion of antigen - specific antibody production in murine B cells induced by sodium butyrate ( NaBu ) plus Proteininterleukin 2 ( IL - 2 ).

(217)

NaBu dose dependently increased the acetylation levels of histone H4 at concentrations which effectively enhanced anti - trinitrophenyl ( TNP ) antibody production in the presence of IL - 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9978	NaBu dose dependently increased the acetylation levels of histone H4 at concentrations which effectively enhanced anti - trinitrophenyl ( TNP ) antibody production in the presence of ProteinIL - 2 .
0.9956	NaBu dose dependently increased the Acetylationacetylation levels of histone H4 at concentrations which effectively enhanced anti - trinitrophenyl ( TNP ) antibody production in the presence of IL - 2.
0.9652	NaBu dose dependently increased the acetylation levels of Proteinhistone H4 at concentrations which effectively enhanced anti - trinitrophenyl ( TNP ) antibody production in the presence of IL - 2.

(218)

Among other short - chain fatty acids and NaBu analogs, propionate, valerate and vinylacetate were effective in the presence of IL - 2 in increasing both antibody production and the histone H4 acetylation level, but acetate, alpha -, beta - and gamma - hydroxybutyrates and alpha -, beta - and gamma - aminobutyrates were not effective, even in the presence of IL - 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9955	Among other short - chain fatty acids and NaBu analogs, propionate, valerate and vinylacetate were effective in the presence of ProteinIL - 2 in increasing both antibody production and the histone H4 acetylation level, but acetate, alpha -, beta - and gamma - hydroxybutyrates and alpha -, beta - and gamma - aminobutyrates were not effective, even in the presence of IL - 2.
0.9955	Among other short - chain fatty acids and NaBu analogs, propionate, valerate and vinylacetate were effective in the presence of IL - 2 in increasing both antibody production and the histone H4 acetylation level, but acetate, alpha -, beta - and gamma - hydroxybutyrates and alpha -, beta - and gamma - aminobutyrates were not effective, even in the presence of ProteinIL - 2 .
0.9899	Among other short - chain fatty acids and NaBu analogs, propionate, valerate and vinylacetate were effective in the presence of IL - 2 in increasing both antibody production and the histone H4 Acetylationacetylation level, but acetate, alpha -, beta - and gamma - hydroxybutyrates and alpha -, beta - and gamma - aminobutyrates were not effective, even in the presence of IL - 2.
0.9753	Among other short - chain fatty acids and NaBu analogs, propionate, valerate and vinylacetate were effective in the presence of IL - 2 in increasing both antibody production and the Proteinhistone H4 acetylation level, but acetate, alpha -, beta - and gamma - hydroxybutyrates and alpha -, beta - and gamma - aminobutyrates were not effective, even in the presence of IL - 2.

(219)

The effect of the specific histone deacetylase inhibitor trichostatin A ( TSA ), which enhances anti - TNP antibody production without IL - 2, was markedly inhibited by adding NaBu simultaneously.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	The effect of the specific histone deacetylase inhibitor trichostatin A ( TSA ), which enhances anti - TNP antibody production without ProteinIL - 2 , was markedly inhibited by adding NaBu simultaneously.
0.9979	The effect of the specific Proteinhistone deacetylase inhibitor trichostatin A ( TSA ), which enhances anti - TNP antibody production without IL - 2, was markedly inhibited by adding NaBu simultaneously.

(220)

However, the effect of TSA was neither inhibited nor potentiated by NaBu in the presence of IL - 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9977	However, the effect of TSA was neither inhibited nor potentiated by NaBu in the presence of ProteinIL - 2 .

(221)

Splenic B cells treated with NaBu, TSA and both together in the presence or absence of IL - 2 showed almost the same increased acetylation level of histone H4.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9962	Splenic B cells treated with NaBu, TSA and both together in the presence or absence of IL - 2 showed almost the same increased Acetylationacetylation level of histone H4.
0.9855	Splenic B cells treated with NaBu, TSA and both together in the presence or absence of ProteinIL - 2 showed almost the same increased acetylation level of histone H4.
0.9659	Splenic B cells treated with NaBu, TSA and both together in the presence or absence of IL - 2 showed almost the same increased acetylation level of Proteinhistone H4 .

(222)

These results suggest that the NaBu - induced enhancement of anti - TNP antibody production in the presence of IL - 2 is mediated through a moderate increase in the level of histone acetylation and that NaBu has both stimulating and inhibiting activities for anti - TNP antibody production, the latter of which is overcome by IL - 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	These results suggest that the NaBu - induced enhancement of anti - TNP antibody production in the presence of IL - 2 is mediated through a moderate increase in the level of histone acetylation and that NaBu has both stimulating and inhibiting activities for anti - TNP antibody production, the latter of which is overcome by ProteinIL - 2 .
0.9987	These results suggest that the NaBu - induced enhancement of anti - TNP antibody production in the presence of ProteinIL - 2 is mediated through a moderate increase in the level of histone acetylation and that NaBu has both stimulating and inhibiting activities for anti - TNP antibody production, the latter of which is overcome by IL - 2.
0.9973	These results suggest that the NaBu - induced enhancement of anti - TNP antibody production in the presence of IL - 2 is mediated through a moderate increase in the level of histone Acetylationacetylation and that NaBu has both stimulating and inhibiting activities for anti - TNP antibody production, the latter of which is overcome by IL - 2.
0.9956	These results suggest that the NaBu - induced enhancement of anti - TNP antibody production in the presence of IL - 2 is mediated through a moderate increase in the level of Proteinhistone acetylation and that NaBu has both stimulating and inhibiting activities for anti - TNP antibody production, the latter of which is overcome by IL - 2.

(223)

New immunofluorescence assays for detection of Human herpesvirus 8 - specific antibodies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(224)

Several assays have been developed for detection of immunoglobulin G antibodies to Human herpesvirus 8 ( HHV - 8 ), including immunofluorescence assays ( IFAs ) and enzyme - linked immunosorbent assays ( ELISAs ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(225)

However, the specificity and sensitivity of these assays are not completely defined due to the lack of a " gold standard. " Although IFAs based on primary effusion lymphoma ( PEL ) cell lines are used widely, the assays can be confounded by nonspecific reactions against cellular components and potential cross - reaction with antibodies against other herpesviruses.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(226)

To provide more reliable IFAs, we established recombinant Semliki Forest viruses ( rSFVs ) expressing the HHV - 8 - specific proteins ORF73 and K8. 1 and used BHK - 21 cells infected with these rSFVs for IFA ( ORF73 - IFA and K8. 1 - IFA ).

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9952	To provide more reliable IFAs, we established recombinant Semliki Forest viruses ( rSFVs ) expressing the HHV - 8 - specific proteins ProteinORF73 and K8. 1 and used BHK - 21 cells infected with these rSFVs for IFA ( ORF73 - IFA and K8. 1 - IFA ).
0.9944	To provide more reliable IFAs, we established recombinant Semliki Forest viruses ( rSFVs ) expressing the HHV - 8 - specific proteins ORF73 and K8. 1 and used BHK - 21 cells infected with these rSFVs for IFA Protein( ORF73 - IFA and K8. 1 - IFA ).
0.9680	To provide more reliable IFAs, we established recombinant Semliki Forest viruses ( rSFVs ) expressing the HHV - 8 - specific proteins ORF73 and ProteinK8. 1 and used BHK - 21 cells infected with these rSFVs for IFA ( ORF73 - IFA and K8. 1 - IFA ).
0.8660	To provide more reliable IFAs, we established recombinant Semliki Forest viruses ( rSFVs ) expressing the HHV - 8 - specific proteins ORF73 and K8. 1 and used BHK - 21 cells infected with these rSFVs for IFA ( ORF73 - IFA and ProteinK8. 1 - IFA ) .
0.8664	To provide more reliable IFAs, we established recombinant Semliki Forest viruses ( rSFVs ) expressing the HHV - 8 - specific proteins ORF73 and K8. 1 and used BHK - 21 cells infected with these rSFVs for IFA ( ORF73 - IFA and K8 Protein. 1 - IFA ) .
0.8454	To provide more reliable IFAs, we established recombinant Semliki Forest viruses ( rSFVs ) expressing the HHV - 8 - specific proteins ORF73 and K8 Protein. 1 and used BHK - 21 cells infected with these rSFVs for IFA ( ORF73 - IFA and K8. 1 - IFA ).

(227)

Expression of the HHV - 8 - specific proteins at very high levels by the rSFV system allowed easy scoring for IFA and thereby increased specificity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(228)

The rSFV system also allowed detection of antibodies against glycosylation - dependent epitopes of K8. 1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.8599	The rSFV system also allowed detection of antibodies against glycosylation - dependent epitopes of ProteinK8. 1 .

(229)

Titers measured by rSFV - based IFAs and PEL - based IFAs correlated well ( correlation coefficients of > 0. 9 ), and concordances of seroreactivities between rSFV - based and PEL - based IFAs were > 97 % ( kappa > 0. 93 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(230)

K8. 1 - IFA was more sensitive than either ORF73 - IFA or peptide ELISAs.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	K8. 1 - IFA was more sensitive than either ProteinORF73 - IFA or peptide ELISAs.

(231)

Using PEL - based lytic IFA as a reference assay, the sensitivity and specificity of K8. 1 - IFA were estimated to be 94 and 100 %, respectively.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9467	Using PEL - based lytic IFA as a reference assay, the sensitivity and specificity of ProteinK8. 1 - IFA were estimated to be 94 and 100 %, respectively.
0.8804	Using PEL - based lytic IFA as a reference assay, the sensitivity and specificity of K8 Protein. 1 - IFA were estimated to be 94 and 100 %, respectively.

(232)

HHV - 8 prevalences determined by K8. 1 - IFA among the human immunodeficiency virus ( HIV ) - positive ( HIV ( + ) ) Kaposi's sarcoma ( KS ) patients, HIV ( + ) KS ( - ) patients, and healthy controls were 100, 65, and 6. 7 %, respectively, which were consistent with prior reports.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9696	HHV - 8 prevalences determined by ProteinK8. 1 - IFA among the human immunodeficiency virus ( HIV ) - positive ( HIV ( + ) ) Kaposi's sarcoma ( KS ) patients, HIV ( + ) KS ( - ) patients, and healthy controls were 100, 65, and 6. 7 %, respectively, which were consistent with prior reports.
0.9475	HHV - 8 prevalences determined by K8 Protein. 1 - IFA among the human immunodeficiency virus ( HIV ) - positive ( HIV ( + ) ) Kaposi's sarcoma ( KS ) patients, HIV ( + ) KS ( - ) patients, and healthy controls were 100, 65, and 6. 7 %, respectively, which were consistent with prior reports.

(233)

Therefore, our rSFV - based IFAs may provide a specific and sensitive method for use in epidemiology studies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(234)

In addition, they will provide a basis for further development of diagnostic tests for HHV - 8 infection.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(235)

Evidence that the lizard helospectin peptides are O - glycosylated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9937	Evidence that the lizard Proteinhelospectin peptides are O - glycosylated.
0.9909	Evidence that the lizard helospectin peptides are GlycosylationO - glycosylated .

(236)

Six forms of helospectin ( a vasoactive intestinal peptide analogue ) were purified from the venom of the Heloderma horridum lizard.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9973	Six forms of Proteinhelospectin ( a vasoactive intestinal peptide analogue ) were purified from the venom of the Heloderma horridum lizard.

(237)

Their identification was performed by combining sequencing by automated Edman degradation and electrospray mass spectrometry analysis on the complete peptides and their tryptic fragments.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(238)

The products resulting from the action of an O - glycosidase were also analysed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(239)

Two forms were identified as the previously named Hs1 and Hs2 of 38 and 37 amino - acid residues, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	Two forms were identified as the previously named ProteinHs1 and Hs2 of 38 and 37 amino - acid residues, respectively.
0.9988	Two forms were identified as the previously named Hs1 and ProteinHs2 of 38 and 37 amino - acid residues, respectively.

(240)

Two forms corresponded to Hs1 and Hs2 O - glycosylated by a N - acetylhexosamine - hexose motif attached to the Ser32 residue.

Precision: 83.33

Recall: 100.00

F1: 90.91

Score	Text
0.9626	Two forms corresponded to Hs1 and Hs2 O - glycosylated by a N - acetylhexosamine - hexose motif attached to the EntitySer32 residue.
0.9589	Two forms corresponded to ProteinHs1 and Hs2 O - glycosylated by a N - acetylhexosamine - hexose motif attached to the Ser32 residue.
0.9497	Two forms corresponded to Hs1 and Hs2 O - glycosylated by a EntityN - acetylhexosamine - hexose motif attached to the Ser32 residue.
0.9493	Two forms corresponded to Hs1 and Hs2 GlycosylationO - glycosylated by a N - acetylhexosamine - hexose motif attached to the Ser32 residue.
0.9234	Two forms corresponded to Hs1 and ProteinHs2 O - glycosylated by a N - acetylhexosamine - hexose motif attached to the Ser32 residue.
0.6560	Two forms corresponded to Hs1 and GlycosylationHs2 O - glycosylated by a N - acetylhexosamine - hexose motif attached to the Ser32 residue.

(241)

Two other forms were not completely characterized but might correspond to the O - glycosylated forms bearing a phosphate or a sulfate group.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.8040	Two other forms were not completely characterized but might correspond to the GlycosylationO - glycosylated forms bearing a phosphate or a sulfate group.

(242)

The glycosylation did not affect the capacity of the helospectins to recognize and to activate the human and the rat VPAC1 and VPAC2 receptors.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9992	The glycosylation did not affect the capacity of the helospectins to recognize and to activate the human and the rat ProteinVPAC1 and VPAC2 receptors.
0.9992	The glycosylation did not affect the capacity of the helospectins to recognize and to activate the human and the rat VPAC1 and ProteinVPAC2 receptors.
0.9919	The glycosylation did not affect the capacity of the Proteinhelospectins to recognize and to activate the human and the rat VPAC1 and VPAC2 receptors.
0.9814	The Glycosylationglycosylation did not affect the capacity of the helospectins to recognize and to activate the human and the rat VPAC1 and VPAC2 receptors.

(243)

Changes in methionine adenosyltransferase and S - adenosylmethionine homeostasis in alcoholic rat liver.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(244)

Liver - specific and non - liver - specific methionine adenosyltransferase ( MAT ) are products of two genes, MAT1A and MAT2A, respectively, that catalyze the formation of S - adenosylmethionine ( SAM ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9985	Liver - specific and non - liver - specific methionine adenosyltransferase ( MAT ) are products of two genes, MAT1A and ProteinMAT2A , respectively, that catalyze the formation of S - adenosylmethionine ( SAM ).
0.9983	Liver - specific and non - liver - specific methionine adenosyltransferase ( MAT ) are products of two genes, ProteinMAT1A and MAT2A, respectively, that catalyze the formation of S - adenosylmethionine ( SAM ).

(245)

We previously showed that MAT2A expression was associated with more rapid cell growth.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9971	We previously showed that ProteinMAT2A expression was associated with more rapid cell growth.

(246)

Changes in MAT expression have not been examined in animal models of alcoholic liver injury, which is the focus of the current study.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(247)

After rats were fed intragastrically with ethanol and high fat for 9 wk, the mRNA level of both MAT forms doubled but only the protein level of MAT2A increased.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9981	After rats were fed intragastrically with ethanol and high fat for 9 wk, the mRNA level of both MAT forms doubled but only the protein level of ProteinMAT2A increased.

(248)

Although liver - specific MAT activity did not change, it was 32 % lower after one and 68 % lower after eight weekly enteral doses of lipopolysaccharide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(249)

Hepatic levels of methionine, SAM, and DNA methylation fell by approximately 40 %. c - myc was hypomethylated, and its mRNA level increased.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Hepatic levels of methionine, SAM, and DNA methylation fell by approximately 40 %. Proteinc - myc was hypomethylated, and its mRNA level increased.
0.9195	Hepatic levels of methionine, SAM, and DNA methylation fell by approximately 40 %. c - myc was DNA_methylationhypomethylated , and its mRNA level increased.

(250)

Genome - wide DNA strand break increased.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(251)

Thus in the prefibrotic stage of alcoholic liver injury, there is already a switch in MAT expression, global DNA hypomethylation, increased c - myc expression, and genome - wide DNA strand break.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Thus in the prefibrotic stage of alcoholic liver injury, there is already a switch in MAT expression, global DNA hypomethylation, increased Proteinc - myc expression, and genome - wide DNA strand break.

(252)

These changes may be important in predisposing this liver disease to malignant degeneration.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(253)

Hsl7p, the yeast homologue of human JBP1, is a protein methyltransferase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	Hsl7p, the yeast homologue of human ProteinJBP1 , is a protein methyltransferase.
0.9994	ProteinHsl7p , the yeast homologue of human JBP1, is a protein methyltransferase.

(254)

The yeast protein Hsl7p is a homologue of Janus kinase binding protein 1, JBP1, a newly characterized protein methyltransferase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	The yeast protein ProteinHsl7p is a homologue of Janus kinase binding protein 1, JBP1, a newly characterized protein methyltransferase.
0.9993	The yeast protein Hsl7p is a homologue of Janus kinase binding protein 1, ProteinJBP1 , a newly characterized protein methyltransferase.
0.7987	The yeast protein Hsl7p is a homologue of ProteinJanus kinase binding protein 1 , JBP1, a newly characterized protein methyltransferase.

(255)

In this report, Hsl7p also is shown to be a methyltransferase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	In this report, ProteinHsl7p also is shown to be a methyltransferase.

(256)

It can be crosslinked to [ ( 3 ) H ] S - adenosylmethionine and exhibits in vitro protein methylation activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(257)

Calf histones H2A and H4 and bovine myelin basic protein were methylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.

Precision: 100.00

Recall: 90.00

F1: 94.74

Score	Text
0.9970	Calf histones H2A and H4 and bovine myelin basic protein were methylated by ProteinHsl7p , whereas histones H1, H2B, and H3 and bovine cytochrome c were not.
0.9893	Calf histones H2A and ProteinH4 and bovine myelin basic protein were methylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.
0.9845	Calf histones H2A and H4 and bovine myelin basic protein were methylated by Hsl7p, whereas histones H1, ProteinH2B , and H3 and bovine cytochrome c were not.
0.9780	Calf histones H2A and H4 and bovine myelin basic protein were methylated by Hsl7p, whereas histones H1, H2B, and ProteinH3 and bovine cytochrome c were not.
0.9716	Calf histones H2A and H4 and bovine myelin basic protein were methylated by Hsl7p, whereas Proteinhistones H1 , H2B, and H3 and bovine cytochrome c were not.
0.9619	Calf Proteinhistones H2A and H4 and bovine myelin basic protein were methylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.
0.9281	Calf histones H2A and H4 and bovine myelin basic protein were methylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine Proteincytochrome c were not.
0.9184	Calf histones H2A and H4 and bovine Proteinmyelin basic protein were methylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.
0.6789	Calf histones H2A and H4 and bovine myelin basic protein were Catalysismethylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.
	Calf histones H2A and H4 and bovine myelin basic protein were Methylationmethylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.

(258)

We demonstrated that JBP1 can complement Saccharomyces cerevisiae with a disrupted HSL7 gene as judged by a reduction of the elongated bud phenotype, and a point mutation in the JBP1 S - adenosylmethionine consensus binding sequence eliminated all complementation by JBP1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	We demonstrated that JBP1 can complement Saccharomyces cerevisiae with a disrupted HSL7 gene as judged by a reduction of the elongated bud phenotype, and a point mutation in the JBP1 S - adenosylmethionine consensus binding sequence eliminated all complementation by ProteinJBP1 .
0.9999	We demonstrated that ProteinJBP1 can complement Saccharomyces cerevisiae with a disrupted HSL7 gene as judged by a reduction of the elongated bud phenotype, and a point mutation in the JBP1 S - adenosylmethionine consensus binding sequence eliminated all complementation by JBP1.
0.9997	We demonstrated that JBP1 can complement Saccharomyces cerevisiae with a disrupted ProteinHSL7 gene as judged by a reduction of the elongated bud phenotype, and a point mutation in the JBP1 S - adenosylmethionine consensus binding sequence eliminated all complementation by JBP1.
0.9994	We demonstrated that JBP1 can complement Saccharomyces cerevisiae with a disrupted HSL7 gene as judged by a reduction of the elongated bud phenotype, and a point mutation in the ProteinJBP1 S - adenosylmethionine consensus binding sequence eliminated all complementation by JBP1.

(259)

Therefore, we conclude the yeast protein Hsl7p is a sequence and functional homologue of JBP1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Therefore, we conclude the yeast protein Hsl7p is a sequence and functional homologue of ProteinJBP1 .
0.9998	Therefore, we conclude the yeast protein ProteinHsl7p is a sequence and functional homologue of JBP1.

(260)

These data provide evidence for an intricate link between protein methylation and macroscopic changes in yeast morphology.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(261)

Rapid induction of histone hyperacetylation and cellular differentiation in human breast tumor cell lines following degradation of histone deacetylase - 1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9962	Rapid induction of Proteinhistone hyperacetylation and cellular differentiation in human breast tumor cell lines following degradation of histone deacetylase - 1.
0.9450	Rapid induction of histone Acetylationhyperacetylation and cellular differentiation in human breast tumor cell lines following degradation of histone deacetylase - 1.
0.6429	Rapid induction of histone hyperacetylation and cellular differentiation in human breast tumor cell lines following degradation of Proteinhistone deacetylase - 1 .

(262)

Quinidine inhibits proliferation and promotes cellular differentiation in human breast tumor epithelial cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(263)

Previously we showed quinidine arrested MCF - 7 cells in G ( 1 ) phase of the cell cycle and led to a G ( 1 ) to G ( 0 ) transition followed by apoptotic cell death.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(264)

The present experiments demonstrated that MCF - 7, MCF - 7ras, T47D, MDA - MB - 231, and MDA - MB - 435 cells transiently differentiate before undergoing apoptosis in response to quinidine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(265)

The cells accumulated lipid droplets, and the cytokeratin 18 cytoskeleton was reorganized.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9385	The cells accumulated lipid droplets, and the Proteincytokeratin 18 cytoskeleton was reorganized.

(266)

Hyperacetylated histone H4 appeared within 2 h of the addition of quinidine to the medium, and levels were maximal by 24 h.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9712	AcetylationHyperacetylated histone H4 appeared within 2 h of the addition of quinidine to the medium, and levels were maximal by 24 h.
0.9591	Hyperacetylated Proteinhistone H4 appeared within 2 h of the addition of quinidine to the medium, and levels were maximal by 24 h.

(267)

Quinidine - treated MCF - 7 cells showed elevated p21 ( WAF1 ), hypophosphorylation and suppression of retinoblastoma protein, and down - regulation of cyclin D1, similar to the cell cycle response observed with cells induced to differentiate by histone deacetylase inhibitors, trichostatin A, and trapoxin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9877	Quinidine - treated MCF - 7 cells showed elevated Proteinp21 ( WAF1 ) , hypophosphorylation and suppression of retinoblastoma protein, and down - regulation of cyclin D1, similar to the cell cycle response observed with cells induced to differentiate by histone deacetylase inhibitors, trichostatin A, and trapoxin.
0.9867	Quinidine - treated MCF - 7 cells showed elevated p21 ( WAF1 ), hypophosphorylation and suppression of retinoblastoma protein, and down - regulation of cyclin D1, similar to the cell cycle response observed with cells induced to differentiate by Proteinhistone deacetylase inhibitors, trichostatin A, and trapoxin.
0.9713	Quinidine - treated MCF - 7 cells showed elevated p21 ( WAF1 ), hypophosphorylation and suppression of Proteinretinoblastoma protein, and down - regulation of cyclin D1, similar to the cell cycle response observed with cells induced to differentiate by histone deacetylase inhibitors, trichostatin A, and trapoxin.
0.9423	Quinidine - treated MCF - 7 cells showed elevated p21 ( WAF1 ), hypophosphorylation and suppression of retinoblastoma protein, and down - regulation of Proteincyclin D1 , similar to the cell cycle response observed with cells induced to differentiate by histone deacetylase inhibitors, trichostatin A, and trapoxin.
0.9187	Quinidine - treated MCF - 7 cells showed elevated p21 ( WAF1 ), Phosphorylationhypophosphorylation and suppression of retinoblastoma protein, and down - regulation of cyclin D1, similar to the cell cycle response observed with cells induced to differentiate by histone deacetylase inhibitors, trichostatin A, and trapoxin.

(268)

Quinidine did not show evidence for direct inhibition of histone deacetylase enzymatic activity in vitro.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	Quinidine did not show evidence for direct inhibition of Proteinhistone deacetylase enzymatic activity in vitro.

(269)

HDAC1 was undetectable in MCF - 7 cells 30 min after addition of quinidine to the growth medium.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	ProteinHDAC1 was undetectable in MCF - 7 cells 30 min after addition of quinidine to the growth medium.

(270)

The proteasome inhibitors MG - 132 and lactacystin completely protected HDAC1 from the action of quinidine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	The proteasome inhibitors MG - 132 and lactacystin completely protected ProteinHDAC1 from the action of quinidine.

(271)

We conclude that quinidine is a breast tumor cell differentiating agent that causes the loss of HDAC1 via a proteasomal sensitive mechanism.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	We conclude that quinidine is a breast tumor cell differentiating agent that causes the loss of ProteinHDAC1 via a proteasomal sensitive mechanism.

(272)

Glycosylation influences gating and pH sensitivity of I ( sK ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(273)

The KvLQT1 and minK subunits that coassemble to form I ( sK ) channels, contain potential N - glycosylation sites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	The ProteinKvLQT1 and minK subunits that coassemble to form I ( sK ) channels, contain potential N - glycosylation sites.
0.9992	The KvLQT1 and ProteinminK subunits that coassemble to form I ( sK ) channels, contain potential N - glycosylation sites.

(274)

To examine the role of glycosylation in channel function, a Chinese hamster ovary cell line deficient in glycosylation ( Lec - 1 ) and its parental cell line ( Pro - 5 ) were transiently transfected with human KvLQT1 ( hKvLQT1 ) cDNA, alone and in combination with the rat ( rminK ) or human minK ( hminK ) cDNA.

Precision: 62.50

Recall: 100.00

F1: 76.92

Score	Text
0.9930	To examine the role of glycosylation in channel function, a Chinese hamster ovary cell line deficient in glycosylation ( Lec - 1 ) and its parental cell line ( Pro - 5 ) were transiently transfected with human KvLQT1 ( hKvLQT1 ) cDNA, alone and in combination with the rat Protein( rminK ) or human minK ( hminK ) cDNA.
0.9878	To examine the role of glycosylation in channel function, a Chinese hamster ovary cell line deficient in glycosylation ( Lec - 1 ) and its parental cell line ( Pro - 5 ) were transiently transfected with human ProteinKvLQT1 ( hKvLQT1 ) cDNA, alone and in combination with the rat ( rminK ) or human minK ( hminK ) cDNA.
0.9802	To examine the role of glycosylation in channel function, a Chinese hamster ovary cell line deficient in glycosylation ( Lec - 1 ) and its parental cell line ( Pro - 5 ) were transiently transfected with human KvLQT1 ( hKvLQT1 ) cDNA, alone and in combination with the rat ( rminK ) or human ProteinminK ( hminK ) cDNA.
0.9755	To examine the role of glycosylation in channel function, a Chinese hamster ovary cell line deficient in glycosylation ( Lec - 1 ) and its parental cell line ( Pro - 5 ) were transiently transfected with human KvLQT1 ( hKvLQT1 ) cDNA, alone and in combination with the rat ( rminK ) or human minK Protein( hminK ) cDNA.
0.9669	To examine the role of glycosylation in channel function, a Chinese hamster ovary cell line deficient in glycosylation ( Lec - 1 ) and its parental cell line ( Pro - 5 ) were transiently transfected with human KvLQT1 Protein( hKvLQT1 ) cDNA, alone and in combination with the rat ( rminK ) or human minK ( hminK ) cDNA.
0.9427	To examine the role of glycosylation in channel function, a Chinese hamster ovary cell line deficient in glycosylation ( Lec - 1 ) and its parental cell line ( Pro - 5 ) were transiently transfected with human KvLQT1 ( hKvLQT1 ) cDNA, alone and in combination with the rat ( rminK ) or human ProteinminK ( hminK ) cDNA.
0.9248	To examine the role of glycosylation in channel function, a Chinese hamster ovary cell line deficient in glycosylation ( Lec - 1 ) and its parental cell line ( Pro - 5 ) were transiently transfected with human ProteinKvLQT1 ( hKvLQT1 ) cDNA, alone and in combination with the rat ( rminK ) or human minK ( hminK ) cDNA.
0.6275	To examine the role of glycosylation in channel function, a Chinese hamster ovary cell line deficient in Glycosylationglycosylation ( Lec - 1 ) and its parental cell line ( Pro - 5 ) were transiently transfected with human KvLQT1 ( hKvLQT1 ) cDNA, alone and in combination with the rat ( rminK ) or human minK ( hminK ) cDNA.

(275)

Functional KvLQT1 and I ( sK ) currents were expressed in both cell lines, although amplitudes were larger in Pro - 5 than Lec - 1 cells transfected with hKvLQT1 and hKvLQT1 / hminK.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Functional KvLQT1 and I ( sK ) currents were expressed in both cell lines, although amplitudes were larger in Pro - 5 than Lec - 1 cells transfected with ProteinhKvLQT1 and hKvLQT1 / hminK.
0.9995	Functional KvLQT1 and I ( sK ) currents were expressed in both cell lines, although amplitudes were larger in Pro - 5 than Lec - 1 cells transfected with hKvLQT1 and ProteinhKvLQT1 / hminK .
0.9994	Functional ProteinKvLQT1 and I ( sK ) currents were expressed in both cell lines, although amplitudes were larger in Pro - 5 than Lec - 1 cells transfected with hKvLQT1 and hKvLQT1 / hminK.

(276)

For I ( sK ), but not KvLQT1, the voltage - dependence of activation was shifted to more positive voltages and the activation kinetics were slower in the Lec - 1 compared to the Pro - 5 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	For I ( sK ), but not ProteinKvLQT1 , the voltage - dependence of activation was shifted to more positive voltages and the activation kinetics were slower in the Lec - 1 compared to the Pro - 5 cells.

(277)

The effect of extracellular acidification on recombinant KvLQT1 and I ( sK ) currents was investigated in Pro - 5 and Lec - 1 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	The effect of extracellular acidification on recombinant ProteinKvLQT1 and I ( sK ) currents was investigated in Pro - 5 and Lec - 1 cells.

(278)

Changing external pH ( pH ( o ) ) from 7. 4 to 6. 0 significantly decreased the amplitude and increased the half - activation voltage ( V ( 1 / 2 ) ) of KvLQT1 currents in Pro - 5 and Lec - 1 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Changing external pH ( pH ( o ) ) from 7. 4 to 6. 0 significantly decreased the amplitude and increased the half - activation voltage ( V ( 1 / 2 ) ) of ProteinKvLQT1 currents in Pro - 5 and Lec - 1 cells.

(279)

In Pro - 5 cells, decreasing pH ( o ) reduced I ( sK ) amplitude without increasing V ( 1 / 2 ), whether rminK or hminK was coexpressed with hKvLQT.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	In Pro - 5 cells, decreasing pH ( o ) reduced I ( sK ) amplitude without increasing V ( 1 / 2 ), whether rminK or ProteinhminK was coexpressed with hKvLQT.
0.9997	In Pro - 5 cells, decreasing pH ( o ) reduced I ( sK ) amplitude without increasing V ( 1 / 2 ), whether ProteinrminK or hminK was coexpressed with hKvLQT.
0.9997	In Pro - 5 cells, decreasing pH ( o ) reduced I ( sK ) amplitude without increasing V ( 1 / 2 ), whether rminK or hminK was coexpressed with ProteinhKvLQT .

(280)

In contrast, changing pH ( o ) from 7. 4 to 6. 0 did not significantly change I ( sK ) amplitude in Lec - 1 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(281)

Thus, oligosaccharides attached to the minK subunit affect not only the gating properties, but also the pH sensitivity of I ( sK ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9940	Thus, oligosaccharides attached to the ProteinminK subunit affect not only the gating properties, but also the pH sensitivity of I ( sK ).
0.9781	Thus, Entityoligosaccharides attached to the minK subunit affect not only the gating properties, but also the pH sensitivity of I ( sK ).
0.8683	Thus, oligosaccharides Glycosylationattached to the minK subunit affect not only the gating properties, but also the pH sensitivity of I ( sK ).

(282)

A novel post - translational modification of yeast elongation factor 1A. Methylesterification at the C terminus.

Precision: 40.00

Recall: 66.67

F1: 50.00

Score	Text
0.9461	A novel post - translational modification of yeast Proteinelongation factor 1A . Methylesterification at the C terminus.
0.6711	A novel post - translational modification of yeast elongation factor 1A. Methylesterification at the C Entityterminus.
0.7514	A novel post - translational modification of yeast elongation factor 1A. Methylesterification at the C Entityterminus .
0.6105	A novel post - translational modification of yeast elongation factor 1A. Methylesterification at the C terminus Entity.
0.5136	A novel post - translational modification of yeast elongation factor 1A. MethylationMethylesterification at the C terminus.
	A novel post - translational modification of yeast elongation factor 1A. Methylesterification Methylationat the C terminus.

(283)

Protein methylation reactions can play important roles in cell physiology.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(284)

After labeling intact Saccharomyces cerevisiae cells with S - adenosyl - l - [ methyl - ( 3 ) H ] methionine, we identified a major methylated 49 - kDa polypeptide containing [ ( 3 ) H ] methyl groups in two distinct types of linkages.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(285)

Peptide sequence analysis of the purified methylated protein revealed that it is eukaryotic elongation factor 1A ( eEF1A, formerly EF - 1alpha ), the protein that forms a complex with GTP and aminoacyl - tRNAs for binding to the ribosomal A site during protein translation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9972	Peptide sequence analysis of the purified methylated protein revealed that it is eukaryotic elongation factor 1A ( eEF1A, formerly ProteinEF - 1alpha ) , the protein that forms a complex with GTP and aminoacyl - tRNAs for binding to the ribosomal A site during protein translation.
0.9606	Peptide sequence analysis of the purified methylated protein revealed that it is eukaryotic elongation factor 1A Protein( eEF1A , formerly EF - 1alpha ), the protein that forms a complex with GTP and aminoacyl - tRNAs for binding to the ribosomal A site during protein translation.
0.8489	Peptide sequence analysis of the purified methylated protein revealed that it is eukaryotic Proteinelongation factor 1A ( eEF1A, formerly EF - 1alpha ), the protein that forms a complex with GTP and aminoacyl - tRNAs for binding to the ribosomal A site during protein translation.

(286)

Previous studies have shown that eEF1A is methylated on several internal lysine residues to give mono -, di -, and tri - N - epsilon - methyl - lysine derivatives.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9971	Previous studies have shown that ProteineEF1A is methylated on several internal lysine residues to give mono -, di -, and tri - N - epsilon - methyl - lysine derivatives.
0.9474	Previous studies have shown that eEF1A is Methylationmethylated on several internal lysine residues to give mono -, di -, and tri - N - epsilon - methyl - lysine derivatives.
0.9245	Previous studies have shown that eEF1A is methylated on several internal Entitylysine residues to give mono -, di -, and tri - N - epsilon - methyl - lysine derivatives.
0.6905	Previous studies have shown that eEF1A is methylated on several internal Entitylysine residues to give mono -, di -, and tri - N - epsilon - methyl - lysine derivatives.

(287)

We confirm this finding but also detect methylation that is released as volatile methyl groups after base hydrolysis, characteristic of ester linkages.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.5146	We confirm this finding but also detect Methylationmethylation that is released as volatile methyl groups after base hydrolysis, characteristic of ester linkages.

(288)

In cycloheximide - treated cells, methyl esterified eEF1A was detected largely in the ribosome and polysome fractions ; little or no methylated protein was found in the soluble fraction.

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Text
0.9973	In cycloheximide - treated cells, methyl esterified ProteineEF1A was detected largely in the ribosome and polysome fractions ; little or no methylated protein was found in the soluble fraction.
0.9578	In cycloheximide - treated cells, methyl esterified eEF1A was detected largely in the ribosome and polysome fractions ; little or no Methylationmethylated protein was found in the soluble fraction.
0.8917	In cycloheximide - treated cells, Methylationmethyl esterified eEF1A was detected largely in the ribosome and polysome fractions ; little or no methylated protein was found in the soluble fraction.
0.9208	In cycloheximide - treated cells, Methylationmethyl esterified eEF1A was detected largely in the ribosome and polysome fractions ; little or no methylated protein was found in the soluble fraction.
0.9066	In cycloheximide - treated cells, methyl Methylationesterified eEF1A was detected largely in the ribosome and polysome fractions ; little or no methylated protein was found in the soluble fraction.

(289)

Because the base - labile, volatile [ methyl - ( 3 ) H ] radioactivity of eEF1A could be released by trypsin treatment but not by carboxypeptidase Y or chymotrypsin treatment, we suggest that the methyl ester is present on the alpha - carboxyl group of its C - terminal lysine residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9985	Because the base - labile, volatile [ methyl - ( 3 ) H ] radioactivity of ProteineEF1A could be released by trypsin treatment but not by carboxypeptidase Y or chymotrypsin treatment, we suggest that the methyl ester is present on the alpha - carboxyl group of its C - terminal lysine residue.
0.9895	Because the base - labile, volatile [ methyl - ( 3 ) H ] radioactivity of eEF1A could be released by trypsin treatment but not by carboxypeptidase Y or Proteinchymotrypsin treatment, we suggest that the methyl ester is present on the alpha - carboxyl group of its C - terminal lysine residue.
0.9726	Because the base - labile, volatile [ methyl - ( 3 ) H ] radioactivity of eEF1A could be released by trypsin treatment but not by Proteincarboxypeptidase Y or chymotrypsin treatment, we suggest that the methyl ester is present on the alpha - carboxyl group of its C - terminal lysine residue.

(290)

From the results of pulse - chase experiments using radiolabeled intact yeast cells, we find that the N - methylated lysine residues of eEF1A are stable over 4 h, whereas the eEF1A carboxyl methyl ester has a half - life of less than 10 min.

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9822	From the results of pulse - chase experiments using radiolabeled intact yeast cells, we find that the N - methylated lysine residues of ProteineEF1A are stable over 4 h, whereas the eEF1A carboxyl methyl ester has a half - life of less than 10 min.
0.9752	From the results of pulse - chase experiments using radiolabeled intact yeast cells, we find that the N - methylated lysine residues of eEF1A are stable over 4 h, whereas the ProteineEF1A carboxyl methyl ester has a half - life of less than 10 min.
0.9606	From the results of pulse - chase experiments using radiolabeled intact yeast cells, we find that the N - methylated Entitylysine residues of eEF1A are stable over 4 h, whereas the eEF1A carboxyl methyl ester has a half - life of less than 10 min.
0.9305	From the results of pulse - chase experiments using radiolabeled intact yeast cells, we find that the MethylationN - methylated lysine residues of eEF1A are stable over 4 h, whereas the eEF1A carboxyl methyl ester has a half - life of less than 10 min.
0.9633	From the results of pulse - chase experiments using radiolabeled intact yeast cells, we find that the N - methylated Entitylysine residues of eEF1A are stable over 4 h, whereas the eEF1A carboxyl methyl ester has a half - life of less than 10 min.

(291)

The rapid turnover of the methyl ester suggests that the methylation / demethylation of eEF1A at the C - terminal carboxyl group may represent a novel mode of regulation of the activity of this protein in yeast.

Precision: 60.00

Recall: 75.00

F1: 66.67

Score	Text
0.9869	The rapid turnover of the methyl ester suggests that the methylation / demethylation of ProteineEF1A at the C - terminal carboxyl group may represent a novel mode of regulation of the activity of this protein in yeast.
0.8168	The rapid turnover of the methyl ester suggests that the methylation / demethylation of eEF1A at the EntityC - terminal carboxyl group may represent a novel mode of regulation of the activity of this protein in yeast.
0.6890	The rapid turnover of the methyl ester suggests that the Methylationmethylation / demethylation of eEF1A at the C - terminal carboxyl group may represent a novel mode of regulation of the activity of this protein in yeast.
0.6115	The rapid turnover of the methyl ester suggests that the methylation / demethylation of eEF1A at the EntityC - terminal carboxyl group may represent a novel mode of regulation of the activity of this protein in yeast.
0.5162	The rapid turnover of the methyl ester suggests that the methylation / demethylation of eEF1A at the EntityC - terminal carboxyl group may represent a novel mode of regulation of the activity of this protein in yeast.
	The rapid turnover of the methyl ester suggests that the Demethylationmethylation / demethylation of eEF1A at the C - terminal carboxyl group may represent a novel mode of regulation of the activity of this protein in yeast.

(292)

Decreased UDP - GlcNAc levels abrogate proliferation control in EMeg32 - deficient cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Decreased UDP - GlcNAc levels abrogate proliferation control in ProteinEMeg32 - deficient cells.

(293)

The hexosamine pathway provides UDP - N : - acetylhexosamine donor substrates used in cytosolic and Golgi - mediated glycosylation of proteins and for formation of glycosylphosphatidylinositol ( GPI ) anchors, which tether proteins to the outer plasma membrane.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(294)

We have recently identified the murine glucosamine - 6 - phosphate ( GlcN6P ) acetyltransferase, EMeg32, as a developmentally regulated enzyme on the route to UDP - N : - acetylglucosamine ( UDP - GlcNAc ).

Precision: 100.00

Recall: 50.00

F1: 66.67

Score	Text
0.9974	We have recently identified the murine glucosamine - 6 - phosphate ( GlcN6P ) acetyltransferase, ProteinEMeg32 , as a developmentally regulated enzyme on the route to UDP - N : - acetylglucosamine ( UDP - GlcNAc ).
	We have recently identified the murine Proteinglucosamine - 6 - phosphate ( GlcN6P ) acetyltransferase , EMeg32, as a developmentally regulated enzyme on the route to UDP - N : - acetylglucosamine ( UDP - GlcNAc ).

(295)

Here we describe embryos and cells that have the EMeg32 gene inactivated by homologous recombination.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	Here we describe embryos and cells that have the ProteinEMeg32 gene inactivated by homologous recombination.

(296)

Homozygous mutant embryos die at around embryonic day ( E ) 7. 5 with a general proliferative delay of development.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(297)

In vitro differentiated EMeg32 ( - / - ) ES cells show reduced proliferation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9943	In vitro differentiated ProteinEMeg32 ( - / - ) ES cells show reduced proliferation.

(298)

Mouse embryonic fibroblasts ( MEFs ) deficient for EMeg32 exhibit defects in proliferation and adhesiveness, which could be complemented by stable re - expression of EMeg32 or by nutritional restoration of intracellular UDP - GlcNAc levels.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Mouse embryonic fibroblasts ( MEFs ) deficient for EMeg32 exhibit defects in proliferation and adhesiveness, which could be complemented by stable re - expression of ProteinEMeg32 or by nutritional restoration of intracellular UDP - GlcNAc levels.
0.9997	Mouse embryonic fibroblasts ( MEFs ) deficient for ProteinEMeg32 exhibit defects in proliferation and adhesiveness, which could be complemented by stable re - expression of EMeg32 or by nutritional restoration of intracellular UDP - GlcNAc levels.

(299)

Reduced UDP - GlcNAc levels predominantly translated into decreased O - GlcNAc modifications of cytosolic and nuclear proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(300)

Interestingly, growth - impaired EMeg32 ( - / - ) MEFs withstand a number of apoptotic stimuli and express activated PKB / AKT.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	Interestingly, growth - impaired EMeg32 ( - / - ) MEFs withstand a number of apoptotic stimuli and express activated ProteinPKB / AKT .
0.9876	Interestingly, growth - impaired ProteinEMeg32 ( - / - ) MEFs withstand a number of apoptotic stimuli and express activated PKB / AKT.

(301)

Thus, EMeg32 - dependent UDP - GlcNAc levels influence cell cycle progression and susceptibility to apoptotic stimuli.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9572	Thus, ProteinEMeg32 - dependent UDP - GlcNAc levels influence cell cycle progression and susceptibility to apoptotic stimuli.

(302)

Post - translational hydroxylation at the N - terminus of the prion protein reveals presence of PPII structure in vivo.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9851	Post - translational hydroxylation at the EntityN - terminus of the prion protein reveals presence of PPII structure in vivo.
0.9833	Post - translational Hydroxylationhydroxylation at the N - terminus of the prion protein reveals presence of PPII structure in vivo.
0.8842	Post - translational hydroxylation at the N - terminus of the Proteinprion protein reveals presence of PPII structure in vivo.
0.5360	Post - translational hydroxylation at the N - terminus of the Proteinprion protein reveals presence of PPII structure in vivo.

(303)

The transmissible spongiform encephalopathies are characterized by conversion of a host protein, PrP ( C ) ( cellular prion protein ), to a protease - resistant isoform, PrP ( Sc ) ( prion protein scrapie isoform ).

Precision: 75.00

Recall: 75.00

F1: 75.00

Score	Text
0.9991	The transmissible spongiform encephalopathies are characterized by conversion of a host protein, ProteinPrP ( C ) ( cellular prion protein ), to a protease - resistant isoform, PrP ( Sc ) ( prion protein scrapie isoform ).
0.9858	The transmissible spongiform encephalopathies are characterized by conversion of a host protein, PrP ( C ) ( cellular prion protein ), to a protease - resistant isoform, ProteinPrP ( Sc ) ( prion protein scrapie isoform ).
0.9844	The transmissible spongiform encephalopathies are characterized by conversion of a host protein, PrP ( C ) ( cellular Proteinprion protein ) , to a protease - resistant isoform, PrP ( Sc ) ( prion protein scrapie isoform ).
0.8187	The transmissible spongiform encephalopathies are characterized by conversion of a host protein, PrP ( C ) ( cellular prion protein ), to a protease - resistant isoform, ProteinPrP ( Sc ) ( prion protein scrapie isoform ).
	The transmissible spongiform encephalopathies are characterized by conversion of a host protein, PrP ( C ) ( cellular prion protein ), to a protease - resistant isoform, PrP ( Sc ) Protein( prion protein scrapie isoform ).

(304)

The importance of the highly flexible, N - terminal region of PrP has recently become more widely appreciated, particularly the biological activities associated with its metal ion - binding domain and its potential to form a poly ( L - proline ) II ( PPII ) helix.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	The importance of the highly flexible, N - terminal region of ProteinPrP has recently become more widely appreciated, particularly the biological activities associated with its metal ion - binding domain and its potential to form a poly ( L - proline ) II ( PPII ) helix.

(305)

Circular dichroism spectroscopy of an N - terminal peptide, PrP ( 37 - 53 ), showed that the PPII helix is formed in aqueous buffer ; as it also contains an Xaa - Pro - Gly consensus sequence, it may act as a substrate for the collagen - modifying enzyme prolyl 4 - hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9977	Circular dichroism spectroscopy of an N - terminal peptide, ProteinPrP ( 37 - 53 ) , showed that the PPII helix is formed in aqueous buffer ; as it also contains an Xaa - Pro - Gly consensus sequence, it may act as a substrate for the collagen - modifying enzyme prolyl 4 - hydroxylase.

(306)

Direct evidence for this modification was obtained by mass spectrometry and Edman sequencing in recombinant mouse PrP secreted from stably transfected Chinese hamster ovary cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	Direct evidence for this modification was obtained by mass spectrometry and Edman sequencing in recombinant mouse ProteinPrP secreted from stably transfected Chinese hamster ovary cells.

(307)

Almost complete conversion of proline to 4 - hydroxyproline occurs specifically at residue Pro44 of this murine protein ; the same hydroxylated residue was detected, at lower levels, in PrP ( Sc ) from the brains of scrapie - infected mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9822	Almost complete conversion of proline to 4 - hydroxyproline occurs specifically at residue Pro44 of this murine protein ; the same hydroxylated residue was detected, at lower levels, in ProteinPrP ( Sc ) from the brains of scrapie - infected mice.
0.9815	Almost complete conversion of proline to 4 - hydroxyproline occurs specifically at residue Pro44 of this murine protein ; the same Hydroxylationhydroxylated residue was detected, at lower levels, in PrP ( Sc ) from the brains of scrapie - infected mice.
0.9782	Almost complete conversion of proline to 4 - hydroxyproline occurs specifically at residue EntityPro44 of this murine protein ; the same hydroxylated residue was detected, at lower levels, in PrP ( Sc ) from the brains of scrapie - infected mice.
0.8374	Almost complete Hydroxylationconversion of proline to 4 - hydroxyproline occurs specifically at residue Pro44 of this murine protein ; the same hydroxylated residue was detected, at lower levels, in PrP ( Sc ) from the brains of scrapie - infected mice.

(308)

Cation binding and / or post - translational hydroxylation of this region of PrP may regulate its role in the physiology and pathobiology of the cell.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9961	Cation binding and / or post - translational hydroxylation of this region of ProteinPrP may regulate its role in the physiology and pathobiology of the cell.
0.9453	Cation binding and / or post - translational Hydroxylationhydroxylation of this region of PrP may regulate its role in the physiology and pathobiology of the cell.

(309)

Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B - type subunits Cdc55p and Rts1p.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B - type subunits Cdc55p and ProteinRts1p .
0.9998	Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B - type subunits ProteinCdc55p and Rts1p.

(310)

Protein phosphatase 2A ( PP2A ) is an essential eukaryotic serine / threonine phosphatase known to play important roles in cell cycle regulation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(311)

Association of different B - type targeting subunits with the heterodimeric core ( A / C ) enzyme is known to be an important mechanism of regulating PP2A activity, substrate specificity, and localization.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(312)

However, how the binding of these targeting subunits to the A / C heterodimer might be regulated is unknown.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(313)

We have used the budding yeast Saccharomyces cerevisiae as a model system to investigate the hypothesis that covalent modification of the C subunit ( Pph21p / Pph22p ) carboxyl terminus modulates PP2A complex formation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	We have used the budding yeast Saccharomyces cerevisiae as a model system to investigate the hypothesis that covalent modification of the C subunit Protein( Pph21p / Pph22p ) carboxyl terminus modulates PP2A complex formation.

(314)

Two approaches were taken.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(315)

First, S. cerevisiae cells were generated whose survival depended on the expression of different carboxyl - terminal Pph21p mutants.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	First, S. cerevisiae cells were generated whose survival depended on the expression of different carboxyl - terminal ProteinPph21p mutants.

(316)

Second, the major S. cerevisiae methyltransferase ( Ppm1p ) that catalyzes the methylation of the PP2A C subunit carboxyl - terminal leucine was identified, and cells deleted for this methyltransferase were utilized for our studies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9643	Second, the major S. cerevisiae methyltransferase Protein( Ppm1p ) that catalyzes the methylation of the PP2A C subunit carboxyl - terminal leucine was identified, and cells deleted for this methyltransferase were utilized for our studies.

(317)

Our results demonstrate that binding of the yeast B subunit, Cdc55p, to Pph21p was disrupted by either acidic substitution of potential carboxyl - terminal phosphorylation sites on Pph21p or by deletion of the gene for Ppm1p.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Our results demonstrate that binding of the yeast B subunit, Cdc55p, to Pph21p was disrupted by either acidic substitution of potential carboxyl - terminal phosphorylation sites on ProteinPph21p or by deletion of the gene for Ppm1p.
0.9996	Our results demonstrate that binding of the yeast B subunit, Cdc55p, to Pph21p was disrupted by either acidic substitution of potential carboxyl - terminal phosphorylation sites on Pph21p or by deletion of the gene for ProteinPpm1p .
0.9996	Our results demonstrate that binding of the yeast B subunit, ProteinCdc55p , to Pph21p was disrupted by either acidic substitution of potential carboxyl - terminal phosphorylation sites on Pph21p or by deletion of the gene for Ppm1p.
0.9996	Our results demonstrate that binding of the yeast B subunit, Cdc55p, to ProteinPph21p was disrupted by either acidic substitution of potential carboxyl - terminal phosphorylation sites on Pph21p or by deletion of the gene for Ppm1p.

(318)

Loss of Cdc55p association was accompanied in each case by a large reduction in binding of the yeast A subunit, Tpd3p, to Pph21p.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Loss of Cdc55p association was accompanied in each case by a large reduction in binding of the yeast A subunit, ProteinTpd3p , to Pph21p.
0.9995	Loss of ProteinCdc55p association was accompanied in each case by a large reduction in binding of the yeast A subunit, Tpd3p, to Pph21p.
0.9995	Loss of Cdc55p association was accompanied in each case by a large reduction in binding of the yeast A subunit, Tpd3p, to ProteinPph21p .

(319)

Moreover, decreased Cdc55p and Tpd3p binding invariably resulted in nocodazole sensitivity, a known phenotype of CDC55 or TPD3 deletion.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Moreover, decreased Cdc55p and Tpd3p binding invariably resulted in nocodazole sensitivity, a known phenotype of CDC55 or ProteinTPD3 deletion.
0.9998	Moreover, decreased ProteinCdc55p and Tpd3p binding invariably resulted in nocodazole sensitivity, a known phenotype of CDC55 or TPD3 deletion.
0.9997	Moreover, decreased Cdc55p and Tpd3p binding invariably resulted in nocodazole sensitivity, a known phenotype of ProteinCDC55 or TPD3 deletion.
0.9997	Moreover, decreased Cdc55p and ProteinTpd3p binding invariably resulted in nocodazole sensitivity, a known phenotype of CDC55 or TPD3 deletion.

(320)

Furthermore, loss of methylation also greatly reduced the association of another yeast B - type subunit, Rts1p.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9992	Furthermore, loss of methylation also greatly reduced the association of another yeast B - type subunit, ProteinRts1p .
0.4138	Furthermore, loss of DNA_methylationmethylation also greatly reduced the association of another yeast B - type subunit, Rts1p.

(321)

Thus, methylation of Pph21p is important for formation of PP2A trimeric and dimeric complexes, and consequently, for PP2A function.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	Thus, methylation of ProteinPph21p is important for formation of PP2A trimeric and dimeric complexes, and consequently, for PP2A function.
0.7999	Thus, Methylationmethylation of Pph21p is important for formation of PP2A trimeric and dimeric complexes, and consequently, for PP2A function.

(322)

Taken together, our results indicate that methylation and phosphorylation may be mechanisms by which the cell dynamically regulates PP2A complex formation and function.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(323)

Safety and pharmacokinetic study with escalating doses of 3 - acetyl - 7 - oxo - dehydroepiandrosterone in healthy male volunteers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(324)

OBJECTIVES : To evaluate the safety and pharmacokinetics of 3 - acetyl - 7 - oxo - DHEA ( 3beta - acetoxyandrost - 5 - ene - 7, 17 - dione ) given orally.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(325)

DESIGN : A randomized, double blind, placebo - controlled, escalating dose study.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(326)

SETTING : The Chicago Center for Clinical Research.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(327)

PARTICIPANTS : Twenty - two healthy men.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(328)

STUDY METHOD : The participants received placebo ( n = 6 ) or 3 - acetyl - 7 - oxo - DHEA ( n = 16 ) at 50 mg / d for 7 days followed by a 7 - day washout ; 100 mg / d for 7 days followed by a 7 - day washout ; and 200 mg / d for 28 days.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(329)

OUTCOME MEASURES : Safety parameters, evaluated at each dose level, included measurement of total testosterone, free testosterone, dihydrotestosterone, estradiol, cortisol, thyroxin and insulin levels.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9982	OUTCOME MEASURES : Safety parameters, evaluated at each dose level, included measurement of total testosterone, free testosterone, dihydrotestosterone, estradiol, cortisol, thyroxin and Proteininsulin levels.

(330)

Analyses for 7 - oxo - DHEA - 3beta - sulfate ( DHEA - S ), the only detectable metabolic product of the administered steroid, were conducted on plasma drawn from all subjects at 0. 25, 0. 5, 1, 2, 4, 6 and 12 hours after the final 100 mg dose of 3beta - acetyl - 7 - oxo - DHEA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(331)

RESULTS : There were no differences in the clinical laboratory values or in reported minor adverse experiences, between treatment and placebo groups.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(332)

In general, blood hormone concentrations were unaffected by the treatment with 3beta - acetyl - 7 - oxo - DHEA and remained within the normal range.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(333)

No changes in vital signs, blood chemistry or urinalysis occurred during treatment with 3beta - acetyl - 7 - oxo - DHEA compared to placebo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(334)

The administered steroid was not detected in the blood but was rapidly converted to 7 - oxo - DHEA - S, the concentrations of which were proportional to dose.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(335)

This steroid sulfate did not accumulate ; plasma concentrations 12 hours after the 3beta - acetyl - 7 - oxo - DHEA dose at 7 and 28 days on the 200 mg / d dose were 15. 8 and 16. 3 microg / L respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(336)

The mean time to peak plasma level of 7 - oxo - DHEA - S was 2. 2 hours ; the mean half life was 2. 17 hours.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(337)

The apparent clearance averaged 172 L / h, and the apparent mean volume of distribution was 540 L.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(338)

CONCLUSION : These results indicate that 3beta - acetyl - 7 - oxo - DHEA is safe and well tolerated in normal healthy men at doses up to 200 mg / d for 4 weeks.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(339)

Aureusidin synthase : a polyphenol oxidase homolog responsible for flower coloration.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9362	ProteinAureusidin synthase : a polyphenol oxidase homolog responsible for flower coloration.

(340)

Aurones are plant flavonoids that provide yellow color to the flowers of some popular ornamental plants, such as snapdragon and cosmos.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(341)

In this study, we have identified an enzyme responsible for the synthesis of aurone from chalcones in the yellow snapdragon flower.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(342)

The enzyme ( aureusidin synthase ) is a 39 - kilodalton, copper - containing glycoprotein catalyzing the hydroxylation and / or oxidative cyclization of the precursor chalcones, 2 ', 4 ', 6 ', 4 - tetrahydroxychalcone and 2 ', 4 ', 6 ', 3, 4 - pentahydroxychalcone.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9690	The enzyme Protein( aureusidin synthase ) is a 39 - kilodalton, copper - containing glycoprotein catalyzing the hydroxylation and / or oxidative cyclization of the precursor chalcones, 2 ', 4 ', 6 ', 4 - tetrahydroxychalcone and 2 ', 4 ', 6 ', 3, 4 - pentahydroxychalcone.

(343)

The complementary DNA encoding aureusidin synthase is expressed in the petals of aurone - containing varieties.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9674	The complementary DNA encoding Proteinaureusidin synthase is expressed in the petals of aurone - containing varieties.

(344)

DNA sequence analysis revealed that aureusidin synthase belongs to the plant polyphenol oxidase family, providing an unequivocal example of the function of the polyphenol oxidase homolog in plants, i. e., flower coloration.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9838	DNA sequence analysis revealed that Proteinaureusidin synthase belongs to the plant polyphenol oxidase family, providing an unequivocal example of the function of the polyphenol oxidase homolog in plants, i. e., flower coloration.

(345)

E - cadherin expression is silenced by 5'CpG island methylation in acute leukemia.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9934	ProteinE - cadherin expression is silenced by 5'CpG island methylation in acute leukemia.
0.9906	E - cadherin expression is silenced by 5'CpG island DNA_methylationmethylation in acute leukemia.
0.5636	E - cadherin expression is silenced by Entity5'CpG island methylation in acute leukemia.
0.5725	E - cadherin expression is silenced by 5 ' EntityCpG island methylation in acute leukemia.

(346)

E - Cadherin is a transmembrane glycoprotein that mediates Ca2 + - dependent intercellular adhesion in normal epithelium.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9964	ProteinE - Cadherin is a transmembrane glycoprotein that mediates Ca2 + - dependent intercellular adhesion in normal epithelium.

(347)

In tumors of epithelial origin, E - cadherin expression frequently is reduced, an event that contributes to tumor invasion and metastasis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	In tumors of epithelial origin, ProteinE - cadherin expression frequently is reduced, an event that contributes to tumor invasion and metastasis.

(348)

The role of E - cadherin in hematopoietic tissues is less clear.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	The role of ProteinE - cadherin in hematopoietic tissues is less clear.

(349)

In normal bone marrow, E - cadherin is expressed on erythroid progenitors, CD34 + stem cells, and stromal cells, where it likely contributes to intercellular interactions during hematopoiesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	In normal bone marrow, ProteinE - cadherin is expressed on erythroid progenitors, CD34 + stem cells, and stromal cells, where it likely contributes to intercellular interactions during hematopoiesis.
0.9993	In normal bone marrow, E - cadherin is expressed on erythroid progenitors, ProteinCD34 + stem cells, and stromal cells, where it likely contributes to intercellular interactions during hematopoiesis.

(350)

In this study, we used a nested - PCR approach to examine the methylation status of the E - cadherin 5'CpG island in blood and bone marrow samples from normal donors and in bone marrow from patients with acute leukemia.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9914	In this study, we used a nested - PCR approach to examine the DNA_methylationmethylation status of the E - cadherin 5'CpG island in blood and bone marrow samples from normal donors and in bone marrow from patients with acute leukemia.
0.9515	In this study, we used a nested - PCR approach to examine the methylation status of the ProteinE - cadherin 5'CpG island in blood and bone marrow samples from normal donors and in bone marrow from patients with acute leukemia.
0.7872	In this study, we used a nested - PCR approach to examine the methylation status of the E - cadherin Entity5'CpG island in blood and bone marrow samples from normal donors and in bone marrow from patients with acute leukemia.
0.8178	In this study, we used a nested - PCR approach to examine the methylation status of the E - cadherin 5 ' EntityCpG island in blood and bone marrow samples from normal donors and in bone marrow from patients with acute leukemia.
0.7121	In this study, we used a nested - PCR approach to examine the methylation status of the E - cadherin 5 ' EntityCpG island in blood and bone marrow samples from normal donors and in bone marrow from patients with acute leukemia.
0.5629	In this study, we used a nested - PCR approach to examine the methylation status of the E - cadherin Entity5'CpG island in blood and bone marrow samples from normal donors and in bone marrow from patients with acute leukemia.

(351)

In normal peripheral blood mononuclear cells and bone marrow, E - cadherin was completely unmethylated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	In normal peripheral blood mononuclear cells and bone marrow, ProteinE - cadherin was completely unmethylated.
0.9065	In normal peripheral blood mononuclear cells and bone marrow, E - cadherin was completely DNA_methylationunmethylated .

(352)

In peripheral blood mononuclear cells, expression was evident by reverse transcription - PCR.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(353)

Immunoblotting confirmed E - cadherin protein expression in two lymphoblastoid cell lines derived from normal donors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	Immunoblotting confirmed ProteinE - cadherin protein expression in two lymphoblastoid cell lines derived from normal donors.

(354)

In contrast, E - cadherin was aberrantly methylated in 4 of 4 ( 100 % ) leukemia cell lines, 14 of 44 ( 32 % ) acute myelogenous leukemias, and 18 of 33 ( 53 % ) acute lymphoblastic leukemias.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9978	In contrast, ProteinE - cadherin was aberrantly methylated in 4 of 4 ( 100 % ) leukemia cell lines, 14 of 44 ( 32 % ) acute myelogenous leukemias, and 18 of 33 ( 53 % ) acute lymphoblastic leukemias.
0.9778	In contrast, E - cadherin was aberrantly DNA_methylationmethylated in 4 of 4 ( 100 % ) leukemia cell lines, 14 of 44 ( 32 % ) acute myelogenous leukemias, and 18 of 33 ( 53 % ) acute lymphoblastic leukemias.

(355)

Genomic bisulfite sequencing of primary leukemias confirmed dense methylation across the CpG island.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9793	Genomic bisulfite sequencing of primary leukemias confirmed dense DNA_methylationmethylation across the CpG island.
0.7436	Genomic bisulfite sequencing of primary leukemias confirmed dense methylation across the EntityCpG island .

(356)

Methylation was associated with loss of E - cadherin RNA and protein in leukemia cell lines and primary leukemias.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	Methylation was associated with loss of ProteinE - cadherin RNA and protein in leukemia cell lines and primary leukemias.
0.9860	DNA_methylationMethylation was associated with loss of E - cadherin RNA and protein in leukemia cell lines and primary leukemias.

(357)

Following treatment with 5 - aza - 2'- deoxycytidine, a methylated leukemia cell line expressed both E - cadherin transcript and protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	Following treatment with 5 - aza - 2'- deoxycytidine, a methylated leukemia cell line expressed both ProteinE - cadherin transcript and protein.
0.9633	Following treatment with 5 - aza - 2'- deoxycytidine, a DNA_methylationmethylated leukemia cell line expressed both E - cadherin transcript and protein.

(358)

Our results show that methylation of E - cadherin occurs commonly in acute leukemia and suggests a hypothesis for E - cadherin down - regulation in leukemogenesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	Our results show that methylation of ProteinE - cadherin occurs commonly in acute leukemia and suggests a hypothesis for E - cadherin down - regulation in leukemogenesis.
0.9991	Our results show that methylation of E - cadherin occurs commonly in acute leukemia and suggests a hypothesis for ProteinE - cadherin down - regulation in leukemogenesis.
0.9513	Our results show that DNA_methylationmethylation of E - cadherin occurs commonly in acute leukemia and suggests a hypothesis for E - cadherin down - regulation in leukemogenesis.

(359)

Defect in N - glycosylation of proteins is tissue - dependent in congenital disorders of glycosylation Ia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(360)

The biochemical hallmark of Congenital Disorders of Glycosylation ( CDG ) including type Ia is a defective N - glycosylation of serum glycoproteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(361)

Hypoglycosylated forms of alpha1 - antitrypsin have been detected by Western blot in serum from CDG Ia patients.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9882	Hypoglycosylated forms of Proteinalpha1 - antitrypsin have been detected by Western blot in serum from CDG Ia patients.
0.7849	GlycosylationHypoglycosylated forms of alpha1 - antitrypsin have been detected by Western blot in serum from CDG Ia patients.

(362)

In contrast we were not able to detect hypoglycosylation in alpha1 - antitrypsin synthesized by fibroblasts, keratinocytes, enterocytes, and leukocytes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9891	In contrast we were not able to detect hypoglycosylation in Proteinalpha1 - antitrypsin synthesized by fibroblasts, keratinocytes, enterocytes, and leukocytes.
0.8951	In contrast we were not able to detect Glycosylationhypoglycosylation in alpha1 - antitrypsin synthesized by fibroblasts, keratinocytes, enterocytes, and leukocytes.

(363)

Similarly no hypoglycosylation was detectable in a membrane - associated N - linked glycoprotein, the facilitative glucose transporter GLUT - 1 and also in serum immunoglobulin G isolated from sera of CDG Ia patients.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9983	Similarly no hypoglycosylation was detectable in a membrane - associated N - linked glycoprotein, the facilitative glucose transporter ProteinGLUT - 1 and also in serum immunoglobulin G isolated from sera of CDG Ia patients.
0.8071	Similarly no Glycosylationhypoglycosylation was detectable in a membrane - associated N - linked glycoprotein, the facilitative glucose transporter GLUT - 1 and also in serum immunoglobulin G isolated from sera of CDG Ia patients.

(364)

We conclude that the phenotypic expression of CDG Ia is tissue - dependent.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(365)

Molecular cloning and characterization of CHM1L, a novel membrane molecule similar to chondromodulin - I.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	Molecular cloning and characterization of ProteinCHM1L , a novel membrane molecule similar to chondromodulin - I.
0.9965	Molecular cloning and characterization of CHM1L, a novel membrane molecule similar to Proteinchondromodulin - I .

(366)

Chondromodulin - I ( ChM - I ) is a cartilage - specific glycoprotein that stimulates the growth of chondrocytes and inhibits the tube formation of endothelial cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9790	Chondromodulin - I Protein( ChM - I ) is a cartilage - specific glycoprotein that stimulates the growth of chondrocytes and inhibits the tube formation of endothelial cells.
0.9666	ProteinChondromodulin - I ( ChM - I ) is a cartilage - specific glycoprotein that stimulates the growth of chondrocytes and inhibits the tube formation of endothelial cells.

(367)

In the present study, we identified a novel ChM - I like molecule, designated ChM1L.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	In the present study, we identified a novel ChM - I like molecule, designated ProteinChM1L .
0.9846	In the present study, we identified a novel ProteinChM - I like molecule, designated ChM1L.

(368)

Cloning of full length cDNAs of human, mouse, and rat ChM1L revealed that ChM1L encodes 317 amino acids novel type II transmembrane protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	Cloning of full length cDNAs of human, mouse, and rat ProteinChM1L revealed that ChM1L encodes 317 amino acids novel type II transmembrane protein.
0.9998	Cloning of full length cDNAs of human, mouse, and rat ChM1L revealed that ProteinChM1L encodes 317 amino acids novel type II transmembrane protein.

(369)

ChM1L protein was expressed on the cell surface as N - glycosylated and non - N - glycosylated protein with molecular mass of 45 and 40 kDa, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9980	ProteinChM1L protein was expressed on the cell surface as N - glycosylated and non - N - glycosylated protein with molecular mass of 45 and 40 kDa, respectively.
0.9895	ChM1L protein was expressed on the cell surface as GlycosylationN - glycosylated and non - N - glycosylated protein with molecular mass of 45 and 40 kDa, respectively.
0.9871	ChM1L protein was expressed on the cell surface as N - glycosylated and Glycosylationnon - N - glycosylated protein with molecular mass of 45 and 40 kDa, respectively.

(370)

In adult mouse tissues, ChM1L mRNA was highly expressed in eye, skeletal muscle, and whole rib.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	In adult mouse tissues, ProteinChM1L mRNA was highly expressed in eye, skeletal muscle, and whole rib.

(371)

The temporal pattern of ChM1L mRNA was examined using whole embryo at day 10 to 19 of gestation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	The temporal pattern of ProteinChM1L mRNA was examined using whole embryo at day 10 to 19 of gestation.

(372)

After day 11, ChM1L mRNA was detected and its level was progressively elevated in association with development of mouse embryo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9990	After day 11, ProteinChM1L mRNA was detected and its level was progressively elevated in association with development of mouse embryo.

(373)

These data suggest that ChM1L is a novel membrane molecule which is similar to ChM - I that plays a regulatory role in eye, skeletal muscle, and development of embryo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	These data suggest that ChM1L is a novel membrane molecule which is similar to ProteinChM - I that plays a regulatory role in eye, skeletal muscle, and development of embryo.
0.9992	These data suggest that ProteinChM1L is a novel membrane molecule which is similar to ChM - I that plays a regulatory role in eye, skeletal muscle, and development of embryo.

(374)

Targeting of calsequestrin to the sarcoplasmic reticulum of skeletal muscle upon deletion of its glycosylation site.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(375)

The glycoprotein calsequestrin ( CS ) is segregated to the junctional sarcoplasmic reticulum ( jSR ) and is responsible for intraluminal Ca ( 2 + ) binding.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(376)

A chimeric CS - hemoagglutinin 1 ( HA1 ), obtained by adding the nine amino acid viral epitope hemoagglutinin to the carboxy terminal of CS and shown to be correctly segregated to skeletal muscle jSR [ A.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9886	A chimeric CS - hemoagglutinin 1 ( HA1 ), obtained by adding the nine amino acid viral epitope Proteinhemoagglutinin to the carboxy terminal of CS and shown to be correctly segregated to skeletal muscle jSR [ A.
0.9843	A chimeric CS - hemoagglutinin 1 Protein( HA1 ) , obtained by adding the nine amino acid viral epitope hemoagglutinin to the carboxy terminal of CS and shown to be correctly segregated to skeletal muscle jSR [ A.
0.9049	A chimeric ProteinCS - hemoagglutinin 1 ( HA1 ), obtained by adding the nine amino acid viral epitope hemoagglutinin to the carboxy terminal of CS and shown to be correctly segregated to skeletal muscle jSR [ A.

(377)

Nori, K.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(378)

A.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(379)

Nadalini, A.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(380)

Martini, R.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(381)

Rizzuto, A.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(382)

Villa, and P.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(383)

Volpe ( 1997 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(384)

Chimeric calsequestrin and its targeting to the junctional sarcoplasmic reticulum of skeletal muscle.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(385)

Am.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(386)

J.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(387)

Physiol. 272, C1420 - C1428 ] lends itself as a molecular tool to investigate the targeting domains of CS.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(388)

A putative targeting mechanism of CS to jSR implies glycosylation - dependent steps in the endoplasmic reticulum ( ER ) and Golgi complex.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(389)

To test this hypothesis, CS - HA1DeltaGly, a mutant in which the unique N - glycosylation site Asn316 was changed to Ile, was engineered by site - directed mutagenesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9976	To test this hypothesis, ProteinCS - HA1DeltaGly , a mutant in which the unique N - glycosylation site Asn316 was changed to Ile, was engineered by site - directed mutagenesis.

(390)

The mutant cDNA was transiently transfected in either HeLa cells, myoblasts of rat skeletal muscle primary cultures, or regenerating soleus muscle fibers of adult rats.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(391)

The expression and intracellular localization of CS - HA1DeltaGly was studied by double - labeling epifluorescence by means of antibodies against either CS, HA1, or the ryanodine receptor calcium release channel.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	The expression and intracellular localization of ProteinCS - HA1DeltaGly was studied by double - labeling epifluorescence by means of antibodies against either CS, HA1, or the ryanodine receptor calcium release channel.
0.9979	The expression and intracellular localization of CS - HA1DeltaGly was studied by double - labeling epifluorescence by means of antibodies against either CS, ProteinHA1 , or the ryanodine receptor calcium release channel.

(392)

CS - HA1DeltaGly was expressed and retained to ER and ER / sarcoplasmic reticulum of HeLa cells and myotubes, respectively, and expressed, sorted, and correctly segregated to jSR of regenerating soleus muscle fibers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9981	ProteinCS - HA1DeltaGly was expressed and retained to ER and ER / sarcoplasmic reticulum of HeLa cells and myotubes, respectively, and expressed, sorted, and correctly segregated to jSR of regenerating soleus muscle fibers.

(393)

Thus, the targeting mechanism of CS in vivo appears not to be affected by glycosylation - that is, the sorting, docking, and segregation of CS are independent of cotranslational and posttranslational glycosylation or glycosylations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(394)

Targeting of HIF - alpha to the von Hippel - Lindau ubiquitylation complex by O2 - regulated prolyl hydroxylation.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.8673	Targeting of HIF - alpha to the Proteinvon Hippel - Lindau ubiquitylation complex by O2 - regulated prolyl hydroxylation.
0.6717	Targeting of HIF - alpha to the von ProteinHippel - Lindau ubiquitylation complex by O2 - regulated prolyl hydroxylation.

(395)

Hypoxia - inducible factor ( HIF ) is a transcriptional complex that plays a central role in the regulation of gene expression by oxygen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(396)

In oxygenated and iron replete cells, HIF - alpha subunits are rapidly destroyed by a mechanism that involves ubiquitylation by the von Hippel - Lindau tumor suppressor ( pVHL ) E3 ligase complex.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9720	In oxygenated and iron replete cells, HIF - alpha subunits are rapidly destroyed by a mechanism that involves ubiquitylation by the von Hippel - Lindau tumor suppressor Protein( pVHL ) E3 ligase complex.
0.9280	In oxygenated and iron replete cells, HIF - alpha subunits are rapidly destroyed by a mechanism that involves ubiquitylation by the Proteinvon Hippel - Lindau tumor suppressor ( pVHL ) E3 ligase complex.
0.8724	In oxygenated and iron replete cells, HIF - alpha subunits are rapidly destroyed by a mechanism that involves ubiquitylation by the von ProteinHippel - Lindau tumor suppressor ( pVHL ) E3 ligase complex.
0.8001	In oxygenated and iron replete cells, HIF - alpha subunits are rapidly destroyed by a mechanism that involves Ubiquitinationubiquitylation by the von Hippel - Lindau tumor suppressor ( pVHL ) E3 ligase complex.

(397)

This process is suppressed by hypoxia and iron chelation, allowing transcriptional activation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(398)

Here we show that the interaction between human pVHL and a specific domain of the HIF - 1alpha subunit is regulated through hydroxylation of a proline residue ( HIF - 1alpha P564 ) by an enzyme we have termed HIF - alpha prolyl - hydroxylase ( HIF - PH ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9977	Here we show that the interaction between human ProteinpVHL and a specific domain of the HIF - 1alpha subunit is regulated through hydroxylation of a proline residue ( HIF - 1alpha P564 ) by an enzyme we have termed HIF - alpha prolyl - hydroxylase ( HIF - PH ).
0.9930	Here we show that the interaction between human pVHL and a specific domain of the ProteinHIF - 1alpha subunit is regulated through hydroxylation of a proline residue ( HIF - 1alpha P564 ) by an enzyme we have termed HIF - alpha prolyl - hydroxylase ( HIF - PH ).
0.9523	Here we show that the interaction between human pVHL and a specific domain of the HIF - 1alpha subunit is regulated through hydroxylation of a proline residue Protein( HIF - 1alpha P564 ) by an enzyme we have termed HIF - alpha prolyl - hydroxylase ( HIF - PH ).
0.9316	Here we show that the interaction between human pVHL and a specific domain of the HIF - 1alpha subunit is regulated through hydroxylation of a proline residue ( HIF - 1alpha EntityP564 ) by an enzyme we have termed HIF - alpha prolyl - hydroxylase ( HIF - PH ).
0.9171	Here we show that the interaction between human pVHL and a specific domain of the HIF - 1alpha subunit is regulated through Hydroxylationhydroxylation of a proline residue ( HIF - 1alpha P564 ) by an enzyme we have termed HIF - alpha prolyl - hydroxylase ( HIF - PH ).

(399)

An absolute requirement for dioxygen as a cosubstrate and iron as cofactor suggests that HIF - PH functions directly as a cellular oxygen sensor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(400)

HIFalpha targeted for VHL - mediated destruction by proline hydroxylation : implications for O2 sensing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9980	HIFalpha targeted for ProteinVHL - mediated destruction by proline hydroxylation : implications for O2 sensing.

(401)

HIF ( hypoxia - inducible factor ) is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(402)

In the presence of oxygen, HIF is targeted for destruction by an E3 ubiquitin ligase containing the von Hippel - Lindau tumor suppressor protein ( pVHL ).

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9888	In the presence of oxygen, HIF is targeted for destruction by an E3 Proteinubiquitin ligase containing the von Hippel - Lindau tumor suppressor protein ( pVHL ).
0.9856	In the presence of oxygen, HIF is targeted for destruction by an E3 ubiquitin ligase containing the von Hippel - Lindau tumor suppressor protein Protein( pVHL ) .
0.8214	In the presence of oxygen, HIF is targeted for destruction by an E3 ubiquitin ligase containing the Proteinvon Hippel - Lindau tumor suppressor protein ( pVHL ).
0.6451	In the presence of oxygen, HIF is targeted for destruction by an E3 ubiquitin ligase containing the von ProteinHippel - Lindau tumor suppressor protein ( pVHL ).

(403)

We found that human pVHL binds to a short HIF - derived peptide when a conserved proline residue at the core of this peptide is hydroxylated.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9990	We found that human ProteinpVHL binds to a short HIF - derived peptide when a conserved proline residue at the core of this peptide is hydroxylated.
0.7480	We found that human pVHL binds to a short HIF - derived peptide when a conserved proline residue at the core of this peptide is Hydroxylationhydroxylated .

(404)

Because proline hydroxylation requires molecular oxygen and Fe ( 2 + ), this protein modification may play a key role in mammalian oxygen sensing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(405)

Genetic and clinical features of human pancreatic ductal adenocarcinomas with widespread microsatellite instability.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(406)

The incidences of microsatellite instability ( MSI ) and underlying DNA mismatch repair ( MMR ) defects in pancreatic carcinogenesis have not been well established.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(407)

We analyzed 100 sporadic and 3 hereditary pancreatic ductal adenocarcinomas for MSI, and high - frequency MSI ( MSI - H ) and low - frequency MSI ( MSI - L ) tumors were further analyzed for frameshift mutations of possible target genes and for promoter methylation and mutation of DNA MMR genes, including hMLH1, hMSH2, hMSH3, and hMSH6 genes.

Precision: 85.71

Recall: 100.00

F1: 92.31

Score	Text
0.9946	We analyzed 100 sporadic and 3 hereditary pancreatic ductal adenocarcinomas for MSI, and high - frequency MSI ( MSI - H ) and low - frequency MSI ( MSI - L ) tumors were further analyzed for frameshift mutations of possible target genes and for Entitypromoter methylation and mutation of DNA MMR genes, including hMLH1, hMSH2, hMSH3, and hMSH6 genes.
0.9939	We analyzed 100 sporadic and 3 hereditary pancreatic ductal adenocarcinomas for MSI, and high - frequency MSI ( MSI - H ) and low - frequency MSI ( MSI - L ) tumors were further analyzed for frameshift mutations of possible target genes and for promoter methylation and mutation of DNA MMR genes, including hMLH1, ProteinhMSH2 , hMSH3, and hMSH6 genes.
0.9931	We analyzed 100 sporadic and 3 hereditary pancreatic ductal adenocarcinomas for MSI, and high - frequency MSI ( MSI - H ) and low - frequency MSI ( MSI - L ) tumors were further analyzed for frameshift mutations of possible target genes and for promoter methylation and mutation of DNA MMR genes, including ProteinhMLH1 , hMSH2, hMSH3, and hMSH6 genes.
0.9926	We analyzed 100 sporadic and 3 hereditary pancreatic ductal adenocarcinomas for MSI, and high - frequency MSI ( MSI - H ) and low - frequency MSI ( MSI - L ) tumors were further analyzed for frameshift mutations of possible target genes and for promoter methylation and mutation of DNA MMR genes, including hMLH1, hMSH2, ProteinhMSH3 , and hMSH6 genes.
0.9919	We analyzed 100 sporadic and 3 hereditary pancreatic ductal adenocarcinomas for MSI, and high - frequency MSI ( MSI - H ) and low - frequency MSI ( MSI - L ) tumors were further analyzed for frameshift mutations of possible target genes and for promoter methylation and mutation of DNA MMR genes, including hMLH1, hMSH2, hMSH3, and ProteinhMSH6 genes.
0.9902	We analyzed 100 sporadic and 3 hereditary pancreatic ductal adenocarcinomas for MSI, and high - frequency MSI ( MSI - H ) and low - frequency MSI ( MSI - L ) tumors were further analyzed for frameshift mutations of possible target genes and for promoter DNA_methylationmethylation and mutation of DNA MMR genes, including hMLH1, hMSH2, hMSH3, and hMSH6 genes.
0.6006	We analyzed 100 sporadic and 3 hereditary pancreatic ductal adenocarcinomas for MSI, and high - frequency MSI ( MSI - H ) and low - frequency MSI ( MSI - L ) tumors were further analyzed for frameshift mutations of possible target genes and for DNA_methylationpromoter methylation and mutation of DNA MMR genes, including hMLH1, hMSH2, hMSH3, and hMSH6 genes.

(408)

Among the 100 sporadic tumors, 13 ( 13 % ) were MSI - H, 13 ( 13 % ) were MSI - L, and 74 ( 74 % ) were microsatellite stable ( MSS ) tumors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(409)

All of the three hereditary tumors from hereditary nonpolyposis colorectal cancer ( HNPCC ) patients were MSI - H.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(410)

MSI - H tumors were significantly associated with poor differentiation and the presence of wild - type K - RAS and p53 genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	MSI - H tumors were significantly associated with poor differentiation and the presence of wild - type K - RAS and Proteinp53 genes.
0.9989	MSI - H tumors were significantly associated with poor differentiation and the presence of wild - type ProteinK - RAS and p53 genes.

(411)

Patients with MSI - H tumors had a significantly longer overall survival time than did those with MSI - L or MSS tumors ( P = 0. 0057 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(412)

Frameshift mutations of hMSH3, hMLH3, BRCA - 2, TGF - beta type II receptor, and BAX genes were detected in MSI - H tumors.

Precision: 83.33

Recall: 100.00

F1: 90.91

Score	Text
0.9990	Frameshift mutations of hMSH3, ProteinhMLH3 , BRCA - 2, TGF - beta type II receptor, and BAX genes were detected in MSI - H tumors.
0.9989	Frameshift mutations of hMSH3, hMLH3, BRCA - 2, TGF - beta type II receptor, and ProteinBAX genes were detected in MSI - H tumors.
0.9988	Frameshift mutations of ProteinhMSH3 , hMLH3, BRCA - 2, TGF - beta type II receptor, and BAX genes were detected in MSI - H tumors.
0.9721	Frameshift mutations of hMSH3, hMLH3, ProteinBRCA - 2 , TGF - beta type II receptor, and BAX genes were detected in MSI - H tumors.
0.8722	Frameshift mutations of hMSH3, hMLH3, BRCA - 2, ProteinTGF - beta type II receptor , and BAX genes were detected in MSI - H tumors.
0.7320	Frameshift mutations of hMSH3, hMLH3, BRCA - 2, TGF - beta Proteintype II receptor , and BAX genes were detected in MSI - H tumors.

(413)

Hypermethylation of the hMLH1 promoter was observed in 6 ( 46 % ) of the 13 sporadic MSI - H tumors but not in any of the 3 hereditary MSI - H tumors or 13 MSI - L tumors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9899	DNA_methylationHypermethylation of the hMLH1 promoter was observed in 6 ( 46 % ) of the 13 sporadic MSI - H tumors but not in any of the 3 hereditary MSI - H tumors or 13 MSI - L tumors.
0.9869	Hypermethylation of the hMLH1 Entitypromoter was observed in 6 ( 46 % ) of the 13 sporadic MSI - H tumors but not in any of the 3 hereditary MSI - H tumors or 13 MSI - L tumors.
0.9686	Hypermethylation of the ProteinhMLH1 promoter was observed in 6 ( 46 % ) of the 13 sporadic MSI - H tumors but not in any of the 3 hereditary MSI - H tumors or 13 MSI - L tumors.

(414)

All of the 3 HNPCC cases had germ - line hMLH1 mutation accompanied by loss of heterogeneity or other mutation in the tumor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9980	All of the 3 HNPCC cases had germ - line ProteinhMLH1 mutation accompanied by loss of heterogeneity or other mutation in the tumor.

(415)

Our results suggest that pancreatic carcinomas with MSI - H represent a distinctive oncogenic pathway because they exhibit peculiar clinical, pathological, and molecular characteristics.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(416)

Our results also suggest the principal involvement of epigenetic or genetic inactivation of the hMLH1 gene in the pathogenesis of pancreatic carcinoma with MSI - H.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	Our results also suggest the principal involvement of epigenetic or genetic inactivation of the ProteinhMLH1 gene in the pathogenesis of pancreatic carcinoma with MSI - H.

(417)

The acetylation state of human fetal hemoglobin modulates the strength of its subunit interactions : long - range effects and implications for histone interactions in the nucleosome.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9931	The acetylation state of human fetal hemoglobin modulates the strength of its subunit interactions : long - range effects and implications for Proteinhistone interactions in the nucleosome.

(418)

The source of the 70 - fold increased tetramer strength of liganded fetal hemoglobin relative to that of adult hemoglobin between pH 6. 0 and 7. 5 reported earlier [ Dumoulin et al. ( 1997 ) J.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(419)

Biol.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(420)

Chem. 272, 31326 ] has been identified as the N - terminal Gly residue of the gamma - chain, which is replaced by Val in adult hemoglobin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(421)

This was revealed by extending the study of the pH dependence of the tetramer - dimer equilibrium of these hemoglobins into the alkaline range as far as pH 9.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(422)

From pH 7. 5 to 9. 0, the 70 - fold difference in the association equilibrium constant between hemoglobins F and A lessened progressively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(423)

This behavior was attributed to the difference in the pK ( a ) 8. 1 of Gly - 1 ( gamma ) compared to the pK ( a ) 7. 1 value of Val - 1 ( beta ) of hemoglobins F and A, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(424)

Evidence for this conclusion was obtained by demonstrating that natural hemoglobin F ( 1 ), which is specifically acetylated at Gly - 1 ( gamma ) and hence unable to be protonated, behaves like HbA and not HbF in its tetramer - dimer association properties over the pH range studied.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.9895	Evidence for this conclusion was obtained by demonstrating that natural hemoglobin F ( 1 ), which is specifically Acetylationacetylated at Gly - 1 ( gamma ) and hence unable to be protonated, behaves like HbA and not HbF in its tetramer - dimer association properties over the pH range studied.
0.5746	Evidence for this conclusion was obtained by demonstrating that natural hemoglobin F ( 1 ), which is specifically acetylated at Gly - 1 ( gamma ) and hence unable to be protonated, behaves like HbA and not ProteinHbF in its tetramer - dimer association properties over the pH range studied.

(425)

An increased degree of protonation of the gamma - chain N - terminus of hemoglobin F from pH 9. 0 to 8. 0 is therefore suggested as responsible for its increased tetramer strength representing an example of transmission of a signal from its positively charged N - terminal tail to the distant subunit allosteric interface where the equilibrium constant is measured.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.7990	An increased degree of protonation of the gamma - chain N - terminus of Proteinhemoglobin F from pH 9. 0 to 8. 0 is therefore suggested as responsible for its increased tetramer strength representing an example of transmission of a signal from its positively charged N - terminal tail to the distant subunit allosteric interface where the equilibrium constant is measured.

(426)

An analogy is made between the effects of acetylation of the fetal hemoglobin tetramer on the strength of its subunit interactions and acetylation of some internal Lys residues within the N - terminal segments of the histone octamer around which DNA is wrapped in the nucleosome.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.9642	An analogy is made between the effects of acetylation of the fetal hemoglobin tetramer on the strength of its subunit interactions and acetylation of some internal Lys residues within the N - terminal segments of the Proteinhistone octamer around which DNA is wrapped in the nucleosome.
0.8934	An analogy is made between the effects of acetylation of the fetal hemoglobin tetramer on the strength of its subunit interactions and Acetylationacetylation of some internal Lys residues within the N - terminal segments of the histone octamer around which DNA is wrapped in the nucleosome.
0.8563	An analogy is made between the effects of Acetylationacetylation of the fetal hemoglobin tetramer on the strength of its subunit interactions and acetylation of some internal Lys residues within the N - terminal segments of the histone octamer around which DNA is wrapped in the nucleosome.

(427)

Synergism of Xist RNA, DNA methylation, and histone hypoacetylation in maintaining X chromosome inactivation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9980	Synergism of ProteinXist RNA, DNA methylation, and histone hypoacetylation in maintaining X chromosome inactivation.
0.9979	Synergism of Xist RNA, DNA methylation, and Proteinhistone hypoacetylation in maintaining X chromosome inactivation.
0.9572	Synergism of Xist RNA, DNA methylation, and histone Acetylationhypoacetylation in maintaining X chromosome inactivation.

(428)

Xist RNA expression, methylation of CpG islands, and hypoacetylation of histone H4 are distinguishing features of inactive X chromatin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9952	ProteinXist RNA expression, methylation of CpG islands, and hypoacetylation of histone H4 are distinguishing features of inactive X chromatin.
0.9492	Xist RNA expression, methylation of CpG islands, and Acetylationhypoacetylation of histone H4 are distinguishing features of inactive X chromatin.
0.9371	Xist RNA expression, methylation of CpG islands, and hypoacetylation of Proteinhistone H4 are distinguishing features of inactive X chromatin.

(429)

Here, we show that these silencing mechanisms act synergistically to maintain the inactive state.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(430)

Xist RNA has been shown to be essential for initiation of X inactivation, but not required for maintenance.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	ProteinXist RNA has been shown to be essential for initiation of X inactivation, but not required for maintenance.

(431)

We have developed a system in which the reactivation frequency of individual X - linked genes can be assessed quantitatively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(432)

Using a conditional mutant Xist allele, we provide direct evidence for that loss of Xist RNA destabilizes the inactive state in somatic cells, leading to an increased reactivation frequency of an X - linked GFP transgene and of the endogenous hypoxanthine phosphoribosyl transferase ( Hprt ) gene in mouse embryonic fibroblasts.

Precision: 83.33

Recall: 100.00

F1: 90.91

Score	Text
0.9992	Using a conditional mutant ProteinXist allele, we provide direct evidence for that loss of Xist RNA destabilizes the inactive state in somatic cells, leading to an increased reactivation frequency of an X - linked GFP transgene and of the endogenous hypoxanthine phosphoribosyl transferase ( Hprt ) gene in mouse embryonic fibroblasts.
0.9990	Using a conditional mutant Xist allele, we provide direct evidence for that loss of ProteinXist RNA destabilizes the inactive state in somatic cells, leading to an increased reactivation frequency of an X - linked GFP transgene and of the endogenous hypoxanthine phosphoribosyl transferase ( Hprt ) gene in mouse embryonic fibroblasts.
0.9937	Using a conditional mutant Xist allele, we provide direct evidence for that loss of Xist RNA destabilizes the inactive state in somatic cells, leading to an increased reactivation frequency of an X - linked ProteinGFP transgene and of the endogenous hypoxanthine phosphoribosyl transferase ( Hprt ) gene in mouse embryonic fibroblasts.
0.9802	Using a conditional mutant Xist allele, we provide direct evidence for that loss of Xist RNA destabilizes the inactive state in somatic cells, leading to an increased reactivation frequency of an X - linked GFP transgene and of the endogenous hypoxanthine phosphoribosyl transferase Protein( Hprt ) gene in mouse embryonic fibroblasts.
0.7822	Using a conditional mutant Xist allele, we provide direct evidence for that loss of Xist RNA destabilizes the inactive state in somatic cells, leading to an increased reactivation frequency of an X - linked GFP transgene and of the endogenous Proteinhypoxanthine phosphoribosyl transferase ( Hprt ) gene in mouse embryonic fibroblasts.
0.6631	Using a conditional mutant Xist allele, we provide direct evidence for that loss of Xist RNA destabilizes the inactive state in somatic cells, leading to an increased reactivation frequency of an X - linked GFP transgene and of the endogenous hypoxanthine Proteinphosphoribosyl transferase ( Hprt ) gene in mouse embryonic fibroblasts.

(433)

Demethylation of DNA, using 5 - azadC or by introducing a mutation in Dnmt1, and inhibition of histone hypoacetylation using trichostatin A further increases reactivation in Xist mutant fibroblasts, indicating a synergistic interaction of X chromosome silencing mechanisms.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Demethylation of DNA, using 5 - azadC or by introducing a mutation in ProteinDnmt1 , and inhibition of histone hypoacetylation using trichostatin A further increases reactivation in Xist mutant fibroblasts, indicating a synergistic interaction of X chromosome silencing mechanisms.
0.9990	Demethylation of DNA, using 5 - azadC or by introducing a mutation in Dnmt1, and inhibition of histone hypoacetylation using trichostatin A further increases reactivation in ProteinXist mutant fibroblasts, indicating a synergistic interaction of X chromosome silencing mechanisms.
0.9982	Demethylation of DNA, using 5 - azadC or by introducing a mutation in Dnmt1, and inhibition of Proteinhistone hypoacetylation using trichostatin A further increases reactivation in Xist mutant fibroblasts, indicating a synergistic interaction of X chromosome silencing mechanisms.
0.9328	Demethylation of DNA, using 5 - azadC or by introducing a mutation in Dnmt1, and inhibition of histone Acetylationhypoacetylation using trichostatin A further increases reactivation in Xist mutant fibroblasts, indicating a synergistic interaction of X chromosome silencing mechanisms.

(434)

Expression of Pichia anomala INV1 gene in Saccharomyces cerevisiae results in two different active forms of hypoglycosylated invertase.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9876	Expression of Pichia anomala ProteinINV1 gene in Saccharomyces cerevisiae results in two different active forms of hypoglycosylated invertase.
0.9655	Expression of Pichia anomala INV1 gene in Saccharomyces cerevisiae results in two different active forms of hypoglycosylated Proteininvertase .
0.7915	Expression of Pichia anomala INV1 gene in Saccharomyces cerevisiae results in two different active forms of Glycosylationhypoglycosylated invertase.
0.3612	Expression of Pichia anomala INV1 gene in Saccharomyces cerevisiae results in two different active forms of Glycosylationhypoglycosylated invertase .

(435)

The Pichia anomala invertase gene ( INV1 ) was introduced at different copy numbers into a sucrose - nonfermenting mutant of Saccharomyces cerevisiae and expressed from its own promoter sequences.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9959	The Pichia anomala invertase gene Protein( INV1 ) was introduced at different copy numbers into a sucrose - nonfermenting mutant of Saccharomyces cerevisiae and expressed from its own promoter sequences.
0.9814	The Pichia anomala Proteininvertase gene ( INV1 ) was introduced at different copy numbers into a sucrose - nonfermenting mutant of Saccharomyces cerevisiae and expressed from its own promoter sequences.

(436)

The level reached in the production of invertase by the transformants ( up to 540 units / 10 ( 10 ) cells ) was in agreement with the INV1 gene dosage.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	The level reached in the production of invertase by the transformants ( up to 540 units / 10 ( 10 ) cells ) was in agreement with the ProteinINV1 gene dosage.
0.9996	The level reached in the production of Proteininvertase by the transformants ( up to 540 units / 10 ( 10 ) cells ) was in agreement with the INV1 gene dosage.

(437)

Two forms of multimeric active and glycosylated invertase displaying different subcellular locations and molecular masses could be detected in the transformants.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	Two forms of multimeric active and glycosylated Proteininvertase displaying different subcellular locations and molecular masses could be detected in the transformants.
0.9923	Two forms of multimeric active and Glycosylationglycosylated invertase displaying different subcellular locations and molecular masses could be detected in the transformants.

(438)

One was found to be present in the culture medium and in the periplasm, and the other could only be detected inside the cell.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(439)

Each of the two heterologous forms of invertase was shown to be an oligomer composed of identical subunits.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	Each of the two heterologous forms of Proteininvertase was shown to be an oligomer composed of identical subunits.

(440)

The difference found in the apparent molecular masses of their monomers ( 81. 5 and 78. 3 kDa, respectively ) seems to be due to the size of their N - linked oligosaccharide chains ( on average 2. 4 and 1. 9 kDa, respectively ), since the number of sugar chains ( 9 ) and the molecular mass of the protein moiety ( 60. 5 kDa ) are identical in both forms.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9700	The difference found in the apparent molecular masses of their monomers ( 81. 5 and 78. 3 kDa, respectively ) seems to be due to the size of their GlycosylationN - linked oligosaccharide chains ( on average 2. 4 and 1. 9 kDa, respectively ), since the number of sugar chains ( 9 ) and the molecular mass of the protein moiety ( 60. 5 kDa ) are identical in both forms.
0.6540	The difference found in the apparent molecular masses of their monomers ( 81. 5 and 78. 3 kDa, respectively ) seems to be due to the size of their N - linked Entityoligosaccharide chains ( on average 2. 4 and 1. 9 kDa, respectively ), since the number of sugar chains ( 9 ) and the molecular mass of the protein moiety ( 60. 5 kDa ) are identical in both forms.
0.8428	The difference found in the apparent molecular masses of their monomers ( 81. 5 and 78. 3 kDa, respectively ) seems to be due to the size of their N - linked Entityoligosaccharide chains ( on average 2. 4 and 1. 9 kDa, respectively ), since the number of sugar chains ( 9 ) and the molecular mass of the protein moiety ( 60. 5 kDa ) are identical in both forms.

(441)

The shorter size of their oligosaccharides must also be the reason for the lower apparent molecular masses of the heterologous invertases when compared with the enzyme purified from P. anomala.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(442)

The hypoglycosylated invertase accumulated within the cells of the transformants to an unusual level ( up to 130 units / 10 ( 10 ) cells ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	The hypoglycosylated Proteininvertase accumulated within the cells of the transformants to an unusual level ( up to 130 units / 10 ( 10 ) cells ).
0.9436	The Glycosylationhypoglycosylated invertase accumulated within the cells of the transformants to an unusual level ( up to 130 units / 10 ( 10 ) cells ).

(443)

Such accumulation of active enzyme inside the cells, as well as its underglycosylation, could be due to intrinsic properties of the P. anomala invertase that are determined by the particular primary structure of its protein moiety.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9873	Such accumulation of active enzyme inside the cells, as well as its underglycosylation, could be due to intrinsic properties of the P. anomala Proteininvertase that are determined by the particular primary structure of its protein moiety.
0.8928	Such accumulation of active enzyme inside the cells, as well as its Glycosylationunderglycosylation , could be due to intrinsic properties of the P. anomala invertase that are determined by the particular primary structure of its protein moiety.

(444)

The histone acetyltransferase, hGCN5, interacts with and acetylates the HIV transactivator, Tat.

Precision: 100.00

Recall: 80.00

F1: 88.89

Score	Text
0.9989	The histone acetyltransferase, ProteinhGCN5 , interacts with and acetylates the HIV transactivator, Tat.
0.9973	The histone acetyltransferase, hGCN5, interacts with and acetylates the HIV transactivator, ProteinTat .
0.9253	The Proteinhistone acetyltransferase, hGCN5, interacts with and acetylates the HIV transactivator, Tat.
0.6166	The histone acetyltransferase, hGCN5, interacts with and Acetylationacetylates the HIV transactivator, Tat.
	The histone acetyltransferase, hGCN5, interacts with and Catalysisacetylates the HIV transactivator, Tat.

(445)

Factor acetyltransferase activity associated with several histone acetyltransferases plays a key role in the control of transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9885	Factor acetyltransferase activity associated with several Proteinhistone acetyltransferases plays a key role in the control of transcription.

(446)

Here we report that hGCN5, a well known histone acetyltransferase, specifically interacts with and acetylates the human immunodeficiency virus type 1 ( HIV - 1 ) transactivator protein, Tat.

Precision: 100.00

Recall: 80.00

F1: 88.89

Score	Text
0.9991	Here we report that ProteinhGCN5 , a well known histone acetyltransferase, specifically interacts with and acetylates the human immunodeficiency virus type 1 ( HIV - 1 ) transactivator protein, Tat.
0.9980	Here we report that hGCN5, a well known histone acetyltransferase, specifically interacts with and acetylates the human immunodeficiency virus type 1 ( HIV - 1 ) transactivator protein, ProteinTat .
0.9107	Here we report that hGCN5, a well known Proteinhistone acetyltransferase, specifically interacts with and acetylates the human immunodeficiency virus type 1 ( HIV - 1 ) transactivator protein, Tat.
0.6106	Here we report that hGCN5, a well known histone acetyltransferase, specifically interacts with and Acetylationacetylates the human immunodeficiency virus type 1 ( HIV - 1 ) transactivator protein, Tat.
	Here we report that hGCN5, a well known histone acetyltransferase, specifically interacts with and Catalysisacetylates the human immunodeficiency virus type 1 ( HIV - 1 ) transactivator protein, Tat.

(447)

The interaction between Tat and hGCN5 is direct and involves the acetyltransferase and the bromodomain regions of hGCN5, as well as a limited region of Tat encompassing the cysteine - rich domain of the protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	The interaction between Tat and ProteinhGCN5 is direct and involves the acetyltransferase and the bromodomain regions of hGCN5, as well as a limited region of Tat encompassing the cysteine - rich domain of the protein.
0.9997	The interaction between Tat and hGCN5 is direct and involves the acetyltransferase and the bromodomain regions of ProteinhGCN5 , as well as a limited region of Tat encompassing the cysteine - rich domain of the protein.
0.9996	The interaction between ProteinTat and hGCN5 is direct and involves the acetyltransferase and the bromodomain regions of hGCN5, as well as a limited region of Tat encompassing the cysteine - rich domain of the protein.
0.9996	The interaction between Tat and hGCN5 is direct and involves the acetyltransferase and the bromodomain regions of hGCN5, as well as a limited region of ProteinTat encompassing the cysteine - rich domain of the protein.

(448)

Tat lysines 50 and 51, target of acetylation by p300 / CBP, were also found to be acetylated by hGCN5.

Precision: 77.78

Recall: 87.50

F1: 82.35

Score	Text
0.9948	Tat lysines 50 and 51, target of acetylation by p300 / CBP, were also found to be acetylated by ProteinhGCN5 .
0.9765	Tat lysines 50 and 51, target of acetylation by Proteinp300 / CBP , were also found to be acetylated by hGCN5.
0.9588	ProteinTat lysines 50 and 51, target of acetylation by p300 / CBP, were also found to be acetylated by hGCN5.
0.9549	Tat lysines 50 and 51, target of Acetylationacetylation by p300 / CBP, were also found to be acetylated by hGCN5.
0.9433	Tat Entitylysines 50 and 51, target of acetylation by p300 / CBP, were also found to be acetylated by hGCN5.
0.9246	Tat lysines 50 and Entity51 , target of acetylation by p300 / CBP, were also found to be acetylated by hGCN5.
0.7615	Tat lysines 50 and 51, target of acetylation by p300 / CBP, were also found to be Acetylationacetylated by hGCN5.
0.6654	Tat Entitylysines 50 and 51, target of acetylation by p300 / CBP, were also found to be acetylated by hGCN5.
0.5247	Tat lysines Entity50 and 51, target of acetylation by p300 / CBP, were also found to be acetylated by hGCN5.
	Tat lysines 50 and 51, target of acetylation by p300 / CBP, were also found to be Catalysisacetylated by hGCN5.

(449)

The acetylation of these two lysines by p300 / CBP has been recently shown to stimulate Tat transcriptional activity and accordingly, we have found that hGCN5 can considerably enhance Tat - dependent transcription of the HIV - 1 long terminal repeat.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9970	The acetylation of these two lysines by p300 / CBP has been recently shown to stimulate Tat transcriptional activity and accordingly, we have found that ProteinhGCN5 can considerably enhance Tat - dependent transcription of the HIV - 1 long terminal repeat.
0.9952	The acetylation of these two lysines by p300 / CBP has been recently shown to stimulate Tat transcriptional activity and accordingly, we have found that hGCN5 can considerably enhance ProteinTat - dependent transcription of the HIV - 1 long terminal repeat.
0.9942	The acetylation of these two lysines by Proteinp300 / CBP has been recently shown to stimulate Tat transcriptional activity and accordingly, we have found that hGCN5 can considerably enhance Tat - dependent transcription of the HIV - 1 long terminal repeat.
0.9940	The Acetylationacetylation of these two lysines by p300 / CBP has been recently shown to stimulate Tat transcriptional activity and accordingly, we have found that hGCN5 can considerably enhance Tat - dependent transcription of the HIV - 1 long terminal repeat.
0.9935	The acetylation of these two lysines by p300 / CBP has been recently shown to stimulate ProteinTat transcriptional activity and accordingly, we have found that hGCN5 can considerably enhance Tat - dependent transcription of the HIV - 1 long terminal repeat.
0.9825	The acetylation of these two Entitylysines by p300 / CBP has been recently shown to stimulate Tat transcriptional activity and accordingly, we have found that hGCN5 can considerably enhance Tat - dependent transcription of the HIV - 1 long terminal repeat.

(450)

These data highlight the importance of the acetylation of lysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to acetylate Tat on the same site.

Precision: 90.00

Recall: 90.00

F1: 90.00

Score	Text
0.9933	These data highlight the importance of the acetylation of lysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, ProteinGCN5 and p300 / CBP, converge to acetylate Tat on the same site.
0.9855	These data highlight the importance of the acetylation of lysines 50 and 51 in the function of ProteinTat , since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to acetylate Tat on the same site.
0.9854	These data highlight the importance of the Acetylationacetylation of lysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to acetylate Tat on the same site.
0.9808	These data highlight the importance of the acetylation of lysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and Proteinp300 / CBP , converge to acetylate Tat on the same site.
0.9730	These data highlight the importance of the acetylation of lysines 50 and 51 in the function of Tat, since different Proteinhistone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to acetylate Tat on the same site.
0.9638	These data highlight the importance of the acetylation of lysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to acetylate ProteinTat on the same site.
0.9603	These data highlight the importance of the acetylation of Entitylysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to acetylate Tat on the same site.
0.9145	These data highlight the importance of the acetylation of lysines 50 and Entity51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to acetylate Tat on the same site.
0.6727	These data highlight the importance of the acetylation of lysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to Acetylationacetylate Tat on the same site.
0.8143	These data highlight the importance of the acetylation of Entitylysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to acetylate Tat on the same site.
	These data highlight the importance of the acetylation of lysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to Catalysisacetylate Tat on the same site.

(451)

Hydroxylating activity of frog epidermis tyrosinase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9858	Hydroxylating activity of frog epidermis Proteintyrosinase .

(452)

Trypsin activated in a similar way both the tyrosine hydroxylase and the dopa - oxidasa activities of frog epidermis tyrosinase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9951	Trypsin activated in a similar way both the tyrosine hydroxylase and the dopa - oxidasa activities of frog epidermis Proteintyrosinase .
0.9591	Trypsin activated in a similar way both the Proteintyrosine hydroxylase and the dopa - oxidasa activities of frog epidermis tyrosinase.

(453)

Several electron donors reduced or eliminated the lag period for the hydroxylating enzyme. 4 x 10 ( - 5 ) M dopa was particularly effective, but without affecting the stationary activity after lag period.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(454)

Tyrosine hydroxylase had KM = 2. 6 X 10 ( - 3 ) M for tyrosine and 2 x 10 ( - 3 ) M dopa was a competitive inhibitor with Ki = 5 x 10 ( - 4 ) M.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9577	ProteinTyrosine hydroxylase had KM = 2. 6 X 10 ( - 3 ) M for tyrosine and 2 x 10 ( - 3 ) M dopa was a competitive inhibitor with Ki = 5 x 10 ( - 4 ) M.

(455)

The enzyme was inactivated during its actuation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(456)

Data on thermal denaturation were similar to other obtained from dopa oxidase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(457)

Our results tend to confirm our previous hypothesis that the activatory process of the enzyme is accompanied by a spatial unfolding of the enzyme molecule.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(458)

Canine COL1A2 mutation resulting in C - terminal truncation of pro - alpha2 ( I ) and severe osteogenesis imperfecta.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9957	Canine ProteinCOL1A2 mutation resulting in C - terminal truncation of pro - alpha2 ( I ) and severe osteogenesis imperfecta.

(459)

RNA and type I collagen were analyzed from cultured skin fibroblasts of a Beagle puppy with fractures consistent with type III osteogenesis imperfecta ( OI ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(460)

In a nonisotopic RNAse cleavage assay ( NIRCA ), the proband's RNA had a unique cleavage pattern in the region of COL1A2 encoding the C - propeptide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	In a nonisotopic RNAse cleavage assay ( NIRCA ), the proband's RNA had a unique cleavage pattern in the region of ProteinCOL1A2 encoding the C - propeptide.
0.9987	In a nonisotopic RNAse cleavage assay ( NIRCA ), the proband's RNA had a unique cleavage pattern in the region of COL1A2 encoding the ProteinC - propeptide .

(461)

DNA sequence analyses identified a mutation in which nucleotides 3991 - 3994 ( " CTAG " ) were replaced with " TGTCATTGG. " The first seven bases of the inserted sequence were identical to nucleotides 4002 - 4008 of the normal canine COL1A2 sequence.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9901	DNA sequence analyses identified a mutation in which nucleotides 3991 - 3994 ( " CTAG " ) were replaced with " TGTCATTGG. " The first seven bases of the inserted sequence were identical to nucleotides 4002 - 4008 of the normal canine ProteinCOL1A2 sequence.

(462)

The resulting frameshift changed 30 amino acids and introduced a premature stop codon.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(463)

Reverse - transcription polymerase chain reaction ( RT - PCR ) with primers flanking the mutation site amplified two complementary DNA ( cDNA ) fragments for the proband and a single product for the control.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(464)

Restriction enzyme digestions also were consistent with a heterozygous mutation in the proband.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(465)

Type I procollagen labeled with [ 3H ] proline was analyzed by sodium dodecyl sulfate - polyacrylamide gel electrophoresis ( SDS - PAGE ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(466)

Increased density of pC - alpha2 ( I ) suggested comigration with the similarly sized pro - alpha2 ( I ) derived from the mutant allele.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9980	Increased density of ProteinpC - alpha2 ( I ) suggested comigration with the similarly sized pro - alpha2 ( I ) derived from the mutant allele.
0.9897	Increased density of pC - alpha2 ( I ) suggested comigration with the similarly sized Proteinpro - alpha2 ( I ) derived from the mutant allele.

(467)

Furthermore, a - chains were overhydroxylated and the ratio of alpha1 ( I ) : alpha2 ( I ) was 3. 2 : 1, consistent with the presence of alpha1 ( I ) homotrimers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9948	Furthermore, a - chains were overhydroxylated and the ratio of Proteinalpha1 ( I ) : alpha2 ( I ) was 3. 2 : 1, consistent with the presence of alpha1 ( I ) homotrimers.
0.9905	Furthermore, a - chains were overhydroxylated and the ratio of alpha1 ( I ) : alpha2 ( I ) was 3. 2 : 1, consistent with the presence of Proteinalpha1 ( I ) homotrimers.
0.2319	Furthermore, a - chains were Hydroxylationoverhydroxylated and the ratio of alpha1 ( I ) : alpha2 ( I ) was 3. 2 : 1, consistent with the presence of alpha1 ( I ) homotrimers.

(468)

Analyses of COL1A2 and type I collagen were both consistent with the described heterozygous mutation affecting the pro - alpha2 ( I ) C - propeptide and confirmed a diagnosis of OI.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	Analyses of ProteinCOL1A2 and type I collagen were both consistent with the described heterozygous mutation affecting the pro - alpha2 ( I ) C - propeptide and confirmed a diagnosis of OI.
0.9913	Analyses of COL1A2 and type I collagen were both consistent with the described heterozygous mutation affecting the Proteinpro - alpha2 ( I ) C - propeptide and confirmed a diagnosis of OI.

(469)

Linking global histone acetylation to the transcription enhancement of X - chromosomal genes in Drosophila males.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9969	Linking global Proteinhistone acetylation to the transcription enhancement of X - chromosomal genes in Drosophila males.
0.9966	Linking global histone Acetylationacetylation to the transcription enhancement of X - chromosomal genes in Drosophila males.

(470)

It has become well established for several genes that targeting of histone acetylation to promoters is required for the activation of transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9966	It has become well established for several genes that targeting of Proteinhistone acetylation to promoters is required for the activation of transcription.
0.9949	It has become well established for several genes that targeting of histone Acetylationacetylation to promoters is required for the activation of transcription.

(471)

In contrast, global patterns of acetylation have not been ascribed to any particular regulatory function.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9376	In contrast, global patterns of Acetylationacetylation have not been ascribed to any particular regulatory function.

(472)

In Drosophila, a specific modification of H4, acetylation at lysine 16, is enriched at hundreds of sites on the male X chromosome due to the activity of the male - specific lethal ( MSL ) dosage compensation complex.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9982	In Drosophila, a specific modification of ProteinH4 , acetylation at lysine 16, is enriched at hundreds of sites on the male X chromosome due to the activity of the male - specific lethal ( MSL ) dosage compensation complex.
0.9936	In Drosophila, a specific modification of H4, Acetylationacetylation at lysine 16, is enriched at hundreds of sites on the male X chromosome due to the activity of the male - specific lethal ( MSL ) dosage compensation complex.
0.9415	In Drosophila, a specific modification of H4, acetylation at Entitylysine 16 , is enriched at hundreds of sites on the male X chromosome due to the activity of the male - specific lethal ( MSL ) dosage compensation complex.
0.5570	In Drosophila, a specific modification of H4, acetylation at Entitylysine 16, is enriched at hundreds of sites on the male X chromosome due to the activity of the male - specific lethal ( MSL ) dosage compensation complex.

(473)

Utilizing chromatin immunoprecipitation, we have determined that H4Ac16 is present along the entire length of X - linked genes targeted by the MSL complex with relatively modest levels of acetylation at the promoter regions and high levels in the middle and / or 3'end of the transcription units.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9858	Utilizing chromatin immunoprecipitation, we have determined that H4Ac16 is present along the entire length of X - linked genes targeted by the MSL complex with relatively modest levels of Acetylationacetylation at the promoter regions and high levels in the middle and / or 3'end of the transcription units.
0.9683	Utilizing chromatin immunoprecipitation, we have determined that ProteinH4Ac16 is present along the entire length of X - linked genes targeted by the MSL complex with relatively modest levels of acetylation at the promoter regions and high levels in the middle and / or 3'end of the transcription units.
	Utilizing chromatin immunoprecipitation, we have determined that EntityH4Ac16 is present along the entire length of X - linked genes targeted by the MSL complex with relatively modest levels of acetylation at the promoter regions and high levels in the middle and / or 3'end of the transcription units.

(474)

We propose that global acetylation by the MSL complex increases the expression of X - linked genes by facilitating transcription elongation rather than by enhancing promoter accessibility.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9508	We propose that global Acetylationacetylation by the MSL complex increases the expression of X - linked genes by facilitating transcription elongation rather than by enhancing promoter accessibility.

(475)

We have also determined that H4Ac16 is absent from a region of the X chromosome that includes a gene known to be dosage - compensated by a MSL - independent mechanism.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9967	We have also determined that ProteinH4Ac16 is absent from a region of the X chromosome that includes a gene known to be dosage - compensated by a MSL - independent mechanism.

(476)

This study represents the first biochemical interpretation of the very large body of cytological observations on the chromosomal distribution of the MSL complex.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(477)

Correlation between histone lysine methylation and developmental changes at the chicken beta - globin locus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9937	Correlation between histone lysine methylation and developmental changes at the chicken Proteinbeta - globin locus.
0.9655	Correlation between Proteinhistone lysine methylation and developmental changes at the chicken beta - globin locus.
0.9525	Correlation between histone lysine Methylationmethylation and developmental changes at the chicken beta - globin locus.
0.9095	Correlation between histone Entitylysine methylation and developmental changes at the chicken beta - globin locus.

(478)

Methylation of histones at specific residues plays an important role in transcriptional regulation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9852	Methylation of Proteinhistones at specific residues plays an important role in transcriptional regulation.
0.9722	MethylationMethylation of histones at specific residues plays an important role in transcriptional regulation.

(479)

Chromatin immunoprecipitation of dimethylated lysine 9 on histone H3 across 53 kilobases of the chicken beta - globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 methylation and acetylation.

Precision: 87.50

Recall: 100.00

F1: 93.33

Score	Text
0.9962	Chromatin immunoprecipitation of dimethylated lysine 9 on histone H3 across 53 kilobases of the chicken Proteinbeta - globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 methylation and acetylation.
0.9866	Chromatin immunoprecipitation of dimethylated lysine 9 on histone H3 across 53 kilobases of the chicken beta - globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 Methylationmethylation and acetylation.
0.9472	Chromatin immunoprecipitation of dimethylated lysine 9 on histone H3 across 53 kilobases of the chicken beta - globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated Entitylysine 9 methylation and acetylation.
0.8892	Chromatin immunoprecipitation of dimethylated lysine 9 on histone H3 across 53 kilobases of the chicken beta - globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 methylation and Acetylationacetylation .
0.8396	Chromatin immunoprecipitation of dimethylated lysine 9 on Proteinhistone H3 across 53 kilobases of the chicken beta - globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 methylation and acetylation.
0.7354	Chromatin immunoprecipitation of Methylationdimethylated lysine 9 on histone H3 across 53 kilobases of the chicken beta - globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 methylation and acetylation.
0.7054	Chromatin immunoprecipitation of dimethylated Entitylysine 9 on histone H3 across 53 kilobases of the chicken beta - globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 methylation and acetylation.
0.7563	Chromatin immunoprecipitation of dimethylated lysine 9 on histone H3 across 53 kilobases of the chicken beta - globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated Entitylysine 9 methylation and acetylation.

(480)

Lysine 9 is methylated most over constitutive condensed chromatin and developmentally inactive globin genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9681	Lysine 9 is Methylationmethylated most over constitutive condensed chromatin and developmentally inactive globin genes.
0.8260	EntityLysine 9 is methylated most over constitutive condensed chromatin and developmentally inactive globin genes.

(481)

In contrast, lysine 4 methylation of histone H3 correlates with H3 acetylation.

Precision: 100.00

Recall: 80.00

F1: 88.89

Score	Text
0.9724	In contrast, lysine 4 methylation of histone H3 correlates with H3 Acetylationacetylation .
0.9719	In contrast, lysine 4 methylation of histone H3 correlates with ProteinH3 acetylation.
0.9194	In contrast, lysine 4 methylation of Proteinhistone H3 correlates with H3 acetylation.
0.8455	In contrast, lysine 4 Methylationmethylation of histone H3 correlates with H3 acetylation.
	In contrast, Entitylysine 4 methylation of histone H3 correlates with H3 acetylation.

(482)

These results lead us to propose a mechanism by which the insulator in the beta - globin locus can protect the globin genes from being silenced by adjacent condensed chromatin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9923	These results lead us to propose a mechanism by which the insulator in the Proteinbeta - globin locus can protect the globin genes from being silenced by adjacent condensed chromatin.

(483)

Purification and characterization of a soluble bioactive amino - terminal extracellular domain of the human thyrotropin receptor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9753	Purification and characterization of a soluble bioactive amino - terminal extracellular domain of the human Proteinthyrotropin receptor .

(484)

The amino - terminal ectodomain of the human TSH receptor has been expressed at the surface of CHO cells as a glycosylphosphatidylinositol - anchored molecule containing a 10 - residue histidine tag close to its C terminus.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9469	The amino - terminal ectodomain of the human ProteinTSH receptor has been expressed at the surface of CHO cells as a glycosylphosphatidylinositol - anchored molecule containing a 10 - residue histidine tag close to its C terminus.
0.7491	The amino - terminal ectodomain of the human TSH receptor has been expressed at the surface of CHO cells as a Glycosylationglycosylphosphatidylinositol - anchored molecule containing a 10 - residue histidine tag close to its C terminus.
	The amino - terminal ectodomain of the human TSH receptor has been expressed at the surface of CHO cells as a Entityglycosylphosphatidylinositol - anchored molecule containing a 10 - residue histidine tag close to its C terminus.

(485)

The soluble ectodomain could be released from the cells by treatment with a glycosylphosphatidylinositol - phospholipase C and purified to apparent homogeneity by cobalt - Sepharose chromatography.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.8493	The soluble ectodomain could be released from the cells by treatment with a Proteinglycosylphosphatidylinositol - phospholipase C and purified to apparent homogeneity by cobalt - Sepharose chromatography.

(486)

Two nanomoles of material was obtained, which was suitable for analysis by mass spectrometry.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(487)

This allowed the identification of four out of the six potential N - glycosylation sites as being effectively glycosylated.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9916	This allowed the identification of four out of the six potential N - glycosylation sites as being effectively Glycosylationglycosylated .
0.7807	This allowed the identification of four out of the six potential EntityN - glycosylation sites as being effectively glycosylated.
0.6101	This allowed the identification of four out of the six potential EntityN - glycosylation sites as being effectively glycosylated.

(488)

A proportion of the purified soluble ectodomain displayed specific binding of ( 125 ) I - labeled TSH, allowing for the first time performance of classical saturation binding experiments.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9913	A proportion of the purified soluble ectodomain displayed specific binding of ( 125 ) I - labeled ProteinTSH , allowing for the first time performance of classical saturation binding experiments.

(489)

Two classes of high - affinity binding sites were identified : site A, K ( d ) 0. 014 nM ; site B, K ( d ) 0. 83 nM.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(490)

The significance of site A, whose affinity is much higher than for the holoreceptor at the surface of intact cells, remains to be clarified.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(491)

The purified ectodomain was capable of inhibiting efficiently the thyroid stimulating activity of immunoglobulins from patients with Graves'disease.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(492)

It allowed computation of the amounts of these immunoglobulins in patient's serum, giving values up to 10 microg / mL.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(493)

Contrary to all currently available assays, the soluble ectodomain of the TSH receptor purified in a functionally competent conformation allows direct studies of its interactions with TSH and autoantibodies and opens the way to structural studies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9976	Contrary to all currently available assays, the soluble ectodomain of the TSH receptor purified in a functionally competent conformation allows direct studies of its interactions with ProteinTSH and autoantibodies and opens the way to structural studies.
0.9790	Contrary to all currently available assays, the soluble ectodomain of the ProteinTSH receptor purified in a functionally competent conformation allows direct studies of its interactions with TSH and autoantibodies and opens the way to structural studies.

(494)

HIF - 1alpha binding to VHL is regulated by stimulus - sensitive proline hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	ProteinHIF - 1alpha binding to VHL is regulated by stimulus - sensitive proline hydroxylation.
0.9982	HIF - 1alpha binding to ProteinVHL is regulated by stimulus - sensitive proline hydroxylation.
0.9823	HIF - 1alpha binding to VHL is regulated by stimulus - sensitive Entityproline hydroxylation.
0.9502	HIF - 1alpha binding to VHL is regulated by stimulus - sensitive proline Hydroxylationhydroxylation .

(495)

Hypoxia - inducible factor - 1alpha ( HIF - 1alpha ) is a global transcriptional regulator of the hypoxic response.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9507	Hypoxia - inducible factor - 1alpha Protein( HIF - 1alpha ) is a global transcriptional regulator of the hypoxic response.
0.7768	ProteinHypoxia - inducible factor - 1alpha ( HIF - 1alpha ) is a global transcriptional regulator of the hypoxic response.

(496)

Under normoxic conditions, HIF - 1alpha is recognized by the von Hippel - Lindau tumor - suppressor protein ( VHL ), a component of an E3 ubiquitin ligase complex.

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9971	Under normoxic conditions, ProteinHIF - 1alpha is recognized by the von Hippel - Lindau tumor - suppressor protein ( VHL ), a component of an E3 ubiquitin ligase complex.
0.9899	Under normoxic conditions, HIF - 1alpha is recognized by the von Hippel - Lindau tumor - suppressor protein ( VHL ), a component of an E3 Proteinubiquitin ligase complex.
0.9859	Under normoxic conditions, HIF - 1alpha is recognized by the von Hippel - Lindau tumor - suppressor protein Protein( VHL ) , a component of an E3 ubiquitin ligase complex.
0.7529	Under normoxic conditions, HIF - 1alpha is recognized by the Proteinvon Hippel - Lindau tumor - suppressor protein ( VHL ), a component of an E3 ubiquitin ligase complex.
0.5662	Under normoxic conditions, HIF - 1alpha is recognized by the von ProteinHippel - Lindau tumor - suppressor protein ( VHL ), a component of an E3 ubiquitin ligase complex.

(497)

This interaction thereby promotes the rapid degradation of HIF - 1alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9990	This interaction thereby promotes the rapid degradation of ProteinHIF - 1alpha .

(498)

Under hypoxic conditions, HIF - 1alpha is stabilized.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9985	Under hypoxic conditions, ProteinHIF - 1alpha is stabilized.

(499)

We have previously shown that VHL binds in a hypoxia - sensitive manner to a 27 - aa segment of HIF - 1alpha, and that this regulation depends on a posttranslational modification of HIF - 1alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	We have previously shown that ProteinVHL binds in a hypoxia - sensitive manner to a 27 - aa segment of HIF - 1alpha, and that this regulation depends on a posttranslational modification of HIF - 1alpha.
0.9988	We have previously shown that VHL binds in a hypoxia - sensitive manner to a 27 - aa segment of HIF - 1alpha, and that this regulation depends on a posttranslational modification of ProteinHIF - 1alpha .
0.9987	We have previously shown that VHL binds in a hypoxia - sensitive manner to a 27 - aa segment of ProteinHIF - 1alpha , and that this regulation depends on a posttranslational modification of HIF - 1alpha.

(500)

Through a combination of in vivo coimmunoprecipitation assays using VHL and a panel of point mutants of HIF - 1alpha in this region, as well as MS and in vitro binding assays, we now provide evidence that this modification, which occurs under normoxic conditions, is hydroxylation of Pro - 564 of HIF - 1alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9974	Through a combination of in vivo coimmunoprecipitation assays using ProteinVHL and a panel of point mutants of HIF - 1alpha in this region, as well as MS and in vitro binding assays, we now provide evidence that this modification, which occurs under normoxic conditions, is hydroxylation of Pro - 564 of HIF - 1alpha.
0.9960	Through a combination of in vivo coimmunoprecipitation assays using VHL and a panel of point mutants of ProteinHIF - 1alpha in this region, as well as MS and in vitro binding assays, we now provide evidence that this modification, which occurs under normoxic conditions, is hydroxylation of Pro - 564 of HIF - 1alpha.
0.9951	Through a combination of in vivo coimmunoprecipitation assays using VHL and a panel of point mutants of HIF - 1alpha in this region, as well as MS and in vitro binding assays, we now provide evidence that this modification, which occurs under normoxic conditions, is hydroxylation of Pro - 564 of ProteinHIF - 1alpha .
0.9949	Through a combination of in vivo coimmunoprecipitation assays using VHL and a panel of point mutants of HIF - 1alpha in this region, as well as MS and in vitro binding assays, we now provide evidence that this modification, which occurs under normoxic conditions, is hydroxylation of EntityPro - 564 of HIF - 1alpha.
0.9673	Through a combination of in vivo coimmunoprecipitation assays using VHL and a panel of point mutants of HIF - 1alpha in this region, as well as MS and in vitro binding assays, we now provide evidence that this modification, which occurs under normoxic conditions, is Hydroxylationhydroxylation of Pro - 564 of HIF - 1alpha.

(501)

The data furthermore show that this proline hydroxylation is the primary regulator of VHL binding.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9959	The data furthermore show that this proline hydroxylation is the primary regulator of ProteinVHL binding.

(502)

2B4 ( CD244 ) and CS1 : novel members of the CD2 subset of the immunoglobulin superfamily molecules expressed on natural killer cells and other leukocytes.

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9992	2B4 ( CD244 ) and CS1 : novel members of the ProteinCD2 subset of the immunoglobulin superfamily molecules expressed on natural killer cells and other leukocytes.
0.9981	2B4 ( CD244 ) and ProteinCS1 : novel members of the CD2 subset of the immunoglobulin superfamily molecules expressed on natural killer cells and other leukocytes.
0.9837	Protein2B4 ( CD244 ) and CS1 : novel members of the CD2 subset of the immunoglobulin superfamily molecules expressed on natural killer cells and other leukocytes.
0.8892	2B4 Protein( CD244 ) and CS1 : novel members of the CD2 subset of the immunoglobulin superfamily molecules expressed on natural killer cells and other leukocytes.
0.6130	Protein2B4 ( CD244 ) and CS1 : novel members of the CD2 subset of the immunoglobulin superfamily molecules expressed on natural killer cells and other leukocytes.

(503)

2B4 is a member of the CD2 subset of the immunoglobulin superfamily molecules expressed on natural killer ( NK ) cells and other leukocytes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	Protein2B4 is a member of the CD2 subset of the immunoglobulin superfamily molecules expressed on natural killer ( NK ) cells and other leukocytes.
0.9990	2B4 is a member of the ProteinCD2 subset of the immunoglobulin superfamily molecules expressed on natural killer ( NK ) cells and other leukocytes.

(504)

It is the high affinity ligand for CD48.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	It is the high affinity ligand for ProteinCD48 .

(505)

Engagement of 2B4 on NK - cell surfaces with specific antibodies or CD48 can trigger cell - mediated cytotoxicity, interferon - gamma secretion, phosphoinositol turnover and NK - cell invasiveness.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	Engagement of 2B4 on NK - cell surfaces with specific antibodies or ProteinCD48 can trigger cell - mediated cytotoxicity, interferon - gamma secretion, phosphoinositol turnover and NK - cell invasiveness.
0.9989	Engagement of Protein2B4 on NK - cell surfaces with specific antibodies or CD48 can trigger cell - mediated cytotoxicity, interferon - gamma secretion, phosphoinositol turnover and NK - cell invasiveness.
0.9979	Engagement of 2B4 on NK - cell surfaces with specific antibodies or CD48 can trigger cell - mediated cytotoxicity, Proteininterferon - gamma secretion, phosphoinositol turnover and NK - cell invasiveness.

(506)

The function of 2B4 in CD8 + T cells and myeloid cells remains unknown.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	The function of 2B4 in ProteinCD8 + T cells and myeloid cells remains unknown.
0.9988	The function of Protein2B4 in CD8 + T cells and myeloid cells remains unknown.

(507)

The cytoplasmic domain of 2B4 contains unique tyrosine motifs ( TxYxxV / I ) that associate with src homology 2 domain - containing protein or signaling lymphocyte activation molecule ( SLAM ) - associated protein, whose mutation is the underlying genetic defect in the X - linked lymphoproliferative disease ( XLPD ).

Precision: 50.00

Recall: 50.00

F1: 50.00

Score	Text
0.9992	The cytoplasmic domain of Protein2B4 contains unique tyrosine motifs ( TxYxxV / I ) that associate with src homology 2 domain - containing protein or signaling lymphocyte activation molecule ( SLAM ) - associated protein, whose mutation is the underlying genetic defect in the X - linked lymphoproliferative disease ( XLPD ).
0.6419	The cytoplasmic domain of 2B4 contains unique tyrosine motifs ( TxYxxV / I ) that associate with Proteinsrc homology 2 domain - containing protein or signaling lymphocyte activation molecule ( SLAM ) - associated protein, whose mutation is the underlying genetic defect in the X - linked lymphoproliferative disease ( XLPD ).
	The cytoplasmic domain of 2B4 contains unique tyrosine motifs ( TxYxxV / I ) that associate with src homology 2 domain - containing protein or Proteinsignaling lymphocyte activation molecule ( SLAM ) - associated protein , whose mutation is the underlying genetic defect in the X - linked lymphoproliferative disease ( XLPD ).

(508)

Impaired signaling via 2B4 and SLAM is implicated in the immunopathogenesis of XLPD.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9798	Impaired signaling via Protein2B4 and SLAM is implicated in the immunopathogenesis of XLPD.

(509)

CS1 is a novel member of the CD2 subset that contains two of the unique tyrosine motifs present in 2B4 and SLAM.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	CS1 is a novel member of the ProteinCD2 subset that contains two of the unique tyrosine motifs present in 2B4 and SLAM.
0.9994	ProteinCS1 is a novel member of the CD2 subset that contains two of the unique tyrosine motifs present in 2B4 and SLAM.
0.9895	CS1 is a novel member of the CD2 subset that contains two of the unique tyrosine motifs present in Protein2B4 and SLAM.

(510)

Signaling through 2B4, CS1 and other members of the CD2 subset may play a major role in the regulation of NK cells and other leukocyte functions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	Signaling through 2B4, CS1 and other members of the ProteinCD2 subset may play a major role in the regulation of NK cells and other leukocyte functions.
0.9994	Signaling through 2B4, ProteinCS1 and other members of the CD2 subset may play a major role in the regulation of NK cells and other leukocyte functions.
0.9982	Signaling through Protein2B4 , CS1 and other members of the CD2 subset may play a major role in the regulation of NK cells and other leukocyte functions.

(511)

N - glycosylation of CRF receptor type 1 is important for its ligand - specific interaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9840	GlycosylationN - glycosylation of CRF receptor type 1 is important for its ligand - specific interaction.
0.8376	N - glycosylation of ProteinCRF receptor type 1 is important for its ligand - specific interaction.

(512)

The corticotropin - releasing factor ( CRF ) receptor type 1 ( CRFR1 ) contains five potential N - glycosylation sites : N38, N45, N78, N90, and N98.

Precision: 100.00

Recall: 50.00

F1: 66.67

Score	Text
0.9446	The corticotropin - releasing factor ( CRF ) receptor type 1 Protein( CRFR1 ) contains five potential N - glycosylation sites : N38, N45, N78, N90, and N98.
	The Proteincorticotropin - releasing factor ( CRF ) receptor type 1 ( CRFR1 ) contains five potential N - glycosylation sites : N38, N45, N78, N90, and N98.

(513)

Cells expressing CRFR1 were treated with tunicamycin to block receptor glycosylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	Cells expressing ProteinCRFR1 were treated with tunicamycin to block receptor glycosylation.

(514)

The nonglycosylated receptor did not bind the radioligand and had a decreased cAMP stimulation potency in response to CRF.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	The nonglycosylated receptor did not bind the radioligand and had a decreased cAMP stimulation potency in response to ProteinCRF .

(515)

To determine which of the polysaccharide chain ( s ) is / are involved in ligand interaction, the polysaccharide chains were deleted using site - directed mutagenesis of the glycosylation consensus, N - X - S / T.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(516)

Two sets of mutations were performed for each glycosylation site : N to Q and S / T to A, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(517)

The single mutants Q38, Q45, Q78, Q90, Q98, A40, A47, A80, A92, and A100 and the double mutants A40 / A47 and A80 / A100 were well expressed, bound CRF, sauvagine ( SVG ), and urotensin - I ( UTS - I ) with a normal affinity, and increased cAMP accumulation with a high efficiency.

Precision: 71.43

Recall: 100.00

F1: 83.33

Score	Text
0.9935	The single mutants Q38, Q45, Q78, Q90, Q98, A40, A47, A80, A92, and A100 and the double mutants A40 / A47 and A80 / A100 were well expressed, bound ProteinCRF , sauvagine ( SVG ), and urotensin - I ( UTS - I ) with a normal affinity, and increased cAMP accumulation with a high efficiency.
0.9903	The single mutants Q38, Q45, Q78, Q90, Q98, A40, A47, A80, A92, and A100 and the double mutants A40 / A47 and A80 / A100 were well expressed, bound CRF, sauvagine ( SVG ), and Proteinurotensin - I ( UTS - I ) with a normal affinity, and increased cAMP accumulation with a high efficiency.
0.9861	The single mutants Q38, Q45, Q78, Q90, Q98, A40, A47, A80, A92, and A100 and the double mutants A40 / A47 and A80 / A100 were well expressed, bound CRF, sauvagine ( SVG ), and urotensin - I Protein( UTS - I ) with a normal affinity, and increased cAMP accumulation with a high efficiency.
0.9698	The single mutants Q38, Q45, Q78, Q90, Q98, A40, A47, A80, A92, and A100 and the double mutants A40 / A47 and A80 / A100 were well expressed, bound CRF, sauvagine Protein( SVG ) , and urotensin - I ( UTS - I ) with a normal affinity, and increased cAMP accumulation with a high efficiency.
0.9151	The single mutants Q38, Q45, Q78, Q90, Q98, A40, A47, A80, A92, and A100 and the double mutants A40 / A47 and A80 / A100 were well expressed, bound CRF, Proteinsauvagine ( SVG ), and urotensin - I ( UTS - I ) with a normal affinity, and increased cAMP accumulation with a high efficiency.
0.6232	The single mutants Q38, Q45, Q78, Q90, Q98, A40, A47, A80, A92, and A100 and the double mutants A40 / A47 and A80 / A100 were well expressed, bound CRF, sauvagine ( SVG ), and Proteinurotensin - I ( UTS - I ) with a normal affinity, and increased cAMP accumulation with a high efficiency.
0.6147	The single mutants Q38, Q45, Q78, Q90, Q98, A40, A47, A80, A92, and A100 and the double mutants A40 / A47 and A80 / A100 were well expressed, bound CRF, Proteinsauvagine ( SVG ) , and urotensin - I ( UTS - I ) with a normal affinity, and increased cAMP accumulation with a high efficiency.

(518)

In contrast, the combined mutations A80 / A92 / A100, A40 / A80 / A92 / A100, and A40 / A47 / A80 / A92 / A100 had low levels of expression, did not bind the radioligand, and had a decreased cAMP stimulation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(519)

These data indicate the requirement for three or more polysaccharide chains for normal CRFR1 function.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	These data indicate the requirement for three or more polysaccharide chains for normal ProteinCRFR1 function.

(520)

Reduced rates of gene loss, gene silencing, and gene mutation in Dnmt1 - deficient embryonic stem cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Reduced rates of gene loss, gene silencing, and gene mutation in ProteinDnmt1 - deficient embryonic stem cells.

(521)

Tumor suppressor gene inactivation is a crucial event in oncogenesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(522)

Gene inactivation mechanisms include events resulting in loss of heterozygosity ( LOH ), gene mutation, and transcriptional silencing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(523)

The contribution of each of these different pathways varies among tumor suppressor genes and by cancer type.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(524)

The factors that influence the relative utilization of gene inactivation pathways are poorly understood.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(525)

In this study, we describe a detailed quantitative analysis of the three major gene inactivation mechanisms for a model gene at two different genomic integration sites in mouse embryonic stem ( ES ) cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(526)

In addition, we targeted the major DNA methyltransferase gene, Dnmt1, to investigate the relative contribution of DNA methylation to these various competing gene inactivation pathways.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	In addition, we targeted the major DNA methyltransferase gene, ProteinDnmt1 , to investigate the relative contribution of DNA methylation to these various competing gene inactivation pathways.

(527)

Our data show that gene loss is the predominant mode of inactivation of a herpes simplex virus thymidine kinase neomycin phosphotransferase reporter gene ( HSV - TKNeo ) at the two integration sites tested and that this event is significantly reduced in Dnmt1 - deficient cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	Our data show that gene loss is the predominant mode of inactivation of a herpes simplex virus thymidine kinase neomycin phosphotransferase reporter gene ( HSV - TKNeo ) at the two integration sites tested and that this event is significantly reduced in ProteinDnmt1 - deficient cells.
0.5653	Our data show that gene loss is the predominant mode of inactivation of a herpes simplex virus Proteinthymidine kinase neomycin phosphotransferase reporter gene ( HSV - TKNeo ) at the two integration sites tested and that this event is significantly reduced in Dnmt1 - deficient cells.

(528)

Gene silencing by promoter methylation requires Dnmt1, suggesting that the expression of Dnmt3a and Dnmt3b alone in ES cells is insufficient to achieve effective gene silencing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Gene silencing by promoter methylation requires ProteinDnmt1 , suggesting that the expression of Dnmt3a and Dnmt3b alone in ES cells is insufficient to achieve effective gene silencing.
0.9997	Gene silencing by promoter methylation requires Dnmt1, suggesting that the expression of ProteinDnmt3a and Dnmt3b alone in ES cells is insufficient to achieve effective gene silencing.
0.9997	Gene silencing by promoter methylation requires Dnmt1, suggesting that the expression of Dnmt3a and ProteinDnmt3b alone in ES cells is insufficient to achieve effective gene silencing.

(529)

We used a novel assay to show that missense mutation rates are also substantially reduced in Dnmt1 - deficient cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	We used a novel assay to show that missense mutation rates are also substantially reduced in ProteinDnmt1 - deficient cells.

(530)

This is the first direct demonstration that DNA methylation affects point mutation rates in mammalian cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(531)

Surprisingly, the fraction of CpG transition mutations was not reduced in Dnmt1 - deficient cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Surprisingly, the fraction of CpG transition mutations was not reduced in ProteinDnmt1 - deficient cells.

(532)

Finally, we show that methyl group - deficient growth conditions do not cause an increase in missense mutation rates in Dnmt1 - proficient cells, as predicted by methyltransferase - mediated mutagenesis models.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Finally, we show that methyl group - deficient growth conditions do not cause an increase in missense mutation rates in ProteinDnmt1 - proficient cells, as predicted by methyltransferase - mediated mutagenesis models.

(533)

We conclude that Dnmt1 deficiency and the accompanying genomic DNA hypomethylation result in a reduction of three major pathways of gene inactivation in our model system.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	We conclude that ProteinDnmt1 deficiency and the accompanying genomic DNA hypomethylation result in a reduction of three major pathways of gene inactivation in our model system.

(534)

Induction of hepatitis C virus E1 envelope protein - specific immune response can be enhanced by mutation of N - glycosylation sites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	Induction of hepatitis C virus ProteinE1 envelope protein - specific immune response can be enhanced by mutation of N - glycosylation sites.

(535)

Deglycosylation of viral glycoproteins has been shown to influence the number of available epitopes and to modulate immune recognition of antigens.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(536)

We investigated the role played by N - glycans in the immunogenicity of hepatitis C virus ( HCV ) E1 envelope glycoprotein, a naturally poor immunogen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	We investigated the role played by N - glycans in the immunogenicity of hepatitis C virus ( HCV ) ProteinE1 envelope glycoprotein, a naturally poor immunogen.

(537)

Eight plasmids were engineered, encoding E1 protein mutants in which the four N - linked glycosylation sites of the protein were mutated separately or in combination.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Eight plasmids were engineered, encoding ProteinE1 protein mutants in which the four N - linked glycosylation sites of the protein were mutated separately or in combination.

(538)

In vitro expression studies showed an influence of N - linked glycosylation on expression efficiency, instability, and / or secretion of the mutated proteins.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.6787	In vitro expression studies showed an influence of GlycosylationN - linked glycosylation on expression efficiency, instability, and / or secretion of the mutated proteins.
0.7581	In vitro expression studies showed an influence of N - linked Glycosylationglycosylation on expression efficiency, instability, and / or secretion of the mutated proteins.

(539)

Immunogenicity of the E1 mutants was studied in BALB / c mice following intramuscular and intraepidermal injection of the plasmids.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	Immunogenicity of the ProteinE1 mutants was studied in BALB / c mice following intramuscular and intraepidermal injection of the plasmids.

(540)

Whereas some mutations had no or only minor effects on the antibody titers induced, mutation of the fourth glycosylation site ( N4 ) significantly enhanced the anti - E1 humoral response in terms of both seroconversion rates and antibody titers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	Whereas some mutations had no or only minor effects on the antibody titers induced, mutation of the fourth glycosylation site ( N4 ) significantly enhanced the Proteinanti - E1 humoral response in terms of both seroconversion rates and antibody titers.

(541)

Moreover, antibody induced by the N4 mutant was able to recognize HCV - like particles with higher titers than those induced by the wild - type construct.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(542)

Epitope mapping indicated that the E1 mutant antigens induced antibody directed at two major domains : one, located at amino acids ( aa ) 313 to 332, which is known to be reactive with sera from HCV patients, and a second one, located in the N - terminal domain of E1 ( aa 192 to 226 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Epitope mapping indicated that the ProteinE1 mutant antigens induced antibody directed at two major domains : one, located at amino acids ( aa ) 313 to 332, which is known to be reactive with sera from HCV patients, and a second one, located in the N - terminal domain of E1 ( aa 192 to 226 ).
0.9969	Epitope mapping indicated that the E1 mutant antigens induced antibody directed at two major domains : one, located at amino acids ( aa ) 313 to 332, which is known to be reactive with sera from HCV patients, and a second one, located in the N - terminal domain of ProteinE1 ( aa 192 to 226 ).

(543)

Analysis of the induced immune cellular response confirmed the induction of gamma interferon - producing cells by all mutants, albeit to different levels.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9356	Analysis of the induced immune cellular response confirmed the induction of Proteingamma interferon - producing cells by all mutants, albeit to different levels.
0.7378	Analysis of the induced immune cellular response confirmed the induction of gamma Proteininterferon - producing cells by all mutants, albeit to different levels.

(544)

These results show that N - linked glycosylation can limit the antibody response to the HCV E1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9993	These results show that N - linked glycosylation can limit the antibody response to the HCV ProteinE1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.
0.9191	These results show that GlycosylationN - linked glycosylation can limit the antibody response to the HCV E1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.
0.9275	These results show that N - linked Glycosylationglycosylation can limit the antibody response to the HCV E1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.
0.7148	These results show that GlycosylationN - linked glycosylation can limit the antibody response to the HCV E1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.

(545)

Absence of post - translational aspartyl beta - hydroxylation of epidermal growth factor domains in mice leads to developmental defects and an increased incidence of intestinal neoplasia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(546)

The BAH genomic locus encodes three distinct proteins : junctin, humbug, and BAH.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	The BAH genomic locus encodes three distinct proteins : Proteinjunctin , humbug, and BAH.
0.9993	The BAH genomic locus encodes three distinct proteins : junctin, humbug, and ProteinBAH .
0.9988	The ProteinBAH genomic locus encodes three distinct proteins : junctin, humbug, and BAH.
0.9987	The BAH genomic locus encodes three distinct proteins : junctin, Proteinhumbug , and BAH.

(547)

All three proteins share common exons, but differ significantly based upon the use of alternative terminal exons.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(548)

The biological roles of BAH and humbug and their functional relationship to junctin remain unclear.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	The biological roles of ProteinBAH and humbug and their functional relationship to junctin remain unclear.
0.9990	The biological roles of BAH and humbug and their functional relationship to Proteinjunctin remain unclear.
0.9990	The biological roles of BAH and Proteinhumbug and their functional relationship to junctin remain unclear.

(549)

To evaluate the role of BAH in vivo, the catalytic domain of BAH was specifically targeted such that the coding regions of junctin and humbug remained undisturbed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	To evaluate the role of ProteinBAH in vivo, the catalytic domain of BAH was specifically targeted such that the coding regions of junctin and humbug remained undisturbed.
0.9995	To evaluate the role of BAH in vivo, the catalytic domain of ProteinBAH was specifically targeted such that the coding regions of junctin and humbug remained undisturbed.
0.9993	To evaluate the role of BAH in vivo, the catalytic domain of BAH was specifically targeted such that the coding regions of junctin and Proteinhumbug remained undisturbed.
0.9990	To evaluate the role of BAH in vivo, the catalytic domain of BAH was specifically targeted such that the coding regions of Proteinjunctin and humbug remained undisturbed.

(550)

BAH null mice lack measurable BAH protein in several tissues, lack aspartyl beta - hydroxylase activity in liver preparations, and exhibit no hydroxylation of the epidermal growth factor ( EGF ) domain of clotting Factor X.

Precision: 80.00

Recall: 80.00

F1: 80.00

Score	Text
0.9816	BAH null mice lack measurable ProteinBAH protein in several tissues, lack aspartyl beta - hydroxylase activity in liver preparations, and exhibit no hydroxylation of the epidermal growth factor ( EGF ) domain of clotting Factor X.
0.9787	ProteinBAH null mice lack measurable BAH protein in several tissues, lack aspartyl beta - hydroxylase activity in liver preparations, and exhibit no hydroxylation of the epidermal growth factor ( EGF ) domain of clotting Factor X.
0.9504	BAH null mice lack measurable BAH protein in several tissues, lack aspartyl beta - hydroxylase activity in liver preparations, and exhibit no Hydroxylationhydroxylation of the epidermal growth factor ( EGF ) domain of clotting Factor X.
0.9359	BAH null mice lack measurable BAH protein in several tissues, lack aspartyl beta - hydroxylase activity in liver preparations, and exhibit no hydroxylation of the epidermal growth factor ( EGF ) domain of clotting ProteinFactor X .
0.6169	BAH null mice lack measurable BAH protein in several tissues, lack Proteinaspartyl beta - hydroxylase activity in liver preparations, and exhibit no hydroxylation of the epidermal growth factor ( EGF ) domain of clotting Factor X.
	BAH null mice lack measurable BAH protein in several tissues, lack aspartyl beta - hydroxylase activity in liver preparations, and exhibit no hydroxylation of the Entityepidermal growth factor ( EGF ) domain of clotting Factor X.

(551)

In addition to reduced fertility in females, BAH null mice display several developmental defects including syndactyly, facial dysmorphology, and a mild defect in hard palate formation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	In addition to reduced fertility in females, ProteinBAH null mice display several developmental defects including syndactyly, facial dysmorphology, and a mild defect in hard palate formation.

(552)

The developmental defects present in BAH null mice are similar to defects observed in knock - outs and hypomorphs of the Notch ligand Serrate - 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9982	The developmental defects present in ProteinBAH null mice are similar to defects observed in knock - outs and hypomorphs of the Notch ligand Serrate - 2.
0.9975	The developmental defects present in BAH null mice are similar to defects observed in knock - outs and hypomorphs of the Notch ligand ProteinSerrate - 2 .

(553)

In this work, beta - hydroxylation of Asp residues in EGF domains is demonstrated for a soluble form of a Notch ligand, human Jagged - 1.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9909	In this work, beta - hydroxylation of Asp residues in EGF domains is demonstrated for a soluble form of a Notch ligand, human ProteinJagged - 1 .
0.9170	In this work, Hydroxylationbeta - hydroxylation of Asp residues in EGF domains is demonstrated for a soluble form of a Notch ligand, human Jagged - 1.
0.8369	In this work, beta - hydroxylation of EntityAsp residues in EGF domains is demonstrated for a soluble form of a Notch ligand, human Jagged - 1.
0.5882	In this work, beta - hydroxylation of EntityAsp residues in EGF domains is demonstrated for a soluble form of a Notch ligand, human Jagged - 1.

(554)

These results along with recent reports that another post - translational modification of EGF domains in Notch gene family members ( glycosylation by Fringe ) alters Notch pathway signaling, lends credence to the suggestion that aspartyl beta - hydroxylation may represent another post - translational modification of EGF domains that can modulate Notch pathway signaling.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(555)

Previous work has demonstrated increased levels of BAH in certain tumor tissues and a role for BAH in tumorigenesis has been proposed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9990	Previous work has demonstrated increased levels of ProteinBAH in certain tumor tissues and a role for BAH in tumorigenesis has been proposed.
0.9985	Previous work has demonstrated increased levels of BAH in certain tumor tissues and a role for ProteinBAH in tumorigenesis has been proposed.

(556)

The role of hydroxylase in tumor formation was tested directly by crossing BAH KO mice with an intestinal tumor model, APCmin mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9690	The role of hydroxylase in tumor formation was tested directly by crossing ProteinBAH KO mice with an intestinal tumor model, APCmin mice.

(557)

Surprisingly, BAH null / APCmin mice show a statistically significant increase in both intestinal polyp size and number when compared with BAH wild - type / APCmin controls.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Surprisingly, ProteinBAH null / APCmin mice show a statistically significant increase in both intestinal polyp size and number when compared with BAH wild - type / APCmin controls.
0.9988	Surprisingly, BAH null / APCmin mice show a statistically significant increase in both intestinal polyp size and number when compared with ProteinBAH wild - type / APCmin controls.

(558)

These results suggest that, in contrast to expectations, loss of BAH catalytic activity may promote tumor formation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	These results suggest that, in contrast to expectations, loss of ProteinBAH catalytic activity may promote tumor formation.

(559)

Molecular cloning of ESET, a novel histone H3 - specific methyltransferase that interacts with ERG transcription factor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	Molecular cloning of ProteinESET , a novel histone H3 - specific methyltransferase that interacts with ERG transcription factor.
0.9966	Molecular cloning of ESET, a novel histone H3 - specific methyltransferase that interacts with ProteinERG transcription factor.
0.9323	Molecular cloning of ESET, a novel Proteinhistone H3 - specific methyltransferase that interacts with ERG transcription factor.

(560)

The ets - related gene erg encodes a transcription factor that is implicated in the control of cell growth and differentiation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	The ets - related gene Proteinerg encodes a transcription factor that is implicated in the control of cell growth and differentiation.

(561)

To identify interacting partners of ERG, we screened a yeast two - hybrid cDNA library constructed from mouse hematopoietic cells using the N - terminal region of ERG as a bait.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	To identify interacting partners of ERG, we screened a yeast two - hybrid cDNA library constructed from mouse hematopoietic cells using the N - terminal region of ProteinERG as a bait.
0.9998	To identify interacting partners of ProteinERG , we screened a yeast two - hybrid cDNA library constructed from mouse hematopoietic cells using the N - terminal region of ERG as a bait.

(562)

We isolated a 4. 6 kb full - length mouse cDNA encoding a 1307 - amino acid protein migrating as a 180 kD band, which was termed ESET ( ERG - associated protein with SET domain ).

Precision: 100.00

Recall: 50.00

F1: 66.67

Score	Text
0.9931	We isolated a 4. 6 kb full - length mouse cDNA encoding a 1307 - amino acid protein migrating as a 180 kD band, which was termed ProteinESET ( ERG - associated protein with SET domain ).
	We isolated a 4. 6 kb full - length mouse cDNA encoding a 1307 - amino acid protein migrating as a 180 kD band, which was termed ESET Protein( ERG - associated protein with SET domain ) .

(563)

ESET is 92 % identical to the human protein SETDB1 ( SET domain, bifurcated 1 ).

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9991	ProteinESET is 92 % identical to the human protein SETDB1 ( SET domain, bifurcated 1 ).
0.9771	ESET is 92 % identical to the human protein ProteinSETDB1 ( SET domain, bifurcated 1 ).
	ESET is 92 % identical to the human protein SETDB1 Protein( SET domain, bifurcated 1 ) .

(564)

The interaction between ESET and ERG was supported by in vitro pull - down using glutathione S - transferase ( GST ) fusion protein, by transfection and co - immunoprecipitation experiments, and by association of endogenous SETDB1 with ERG.

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9998	The interaction between ESET and ERG was supported by in vitro pull - down using glutathione S - transferase ( GST ) fusion protein, by transfection and co - immunoprecipitation experiments, and by association of endogenous ProteinSETDB1 with ERG.
0.9998	The interaction between ESET and ProteinERG was supported by in vitro pull - down using glutathione S - transferase ( GST ) fusion protein, by transfection and co - immunoprecipitation experiments, and by association of endogenous SETDB1 with ERG.
0.9998	The interaction between ESET and ERG was supported by in vitro pull - down using glutathione S - transferase ( GST ) fusion protein, by transfection and co - immunoprecipitation experiments, and by association of endogenous SETDB1 with ProteinERG .
0.9997	The interaction between ProteinESET and ERG was supported by in vitro pull - down using glutathione S - transferase ( GST ) fusion protein, by transfection and co - immunoprecipitation experiments, and by association of endogenous SETDB1 with ERG.
0.6251	The interaction between ESET and ERG was supported by in vitro pull - down using Proteinglutathione S - transferase ( GST ) fusion protein, by transfection and co - immunoprecipitation experiments, and by association of endogenous SETDB1 with ERG.

(565)

Since ESET possesses evolutionarily conserved SET, preSET, and postSET domains implicated in histone methylation, we tested the ability of ESET to methylate core histones.

Precision: 83.33

Recall: 100.00

F1: 90.91

Score	Text
0.9988	Since ProteinESET possesses evolutionarily conserved SET, preSET, and postSET domains implicated in histone methylation, we tested the ability of ESET to methylate core histones.
0.9986	Since ESET possesses evolutionarily conserved SET, preSET, and postSET domains implicated in histone methylation, we tested the ability of ProteinESET to methylate core histones.
0.9815	Since ESET possesses evolutionarily conserved SET, preSET, and postSET domains implicated in histone methylation, we tested the ability of ESET to methylate core Proteinhistones .
0.9770	Since ESET possesses evolutionarily conserved SET, preSET, and postSET domains implicated in Proteinhistone methylation, we tested the ability of ESET to methylate core histones.
0.9692	Since ESET possesses evolutionarily conserved SET, preSET, and postSET domains implicated in histone Methylationmethylation , we tested the ability of ESET to methylate core histones.
0.8421	Since ESET possesses evolutionarily conserved SET, preSET, and postSET domains implicated in histone methylation, we tested the ability of ESET to Methylationmethylate core histones.

(566)

The results of these studies demonstrated that ESET is a histone H3 - specific methyltransferase, and that mutations within ESET abolished its methyltransferase activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	The results of these studies demonstrated that ProteinESET is a histone H3 - specific methyltransferase, and that mutations within ESET abolished its methyltransferase activity.
0.9993	The results of these studies demonstrated that ESET is a histone H3 - specific methyltransferase, and that mutations within ProteinESET abolished its methyltransferase activity.
0.9535	The results of these studies demonstrated that ESET is a Proteinhistone H3 - specific methyltransferase, and that mutations within ESET abolished its methyltransferase activity.

(567)

Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ESET - mediated histone methylation.

Precision: 75.00

Recall: 60.00

F1: 66.67

Score	Text
0.9992	Together, these findings raise the possibility that transcription factor ProteinERG may participate in transcriptional regulation through ESET - mediated histone methylation.
0.9505	Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ESET - mediated histone Methylationmethylation .
0.6013	Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ProteinESET - mediated histone methylation.
0.4680	Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ProteinESET - mediated histone methylation.
	Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ESET - mediated Proteinhistone methylation.
	Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through CatalysisESET - mediated histone methylation.

(568)

Regulation of HIF by the von Hippel - Lindau tumour suppressor : implications for cellular oxygen sensing.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.8033	Regulation of HIF by the Proteinvon Hippel - Lindau tumour suppressor : implications for cellular oxygen sensing.
0.5814	Regulation of HIF by the von ProteinHippel - Lindau tumour suppressor : implications for cellular oxygen sensing.

(569)

Hypoxia - inducible factor ( HIF ) is central in coordinating many of the transcriptional adaptations to hypoxia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(570)

Composed of a heterodimer of alpha and beta subunits, the alpha subunit is rapidly degraded in normoxia, leading to inactivation of the hypoxic response.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(571)

Many models for a molecular oxygen sensor regulating this system have been proposed, but an important finding has been the ability to mimic hypoxia by chelation or substitution of iron.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(572)

A key insight has been the recognition that HIF - alpha is targeted for degradation by the ubiquitin - proteasome pathway through binding to the von Hippel - Lindau tumour suppressor protein ( pVHL ), which forms the recognition component of an E3 ubiquitin ligase complex leading to ubiquitylation of HIF - alpha.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9990	A key insight has been the recognition that HIF - alpha is targeted for degradation by the Proteinubiquitin - proteasome pathway through binding to the von Hippel - Lindau tumour suppressor protein ( pVHL ), which forms the recognition component of an E3 ubiquitin ligase complex leading to ubiquitylation of HIF - alpha.
0.9953	A key insight has been the recognition that HIF - alpha is targeted for degradation by the ubiquitin - proteasome pathway through binding to the von Hippel - Lindau tumour suppressor protein ( pVHL ), which forms the recognition component of an E3 Proteinubiquitin ligase complex leading to ubiquitylation of HIF - alpha.
0.9911	A key insight has been the recognition that HIF - alpha is targeted for degradation by the ubiquitin - proteasome pathway through binding to the von Hippel - Lindau tumour suppressor protein Protein( pVHL ) , which forms the recognition component of an E3 ubiquitin ligase complex leading to ubiquitylation of HIF - alpha.
0.8337	A key insight has been the recognition that HIF - alpha is targeted for degradation by the ubiquitin - proteasome pathway through binding to the Proteinvon Hippel - Lindau tumour suppressor protein ( pVHL ), which forms the recognition component of an E3 ubiquitin ligase complex leading to ubiquitylation of HIF - alpha.
0.9391	A key insight has been the recognition that HIF - alpha is targeted for degradation by the ubiquitin - proteasome pathway through binding to the von Hippel - Lindau tumour suppressor protein ( pVHL ), which forms the recognition component of an E3 ubiquitin ligase complex leading to Ubiquitinationubiquitylation of HIF - alpha.
0.6917	A key insight has been the recognition that HIF - alpha is targeted for degradation by the ubiquitin - proteasome pathway through binding to the von ProteinHippel - Lindau tumour suppressor protein ( pVHL ), which forms the recognition component of an E3 ubiquitin ligase complex leading to ubiquitylation of HIF - alpha.

(573)

Importantly, the classical features of regulation by iron and oxygen availability are reflected in regulation of the HIF - alpha / pVHL interaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9892	Importantly, the classical features of regulation by iron and oxygen availability are reflected in regulation of the ProteinHIF - alpha / pVHL interaction.

(574)

It has recently been shown that HIF - alpha undergoes an iron - and oxygen - dependent modification before it can interact with pVHL, and that this results in hydroxylation of at least one prolyl residue ( HIF - 1alpha, Pro 564 ).

Precision: 71.43

Recall: 100.00

F1: 83.33

Score	Text
0.9975	It has recently been shown that HIF - alpha undergoes an iron - and oxygen - dependent modification before it can interact with ProteinpVHL , and that this results in hydroxylation of at least one prolyl residue ( HIF - 1alpha, Pro 564 ).
0.9639	It has recently been shown that HIF - alpha undergoes an iron - and oxygen - dependent modification before it can interact with pVHL, and that this results in Hydroxylationhydroxylation of at least one prolyl residue ( HIF - 1alpha, Pro 564 ).
0.8824	It has recently been shown that HIF - alpha undergoes an iron - and oxygen - dependent modification before it can interact with pVHL, and that this results in hydroxylation of at least one Entityprolyl residue ( HIF - 1alpha, Pro 564 ).
0.8342	It has recently been shown that HIF - alpha undergoes an iron - and oxygen - dependent modification before it can interact with pVHL, and that this results in hydroxylation of at least one prolyl residue Protein( HIF - 1alpha , Pro 564 ).
0.5357	It has recently been shown that HIF - alpha undergoes an iron - and oxygen - dependent modification before it can interact with pVHL, and that this results in hydroxylation of at least one prolyl residue ( HIF - 1alpha, EntityPro 564 ) .
0.6449	It has recently been shown that HIF - alpha undergoes an iron - and oxygen - dependent modification before it can interact with pVHL, and that this results in hydroxylation of at least one Entityprolyl residue ( HIF - 1alpha, Pro 564 ).
0.5868	It has recently been shown that HIF - alpha undergoes an iron - and oxygen - dependent modification before it can interact with pVHL, and that this results in hydroxylation of at least one prolyl residue ( HIF - 1alpha, Pro Entity564 ) .

(575)

This modification is catalysed by an enzyme termed HIF - prolyl hydroxylase ( HIF - PH ), and compatible with all previously described prolyl - 4 - hydroxylases HIF - PH also requires 2 - oxoglutarate as a cosubstrate.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.5810	This modification is catalysed by an enzyme termed ProteinHIF - prolyl hydroxylase ( HIF - PH ), and compatible with all previously described prolyl - 4 - hydroxylases HIF - PH also requires 2 - oxoglutarate as a cosubstrate.

(576)

The key position of this hydroxylation in the degradation pathway of HIF - alpha, together with its requirement for molecular dioxygen as a co - substrate, provides the potential for HIF - PH to function directly as a cellular oxygen sensor.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.6904	The key position of this Hydroxylationhydroxylation in the degradation pathway of HIF - alpha, together with its requirement for molecular dioxygen as a co - substrate, provides the potential for HIF - PH to function directly as a cellular oxygen sensor.

(577)

However, the ability of these enzyme ( s ) to account for the full range of physiological regulation displayed by the HIF system remains to be defined.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(578)

Promoter methylation status of the DNA repair genes hMLH1 and MGMT in gastric carcinoma and metaplastic mucosa.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9876	Promoter DNA_methylationmethylation status of the DNA repair genes hMLH1 and MGMT in gastric carcinoma and metaplastic mucosa.
0.9793	Promoter methylation status of the DNA repair genes ProteinhMLH1 and MGMT in gastric carcinoma and metaplastic mucosa.
0.9762	EntityPromoter methylation status of the DNA repair genes hMLH1 and MGMT in gastric carcinoma and metaplastic mucosa.
0.9760	Promoter methylation status of the DNA repair genes hMLH1 and ProteinMGMT in gastric carcinoma and metaplastic mucosa.

(579)

Hypermethylation of CpG islands in the promoter region is associated with the silencing of a variety of tumor suppressor genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(580)

DNA repair genes human Mut L homologue 1 ( hMLH1 ) and O ( 6 ) - methylguanine - DNA methyltransferase ( MGMT ) have been shown to be hypermethylated in certain carcinomas.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9822	DNA repair genes human Mut L homologue 1 ( hMLH1 ) and O ( 6 ) - methylguanine - DNA methyltransferase ( MGMT ) have been shown to be DNA_methylationhypermethylated in certain carcinomas.
0.9377	DNA repair genes human Mut L homologue 1 Protein( hMLH1 ) and O ( 6 ) - methylguanine - DNA methyltransferase ( MGMT ) have been shown to be hypermethylated in certain carcinomas.
0.9351	DNA repair genes human Mut L homologue 1 ( hMLH1 ) and O ( 6 ) - methylguanine - DNA methyltransferase Protein( MGMT ) have been shown to be hypermethylated in certain carcinomas.
0.9225	DNA repair genes human Mut L homologue 1 ( hMLH1 ) and ProteinO ( 6 ) - methylguanine - DNA methyltransferase ( MGMT ) have been shown to be hypermethylated in certain carcinomas.
0.8682	DNA repair genes human ProteinMut L homologue 1 ( hMLH1 ) and O ( 6 ) - methylguanine - DNA methyltransferase ( MGMT ) have been shown to be hypermethylated in certain carcinomas.

(581)

We studied DNA methylation of CpG islands in hMLH1 and MGMT in 50 gastric carcinomas and 10 intestinal metaplastic mucosa samples.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9966	We studied DNA methylation of CpG islands in hMLH1 and ProteinMGMT in 50 gastric carcinomas and 10 intestinal metaplastic mucosa samples.
0.9955	We studied DNA methylation of CpG islands in ProteinhMLH1 and MGMT in 50 gastric carcinomas and 10 intestinal metaplastic mucosa samples.
0.8821	We studied DNA_methylationDNA methylation of CpG islands in hMLH1 and MGMT in 50 gastric carcinomas and 10 intestinal metaplastic mucosa samples.
0.6843	We studied DNA methylation of EntityCpG islands in hMLH1 and MGMT in 50 gastric carcinomas and 10 intestinal metaplastic mucosa samples.
0.9370	We studied DNA DNA_methylationmethylation of CpG islands in hMLH1 and MGMT in 50 gastric carcinomas and 10 intestinal metaplastic mucosa samples.
0.6879	We studied DNA_methylationDNA methylation of CpG islands in hMLH1 and MGMT in 50 gastric carcinomas and 10 intestinal metaplastic mucosa samples.

(582)

We analyzed the methylation status of hMLH1 and MGMT using methylation - specific polymerase chain reaction and DNA sequencing analysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	We analyzed the methylation status of hMLH1 and ProteinMGMT using methylation - specific polymerase chain reaction and DNA sequencing analysis.
0.9984	We analyzed the methylation status of ProteinhMLH1 and MGMT using methylation - specific polymerase chain reaction and DNA sequencing analysis.
0.7930	We analyzed the DNA_methylationmethylation status of hMLH1 and MGMT using methylation - specific polymerase chain reaction and DNA sequencing analysis.

(583)

We measured protein levels of hMLH1 using Western blot and immunohistochemical analysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	We measured protein levels of ProteinhMLH1 using Western blot and immunohistochemical analysis.

(584)

CpG island hypermethylation of hMLH1 and MGMT was detected in 11 ( 22 % ) and 8 ( 16 % ) of the 50 gastric tumors, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9871	CpG island hypermethylation of hMLH1 and ProteinMGMT was detected in 11 ( 22 % ) and 8 ( 16 % ) of the 50 gastric tumors, respectively.
0.9736	CpG island hypermethylation of ProteinhMLH1 and MGMT was detected in 11 ( 22 % ) and 8 ( 16 % ) of the 50 gastric tumors, respectively.
0.9541	CpG island DNA_methylationhypermethylation of hMLH1 and MGMT was detected in 11 ( 22 % ) and 8 ( 16 % ) of the 50 gastric tumors, respectively.
0.8572	EntityCpG island hypermethylation of hMLH1 and MGMT was detected in 11 ( 22 % ) and 8 ( 16 % ) of the 50 gastric tumors, respectively.

(585)

Hypermethylation of the promoter was more common in intestinal - type gastric carcinomas than in poorly diffuse - type gastric carcinomas ( p = 0. 016 and 0. 021, respectively ; Fisher's exact test ).

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.9800	DNA_methylationHypermethylation of the promoter was more common in intestinal - type gastric carcinomas than in poorly diffuse - type gastric carcinomas ( p = 0. 016 and 0. 021, respectively ; Fisher's exact test ).
0.9627	Hypermethylation of the Entitypromoter was more common in intestinal - type gastric carcinomas than in poorly diffuse - type gastric carcinomas ( p = 0. 016 and 0. 021, respectively ; Fisher's exact test ).

(586)

However, hMLH1 promoter hypermethylation did not coincide with MGMT promoter hypermethylation except in 1 patient.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9840	However, hMLH1 promoter DNA_methylationhypermethylation did not coincide with MGMT promoter hypermethylation except in 1 patient.
0.9765	However, hMLH1 promoter hypermethylation did not coincide with ProteinMGMT promoter hypermethylation except in 1 patient.
0.9746	However, hMLH1 promoter hypermethylation did not coincide with MGMT promoter DNA_methylationhypermethylation except in 1 patient.
0.9677	However, ProteinhMLH1 promoter hypermethylation did not coincide with MGMT promoter hypermethylation except in 1 patient.
0.9603	However, hMLH1 Entitypromoter hypermethylation did not coincide with MGMT promoter hypermethylation except in 1 patient.
0.9508	However, hMLH1 promoter hypermethylation did not coincide with MGMT Entitypromoter hypermethylation except in 1 patient.

(587)

Hypermethylation of the hMLH1 promoter but not the MGMT promoter occurred in intestinal metaplastic mucosae.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9816	DNA_methylationHypermethylation of the hMLH1 promoter but not the MGMT promoter occurred in intestinal metaplastic mucosae.
0.9754	Hypermethylation of the hMLH1 promoter but not the MGMT Entitypromoter occurred in intestinal metaplastic mucosae.
0.9646	Hypermethylation of the hMLH1 Entitypromoter but not the MGMT promoter occurred in intestinal metaplastic mucosae.
0.9570	Hypermethylation of the ProteinhMLH1 promoter but not the MGMT promoter occurred in intestinal metaplastic mucosae.
0.9513	Hypermethylation of the hMLH1 promoter but not the ProteinMGMT promoter occurred in intestinal metaplastic mucosae.

(588)

Immunohistochemical analysis revealed a corresponding reduction in hMLH1 protein expression in some of the intestinal metaplastic mucosae.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9961	Immunohistochemical analysis revealed a corresponding reduction in ProteinhMLH1 protein expression in some of the intestinal metaplastic mucosae.

(589)

Our results suggest that at least two types of promoter methylation participate in the development of gastric carcinoma.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.5066	Our results suggest that at least two types of promoter DNA_methylationmethylation participate in the development of gastric carcinoma.

(590)

Tumor - specific promoter hypermethylation of hMLH1 may be an early event in carcinogenesis in the stomach.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9868	Tumor - specific promoter DNA_methylationhypermethylation of hMLH1 may be an early event in carcinogenesis in the stomach.
0.9857	Tumor - specific promoter hypermethylation of ProteinhMLH1 may be an early event in carcinogenesis in the stomach.
0.9539	Tumor - specific Entitypromoter hypermethylation of hMLH1 may be an early event in carcinogenesis in the stomach.

(591)

Histone deacetylases and cancer : causes and therapies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	ProteinHistone deacetylases and cancer : causes and therapies.

(592)

Together, histone acetyltransferases and histone deacetylases ( HDACs ) determine the acetylation status of histones.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	Together, Proteinhistone acetyltransferases and histone deacetylases ( HDACs ) determine the acetylation status of histones.
0.9993	Together, histone acetyltransferases and Proteinhistone deacetylases ( HDACs ) determine the acetylation status of histones.
0.9944	Together, histone acetyltransferases and histone deacetylases ( HDACs ) determine the acetylation status of Proteinhistones .
0.9809	Together, histone acetyltransferases and histone deacetylases ( HDACs ) determine the Acetylationacetylation status of histones.

(593)

This acetylation affects the regulation of gene expression, and inhibitors of HDACs have been found to cause growth arrest, differentiation and / or apoptosis of many tumours cells by altering the transcription of a small number of genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9544	This Acetylationacetylation affects the regulation of gene expression, and inhibitors of HDACs have been found to cause growth arrest, differentiation and / or apoptosis of many tumours cells by altering the transcription of a small number of genes.

(594)

HDAC inhibitors are proving to be an exciting therapeutic approach to cancer, but how do they exert this effect?

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(595)

Co - and posttranslational modification of the alpha ( 1B ) - adrenergic receptor : effects on receptor expression and function.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9634	Co - and posttranslational modification of the Proteinalpha ( 1B ) - adrenergic receptor : effects on receptor expression and function.

(596)

We have characterized the maturation, co - and posttranslational modifications, and functional properties of the alpha ( 1B ) - adrenergic receptor ( AR ) expressed in different mammalian cells transfected using conventional approaches or the Semliki Forest virus system.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9103	We have characterized the maturation, co - and posttranslational modifications, and functional properties of the Proteinalpha ( 1B ) - adrenergic receptor ( AR ) expressed in different mammalian cells transfected using conventional approaches or the Semliki Forest virus system.

(597)

We found that the alpha ( 1B ) - AR undergoes N - linked glycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9976	We found that the Proteinalpha ( 1B ) - AR undergoes N - linked glycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.
0.9517	We found that the alpha ( 1B ) - AR undergoes GlycosylationN - linked glycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.
0.9726	We found that the alpha ( 1B ) - AR undergoes N - linked Glycosylationglycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.
0.7185	We found that the alpha ( 1B ) - AR undergoes GlycosylationN - linked glycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.

(598)

Pulse - chase labeling experiments in BHK - 21 cells demonstrate that the alpha ( 1B ) - AR is synthesized as a 70 kDa core glycosylated precursor that is converted to the 90 kDa mature form of the receptor with a half - time of approximately 2 h.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9949	Pulse - chase labeling experiments in BHK - 21 cells demonstrate that the alpha ( 1B ) - AR is synthesized as a 70 kDa core Glycosylationglycosylated precursor that is converted to the 90 kDa mature form of the receptor with a half - time of approximately 2 h.
0.9939	Pulse - chase labeling experiments in BHK - 21 cells demonstrate that the Proteinalpha ( 1B ) - AR is synthesized as a 70 kDa core glycosylated precursor that is converted to the 90 kDa mature form of the receptor with a half - time of approximately 2 h.

(599)

N - Linked glycosylation of the alpha ( 1B ) - AR occurs at four asparagines on the N - terminus of the receptor.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9754	N - Linked Glycosylationglycosylation of the alpha ( 1B ) - AR occurs at four asparagines on the N - terminus of the receptor.
0.9583	N - Linked glycosylation of the Proteinalpha ( 1B ) - AR occurs at four asparagines on the N - terminus of the receptor.
0.6148	N - Linked glycosylation of the alpha ( 1B ) - AR occurs at Entityfour asparagines on the N - terminus of the receptor.
0.8108	N - Linked glycosylation of the alpha ( 1B ) - AR occurs at four Entityasparagines on the N - terminus of the receptor.

(600)

Mutations of the N - linked glycosylation sites did not have a significant effect on receptor function or expression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(601)

Surprisingly, receptor mutants lacking N - linked glycosylation migrated as heterogeneous bands in SDS - PAGE.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(602)

Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9890	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the Proteinalpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.9365	Our findings demonstrate that GlycosylationN - linked glycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.8950	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or GlycosylationO - linked glycosylation , contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.8607	Our findings demonstrate that N - linked glycosylation and Phosphorylationphosphorylation , but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.9494	Our findings demonstrate that N - linked Glycosylationglycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.9211	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or O - linked Glycosylationglycosylation , contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.7408	Our findings demonstrate that GlycosylationN - linked glycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.6591	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or GlycosylationO - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.

(603)

The modifications found are similar in the different mammalian expression systems explored.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(604)

Our findings indicate that the Semliki Forest virus system can provide large amounts of functional and fully glycosylated alpha ( 1B ) - AR protein suitable for biochemical and structural studies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9950	Our findings indicate that the Semliki Forest virus system can provide large amounts of functional and fully glycosylated Proteinalpha ( 1B ) - AR protein suitable for biochemical and structural studies.
0.9950	Our findings indicate that the Semliki Forest virus system can provide large amounts of functional and fully Glycosylationglycosylated alpha ( 1B ) - AR protein suitable for biochemical and structural studies.

(605)

The results of this study contribute to elucidate the basic steps involved in the processing of G protein - coupled receptors as well as to optimize strategies for their overexpression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(606)

Binding mode analysis of 3 - ( 4 - benzoyl - 1 - methyl - 1H - 2 - pyrrolyl ) - N - hydroxy - 2 - propenamide : a new synthetic histone deacetylase inhibitor inducing histone hyperacetylation, growth inhibition, and terminal cell differentiation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9960	Binding mode analysis of 3 - ( 4 - benzoyl - 1 - methyl - 1H - 2 - pyrrolyl ) - N - hydroxy - 2 - propenamide : a new synthetic Proteinhistone deacetylase inhibitor inducing histone hyperacetylation, growth inhibition, and terminal cell differentiation.
0.9941	Binding mode analysis of 3 - ( 4 - benzoyl - 1 - methyl - 1H - 2 - pyrrolyl ) - N - hydroxy - 2 - propenamide : a new synthetic histone deacetylase inhibitor inducing Proteinhistone hyperacetylation, growth inhibition, and terminal cell differentiation.
0.9219	Binding mode analysis of 3 - ( 4 - benzoyl - 1 - methyl - 1H - 2 - pyrrolyl ) - N - hydroxy - 2 - propenamide : a new synthetic histone deacetylase inhibitor inducing histone Acetylationhyperacetylation , growth inhibition, and terminal cell differentiation.

(607)

The binding mode of 3 - ( 4 - aroyl - 1H - 2 - pyrrolyl ) - N - hydroxy - 2 - propenamides 1a - c, belonging to a recently reported class of synthetic histone deacetylase ( HDAC ) inhibitors ( Massa, S. ; et al.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9816	The binding mode of 3 - ( 4 - aroyl - 1H - 2 - pyrrolyl ) - N - hydroxy - 2 - propenamides 1a - c, belonging to a recently reported class of synthetic Proteinhistone deacetylase ( HDAC ) inhibitors ( Massa, S. ; et al.

(608)

J.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(609)

Med.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(610)

Chem. 2001, 44, 2069 - 2072 ), into the new modeled HDAC1 catalytic core is presented, and enzyme / inhibitor interactions are discussed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Chem. 2001, 44, 2069 - 2072 ), into the new modeled ProteinHDAC1 catalytic core is presented, and enzyme / inhibitor interactions are discussed.

(611)

HDAC1 X - ray coordinates were obtained by virtual " mutation " of those of histone deacetylase - like protein, a bacterial HDAC homologue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	ProteinHDAC1 X - ray coordinates were obtained by virtual " mutation " of those of histone deacetylase - like protein, a bacterial HDAC homologue.
0.9798	HDAC1 X - ray coordinates were obtained by virtual " mutation " of those of Proteinhistone deacetylase - like protein, a bacterial HDAC homologue.

(612)

In in vitro antimaize HD2 as well as antimouse HDAC1 assay, compounds 1a - c showed inhibitory activities in the low micromolar range.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	In in vitro antimaize HD2 as well as antimouse ProteinHDAC1 assay, compounds 1a - c showed inhibitory activities in the low micromolar range.

(613)

Similarly, 1a - c are endowed with anti - HDAC activity in vivo : on mouse A20 cells, 1a - c induced histone hyperacetylation leading to a highly increased acetylation level of H4 as compared to control histones.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9962	Similarly, 1a - c are endowed with anti - HDAC activity in vivo : on mouse A20 cells, 1a - c induced histone hyperacetylation leading to a highly increased acetylation level of ProteinH4 as compared to control histones.
0.9948	Similarly, 1a - c are endowed with anti - HDAC activity in vivo : on mouse A20 cells, 1a - c induced Proteinhistone hyperacetylation leading to a highly increased acetylation level of H4 as compared to control histones.
0.9940	Similarly, 1a - c are endowed with anti - HDAC activity in vivo : on mouse A20 cells, 1a - c induced histone hyperacetylation leading to a highly increased Acetylationacetylation level of H4 as compared to control histones.
0.9826	Similarly, 1a - c are endowed with anti - HDAC activity in vivo : on mouse A20 cells, 1a - c induced histone Acetylationhyperacetylation leading to a highly increased acetylation level of H4 as compared to control histones.
0.9749	Similarly, 1a - c are endowed with anti - HDAC activity in vivo : on mouse A20 cells, 1a - c induced histone hyperacetylation leading to a highly increased acetylation level of H4 as compared to control Proteinhistones .

(614)

Results obtained with acid - urea - triton polyacrylamide gel electrophoresis have been confirmed by Western Blot experiments.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(615)

Finally, compound 1a, chosen as a representative member of this class of HDAC inhibitors, resulted endowed with antiproliferative ( 45 and 85 % cell growth inhibition at 40 and 80 microM, respectively ) and cellular differentiation ( 18 and 21 % of benzidine positive cells at the same concentrations ) activities in murine erythroleukemic cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(616)

Hypoxia - inducible factor ( HIF ) asparagine hydroxylase is identical to factor inhibiting HIF ( FIH ) and is related to the cupin structural family.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9874	Hypoxia - inducible factor ( HIF ) asparagine hydroxylase is identical to factor inhibiting HIF Protein( FIH ) and is related to the cupin structural family.
0.8119	Hypoxia - inducible factor ( HIF ) asparagine hydroxylase is identical to Proteinfactor inhibiting HIF ( FIH ) and is related to the cupin structural family.
	ProteinHypoxia - inducible factor ( HIF ) asparagine hydroxylase is identical to factor inhibiting HIF ( FIH ) and is related to the cupin structural family.

(617)

Activity of the hypoxia - inducible factor ( HIF ) complex is controlled by oxygen - dependent hydroxylation of prolyl and asparaginyl residues.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(618)

Hydroxylation of specific prolyl residues by 2 - oxoglutarate ( 2 - OG ) - dependent oxygenases mediates ubiquitinylation and proteasomal destruction of HIF - alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(619)

Hydroxylation of an asparagine residue in the C - terminal transactivation domain ( CAD ) of HIF - alpha abrogates interaction with p300, preventing transcriptional activation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9982	Hydroxylation of an asparagine residue in the C - terminal transactivation domain ( CAD ) of HIF - alpha abrogates interaction with Proteinp300 , preventing transcriptional activation.

(620)

Yeast two - hybrid assays recently identified factor inhibiting HIF ( FIH ) as a protein that associates with the CAD region of HIF - alpha.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9891	Yeast two - hybrid assays recently identified factor inhibiting HIF Protein( FIH ) as a protein that associates with the CAD region of HIF - alpha.
0.8293	Yeast two - hybrid assays recently identified Proteinfactor inhibiting HIF ( FIH ) as a protein that associates with the CAD region of HIF - alpha.
0.5683	Yeast two - hybrid assays recently identified factor Proteininhibiting HIF ( FIH ) as a protein that associates with the CAD region of HIF - alpha.

(621)

Since FIH contains certain motifs present in iron - and 2 - OG - dependent oxygenases we investigated whether FIH was the HIF asparaginyl hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Since FIH contains certain motifs present in iron - and 2 - OG - dependent oxygenases we investigated whether ProteinFIH was the HIF asparaginyl hydroxylase.
0.9996	Since ProteinFIH contains certain motifs present in iron - and 2 - OG - dependent oxygenases we investigated whether FIH was the HIF asparaginyl hydroxylase.
0.7733	Since FIH contains certain motifs present in iron - and 2 - OG - dependent oxygenases we investigated whether FIH was the ProteinHIF asparaginyl hydroxylase .

(622)

Assays using recombinant FIH and HIF - alpha fragments revealed that FIH is the enzyme that hydroxylates the CAD asparagine residue, that the activity is directly inhibited by cobalt ( II ) and limited by hypoxia, and that the oxygen in the alcohol of the hydroxyasparagine residue is directly derived from dioxygen.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9997	Assays using recombinant FIH and HIF - alpha fragments revealed that ProteinFIH is the enzyme that hydroxylates the CAD asparagine residue, that the activity is directly inhibited by cobalt ( II ) and limited by hypoxia, and that the oxygen in the alcohol of the hydroxyasparagine residue is directly derived from dioxygen.
0.9995	Assays using recombinant ProteinFIH and HIF - alpha fragments revealed that FIH is the enzyme that hydroxylates the CAD asparagine residue, that the activity is directly inhibited by cobalt ( II ) and limited by hypoxia, and that the oxygen in the alcohol of the hydroxyasparagine residue is directly derived from dioxygen.
0.8158	Assays using recombinant FIH and HIF - alpha fragments revealed that FIH is the enzyme that Hydroxylationhydroxylates the CAD asparagine residue, that the activity is directly inhibited by cobalt ( II ) and limited by hypoxia, and that the oxygen in the alcohol of the hydroxyasparagine residue is directly derived from dioxygen.

(623)

Sequence analyses involving FIH link the 2 - OG oxygenases with members of the cupin superfamily, including Zn ( II ) - utilizing phosphomannose isomerase, revealing structural and evolutionary links between these metal - binding proteins that share common motifs.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9999	Sequence analyses involving ProteinFIH link the 2 - OG oxygenases with members of the cupin superfamily, including Zn ( II ) - utilizing phosphomannose isomerase, revealing structural and evolutionary links between these metal - binding proteins that share common motifs.
0.9628	Sequence analyses involving FIH link the 2 - OG oxygenases with members of the cupin superfamily, including Zn ( II ) - utilizing Proteinphosphomannose isomerase , revealing structural and evolutionary links between these metal - binding proteins that share common motifs.
0.7164	Sequence analyses involving FIH link the 2 - OG oxygenases with members of the cupin superfamily, including ProteinZn ( II ) - utilizing phosphomannose isomerase , revealing structural and evolutionary links between these metal - binding proteins that share common motifs.

(624)

FIH - 1 is an asparaginyl hydroxylase enzyme that regulates the transcriptional activity of hypoxia - inducible factor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	ProteinFIH - 1 is an asparaginyl hydroxylase enzyme that regulates the transcriptional activity of hypoxia - inducible factor.

(625)

Mammalian cells adapt to hypoxic conditions through a transcriptional response pathway mediated by the hypoxia - inducible factor, HIF.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(626)

HIF transcriptional activity is suppressed under normoxic conditions by hydroxylation of an asparagine residue within its C - terminal transactivation domain, blocking association with coactivators.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.8306	HIF transcriptional activity is suppressed under normoxic conditions by Hydroxylationhydroxylation of an asparagine residue within its C - terminal transactivation domain, blocking association with coactivators.

(627)

Here we show that the protein FIH - 1, previously shown to interact with HIF, is an asparaginyl hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Here we show that the protein ProteinFIH - 1 , previously shown to interact with HIF, is an asparaginyl hydroxylase.

(628)

Like known hydroxylase enzymes, FIH - 1 is an Fe ( II ) - dependent enzyme that uses molecular O ( 2 ) to modify its substrate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	Like known hydroxylase enzymes, ProteinFIH - 1 is an Fe ( II ) - dependent enzyme that uses molecular O ( 2 ) to modify its substrate.

(629)

Together with the recently discovered prolyl hydroxylases that regulate HIF stability, this class of oxygen - dependent enzymes comprises critical regulatory components of the hypoxic response pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(630)

[ alpha ] - Secretase ADAM10 as well as [ alpha ] APPs is reduced in platelets and CSF of Alzheimer disease patients.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9787	[ alpha ] - Secretase ADAM10 as well as Protein[ alpha ] APPs is reduced in platelets and CSF of Alzheimer disease patients.
0.9684	[ alpha ] - Secretase ProteinADAM10 as well as [ alpha ] APPs is reduced in platelets and CSF of Alzheimer disease patients.

(631)

BACKGROUND : Members of membrane - bound disintegrin metalloproteinases ( ADAMs ) were shown to be capable of cleaving amyloid precursor protein ( APP ) at the alpha - cleavage site in different cell systems.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9161	BACKGROUND : Members of membrane - bound disintegrin metalloproteinases ( ADAMs ) were shown to be capable of cleaving amyloid precursor protein Protein( APP ) at the alpha - cleavage site in different cell systems.
0.8861	BACKGROUND : Members of membrane - bound disintegrin metalloproteinases ( ADAMs ) were shown to be capable of cleaving Proteinamyloid precursor protein ( APP ) at the alpha - cleavage site in different cell systems.

(632)

One of the candidate alpha - secretases identified in this family is ADAM10.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9979	One of the candidate alpha - secretases identified in this family is ProteinADAM10 .

(633)

The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ADAM10 levels in the peripheral cells of AD patients related to a concomitant decrease in alpha APPs? MATERIALS AND METHODS : Western Blot analysis of ADAM10 is performed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.

Precision: 30.00

Recall: 75.00

F1: 42.86

Score	Text
0.9986	The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ProteinADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ADAM10 levels in the peripheral cells of AD patients related to a concomitant decrease in alpha APPs? MATERIALS AND METHODS : Western Blot analysis of ADAM10 is performed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.
0.9234	The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ADAM10 levels in the peripheral cells of AD patients related to a concomitant decrease in alpha APPs? MATERIALS AND METHODS : Western Blot analysis of ADAM10 is Proteinperformed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.
0.5594	The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ADAM10 levels in the peripheral cells of AD patients related to a concomitant decrease in alpha ProteinAPPs? MATERIALS AND METHODS : Western Blot analysis of ADAM10 is performed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.
0.9990	The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ADAM10 levels in the peripheral cells of AD patients related to a concomitant decrease in alpha APPs? MATERIALS AND METHODS : Western Blot analysis of ProteinADAM10 is performed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.
0.9989	The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ProteinADAM10 levels in the peripheral cells of AD patients related to a concomitant decrease in alpha APPs? MATERIALS AND METHODS : Western Blot analysis of ADAM10 is performed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.
0.6958	The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ADAM10 levels in the peripheral cells of AD patients related to a concomitant decrease in alpha APPs? ProteinMATERIALS AND METHODS : Western Blot analysis of ADAM10 is performed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.
0.6089	The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ADAM10 levels in the peripheral cells of AD patients related to a concomitant decrease in Proteinalpha APPs? MATERIALS AND METHODS : Western Blot analysis of ADAM10 is performed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.
0.6018	The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ADAM10 levels in the peripheral cells of AD patients related to a concomitant decrease in Proteinalpha APPs? MATERIALS AND METHODS : Western Blot analysis of ADAM10 is performed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.
0.5532	The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ProteinADAM10 levels in the peripheral cells of AD patients related to a concomitant decrease in alpha APPs? MATERIALS AND METHODS : Western Blot analysis of ADAM10 is performed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.
0.5130	The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ADAM10 levels in the peripheral cells of AD patients related to a concomitant decrease in Proteinalpha APPs? MATERIALS AND METHODS : Western Blot analysis of ADAM10 is performed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.
	The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ADAM10 Proteinlevels in the peripheral cells of AD patients related to a concomitant decrease in alpha APPs? MATERIALS AND METHODS : Western Blot analysis of ADAM10 is performed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.

(634)

Moreover, the levels of alpha - secretase metabolite ( alpha APPs ) are tested both in platelets and cerebrospinal fluid ( CSF ) of the same pool of subjects by means of Western blot with a specific antibody.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Text
0.8160	Moreover, the levels of alpha - secretase metabolite Protein( alpha APPs ) are tested both in platelets and cerebrospinal fluid ( CSF ) of the same pool of subjects by means of Western blot with a specific antibody.
0.8325	Moreover, the levels of alpha - secretase metabolite ( alpha ProteinAPPs ) are tested both in platelets and cerebrospinal fluid ( CSF ) of the same pool of subjects by means of Western blot with a specific antibody.
0.5920	Moreover, the levels of alpha - secretase metabolite Protein( alpha APPs ) are tested both in platelets and cerebrospinal fluid ( CSF ) of the same pool of subjects by means of Western blot with a specific antibody.

(635)

RESULTS : A significant decrease of platelet ADAM10 levels is observed in patients affected by probable AD when compared to control subjects and this is paralleled by a reduced level of alpha APPs released from platelets.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9984	RESULTS : A significant decrease of platelet ProteinADAM10 levels is observed in patients affected by probable AD when compared to control subjects and this is paralleled by a reduced level of alpha APPs released from platelets.
0.9784	RESULTS : A significant decrease of platelet ADAM10 levels is observed in patients affected by probable AD when compared to control subjects and this is paralleled by a reduced level of Proteinalpha APPs released from platelets.
0.5995	RESULTS : A significant decrease of platelet ADAM10 levels is observed in patients affected by probable AD when compared to control subjects and this is paralleled by a reduced level of alpha ProteinAPPs released from platelets.

(636)

Moreover, in the same pool of AD patients, alpha APPs levels were reduced concomitantly in CSF.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9375	Moreover, in the same pool of AD patients, Proteinalpha APPs levels were reduced concomitantly in CSF.

(637)

CONCLUSIONS : ADAM10 is expressed in platelets.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	CONCLUSIONS : ProteinADAM10 is expressed in platelets.

(638)

A reduced level of ADAM10 is observed in platelets obtained from AD patients compared to age - matched controls.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	A reduced level of ProteinADAM10 is observed in platelets obtained from AD patients compared to age - matched controls.

(639)

Further, in the same pool of AD patients, a qualitatively and quantitatively similar decrease in alpha APPs is present both in thrombin - activated platelets and CSF, thus suggesting that alterations of APP processing might occur both in the neuronal compartment and peripheral cells.

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Text
0.9992	Further, in the same pool of AD patients, a qualitatively and quantitatively similar decrease in alpha APPs is present both in Proteinthrombin - activated platelets and CSF, thus suggesting that alterations of APP processing might occur both in the neuronal compartment and peripheral cells.
0.9955	Further, in the same pool of AD patients, a qualitatively and quantitatively similar decrease in alpha APPs is present both in thrombin - activated platelets and CSF, thus suggesting that alterations of ProteinAPP processing might occur both in the neuronal compartment and peripheral cells.
0.9944	Further, in the same pool of AD patients, a qualitatively and quantitatively similar decrease in Proteinalpha APPs is present both in thrombin - activated platelets and CSF, thus suggesting that alterations of APP processing might occur both in the neuronal compartment and peripheral cells.
0.9610	Further, in the same pool of AD patients, a qualitatively and quantitatively similar decrease in alpha ProteinAPPs is present both in thrombin - activated platelets and CSF, thus suggesting that alterations of APP processing might occur both in the neuronal compartment and peripheral cells.
0.5273	Further, in the same pool of AD patients, a qualitatively and quantitatively similar decrease in Proteinalpha APPs is present both in thrombin - activated platelets and CSF, thus suggesting that alterations of APP processing might occur both in the neuronal compartment and peripheral cells.

(640)

Reduced expression of the insulin - induced protein 1 and p41 Arp2 / 3 complex genes in human gastric cancers.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9636	Reduced expression of the insulin - induced protein 1 and Proteinp41 Arp2 / 3 complex genes in human gastric cancers.
0.9342	Reduced expression of the Proteininsulin - induced protein 1 and p41 Arp2 / 3 complex genes in human gastric cancers.
	Reduced expression of the insulin - induced protein 1 and p41 ProteinArp2 / 3 complex genes in human gastric cancers.

(641)

Aberrantly methylated DNA fragments in a human gastric cancer were searched for by a genome - scanning method, methylation - sensitive - representational difference analysis ( MS - RDA ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(642)

Six DNA fragments flanked by CpG islands ( CGIs ) and hypermethylated in the cancer were isolated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(643)

Four of the 6 fragments possessed genes in their vicinities.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(644)

Quantitative RT - PCR analysis of the 4 genes showed reduced expression of 2 genes in cancers : Insulin - induced protein 1 ( INSIG1 / CL - 6 ) and p41 Arp2 / 3 complex ( p41 - Arc ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9857	Quantitative RT - PCR analysis of the 4 genes showed reduced expression of 2 genes in cancers : Insulin - induced protein 1 ( INSIG1 / CL - 6 ) and p41 Arp2 / 3 complex Protein( p41 - Arc ) .
0.9848	Quantitative RT - PCR analysis of the 4 genes showed reduced expression of 2 genes in cancers : Insulin - induced protein 1 Protein( INSIG1 / CL - 6 ) and p41 Arp2 / 3 complex ( p41 - Arc ).
0.9452	Quantitative RT - PCR analysis of the 4 genes showed reduced expression of 2 genes in cancers : Insulin - induced protein 1 ( INSIG1 / CL - 6 ) and Proteinp41 Arp2 / 3 complex ( p41 - Arc ).
0.8389	Quantitative RT - PCR analysis of the 4 genes showed reduced expression of 2 genes in cancers : ProteinInsulin - induced protein 1 ( INSIG1 / CL - 6 ) and p41 Arp2 / 3 complex ( p41 - Arc ).

(645)

As for INSIG1, a DNA fragment was derived from the edge of a CGI in the promoter region.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	As for ProteinINSIG1 , a DNA fragment was derived from the edge of a CGI in the promoter region.

(646)

The edge was methylated in 11 of 22 primary gastric cancers, whereas the center was not methylated in any cancer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9918	The edge was methylated in 11 of 22 primary gastric cancers, whereas the center was not DNA_methylationmethylated in any cancer.
0.9909	The edge was DNA_methylationmethylated in 11 of 22 primary gastric cancers, whereas the center was not methylated in any cancer.
0.9346	The edge was methylated in 11 of 22 primary gastric cancers, whereas the Entitycenter was not methylated in any cancer.
0.9077	The Entityedge was methylated in 11 of 22 primary gastric cancers, whereas the center was not methylated in any cancer.

(647)

INSIG1 expression was markedly reduced in 19 cancers, including the 11 cancers with the methylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9984	ProteinINSIG1 expression was markedly reduced in 19 cancers, including the 11 cancers with the methylation.
0.9950	INSIG1 expression was markedly reduced in 19 cancers, including the 11 cancers with the DNA_methylationmethylation .

(648)

By 5 - aza - 2'- deoxycytidine treatment of 5 cell lines with the methylation of the edge, partial restoration of INSIG1 expression was detected only in 2 of them.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	By 5 - aza - 2'- deoxycytidine treatment of 5 cell lines with the methylation of the edge, partial restoration of ProteinINSIG1 expression was detected only in 2 of them.
0.9880	By 5 - aza - 2'- deoxycytidine treatment of 5 cell lines with the methylation of the Entityedge , partial restoration of INSIG1 expression was detected only in 2 of them.
0.9879	By 5 - aza - 2'- deoxycytidine treatment of 5 cell lines with the DNA_methylationmethylation of the edge, partial restoration of INSIG1 expression was detected only in 2 of them.

(649)

These data indicated that, although the reduced INSIG1 expression in cancers was associated with the methylation at the edge of the CGI in the promoter region, the methylation was likely to be a secondary change.

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Text
0.9971	These data indicated that, although the reduced ProteinINSIG1 expression in cancers was associated with the methylation at the edge of the CGI in the promoter region, the methylation was likely to be a secondary change.
0.9885	These data indicated that, although the reduced INSIG1 expression in cancers was associated with the DNA_methylationmethylation at the edge of the CGI in the promoter region, the methylation was likely to be a secondary change.
0.8987	These data indicated that, although the reduced INSIG1 expression in cancers was associated with the methylation at the Entityedge of the CGI in the promoter region, the methylation was likely to be a secondary change.
0.9885	These data indicated that, although the reduced INSIG1 expression in cancers was associated with the methylation at the edge of the CGI in the promoter region, the DNA_methylationmethylation was likely to be a secondary change.
0.9677	These data indicated that, although the reduced INSIG1 expression in cancers was associated with the methylation at the edge of the EntityCGI in the promoter region, the methylation was likely to be a secondary change.

(650)

As for p41 - Arc, a DNA fragment was derived from a CGI overlapping exon 8, and its methylation did not correlate with its expression.

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Text
0.9988	As for Proteinp41 - Arc , a DNA fragment was derived from a CGI overlapping exon 8, and its methylation did not correlate with its expression.
0.9527	As for p41 - Arc, a DNA fragment was derived from a CGI overlapping exon 8, and its DNA_methylationmethylation did not correlate with its expression.
0.7971	As for p41 - Arc, a DNA fragment was derived from a EntityCGI overlapping exon 8 , and its methylation did not correlate with its expression.
0.8021	As for p41 - Arc, a DNA fragment was derived from a CGI overlapping Entityexon 8 , and its methylation did not correlate with its expression.
0.7886	As for p41 - Arc, a DNA fragment was derived from a CGI Entityoverlapping exon 8 , and its methylation did not correlate with its expression.

(651)

However, methylation of a CGI in the promoter region with a marked reduction of its expression was observed in 1 of the 22 primary cancers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9939	However, DNA_methylationmethylation of a CGI in the promoter region with a marked reduction of its expression was observed in 1 of the 22 primary cancers.
0.9069	However, methylation of a EntityCGI in the promoter region with a marked reduction of its expression was observed in 1 of the 22 primary cancers.

(652)

INSIG1 and p41 - Arc are known to be involved in cellular differentiation and morphology, respectively, and it was suggested that their reduced expressions might be involved in gastric cancer development or progression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	INSIG1 and Proteinp41 - Arc are known to be involved in cellular differentiation and morphology, respectively, and it was suggested that their reduced expressions might be involved in gastric cancer development or progression.
0.9998	ProteinINSIG1 and p41 - Arc are known to be involved in cellular differentiation and morphology, respectively, and it was suggested that their reduced expressions might be involved in gastric cancer development or progression.

(653)

The kyphoscoliotic type of Ehlers - Danlos syndrome ( type VI ) : differential effects on the hydroxylation of lysine in collagens I and II revealed by analysis of cross - linked telopeptides from urine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9817	The kyphoscoliotic type of Ehlers - Danlos syndrome ( type VI ) : differential effects on the hydroxylation of lysine in collagens I and ProteinII revealed by analysis of cross - linked telopeptides from urine.
0.9813	The kyphoscoliotic type of Ehlers - Danlos syndrome ( type VI ) : differential effects on the Hydroxylationhydroxylation of lysine in collagens I and II revealed by analysis of cross - linked telopeptides from urine.
0.9658	The kyphoscoliotic type of Ehlers - Danlos syndrome ( type VI ) : differential effects on the hydroxylation of Entitylysine in collagens I and II revealed by analysis of cross - linked telopeptides from urine.

(654)

The kyphoscoliotic type of Ehlers - Danlos syndrome ( EDS type VIA ) ( OMIM 225400 ) is an autosomal recessive connective tissue disorder that results from mutations in the lysyl hydroxylase 1 gene ( PLOD1 ) causing underhydroxylation of lysine residues in tissue collagens, particularly of skin.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9796	The kyphoscoliotic type of Ehlers - Danlos syndrome ( EDS type VIA ) ( OMIM 225400 ) is an autosomal recessive connective tissue disorder that results from mutations in the lysyl hydroxylase 1 gene Protein( PLOD1 ) causing underhydroxylation of lysine residues in tissue collagens, particularly of skin.
0.7973	The kyphoscoliotic type of Ehlers - Danlos syndrome ( EDS type VIA ) ( OMIM 225400 ) is an autosomal recessive connective tissue disorder that results from mutations in the Proteinlysyl hydroxylase 1 gene ( PLOD1 ) causing underhydroxylation of lysine residues in tissue collagens, particularly of skin.
0.5736	The kyphoscoliotic type of Ehlers - Danlos syndrome ( EDS type VIA ) ( OMIM 225400 ) is an autosomal recessive connective tissue disorder that results from mutations in the lysyl hydroxylase 1 gene ( PLOD1 ) causing underhydroxylation of Entitylysine residues in tissue collagens, particularly of skin.

(655)

Previous studies have shown that the pool of collagen cross - linking amino acids, hydroxylysyl pyridinoline ( HP ) and lysyl pyridinoline ( LP ) excreted in urine has an abnormally low HP / LP ratio, which is diagnostic of the condition.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(656)

Here we isolated cross - linked peptides containing these residues from the urine of a child with EDS VIA homozygous for a mutation that results in a stop codon and effective null expression of PLOD1 enzyme activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Here we isolated cross - linked peptides containing these residues from the urine of a child with EDS VIA homozygous for a mutation that results in a stop codon and effective null expression of ProteinPLOD1 enzyme activity.

(657)

Peptides that had originated from bone type I collagen and cartilage type II collagen were identified.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.8610	Peptides that had originated from bone type I collagen and cartilage Proteintype II collagen were identified.

(658)

A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical lysines at residues 930 of alpha 1 ( I ) and 933 of alpha 2 ( I ) of the collagen triple - helix had not been hydroxylated.

Precision: 62.50

Recall: 100.00

F1: 76.92

Score	Text
0.9621	A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical lysines at residues 930 of alpha 1 ( I ) and Entity933 of alpha 2 ( I ) of the collagen triple - helix had not been hydroxylated.
0.9561	A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical lysines at residues 930 of alpha 1 ( I ) and 933 of alpha 2 ( I ) of the collagen triple - helix had not been Hydroxylationhydroxylated .
0.9348	A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical lysines at residues 930 of alpha 1 ( I ) and 933 of Proteinalpha 2 ( I ) of the collagen triple - helix had not been hydroxylated.
0.9304	A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical lysines at residues 930 of Proteinalpha 1 ( I ) and 933 of alpha 2 ( I ) of the collagen triple - helix had not been hydroxylated.
0.8707	A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical lysines at Entityresidues 930 of alpha 1 ( I ) and 933 of alpha 2 ( I ) of the collagen triple - helix had not been hydroxylated.
0.9294	A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical lysines at residues Entity930 of alpha 1 ( I ) and 933 of alpha 2 ( I ) of the collagen triple - helix had not been hydroxylated.
0.8101	A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical Entitylysines at residues 930 of alpha 1 ( I ) and 933 of alpha 2 ( I ) of the collagen triple - helix had not been hydroxylated.
0.6139	A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical lysines at residues 930 of alpha 1 ( I ) and 933 of alpha Protein2 ( I ) of the collagen triple - helix had not been hydroxylated.

(659)

The equivalent peptide fraction from a normal child's urine gave a ratio of HP to LP of 1. 5 : 1 typical for normal bone collagen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(660)

A second cross - linked peptide that is derived from the C - telopeptide domain of cartilage type II collagen showed both HP and LP in a 2 : 1 ratio, compared with 18 : 1 for the equivalent peptide from a normal child's urine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9532	A second cross - linked peptide that is derived from the C - telopeptide domain of cartilage Proteintype II collagen showed both HP and LP in a 2 : 1 ratio, compared with 18 : 1 for the equivalent peptide from a normal child's urine.

(661)

The results show that in EDS VIA, bone type I collagen is more markedly underhydroxylated than cartilage type II collagen, at least at those helical sites that form cross - links.

Precision: 50.00

Recall: 50.00

F1: 50.00

Score	Text
0.9299	The results show that in EDS VIA, bone type I collagen is more markedly underhydroxylated than cartilage Proteintype II collagen , at least at those helical sites that form cross - links.
0.2504	The results show that in EDS VIA, bone type I collagen is more markedly Glycosylationunderhydroxylated than cartilage type II collagen, at least at those helical sites that form cross - links.
	The results show that in EDS VIA, bone type I collagen is more markedly Hydroxylationunderhydroxylated than cartilage type II collagen, at least at those helical sites that form cross - links.

(662)

The residual fraction of HP found in the urine of EDS VI patients therefore appears to be contributed in significant part by the degradation products of cartilage.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(663)

Since PLOD1 is null, other PLOD genes must be responsible for the helical hydroxylation activity that results in HP.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	Since ProteinPLOD1 is null, other PLOD genes must be responsible for the helical hydroxylation activity that results in HP.

(664)

The presented approach of analyzing urinary cross - linked C - telopeptide fragments of type II collagen may allow the detection of chondrodysplasias due to genetic defects in lysyl hydroxylase isoforms active in cartilage.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9348	The presented approach of analyzing urinary cross - linked C - telopeptide fragments of Proteintype II collagen may allow the detection of chondrodysplasias due to genetic defects in lysyl hydroxylase isoforms active in cartilage.

(665)

Postsynthetic trimethylation of histone H4 at lysine 20 in mammalian tissues is associated with aging.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9843	Postsynthetic Methylationtrimethylation of histone H4 at lysine 20 in mammalian tissues is associated with aging.
0.8944	Postsynthetic trimethylation of Proteinhistone H4 at lysine 20 in mammalian tissues is associated with aging.
0.8836	Postsynthetic trimethylation of histone H4 at Entitylysine 20 in mammalian tissues is associated with aging.

(666)

Methylation of the N - terminal region of histones was first described more than 35 years ago, but its biological significance has remained unclear.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9641	Methylation of the N - terminal region of Proteinhistones was first described more than 35 years ago, but its biological significance has remained unclear.
0.8936	Methylation of the EntityN - terminal region of histones was first described more than 35 years ago, but its biological significance has remained unclear.
0.8667	MethylationMethylation of the N - terminal region of histones was first described more than 35 years ago, but its biological significance has remained unclear.
0.8506	Methylation of the EntityN - terminal region of histones was first described more than 35 years ago, but its biological significance has remained unclear.

(667)

Proposed functions range from transcriptional regulation to the higher order packing of chromatin in progress of mitotic condensation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(668)

Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases SUV39H1 and Suv39h1, which selectively methylate lysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.

Precision: 77.78

Recall: 87.50

F1: 82.35

Score	Text
0.9948	Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases SUV39H1 and ProteinSuv39h1 , which selectively methylate lysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.
0.9940	Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases ProteinSUV39H1 and Suv39h1, which selectively methylate lysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.
0.9753	Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases SUV39H1 and Suv39h1, which selectively methylate lysine 9 of the ProteinH3 N terminus, this posttranslational modification has regained scientific interest.
0.9666	Primarily because of the recent discovery of the SET domain - depending ProteinH3 - specific histone methyltransferases SUV39H1 and Suv39h1, which selectively methylate lysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.
0.9609	Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases SUV39H1 and Suv39h1, which selectively methylate Entitylysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.
0.9606	Primarily because of the recent discovery of the SET domain - depending H3 - specific Proteinhistone methyltransferases SUV39H1 and Suv39h1, which selectively methylate lysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.
0.5045	Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases SUV39H1 and Suv39h1, which selectively Methylationmethylate lysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.
0.9751	Primarily because of the recent discovery of the SET domain - depending ProteinH3 - specific histone methyltransferases SUV39H1 and Suv39h1, which selectively methylate lysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.
0.7632	Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases SUV39H1 and Suv39h1, which selectively methylate Entitylysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.
	Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases SUV39H1 and Suv39h1, which selectively Catalysismethylate lysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.

(669)

In the past, investigations concerning the biological significance of histone methylation were largely limited because of a lack of simple and sensitive analytical procedures for detecting this modification.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9890	In the past, investigations concerning the biological significance of histone Methylationmethylation were largely limited because of a lack of simple and sensitive analytical procedures for detecting this modification.
0.9868	In the past, investigations concerning the biological significance of Proteinhistone methylation were largely limited because of a lack of simple and sensitive analytical procedures for detecting this modification.

(670)

The present work investigated the methylation pattern of histone H4 both in different mammalian organs of various ages and in cell lines by applying mass spectrometric analysis and a newly developed hydrophilic - interaction liquid chromatographic method enabling the simultaneous separation of methylated and acetylated forms, which obviates the need to work with radioactive materials.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9893	The present work investigated the Methylationmethylation pattern of histone H4 both in different mammalian organs of various ages and in cell lines by applying mass spectrometric analysis and a newly developed hydrophilic - interaction liquid chromatographic method enabling the simultaneous separation of methylated and acetylated forms, which obviates the need to work with radioactive materials.
0.9753	The present work investigated the methylation pattern of Proteinhistone H4 both in different mammalian organs of various ages and in cell lines by applying mass spectrometric analysis and a newly developed hydrophilic - interaction liquid chromatographic method enabling the simultaneous separation of methylated and acetylated forms, which obviates the need to work with radioactive materials.
0.7084	The present work investigated the methylation pattern of histone H4 both in different mammalian organs of various ages and in cell lines by applying mass spectrometric analysis and a newly developed hydrophilic - interaction liquid chromatographic method enabling the simultaneous separation of Methylationmethylated and acetylated forms, which obviates the need to work with radioactive materials.
0.7025	The present work investigated the methylation pattern of histone H4 both in different mammalian organs of various ages and in cell lines by applying mass spectrometric analysis and a newly developed hydrophilic - interaction liquid chromatographic method enabling the simultaneous separation of methylated and Acetylationacetylated forms, which obviates the need to work with radioactive materials.

(671)

In rat kidney and liver the dimethylated lysine 20 was found to be the main methylation product, whereas the monomethyl derivative was present in much smaller amounts.

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Text
0.9705	In rat kidney and liver the Methylationdimethylated lysine 20 was found to be the main methylation product, whereas the monomethyl derivative was present in much smaller amounts.
0.9286	In rat kidney and liver the dimethylated lysine 20 was found to be the main methylation product, whereas the Methylationmonomethyl derivative was present in much smaller amounts.
0.9131	In rat kidney and liver the dimethylated Entitylysine 20 was found to be the main methylation product, whereas the monomethyl derivative was present in much smaller amounts.
0.6436	In rat kidney and liver the dimethylated Entitylysine 20 was found to be the main methylation product, whereas the monomethyl derivative was present in much smaller amounts.
0.5199	In rat kidney and liver the dimethylated lysine 20 was found to be the main Methylationmethylation product, whereas the monomethyl derivative was present in much smaller amounts.

(672)

In addition, for the first time a trimethylated form of lysine 20 of H4 was found in mammalian tissue.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9797	In addition, for the first time a Methylationtrimethylated form of lysine 20 of H4 was found in mammalian tissue.
0.9664	In addition, for the first time a trimethylated form of lysine 20 of ProteinH4 was found in mammalian tissue.
0.9549	In addition, for the first time a trimethylated form of Entitylysine 20 of H4 was found in mammalian tissue.
0.6508	In addition, for the first time a trimethylated form of Entitylysine 20 of H4 was found in mammalian tissue.

(673)

A significant increase in this trimethylated histone H4 was detected in organs of animals older than 30 days, whereas the amounts of mono - and dimethylated forms did not essentially change in organs from young ( 10 days old ) or old animals ( 30 and 450 days old ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9849	A significant increase in this Methylationtrimethylated histone H4 was detected in organs of animals older than 30 days, whereas the amounts of mono - and dimethylated forms did not essentially change in organs from young ( 10 days old ) or old animals ( 30 and 450 days old ).
0.9770	A significant increase in this trimethylated histone H4 was detected in organs of animals older than 30 days, whereas the amounts of mono - and Methylationdimethylated forms did not essentially change in organs from young ( 10 days old ) or old animals ( 30 and 450 days old ).
0.9747	A significant increase in this trimethylated histone H4 was detected in organs of animals older than 30 days, whereas the amounts of Methylationmono - and dimethylated forms did not essentially change in organs from young ( 10 days old ) or old animals ( 30 and 450 days old ).
0.9511	A significant increase in this trimethylated Proteinhistone H4 was detected in organs of animals older than 30 days, whereas the amounts of mono - and dimethylated forms did not essentially change in organs from young ( 10 days old ) or old animals ( 30 and 450 days old ).

(674)

Trimethylated H4 was also detected in transformed cells ; although it was present in only trace amounts in logarithmically growing cells, we found an increase in trimethylated lysine 20 in cells in the stationary phase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9964	Trimethylated ProteinH4 was also detected in transformed cells ; although it was present in only trace amounts in logarithmically growing cells, we found an increase in trimethylated lysine 20 in cells in the stationary phase.
0.9951	Trimethylated H4 was also detected in transformed cells ; although it was present in only trace amounts in logarithmically growing cells, we found an increase in Methylationtrimethylated lysine 20 in cells in the stationary phase.
0.9903	MethylationTrimethylated H4 was also detected in transformed cells ; although it was present in only trace amounts in logarithmically growing cells, we found an increase in trimethylated lysine 20 in cells in the stationary phase.
0.9192	Trimethylated H4 was also detected in transformed cells ; although it was present in only trace amounts in logarithmically growing cells, we found an increase in trimethylated Entitylysine 20 in cells in the stationary phase.

(675)

In the cystathionine beta - synthase knockout mouse, elevations in total plasma homocysteine increase tissue S - adenosylhomocysteine, but responses of S - adenosylmethionine and DNA methylation are tissue specific.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9734	In the Proteincystathionine beta - synthase knockout mouse, elevations in total plasma homocysteine increase tissue S - adenosylhomocysteine, but responses of S - adenosylmethionine and DNA methylation are tissue specific.

(676)

The cystathionine beta - synthase knockout mouse provides a unique opportunity to study biochemical consequences of a defective cystathionine beta - synthase enzyme.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9700	The Proteincystathionine beta - synthase knockout mouse provides a unique opportunity to study biochemical consequences of a defective cystathionine beta - synthase enzyme.
0.9385	The cystathionine beta - synthase knockout mouse provides a unique opportunity to study biochemical consequences of a defective Proteincystathionine beta - synthase enzyme.

(677)

The present study was undertaken to assess the effect of elevated plasma total homocysteine caused by cystathionine beta - synthase deficiency on one - carbon metabolism in 10 homozygous mutant mice and 10 age - and sex - matched wild - type mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9727	The present study was undertaken to assess the effect of elevated plasma total homocysteine caused by Proteincystathionine beta - synthase deficiency on one - carbon metabolism in 10 homozygous mutant mice and 10 age - and sex - matched wild - type mice.

(678)

Plasma total homocysteine levels, S - adenosylmethionine and S - adenosylhomocysteine concentrations in liver, kidney and brain were measured by HPLC.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(679)

Tissue DNA methylation status was measured by in vitro DNA methyl acceptance.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(680)

Plasma total homocysteine concentration in food - deprived homozygous mutant mice ( 271. 1 + / - 61. 5 micro mol / L ) was markedly higher than in wild - type mice ( 7. 4 + / - 2. 9 micro mol / L ) ( P < 0. 001 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(681)

In liver only, S - adenosylmethionine concentrations were higher in the homozygous mutant mice ( 35. 6 + / - 5. 9 nmol / g ) than in wild type mice ( 19. 1 + / - 6. 1 nmol / g ) ( P < 0. 001 ) and tended to be lower in kidney ( P = 0. 07 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(682)

In contrast, S - adenosylhomocysteine concentrations were significantly higher in homozygous mutant mice compared with wild - type mice in all tissues studied.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(683)

Genomic DNA methylation status in homozygous mutant compared with wild - type mice was lower in liver ( P = 0. 037 ) and tended to be lower in kidney ( P = 0. 077 ) but did not differ in brain ( P = 0. 46 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(684)

The results of this study are consistent with the predicted role of cystathionine beta - synthase in the regulation of plasma total homocysteine levels and tissue S - adenosylhomocysteine levels.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9716	The results of this study are consistent with the predicted role of Proteincystathionine beta - synthase in the regulation of plasma total homocysteine levels and tissue S - adenosylhomocysteine levels.

(685)

However, the fact that the absence of the enzyme had differential effects on S - adenosylmethionine concentrations and DNA methylation status in different tissues suggests that regulation of biological methylation is a complex tissue - specific phenomenon.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(686)

Glycosylation of human proteinase - activated receptor - 2 ( hPAR2 ) : role in cell surface expression and signalling.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9720	GlycosylationGlycosylation of human proteinase - activated receptor - 2 ( hPAR2 ) : role in cell surface expression and signalling.
0.9486	Glycosylation of human proteinase - activated receptor - 2 Protein( hPAR2 ) : role in cell surface expression and signalling.
0.6816	Glycosylation of human Proteinproteinase - activated receptor - 2 ( hPAR2 ) : role in cell surface expression and signalling.

(687)

We have analysed the role of N - linked glycosylation in regulating human proteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Text
0.9764	We have analysed the role of N - linked glycosylation in regulating human proteinase - activated receptor - 2 Protein( hPAR ( 2 ) ) expression and function.
0.9109	We have analysed the role of GlycosylationN - linked glycosylation in regulating human proteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.
0.7643	We have analysed the role of N - linked glycosylation in regulating human Proteinproteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.
0.9603	We have analysed the role of N - linked Glycosylationglycosylation in regulating human proteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.
0.5297	We have analysed the role of GlycosylationN - linked glycosylation in regulating human proteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.

(688)

Epitope - tagged wild - type hPAR ( 2 ) ( wt - hPAR ( 2 ) ) or hPAR ( 2 ) that lacked glycosylation sequons ( following site - directed mutagenesis ) in either the N - terminus [ hPAR ( 2 ) N30A ( Asn ( 30 ) - - > Ala ) ], extracellular loop 2 [ ECL2 ; hPAR ( 2 ) N222Q ( Asn ( 222 ) - - > Gln ) or hPAR ( 2 ) N222A ( Asn ( 222 ) - - > Ala ) ] or both ( hPAR ( 2 ) N30A, N222A or hPAR ( 2 ) N30A, N222Q ) were expressed in the Chinese - hamster ovary ( CHO ) fibroblast cell line, Pro5.

Precision: 88.89

Recall: 100.00

F1: 94.12

Score	Text
0.9991	Epitope - tagged wild - type hPAR ( 2 ) ( wt - hPAR ( 2 ) ) or ProteinhPAR ( 2 ) that lacked glycosylation sequons ( following site - directed mutagenesis ) in either the N - terminus [ hPAR ( 2 ) N30A ( Asn ( 30 ) - - > Ala ) ], extracellular loop 2 [ ECL2 ; hPAR ( 2 ) N222Q ( Asn ( 222 ) - - > Gln ) or hPAR ( 2 ) N222A ( Asn ( 222 ) - - > Ala ) ] or both ( hPAR ( 2 ) N30A, N222A or hPAR ( 2 ) N30A, N222Q ) were expressed in the Chinese - hamster ovary ( CHO ) fibroblast cell line, Pro5.
0.9989	Epitope - tagged wild - type ProteinhPAR ( 2 ) ( wt - hPAR ( 2 ) ) or hPAR ( 2 ) that lacked glycosylation sequons ( following site - directed mutagenesis ) in either the N - terminus [ hPAR ( 2 ) N30A ( Asn ( 30 ) - - > Ala ) ], extracellular loop 2 [ ECL2 ; hPAR ( 2 ) N222Q ( Asn ( 222 ) - - > Gln ) or hPAR ( 2 ) N222A ( Asn ( 222 ) - - > Ala ) ] or both ( hPAR ( 2 ) N30A, N222A or hPAR ( 2 ) N30A, N222Q ) were expressed in the Chinese - hamster ovary ( CHO ) fibroblast cell line, Pro5.
0.9968	Epitope - tagged wild - type hPAR ( 2 ) Protein( wt - hPAR ( 2 ) ) or hPAR ( 2 ) that lacked glycosylation sequons ( following site - directed mutagenesis ) in either the N - terminus [ hPAR ( 2 ) N30A ( Asn ( 30 ) - - > Ala ) ], extracellular loop 2 [ ECL2 ; hPAR ( 2 ) N222Q ( Asn ( 222 ) - - > Gln ) or hPAR ( 2 ) N222A ( Asn ( 222 ) - - > Ala ) ] or both ( hPAR ( 2 ) N30A, N222A or hPAR ( 2 ) N30A, N222Q ) were expressed in the Chinese - hamster ovary ( CHO ) fibroblast cell line, Pro5.
0.9940	Epitope - tagged wild - type hPAR ( 2 ) ( wt - hPAR ( 2 ) ) or hPAR ( 2 ) that lacked glycosylation sequons ( following site - directed mutagenesis ) in either the N - terminus [ hPAR ( 2 ) N30A ( Asn ( 30 ) - - > Ala ) ], extracellular loop 2 [ ECL2 ; hPAR ( 2 ) N222Q ( Asn ( 222 ) - - > Gln ) or hPAR ( 2 ) N222A ( Asn ( 222 ) - - > Ala ) ] or both ( hPAR ( 2 ) N30A, N222A or ProteinhPAR ( 2 ) N30A , N222Q ) were expressed in the Chinese - hamster ovary ( CHO ) fibroblast cell line, Pro5.
0.9907	Epitope - tagged wild - type hPAR ( 2 ) ( wt - hPAR ( 2 ) ) or hPAR ( 2 ) that lacked glycosylation sequons ( following site - directed mutagenesis ) in either the N - terminus [ hPAR ( 2 ) N30A ( Asn ( 30 ) - - > Ala ) ], extracellular loop 2 [ ECL2 ; hPAR ( 2 ) N222Q ( Asn ( 222 ) - - > Gln ) or hPAR ( 2 ) N222A ( Asn ( 222 ) - - > Ala ) ] or both Protein( hPAR ( 2 ) N30A , N222A or hPAR ( 2 ) N30A, N222Q ) were expressed in the Chinese - hamster ovary ( CHO ) fibroblast cell line, Pro5.
0.9727	Epitope - tagged wild - type hPAR ( 2 ) ( wt - hPAR ( 2 ) ) or hPAR ( 2 ) that lacked glycosylation sequons ( following site - directed mutagenesis ) in either the N - terminus Protein[ hPAR ( 2 ) N30A ( Asn ( 30 ) - - > Ala ) ], extracellular loop 2 [ ECL2 ; hPAR ( 2 ) N222Q ( Asn ( 222 ) - - > Gln ) or hPAR ( 2 ) N222A ( Asn ( 222 ) - - > Ala ) ] or both ( hPAR ( 2 ) N30A, N222A or hPAR ( 2 ) N30A, N222Q ) were expressed in the Chinese - hamster ovary ( CHO ) fibroblast cell line, Pro5.
0.9585	Epitope - tagged wild - type hPAR ( 2 ) ( wt - hPAR ( 2 ) ) or hPAR ( 2 ) that lacked glycosylation sequons ( following site - directed mutagenesis ) in either the N - terminus [ hPAR ( 2 ) N30A ( Asn ( 30 ) - - > Ala ) ], extracellular loop 2 [ ECL2 ; hPAR ( 2 ) N222Q ( Asn ( 222 ) - - > Gln ) or ProteinhPAR ( 2 ) N222A ( Asn ( 222 ) - - > Ala ) ] or both ( hPAR ( 2 ) N30A, N222A or hPAR ( 2 ) N30A, N222Q ) were expressed in the Chinese - hamster ovary ( CHO ) fibroblast cell line, Pro5.
0.8938	Epitope - tagged wild - type hPAR ( 2 ) ( wt - hPAR ( 2 ) ) or hPAR ( 2 ) that lacked glycosylation sequons ( following site - directed mutagenesis ) in either the N - terminus [ hPAR ( 2 ) N30A ( Asn ( 30 ) - - > Ala ) ], extracellular loop 2 [ ECL2 ; ProteinhPAR ( 2 ) N222Q ( Asn ( 222 ) - - > Gln ) or hPAR ( 2 ) N222A ( Asn ( 222 ) - - > Ala ) ] or both ( hPAR ( 2 ) N30A, N222A or hPAR ( 2 ) N30A, N222Q ) were expressed in the Chinese - hamster ovary ( CHO ) fibroblast cell line, Pro5.
0.9945	Epitope - tagged wild - type ProteinhPAR ( 2 ) ( wt - hPAR ( 2 ) ) or hPAR ( 2 ) that lacked glycosylation sequons ( following site - directed mutagenesis ) in either the N - terminus [ hPAR ( 2 ) N30A ( Asn ( 30 ) - - > Ala ) ], extracellular loop 2 [ ECL2 ; hPAR ( 2 ) N222Q ( Asn ( 222 ) - - > Gln ) or hPAR ( 2 ) N222A ( Asn ( 222 ) - - > Ala ) ] or both ( hPAR ( 2 ) N30A, N222A or hPAR ( 2 ) N30A, N222Q ) were expressed in the Chinese - hamster ovary ( CHO ) fibroblast cell line, Pro5.

(689)

Western blot analysis of wt - hPAR ( 2 ) showed mature wt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following N - glycosidase F deglycosylation.

Precision: 80.00

Recall: 80.00

F1: 80.00

Score	Text
0.9982	Western blot analysis of Proteinwt - hPAR ( 2 ) showed mature wt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following N - glycosidase F deglycosylation.
0.9982	Western blot analysis of wt - hPAR ( 2 ) showed mature Proteinwt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following N - glycosidase F deglycosylation.
0.9305	Western blot analysis of wt - hPAR ( 2 ) showed mature wt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following ProteinN - glycosidase F deglycosylation.
0.4976	Western blot analysis of wt - hPAR ( 2 ) showed mature wt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following N - glycosidase F Catalysisdeglycosylation .
0.9227	Western blot analysis of wt - hPAR ( 2 ) showed mature wt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following N Protein- glycosidase F deglycosylation.
	Western blot analysis of wt - hPAR ( 2 ) showed mature wt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following N - glycosidase F Deglycosylationdeglycosylation .

(690)

FACS analysis and immunocytochemistry of the wt - hPAR ( 2 ) and PAR ( 2 ) mutant cell lines revealed that removal of both glycosylation sequons decreases ( 50 % of wt - hPAR ( 2 ) ) cell surface expression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	FACS analysis and immunocytochemistry of the Proteinwt - hPAR ( 2 ) and PAR ( 2 ) mutant cell lines revealed that removal of both glycosylation sequons decreases ( 50 % of wt - hPAR ( 2 ) ) cell surface expression.
0.9998	FACS analysis and immunocytochemistry of the wt - hPAR ( 2 ) and PAR ( 2 ) mutant cell lines revealed that removal of both glycosylation sequons decreases ( 50 % of Proteinwt - hPAR ( 2 ) ) cell surface expression.
0.9998	FACS analysis and immunocytochemistry of the wt - hPAR ( 2 ) and ProteinPAR ( 2 ) mutant cell lines revealed that removal of both glycosylation sequons decreases ( 50 % of wt - hPAR ( 2 ) ) cell surface expression.

(691)

Western blot analysis indicated that both N - linked sites are glycosylated.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9885	Western blot analysis indicated that both N - linked sites are Glycosylationglycosylated .
0.7699	Western blot analysis indicated that both EntityN - linked sites are glycosylated.
0.6745	Western blot analysis indicated that both EntityN - linked sites are glycosylated.

(692)

In functional studies, hPAR ( 2 ) N30A displayed a selective and significant increase in sensitivity towards tryptase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	In functional studies, ProteinhPAR ( 2 ) N30A displayed a selective and significant increase in sensitivity towards tryptase.

(693)

Interestingly, hPAR ( 2 ) N222A displayed a loss in sensitivity towards all PAR ( 2 ) agonists tested.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	Interestingly, hPAR ( 2 ) N222A displayed a loss in sensitivity towards all ProteinPAR ( 2 ) agonists tested.
0.9946	Interestingly, ProteinhPAR ( 2 ) N222A displayed a loss in sensitivity towards all PAR ( 2 ) agonists tested.

(694)

However, further analysis revealed receptor sensitivity to alanine mutations in this domain, as the more conservative substitution hPAR ( 2 ) N222Q displayed no change in response to PAR ( 2 ) agonists. hPAR ( 2 ) N30A, N222Q displayed increased sensitivity towards tryptase, but a loss in sensitivity towards trypsin and the synthetic peptide SLIGRL - NH ( 2 ), although this loss in sensitivity towards trypsin and SLIGRL - NH ( 2 ) was secondary to changes in cell - surface expression.

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Text
0.9997	However, further analysis revealed receptor sensitivity to alanine mutations in this domain, as the more conservative substitution hPAR ( 2 ) N222Q displayed no change in response to ProteinPAR ( 2 ) agonists. hPAR ( 2 ) N30A, N222Q displayed increased sensitivity towards tryptase, but a loss in sensitivity towards trypsin and the synthetic peptide SLIGRL - NH ( 2 ), although this loss in sensitivity towards trypsin and SLIGRL - NH ( 2 ) was secondary to changes in cell - surface expression.
0.9980	However, further analysis revealed receptor sensitivity to alanine mutations in this domain, as the more conservative substitution hPAR ( 2 ) N222Q displayed no change in response to PAR ( 2 ) agonists. ProteinhPAR ( 2 ) N30A , N222Q displayed increased sensitivity towards tryptase, but a loss in sensitivity towards trypsin and the synthetic peptide SLIGRL - NH ( 2 ), although this loss in sensitivity towards trypsin and SLIGRL - NH ( 2 ) was secondary to changes in cell - surface expression.
0.9931	However, further analysis revealed receptor sensitivity to alanine mutations in this domain, as the more conservative substitution ProteinhPAR ( 2 ) N222Q displayed no change in response to PAR ( 2 ) agonists. hPAR ( 2 ) N30A, N222Q displayed increased sensitivity towards tryptase, but a loss in sensitivity towards trypsin and the synthetic peptide SLIGRL - NH ( 2 ), although this loss in sensitivity towards trypsin and SLIGRL - NH ( 2 ) was secondary to changes in cell - surface expression.
0.9966	However, further analysis revealed receptor sensitivity to alanine mutations in this domain, as the more conservative substitution hPAR ( 2 ) N222Q displayed no change in response to PAR ( 2 ) agonists. hPAR ( 2 ) N30A, N222Q displayed increased sensitivity towards tryptase, but a loss in sensitivity towards trypsin and the synthetic peptide ProteinSLIGRL - NH ( 2 ) , although this loss in sensitivity towards trypsin and SLIGRL - NH ( 2 ) was secondary to changes in cell - surface expression.
0.9243	However, further analysis revealed receptor sensitivity to alanine mutations in this domain, as the more conservative substitution hPAR ( 2 ) N222Q displayed no change in response to PAR ( 2 ) agonists. hPAR ( 2 ) N30A, N222Q displayed increased sensitivity towards tryptase, but a loss in sensitivity towards trypsin and the synthetic peptide SLIGRL - NH ( 2 ), although this loss in sensitivity towards trypsin and ProteinSLIGRL - NH ( 2 ) was secondary to changes in cell - surface expression.

(695)

Finally, expression of sialic - acid - deficient wt - hPAR ( 2 ) in the CHO Lec2 glycosylation - deficient mutant cell line, showed a 40 kDa loss in molecular mass, in addition to a marked and selective increase in sensitivity towards tryptase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	Finally, expression of sialic - acid - deficient Proteinwt - hPAR ( 2 ) in the CHO Lec2 glycosylation - deficient mutant cell line, showed a 40 kDa loss in molecular mass, in addition to a marked and selective increase in sensitivity towards tryptase.

(696)

We conclude that hPAR ( 2 ) N - linked glycosylation and sialylation regulates receptor expression and / or signalling.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9991	We conclude that ProteinhPAR ( 2 ) N - linked glycosylation and sialylation regulates receptor expression and / or signalling.
0.8338	We conclude that hPAR ( 2 ) GlycosylationN - linked glycosylation and sialylation regulates receptor expression and / or signalling.
0.8733	We conclude that hPAR ( 2 ) N - linked Glycosylationglycosylation and sialylation regulates receptor expression and / or signalling.
0.6257	We conclude that hPAR ( 2 ) GlycosylationN - linked glycosylation and sialylation regulates receptor expression and / or signalling.

(697)

N - acetyltransferase 2 genotype - related sulfapyridine acetylation and its adverse events.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9201	ProteinN - acetyltransferase 2 genotype - related sulfapyridine acetylation and its adverse events.

(698)

Sulfapyridine ( SP ), one of the metabolites of sulfasalazine ( SASP ), is further metabolized into N - acetylsulfapyridine ( AcSP ) by polymorphic N - acetyltransferase 2 ( NAT2 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9802	Sulfapyridine ( SP ), one of the metabolites of sulfasalazine ( SASP ), is further metabolized into N - acetylsulfapyridine ( AcSP ) by polymorphic N - acetyltransferase 2 Protein( NAT2 ) .
0.8200	Sulfapyridine ( SP ), one of the metabolites of sulfasalazine ( SASP ), is further metabolized into N - acetylsulfapyridine ( AcSP ) by polymorphic ProteinN - acetyltransferase 2 ( NAT2 ).

(699)

NAT2 activity has been diagnosed by phenotyping, that is, evaluating plasma concentrations or urinary excretions of tentatively administered test drugs for dose individualization and avoidance of serious adverse events.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	ProteinNAT2 activity has been diagnosed by phenotyping, that is, evaluating plasma concentrations or urinary excretions of tentatively administered test drugs for dose individualization and avoidance of serious adverse events.

(700)

Herein, we investigated the relationship between NAT2 genotypes and the pharmacokinetics of SP in healthy Japanese subjects, as well as the adverse events of SASP in patients with inflammatory bowel disease ( IBD ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	Herein, we investigated the relationship between ProteinNAT2 genotypes and the pharmacokinetics of SP in healthy Japanese subjects, as well as the adverse events of SASP in patients with inflammatory bowel disease ( IBD ).

(701)

Eight healthy subjects and 13 IBD patients were classified into three groups by NAT2 genotyping ; the homozygote for the wild - type allele ( Rapid Types ), the compound heterozygote for the wild - type and mutant alleles ( Intermediate Types ), and the homozygote for mutant alleles ( Slow Types ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Eight healthy subjects and 13 IBD patients were classified into three groups by ProteinNAT2 genotyping ; the homozygote for the wild - type allele ( Rapid Types ), the compound heterozygote for the wild - type and mutant alleles ( Intermediate Types ), and the homozygote for mutant alleles ( Slow Types ).

(702)

A single oral dose of 40 mg / kg SASP was administered to each healthy subject, and plasma and urine samples were taken until 51 and 72 h after administration, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(703)

Both the SP and AcSP concentrations in each sample were determined by the HPLC method.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(704)

The NAT2 genotypes were well - correlated with the plasma concentrations or urinary excretions of SP and AcSP in 8 healthy subjects, except for one Slow Type.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	The ProteinNAT2 genotypes were well - correlated with the plasma concentrations or urinary excretions of SP and AcSP in 8 healthy subjects, except for one Slow Type.

(705)

In patients with IBD, skin rash was seen in 3 of 6 Rapid Types and 1 of 6 Intermediate Types, consistent with the concept that hypersensitive reactions are independent of serum SP concentrations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(706)

In contrast, SASP dosing - related acute pancreatitis was found in the Slow Type patient.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(707)

In this case, the NAT2 activity was diagnosed by genotyping in advance, and the medical staff could pay scrupulous attention, resulting in no serious subjective symptoms such as abdominal pain, anorexia or fever.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	In this case, the ProteinNAT2 activity was diagnosed by genotyping in advance, and the medical staff could pay scrupulous attention, resulting in no serious subjective symptoms such as abdominal pain, anorexia or fever.

(708)

Further investigations on the relationship between the NAT2 genotype and adverse events are required, although genotyping appeared to be a promising method to avoid such serious adverse events.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	Further investigations on the relationship between the ProteinNAT2 genotype and adverse events are required, although genotyping appeared to be a promising method to avoid such serious adverse events.

(709)

Histone H3 lysine 4 methylation is mediated by Set1 and promotes maintenance of active chromatin states in fission yeast.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	Histone H3 lysine 4 methylation is mediated by ProteinSet1 and promotes maintenance of active chromatin states in fission yeast.
0.9720	Histone H3 lysine 4 Methylationmethylation is mediated by Set1 and promotes maintenance of active chromatin states in fission yeast.
0.8466	ProteinHistone H3 lysine 4 methylation is mediated by Set1 and promotes maintenance of active chromatin states in fission yeast.
0.7979	Histone H3 Entitylysine 4 methylation is mediated by Set1 and promotes maintenance of active chromatin states in fission yeast.
0.6807	Histone H3 lysine 4 methylation is Catalysismediated by Set1 and promotes maintenance of active chromatin states in fission yeast.

(710)

Methylation of histone H3 at lysine 4 ( H3 Lys - 4 ) or lysine 9 ( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9432	Methylation of Proteinhistone H3 at lysine 4 ( H3 Lys - 4 ) or lysine 9 ( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.
0.9095	MethylationMethylation of histone H3 at lysine 4 ( H3 Lys - 4 ) or lysine 9 ( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.
0.8615	Methylation of histone H3 at lysine 4 ( H3 Lys - 4 ) or lysine 9 ( H3 EntityLys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.
0.8457	Methylation of histone H3 at Entitylysine 4 ( H3 Lys - 4 ) or lysine 9 ( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.
0.8383	Methylation of histone H3 at lysine 4 ( H3 Lys - 4 ) or Entitylysine 9 ( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.
0.7948	Methylation of histone H3 at lysine 4 ( H3 EntityLys - 4 ) or lysine 9 ( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.
0.6926	Methylation of histone H3 at lysine 4 ( H3 Lys - 4 ) or lysine 9 Protein( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.
0.6748	Methylation of histone H3 at lysine 4 Protein( H3 Lys - 4 ) or lysine 9 ( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.
0.5307	Methylation of histone H3 at lysine 4 ( H3 Lys - 4 ) or lysine 9 Entity( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.
0.4543	Methylation of histone H3 at lysine 4 Entity( H3 Lys - 4 ) or lysine 9 ( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.

(711)

However, in budding yeast, H3 Lys - 4 methylation is also necessary for silent chromatin assembly at telomeres and ribosomal DNA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9806	However, in budding yeast, H3 Lys - 4 Methylationmethylation is also necessary for silent chromatin assembly at telomeres and ribosomal DNA.
0.9711	However, in budding yeast, H3 EntityLys - 4 methylation is also necessary for silent chromatin assembly at telomeres and ribosomal DNA.
0.9638	However, in budding yeast, ProteinH3 Lys - 4 methylation is also necessary for silent chromatin assembly at telomeres and ribosomal DNA.

(712)

Here we demonstrate that deletion of set1, which encodes a protein containing an RNA recognition motif at its amino terminus and a SET domain at the carboxy terminus, abolishes H3 Lys - 4 methylation in fission yeast.

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9995	Here we demonstrate that deletion of Proteinset1 , which encodes a protein containing an RNA recognition motif at its amino terminus and a SET domain at the carboxy terminus, abolishes H3 Lys - 4 methylation in fission yeast.
0.9882	Here we demonstrate that deletion of set1, which encodes a protein containing an RNA recognition motif at its amino terminus and a SET domain at the carboxy terminus, abolishes ProteinH3 Lys - 4 methylation in fission yeast.
0.9817	Here we demonstrate that deletion of set1, which encodes a protein containing an RNA recognition motif at its amino terminus and a SET domain at the carboxy terminus, abolishes H3 EntityLys - 4 methylation in fission yeast.
0.9727	Here we demonstrate that deletion of set1, which encodes a protein containing an RNA recognition motif at its amino terminus and a SET domain at the carboxy terminus, abolishes H3 Lys - 4 Methylationmethylation in fission yeast.
0.5062	Here we demonstrate that deletion of set1, which encodes a protein containing an RNA recognition motif at its amino terminus and a SET domain at the carboxy terminus, abolishes EntityH3 Lys - 4 methylation in fission yeast.

(713)

Unlike in budding yeast, Set1 - mediated H3 Lys - 4 methylation is not required for heterochromatin assembly at the silent mating - type region and centromeres in fission yeast.

Precision: 80.00

Recall: 80.00

F1: 80.00

Score	Text
0.9135	Unlike in budding yeast, Set1 - mediated H3 Lys - 4 Methylationmethylation is not required for heterochromatin assembly at the silent mating - type region and centromeres in fission yeast.
0.6949	Unlike in budding yeast, CatalysisSet1 - mediated H3 Lys - 4 methylation is not required for heterochromatin assembly at the silent mating - type region and centromeres in fission yeast.
0.6140	Unlike in budding yeast, Set1 - mediated ProteinH3 Lys - 4 methylation is not required for heterochromatin assembly at the silent mating - type region and centromeres in fission yeast.
0.5954	Unlike in budding yeast, Set1 - mediated H3 EntityLys - 4 methylation is not required for heterochromatin assembly at the silent mating - type region and centromeres in fission yeast.
0.4851	Unlike in budding yeast, ProteinSet1 - mediated H3 Lys - 4 methylation is not required for heterochromatin assembly at the silent mating - type region and centromeres in fission yeast.
	Unlike in budding yeast, ProteinSet1 - mediated H3 Lys - 4 methylation is not required for heterochromatin assembly at the silent mating - type region and centromeres in fission yeast.

(714)

Our analysis suggests that H3 Lys - 4 methylation is a stable histone modification present throughout the cell cycle, including mitosis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9939	Our analysis suggests that H3 Lys - 4 methylation is a stable Proteinhistone modification present throughout the cell cycle, including mitosis.
0.9929	Our analysis suggests that H3 Lys - 4 Methylationmethylation is a stable histone modification present throughout the cell cycle, including mitosis.
0.9775	Our analysis suggests that ProteinH3 Lys - 4 methylation is a stable histone modification present throughout the cell cycle, including mitosis.
0.9744	Our analysis suggests that H3 EntityLys - 4 methylation is a stable histone modification present throughout the cell cycle, including mitosis.

(715)

The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and acetylation of the H3 tail.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9968	The loss of H3 Lys - 4 methylation in Proteinset1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and acetylation of the H3 tail.
0.9868	The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 Acetylationacetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and acetylation of the H3 tail.
0.9852	The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and acetylation of the ProteinH3 tail.
0.9817	The loss of H3 Lys - 4 Methylationmethylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and acetylation of the H3 tail.
0.9795	The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 Methylationmethylation and acetylation of the H3 tail.
0.9785	The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between ProteinH3 Lys - 4 methylation and acetylation of the H3 tail.
0.9783	The loss of ProteinH3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and acetylation of the H3 tail.
0.9774	The loss of H3 EntityLys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and acetylation of the H3 tail.
0.9662	The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 EntityLys - 4 methylation and acetylation of the H3 tail.
0.9568	The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in Proteinhistone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and acetylation of the H3 tail.
0.9528	The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and acetylation of the H3 Entitytail .
0.9435	The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and Acetylationacetylation of the H3 tail.

(716)

We suggest that methylation of H3 Lys - 4 primarily acts in the maintenance of transcriptionally poised euchromatic domains, and that this modification is dispensable for heterochromatin formation in fission yeast, which instead utilizes H3 Lys - 9 methylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9887	We suggest that methylation of H3 Lys - 4 primarily acts in the maintenance of transcriptionally poised euchromatic domains, and that this modification is dispensable for heterochromatin formation in fission yeast, which instead utilizes H3 Lys - 9 Methylationmethylation .
0.9856	We suggest that methylation of H3 Lys - 4 primarily acts in the maintenance of transcriptionally poised euchromatic domains, and that this modification is dispensable for heterochromatin formation in fission yeast, which instead utilizes ProteinH3 Lys - 9 methylation.
0.9843	We suggest that Methylationmethylation of H3 Lys - 4 primarily acts in the maintenance of transcriptionally poised euchromatic domains, and that this modification is dispensable for heterochromatin formation in fission yeast, which instead utilizes H3 Lys - 9 methylation.
0.9743	We suggest that methylation of ProteinH3 Lys - 4 primarily acts in the maintenance of transcriptionally poised euchromatic domains, and that this modification is dispensable for heterochromatin formation in fission yeast, which instead utilizes H3 Lys - 9 methylation.
0.9669	We suggest that methylation of H3 EntityLys - 4 primarily acts in the maintenance of transcriptionally poised euchromatic domains, and that this modification is dispensable for heterochromatin formation in fission yeast, which instead utilizes H3 Lys - 9 methylation.
0.9653	We suggest that methylation of H3 Lys - 4 primarily acts in the maintenance of transcriptionally poised euchromatic domains, and that this modification is dispensable for heterochromatin formation in fission yeast, which instead utilizes H3 EntityLys - 9 methylation.

(717)

Hypoxia - inducible factor asparaginyl hydroxylase ( FIH - 1 ) catalyses hydroxylation at the beta - carbon of asparagine - 803.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9643	Hypoxia - inducible factor asparaginyl hydroxylase Protein( FIH - 1 ) catalyses hydroxylation at the beta - carbon of asparagine - 803.
0.6652	ProteinHypoxia - inducible factor asparaginyl hydroxylase ( FIH - 1 ) catalyses hydroxylation at the beta - carbon of asparagine - 803.

(718)

Asparagine - 803 in the C - terminal transactivation domain of human hypoxia - inducible factor ( HIF ) - 1 alpha - subunit is hydroxylated by factor inhibiting HIF - 1 ( FIH - 1 ) under normoxic conditions causing abrogation of the HIF - 1alpha / p300 interaction.

Precision: 100.00

Recall: 85.71

F1: 92.31

Score	Text
0.9913	Asparagine - 803 in the C - terminal transactivation domain of human hypoxia - inducible factor ( HIF ) - 1 alpha - subunit is hydroxylated by factor inhibiting HIF - 1 ( FIH - 1 ) under normoxic conditions causing abrogation of the ProteinHIF - 1alpha / p300 interaction.
0.9737	EntityAsparagine - 803 in the C - terminal transactivation domain of human hypoxia - inducible factor ( HIF ) - 1 alpha - subunit is hydroxylated by factor inhibiting HIF - 1 ( FIH - 1 ) under normoxic conditions causing abrogation of the HIF - 1alpha / p300 interaction.
0.9419	Asparagine - 803 in the C - terminal transactivation domain of human hypoxia - inducible factor ( HIF ) - 1 alpha - subunit is hydroxylated by factor inhibiting HIF - 1 Protein( FIH - 1 ) under normoxic conditions causing abrogation of the HIF - 1alpha / p300 interaction.
0.8053	Asparagine - 803 in the C - terminal transactivation domain of human hypoxia - inducible factor ( HIF ) - 1 alpha - subunit is hydroxylated by Proteinfactor inhibiting HIF - 1 ( FIH - 1 ) under normoxic conditions causing abrogation of the HIF - 1alpha / p300 interaction.
0.6968	Asparagine - 803 in the C - terminal transactivation domain of human hypoxia - inducible factor ( HIF ) - 1 alpha - subunit is Hydroxylationhydroxylated by factor inhibiting HIF - 1 ( FIH - 1 ) under normoxic conditions causing abrogation of the HIF - 1alpha / p300 interaction.
0.5802	Asparagine - 803 in the C - terminal transactivation domain of human Proteinhypoxia - inducible factor ( HIF ) - 1 alpha - subunit is hydroxylated by factor inhibiting HIF - 1 ( FIH - 1 ) under normoxic conditions causing abrogation of the HIF - 1alpha / p300 interaction.
	Asparagine - 803 in the C - terminal transactivation domain of human hypoxia - inducible factor ( HIF ) - 1 alpha - subunit is Catalysishydroxylated by factor inhibiting HIF - 1 ( FIH - 1 ) under normoxic conditions causing abrogation of the HIF - 1alpha / p300 interaction.

(719)

NMR and other analyses of a hydroxylated HIF fragment produced in vitro demonstrate that hydroxylation occurs at the beta - carbon of Asn - 803 and imply production of the threo - isomer, in contrast with other known aspartic acid / asparagine hydroxylases that produce the erythro - isomer.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9586	NMR and other analyses of a hydroxylated HIF fragment produced in vitro demonstrate that hydroxylation occurs at the beta - carbon of EntityAsn - 803 and imply production of the threo - isomer, in contrast with other known aspartic acid / asparagine hydroxylases that produce the erythro - isomer.
0.9496	NMR and other analyses of a hydroxylated HIF fragment produced in vitro demonstrate that Hydroxylationhydroxylation occurs at the beta - carbon of Asn - 803 and imply production of the threo - isomer, in contrast with other known aspartic acid / asparagine hydroxylases that produce the erythro - isomer.
0.7227	NMR and other analyses of a Hydroxylationhydroxylated HIF fragment produced in vitro demonstrate that hydroxylation occurs at the beta - carbon of Asn - 803 and imply production of the threo - isomer, in contrast with other known aspartic acid / asparagine hydroxylases that produce the erythro - isomer.

(720)

Effect of the alcohol extract of the seeds of Mucuna pruriens on free radicals and oxidative stress in albino rats.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(721)

In vitro and in vivo studies were made with an alcohol extract of the seeds of Mucuna pruriens ( Fabaceae ) to investigate its antioxidant property.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(722)

In vitro studies were carried out in rat liver homogenate to investigate the chemical interaction of various phytochemicals with different species of free radicals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(723)

The effect was also checked on iron - induced lipid peroxidation, oxidation of GSH content, and its interaction with hydroxyl and superoxide radicals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(724)

There was no change on the rate of aerial oxidation of GSH content but it significantly inhibited FeSO ( 4 ) induced lipid peroxidation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(725)

It also inhibited the specific chemical reactions induced by superoxides and hydroxyl radicals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(726)

The removal of these species was through direct chemical interaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(727)

An in vivo study on albino rats for 30 days showed no toxic effect up to a dose of 600 mg / kg body weight, on oral administration.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(728)

There was no change in the level of TBA - reactive substances, reduced glutathione content and SOD activity in the liver.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(729)

The activity of serum GOT, GPT and alkaline phosphatase was also unchanged.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	The activity of serum GOT, ProteinGPT and alkaline phosphatase was also unchanged.
0.9984	The activity of serum ProteinGOT , GPT and alkaline phosphatase was also unchanged.

(730)

Thus it could be concluded that the alcohol extract of the seeds of M. pruriens has an antilipid peroxidation property, which is mediated through the removal of superoxides and hydroxyl radicals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(731)

Induction of apoptosis of human B - CLL and ALL cells by a novel retinoid and its nonretinoidal analog.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(732)

We have recently described a novel retinoid 6 - [ 3 - ( 1 - adamantyl ) - 4 - hydroxyphenyl ] - 2 - naphthalenecarboxylic acid ( CD437 / AHPN ) that induces apoptosis in a number of malignant cell types.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(733)

We now describe our studies examining the effects of CD437 and a nonretinoidal analog ( MM002 ) on the in vitro proliferation of the ALL - REH cell line, the in vitro and in vivo growth of a novel Epstein - Barr virus - negative ( EBV ( - ) ) B - cell chronic lymphocytic leukemia ( B - CLL ) cell line ( WSU - CLL ), and primary cultures of human B - CLL and acute lymphoblastic leukemia ( ALL ) cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(734)

CD437 and MM002 induce apoptosis in both cell lines, as indicated by the activation of caspase - 2 and caspase - 3, cleavage of poly ( adenosine diphosphate - ribose ) ( poly ( ADP - ribose ) ) polymerase, increase in annexin V binding, and subsequent nuclear fragmentation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9981	CD437 and MM002 induce apoptosis in both cell lines, as indicated by the activation of caspase - 2 and Proteincaspase - 3 , cleavage of poly ( adenosine diphosphate - ribose ) ( poly ( ADP - ribose ) ) polymerase, increase in annexin V binding, and subsequent nuclear fragmentation.
0.9964	CD437 and MM002 induce apoptosis in both cell lines, as indicated by the activation of Proteincaspase - 2 and caspase - 3, cleavage of poly ( adenosine diphosphate - ribose ) ( poly ( ADP - ribose ) ) polymerase, increase in annexin V binding, and subsequent nuclear fragmentation.
0.9861	CD437 and MM002 induce apoptosis in both cell lines, as indicated by the activation of caspase - 2 and caspase - 3, cleavage of poly ( adenosine diphosphate - ribose ) ( poly ( ADP - ribose ) ) polymerase, increase in Proteinannexin V binding, and subsequent nuclear fragmentation.

(735)

CD437 - mediated apoptosis was not associated with the modulation of Bcl - 2, Bax, or Mcl - 1 levels, but was associated with the cleavage of the antiapoptotic protein Bcl - X ( L ) to a proapoptotic 18 - kD form.

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9996	CD437 - mediated apoptosis was not associated with the modulation of ProteinBcl - 2 , Bax, or Mcl - 1 levels, but was associated with the cleavage of the antiapoptotic protein Bcl - X ( L ) to a proapoptotic 18 - kD form.
0.9994	CD437 - mediated apoptosis was not associated with the modulation of Bcl - 2, Bax, or ProteinMcl - 1 levels, but was associated with the cleavage of the antiapoptotic protein Bcl - X ( L ) to a proapoptotic 18 - kD form.
0.9993	CD437 - mediated apoptosis was not associated with the modulation of Bcl - 2, ProteinBax , or Mcl - 1 levels, but was associated with the cleavage of the antiapoptotic protein Bcl - X ( L ) to a proapoptotic 18 - kD form.
0.9830	CD437 - mediated apoptosis was not associated with the modulation of Bcl - 2, Bax, or Mcl - 1 levels, but was associated with the cleavage of the antiapoptotic protein ProteinBcl - X ( L ) to a proapoptotic 18 - kD form.
0.6090	ProteinCD437 - mediated apoptosis was not associated with the modulation of Bcl - 2, Bax, or Mcl - 1 levels, but was associated with the cleavage of the antiapoptotic protein Bcl - X ( L ) to a proapoptotic 18 - kD form.

(736)

This cleavage of Bcl - X ( L ) was dependent on caspase - 3 activation since Bcl - X ( L ) cleavage and apoptosis were inhibited by the caspase - 3 inhibitor Z - DVED - fmk.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9979	This cleavage of ProteinBcl - X ( L ) was dependent on caspase - 3 activation since Bcl - X ( L ) cleavage and apoptosis were inhibited by the caspase - 3 inhibitor Z - DVED - fmk.
0.9970	This cleavage of Bcl - X ( L ) was dependent on Proteincaspase - 3 activation since Bcl - X ( L ) cleavage and apoptosis were inhibited by the caspase - 3 inhibitor Z - DVED - fmk.
0.9960	This cleavage of Bcl - X ( L ) was dependent on caspase - 3 activation since ProteinBcl - X ( L ) cleavage and apoptosis were inhibited by the caspase - 3 inhibitor Z - DVED - fmk.
0.9914	This cleavage of Bcl - X ( L ) was dependent on caspase - 3 activation since Bcl - X ( L ) cleavage and apoptosis were inhibited by the Proteincaspase - 3 inhibitor Z - DVED - fmk.

(737)

CD437 markedly inhibited the growth of WSU - CLL cells in severe combined immunodeficiency ( SCID ) mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(738)

Tumor growth inhibition, growth delay, and log cell kill were 85. 7 %, 21 days, and 2. 1, respectively, in the treated mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(739)

Moreover, 1 of the 5 treated mice was tumor - free longer than 150 days and thus was considered cured.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(740)

Exposure of primary cultures of both B - CLL and ALL cells obtained from patients to CD437 and MM002 resulted in their apoptosis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(741)

These results suggest that CD437 and MM002 analogs may have a potential role in the treatment of B - CLL and ALL.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(742)

Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia - inducible factor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(743)

The product of the von Hippel - Lindau gene, pVHL, targets the alpha subunits of the heterodimeric transcription factor hypoxia - inducible factor ( HIF ) for polyubiquitination in the presence of oxygen.

Precision: 40.00

Recall: 100.00

F1: 57.14

Score	Text
0.9995	The product of the von Hippel - Lindau gene, ProteinpVHL , targets the alpha subunits of the heterodimeric transcription factor hypoxia - inducible factor ( HIF ) for polyubiquitination in the presence of oxygen.
0.5735	The product of the Proteinvon Hippel - Lindau gene , pVHL, targets the alpha subunits of the heterodimeric transcription factor hypoxia - inducible factor ( HIF ) for polyubiquitination in the presence of oxygen.
0.8365	The product of the Proteinvon Hippel - Lindau gene, pVHL, targets the alpha subunits of the heterodimeric transcription factor hypoxia - inducible factor ( HIF ) for polyubiquitination in the presence of oxygen.
0.6372	The product of the von ProteinHippel - Lindau gene, pVHL, targets the alpha subunits of the heterodimeric transcription factor hypoxia - inducible factor ( HIF ) for polyubiquitination in the presence of oxygen.
0.6196	The product of the von Hippel - Lindau gene, pVHL, targets the alpha subunits of the heterodimeric transcription factor hypoxia - inducible factor ( HIF ) for Ubiquitinationpolyubiquitination in the presence of oxygen.

(744)

The binding of pVHL to HIF is governed by the enzymatic hydroxylation of conserved prolyl residues within peptidic motifs present in the HIFalpha family members.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9995	The binding of ProteinpVHL to HIF is governed by the enzymatic hydroxylation of conserved prolyl residues within peptidic motifs present in the HIFalpha family members.
0.6625	The binding of pVHL to HIF is governed by the enzymatic Hydroxylationhydroxylation of conserved prolyl residues within peptidic motifs present in the HIFalpha family members.

(745)

By using a biochemical purification strategy, we have identified a human homolog of Caenorhabditis elegans Egl9 as a HIF prolyl hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	By using a biochemical purification strategy, we have identified a human homolog of Caenorhabditis elegans ProteinEgl9 as a HIF prolyl hydroxylase.

(746)

In addition, we studied the activity of a structurally diverse collection of low molecular weight inhibitors of procollagen prolyl 4 - hydroxylase as potential inhibitors of the HIF hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(747)

A model compound of this series stabilized HIF in a variety of cells, leading to the increased production of its downstream target, vascular endothelial growth factor.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.8641	A model compound of this series stabilized HIF in a variety of cells, leading to the increased production of its downstream target, Proteinvascular endothelial growth factor .
0.5768	A model compound of this series stabilized HIF in a variety of cells, leading to the increased production of its downstream target, vascular Proteinendothelial growth factor .

(748)

Control of CBP co - activating activity by arginine methylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9573	Control of CBP co - activating activity by arginine Methylationmethylation .
0.9331	Control of ProteinCBP co - activating activity by arginine methylation.
0.9331	Control of CBP co - activating activity by Entityarginine methylation.

(749)

The histone acetyltransferases CREB binding protein ( CBP ) and the related p300 protein function as key transcriptional co - activators in multiple pathways.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9977	The histone acetyltransferases CREB binding protein ( CBP ) and the related Proteinp300 protein function as key transcriptional co - activators in multiple pathways.
0.9973	The Proteinhistone acetyltransferases CREB binding protein ( CBP ) and the related p300 protein function as key transcriptional co - activators in multiple pathways.
0.9768	The histone acetyltransferases CREB binding protein Protein( CBP ) and the related p300 protein function as key transcriptional co - activators in multiple pathways.
0.8809	The histone acetyltransferases ProteinCREB binding protein ( CBP ) and the related p300 protein function as key transcriptional co - activators in multiple pathways.

(750)

In the case of transcriptional activation by nuclear receptors, ligand promotes the recruitment of co - activators of the p160 family, such as GRIP - 1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9983	In the case of transcriptional activation by nuclear receptors, ligand promotes the recruitment of co - activators of the p160 family, such as ProteinGRIP - 1 .

(751)

Subsequently, the p160 co - activators recruit other co - activators via two activation domains, AD1 and AD2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(752)

AD1 binds CBP or p300, whereas AD2 has been shown to activate transcription through the recruitment of the arginine methyltransferase CARM1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	AD1 binds CBP or p300, whereas AD2 has been shown to activate transcription through the recruitment of the arginine methyltransferase ProteinCARM1 .
0.9996	AD1 binds CBP or Proteinp300 , whereas AD2 has been shown to activate transcription through the recruitment of the arginine methyltransferase CARM1.
0.9992	AD1 binds ProteinCBP or p300, whereas AD2 has been shown to activate transcription through the recruitment of the arginine methyltransferase CARM1.

(753)

Recently, the KIX domain of CBP has been shown to be methylated by CARM1 in vitro.

Precision: 80.00

Recall: 80.00

F1: 80.00

Score	Text
0.9981	Recently, the KIX domain of CBP has been shown to be methylated by ProteinCARM1 in vitro.
0.9929	Recently, the KIX domain of ProteinCBP has been shown to be methylated by CARM1 in vitro.
0.7963	Recently, the EntityKIX domain of CBP has been shown to be methylated by CARM1 in vitro.
0.5521	Recently, the KIX domain of CBP has been shown to be Catalysismethylated by CARM1 in vitro.
0.5713	Recently, the EntityKIX domain of CBP has been shown to be methylated by CARM1 in vitro.
	Recently, the KIX domain of CBP has been shown to be Methylationmethylated by CARM1 in vitro.

(754)

Here, we report that another domain of CBP is specifically methylated by CARM1 on conserved arginine residues in vitro and in vivo.

Precision: 100.00

Recall: 75.00

F1: 85.71

Score	Text
0.9996	Here, we report that another domain of ProteinCBP is specifically methylated by CARM1 on conserved arginine residues in vitro and in vivo.
0.9994	Here, we report that another domain of CBP is specifically methylated by ProteinCARM1 on conserved arginine residues in vitro and in vivo.
0.5629	Here, we report that another domain of CBP is specifically Catalysismethylated by CARM1 on conserved arginine residues in vitro and in vivo.
	Here, we report that another domain of CBP is specifically Methylationmethylated by CARM1 on conserved arginine residues in vitro and in vivo.

(755)

We also provide functional evidence that arginine residues methylated by CARM1 play a critical role in GRIP - 1 - dependent transcriptional activation and in hormone - induced gene activation.

Precision: 80.00

Recall: 80.00

F1: 80.00

Score	Text
0.9991	We also provide functional evidence that arginine residues methylated by ProteinCARM1 play a critical role in GRIP - 1 - dependent transcriptional activation and in hormone - induced gene activation.
0.9973	We also provide functional evidence that arginine residues methylated by CARM1 play a critical role in ProteinGRIP - 1 - dependent transcriptional activation and in hormone - induced gene activation.
0.8416	We also provide functional evidence that Entityarginine residues methylated by CARM1 play a critical role in GRIP - 1 - dependent transcriptional activation and in hormone - induced gene activation.
0.4810	We also provide functional evidence that arginine residues Methylationmethylated by CARM1 play a critical role in GRIP - 1 - dependent transcriptional activation and in hormone - induced gene activation.
0.7698	We also provide functional evidence that Entityarginine residues methylated by CARM1 play a critical role in GRIP - 1 - dependent transcriptional activation and in hormone - induced gene activation.
	We also provide functional evidence that arginine residues Catalysismethylated by CARM1 play a critical role in GRIP - 1 - dependent transcriptional activation and in hormone - induced gene activation.

(756)

Altogether, our data provide strong evidence that arginine methylation represents an important mechanism for modulating co - activator transcriptional activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(757)

The hepatitis C virus RNA - dependent RNA polymerase membrane insertion sequence is a transmembrane segment.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9200	The hepatitis C virus ProteinRNA - dependent RNA polymerase membrane insertion sequence is a transmembrane segment.
0.6697	The hepatitis C virus RNA - dependent ProteinRNA polymerase membrane insertion sequence is a transmembrane segment.

(758)

The hepatitis C virus ( HCV ) RNA - dependent RNA polymerase ( RdRp ) belongs to a class of membrane proteins termed tail - anchored proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9795	The hepatitis C virus ( HCV ) RNA - dependent RNA polymerase Protein( RdRp ) belongs to a class of membrane proteins termed tail - anchored proteins.
0.8625	The hepatitis C virus ( HCV ) ProteinRNA - dependent RNA polymerase ( RdRp ) belongs to a class of membrane proteins termed tail - anchored proteins.

(759)

Here, we show that the HCV RdRp C - terminal membrane insertion sequence traverses the phospholipid bilayer as a transmembrane segment.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	Here, we show that the HCV ProteinRdRp C - terminal membrane insertion sequence traverses the phospholipid bilayer as a transmembrane segment.

(760)

Moreover, the HCV RdRp was found to be retained in the endoplasmic reticulum ( ER ) or an ER - derived modified compartment both following transient transfection and in the context of a subgenomic replicon.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Moreover, the HCV ProteinRdRp was found to be retained in the endoplasmic reticulum ( ER ) or an ER - derived modified compartment both following transient transfection and in the context of a subgenomic replicon.

(761)

An absolutely conserved GVG motif was not essential for membrane insertion but possibly provides a docking site for transmembrane protein - protein interactions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(762)

These findings have important implications for the functional architecture of the HCV replication complex.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(763)

Molecular and biochemical characterisation of a novel sulphatase gene : Arylsulfatase G ( ARSG ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9678	Molecular and biochemical characterisation of a novel sulphatase gene : Arylsulfatase G Protein( ARSG ) .
0.8697	Molecular and biochemical characterisation of a novel sulphatase gene : ProteinArylsulfatase G ( ARSG ).

(764)

Molecular analysis has provided important insights into the biochemistry and genetics of the sulphatase family of enzymes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(765)

Through bioinformatic searches of the EST database, we have identified a novel gene consisting of 11 exons and encoding a 525 aa protein that shares a high degree of sequence similarity with all sulphatases and in particular with arylsulphatases, hence the tentative name Arylsulfatase G ( ARSG ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9786	Through bioinformatic searches of the EST database, we have identified a novel gene consisting of 11 exons and encoding a 525 aa protein that shares a high degree of sequence similarity with all sulphatases and in particular with arylsulphatases, hence the tentative name Arylsulfatase G Protein( ARSG ) .
0.9090	Through bioinformatic searches of the EST database, we have identified a novel gene consisting of 11 exons and encoding a 525 aa protein that shares a high degree of sequence similarity with all sulphatases and in particular with arylsulphatases, hence the tentative name ProteinArylsulfatase G ( ARSG ).

(766)

The highest homology is shared with Arylsulfatase A, a lysosomal sulphatase which is mutated in metachromatic leukodistrophy, particularly in the amino - terminal region.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9628	The highest homology is shared with ProteinArylsulfatase A , a lysosomal sulphatase which is mutated in metachromatic leukodistrophy, particularly in the amino - terminal region.

(767)

The 10 amino acids that form the catalytic site are strongly conserved.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(768)

The murine homologue of Arylsulfatase G gene product shows 87 % identity with the human protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9778	The murine homologue of ProteinArylsulfatase G gene product shows 87 % identity with the human protein.

(769)

To test the function of this novel gene we transfected the full - length cDNA in Cos7 cells, and detected an Arylsulfatase G precursor protein of 62 kDa.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9556	To test the function of this novel gene we transfected the full - length cDNA in Cos7 cells, and detected an ProteinArylsulfatase G precursor protein of 62 kDa.

(770)

After glycosylation the precursor is maturated in a 70 kDa form, which localises to the endoplasmic reticulum.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9557	After Glycosylationglycosylation the precursor is maturated in a 70 kDa form, which localises to the endoplasmic reticulum.

(771)

Northern blot analysis of Arylsulfatase G revealed a ubiquitous expression pattern.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9614	Northern blot analysis of ProteinArylsulfatase G revealed a ubiquitous expression pattern.

(772)

We tested the sulphatase activity towards two different artificial substrates 4 - methylumbelliferyl ( 4 - MU ) sulphate and p - nitrocatechol sulphate, but no arylsulphatase activity was detectable.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(773)

Further studies are needed to characterise the function of Arylsulfatase G, possibly revealing a novel metabolic pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9161	Further studies are needed to characterise the function of ProteinArylsulfatase G , possibly revealing a novel metabolic pathway.

(774)

Gene - specific targeting of H3K9 methylation is sufficient for initiating repression in vivo.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9915	Gene - specific targeting of H3K9 Methylationmethylation is sufficient for initiating repression in vivo.
0.4964	Gene - specific targeting of EntityH3K9 methylation is sufficient for initiating repression in vivo.
	Gene - specific targeting of ProteinH3K9 methylation is sufficient for initiating repression in vivo.

(775)

Covalent modifications of chromatin have emerged as key determinants of the genome's transcriptional competence.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(776)

Histone H3 lysine 9 ( H3K9 ) methylation is an epigenetic signal that is recognized by HP1 and correlates with gene silencing in a variety of organisms.

Precision: 100.00

Recall: 80.00

F1: 88.89

Score	Text
0.9796	Histone H3 lysine 9 ( H3K9 ) Methylationmethylation is an epigenetic signal that is recognized by HP1 and correlates with gene silencing in a variety of organisms.
0.8773	Histone H3 Entitylysine 9 ( H3K9 ) methylation is an epigenetic signal that is recognized by HP1 and correlates with gene silencing in a variety of organisms.
0.8742	ProteinHistone H3 lysine 9 ( H3K9 ) methylation is an epigenetic signal that is recognized by HP1 and correlates with gene silencing in a variety of organisms.
0.6420	Histone H3 lysine 9 Protein( H3K9 ) methylation is an epigenetic signal that is recognized by HP1 and correlates with gene silencing in a variety of organisms.
	Histone H3 lysine 9 Entity( H3K9 ) methylation is an epigenetic signal that is recognized by HP1 and correlates with gene silencing in a variety of organisms.

(777)

Discovery of the enzymes that catalyze H3K9 methylation has identified a second gene - specific function for this modification in transcriptional repression.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9932	Discovery of the enzymes that catalyze H3K9 Methylationmethylation has identified a second gene - specific function for this modification in transcriptional repression.
0.5202	Discovery of the enzymes that catalyze EntityH3K9 methylation has identified a second gene - specific function for this modification in transcriptional repression.
	Discovery of the enzymes that catalyze ProteinH3K9 methylation has identified a second gene - specific function for this modification in transcriptional repression.

(778)

Whether H3K9 methylation is causative in the initiation and establishment of gene repression or is a byproduct of the process leading to the repressed state remains unknown.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9751	Whether H3K9 Methylationmethylation is causative in the initiation and establishment of gene repression or is a byproduct of the process leading to the repressed state remains unknown.
0.6680	Whether EntityH3K9 methylation is causative in the initiation and establishment of gene repression or is a byproduct of the process leading to the repressed state remains unknown.
	Whether ProteinH3K9 methylation is causative in the initiation and establishment of gene repression or is a byproduct of the process leading to the repressed state remains unknown.

(779)

To investigate the role of HMTs and specifically H3K9 methylation in gene repression, we have employed engineered zinc - finger transcription factors ( ZFPs ) to target HMT activity to a specific endogenous gene.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9816	To investigate the role of HMTs and specifically H3K9 Methylationmethylation in gene repression, we have employed engineered zinc - finger transcription factors ( ZFPs ) to target HMT activity to a specific endogenous gene.
0.6073	To investigate the role of HMTs and specifically EntityH3K9 methylation in gene repression, we have employed engineered zinc - finger transcription factors ( ZFPs ) to target HMT activity to a specific endogenous gene.
	To investigate the role of HMTs and specifically ProteinH3K9 methylation in gene repression, we have employed engineered zinc - finger transcription factors ( ZFPs ) to target HMT activity to a specific endogenous gene.

(780)

By utilizing ZFPs that recognize the promoter of the endogenous VEGF - A gene, and thus employing this chromosomal locus as an in vivo reporter, we show that ZFPs linked to a minimal catalytic HMT domain affect local methylation of histone H3K9 and the consequent repression of target gene expression.

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9995	By utilizing ZFPs that recognize the promoter of the endogenous ProteinVEGF - A gene, and thus employing this chromosomal locus as an in vivo reporter, we show that ZFPs linked to a minimal catalytic HMT domain affect local methylation of histone H3K9 and the consequent repression of target gene expression.
0.9609	By utilizing ZFPs that recognize the promoter of the endogenous VEGF - A gene, and thus employing this chromosomal locus as an in vivo reporter, we show that ZFPs linked to a minimal catalytic HMT domain affect local Methylationmethylation of histone H3K9 and the consequent repression of target gene expression.
0.9582	By utilizing ZFPs that recognize the promoter of the endogenous VEGF - A gene, and thus employing this chromosomal locus as an in vivo reporter, we show that ZFPs linked to a minimal catalytic HMT domain affect local methylation of histone EntityH3K9 and the consequent repression of target gene expression.
0.5971	By utilizing ZFPs that recognize the promoter of the endogenous VEGF - A gene, and thus employing this chromosomal locus as an in vivo reporter, we show that ZFPs linked to a minimal catalytic HMT domain affect local methylation of Proteinhistone H3K9 and the consequent repression of target gene expression.
0.5517	By utilizing ZFPs that recognize the Entitypromoter of the endogenous VEGF - A gene, and thus employing this chromosomal locus as an in vivo reporter, we show that ZFPs linked to a minimal catalytic HMT domain affect local methylation of histone H3K9 and the consequent repression of target gene expression.

(781)

Furthermore, amino acid substitutions within the HMT that ablate its catalytic activity effectively eliminate the ability of the ZFP fusions to repress transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(782)

Thus, H3K9 methylation is a primary signal that is sufficient for initiating a gene repression pathway in vivo.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9805	Thus, H3K9 Methylationmethylation is a primary signal that is sufficient for initiating a gene repression pathway in vivo.
0.6792	Thus, EntityH3K9 methylation is a primary signal that is sufficient for initiating a gene repression pathway in vivo.
	Thus, ProteinH3K9 methylation is a primary signal that is sufficient for initiating a gene repression pathway in vivo.

(783)

Studies on the inhibition of human cytochromes P450 by selenocysteine Se - conjugates.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(784)

1. To investigate whether cytochrome P450 ( P450 ) inhibition can contribute to the chemopreventive activity of selenocysteine Se - conjugates ( SeCys conjugates ), 21 SeCys conjugates were screened for their inhibitory potency towards seven of the most important human P450s. 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(785)

The majority of the SeCys conjugates produced near complete inhibition of CYP1A1 at a concentration of 250 microm.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	The majority of the SeCys conjugates produced near complete inhibition of ProteinCYP1A1 at a concentration of 250 microm.

(786)

The most potent inhibitor, Se - benzyl - L - selenocysteine, displayed an IC50 of 12. 8 + / - 1. 2 microm.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(787)

CYP2C9, - 2C19 and - 2D6 were moderately ( 50 - 60 % ) inhibited by the SeCys conjugates.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	CYP2C9, Protein- 2C19 and - 2D6 were moderately ( 50 - 60 % ) inhibited by the SeCys conjugates.
0.9982	CYP2C9, - 2C19 and Protein- 2D6 were moderately ( 50 - 60 % ) inhibited by the SeCys conjugates.
0.9980	ProteinCYP2C9 , - 2C19 and - 2D6 were moderately ( 50 - 60 % ) inhibited by the SeCys conjugates.

(788)

CYP1A2, - 2E1 and - 3A4 were least inhibited. 3.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	CYP1A2, Protein- 2E1 and - 3A4 were least inhibited. 3.
0.9985	ProteinCYP1A2 , - 2E1 and - 3A4 were least inhibited. 3.
0.9975	CYP1A2, - 2E1 and Protein- 3A4 were least inhibited. 3.

(789)

Studies on the susceptibility of CYP1A1 to SeCys conjugates implicated a thiol - reactive intermediate, as evidenced by reduced inhibition levels in the presence of glutathione and N - acetyl cysteine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Studies on the susceptibility of ProteinCYP1A1 to SeCys conjugates implicated a thiol - reactive intermediate, as evidenced by reduced inhibition levels in the presence of glutathione and N - acetyl cysteine.

(790)

Uncoupling of the P450 - catalytic cycle was of no importance as ROS scavengers did not influence inhibition levels. 4.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(791)

P450 inhibition by two physiologically relevant metabolite classes of SeCys conjugates was also studied.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(792)

N - acetylation of SeCys conjugates consistently increased the inhibitory potency towards CYP1A2, - 2C19, - 2E1 and - 3A4.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9974	N - acetylation of SeCys conjugates consistently increased the inhibitory potency towards CYP1A2, - 2C19, - 2E1 and Protein- 3A4 .
0.9970	N - acetylation of SeCys conjugates consistently increased the inhibitory potency towards CYP1A2, - 2C19, Protein- 2E1 and - 3A4.
0.9967	N - acetylation of SeCys conjugates consistently increased the inhibitory potency towards ProteinCYP1A2 , - 2C19, - 2E1 and - 3A4.
0.9956	N - acetylation of SeCys conjugates consistently increased the inhibitory potency towards CYP1A2, Protein- 2C19 , - 2E1 and - 3A4.

(793)

Beta - lyase catalysed bioactivation of alkyl - substituted SeCys conjugates or Se - benzyl - L - selenocysteine produced little or no additional inhibition of P450 activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(794)

For Se - phenyl - L - selenocysteine, however, significant increases in P450 inhibition were obtained by beta - lyase pre - incubation. 5.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(795)

It is concluded that the potent and relatively selective CYP1A1 inhibition exerted by SeCys conjugates may contribute to their chemopreventive activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	It is concluded that the potent and relatively selective ProteinCYP1A1 inhibition exerted by SeCys conjugates may contribute to their chemopreventive activity.

(796)

Identification of a lactoferrin - derived peptide possessing binding activity to hepatitis C virus E2 envelope protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	Identification of a lactoferrin - derived peptide possessing binding activity to hepatitis C virus ProteinE2 envelope protein.
0.9945	Identification of a Proteinlactoferrin - derived peptide possessing binding activity to hepatitis C virus E2 envelope protein.

(797)

Bovine and human lactoferrins ( LF ) prevent hepatitis C virus ( HCV ) infection in cultured human hepatocytes ; the preventive mechanism is thought to be the direct interaction between LF and HCV.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	Bovine and human lactoferrins ( LF ) prevent hepatitis C virus ( HCV ) infection in cultured human hepatocytes ; the preventive mechanism is thought to be the direct interaction between ProteinLF and HCV.
0.9830	Bovine and human lactoferrins Protein( LF ) prevent hepatitis C virus ( HCV ) infection in cultured human hepatocytes ; the preventive mechanism is thought to be the direct interaction between LF and HCV.
0.9812	Bovine and human Proteinlactoferrins ( LF ) prevent hepatitis C virus ( HCV ) infection in cultured human hepatocytes ; the preventive mechanism is thought to be the direct interaction between LF and HCV.

(798)

To clarify this hypothesis, we have characterized the binding activity of LF to HCV E2 envelope protein and have endeavored to determine which region ( s ) of LF are important for this binding activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9979	To clarify this hypothesis, we have characterized the binding activity of LF to HCV ProteinE2 envelope protein and have endeavored to determine which region ( s ) of LF are important for this binding activity.
0.9955	To clarify this hypothesis, we have characterized the binding activity of ProteinLF to HCV E2 envelope protein and have endeavored to determine which region ( s ) of LF are important for this binding activity.
0.9952	To clarify this hypothesis, we have characterized the binding activity of LF to HCV E2 envelope protein and have endeavored to determine which region ( s ) of ProteinLF are important for this binding activity.

(799)

Several regions of human LF have been expressed and purified as thioredoxin - fused proteins in Escherichia coli.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	Several regions of human ProteinLF have been expressed and purified as thioredoxin - fused proteins in Escherichia coli.

(800)

Far - Western blot analysis using these LF fragments and the E2 protein, expressed in Chinese hamster ovary cells, revealed that the 93 carboxyl amino acids of LF specifically bound to the E2 protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Far - Western blot analysis using these LF fragments and the E2 protein, expressed in Chinese hamster ovary cells, revealed that the 93 carboxyl amino acids of ProteinLF specifically bound to the E2 protein.
0.9995	Far - Western blot analysis using these LF fragments and the ProteinE2 protein, expressed in Chinese hamster ovary cells, revealed that the 93 carboxyl amino acids of LF specifically bound to the E2 protein.
0.9993	Far - Western blot analysis using these LF fragments and the E2 protein, expressed in Chinese hamster ovary cells, revealed that the 93 carboxyl amino acids of LF specifically bound to the ProteinE2 protein.
0.9976	Far - Western blot analysis using these ProteinLF fragments and the E2 protein, expressed in Chinese hamster ovary cells, revealed that the 93 carboxyl amino acids of LF specifically bound to the E2 protein.

(801)

The 93 carboxyl amino acids of LFs derived from bovine and horse cells also possessed similar binding activity to the E2 protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	The 93 carboxyl amino acids of ProteinLFs derived from bovine and horse cells also possessed similar binding activity to the E2 protein.
0.9995	The 93 carboxyl amino acids of LFs derived from bovine and horse cells also possessed similar binding activity to the ProteinE2 protein.

(802)

In addition, the amino acid sequences of these carboxyl regions appeared to show partial homology to CD81, a candidate receptor for HCV, and the binding activity of these carboxyl regions was also comparable with that of CD81.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	In addition, the amino acid sequences of these carboxyl regions appeared to show partial homology to ProteinCD81 , a candidate receptor for HCV, and the binding activity of these carboxyl regions was also comparable with that of CD81.
0.9993	In addition, the amino acid sequences of these carboxyl regions appeared to show partial homology to CD81, a candidate receptor for HCV, and the binding activity of these carboxyl regions was also comparable with that of ProteinCD81 .

(803)

Further deletion analysis identified 33 amino acid residues as the minimum binding site in the carboxyl region of LF, and the binding specificity of these 33 amino acids was also confirmed by using 33 maltose - binding protein - fused amino acids.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9982	Further deletion analysis identified 33 amino acid residues as the minimum binding site in the carboxyl region of ProteinLF , and the binding specificity of these 33 amino acids was also confirmed by using 33 maltose - binding protein - fused amino acids.

(804)

Furthermore, we demonstrated that the 33 maltose - binding protein - fused amino acids prevented HCV infection in cultured human hepatocytes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9200	Furthermore, we demonstrated that the 33 Proteinmaltose - binding protein - fused amino acids prevented HCV infection in cultured human hepatocytes.

(805)

In addition, the site - directed mutagenesis to an Ala residue in both terminal residues of the 33 amino acids revealed that Cys at amino acid 628 was determined to be critical for binding to the E2 protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	In addition, the site - directed mutagenesis to an Ala residue in both terminal residues of the 33 amino acids revealed that Cys at amino acid 628 was determined to be critical for binding to the ProteinE2 protein.

(806)

These results led us to consider the development of an effective anti - HCV peptide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(807)

This is the first identification of a natural protein - derived peptide that specifically binds to HCV E2 protein and prevents HCV infection.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	This is the first identification of a natural protein - derived peptide that specifically binds to HCV ProteinE2 protein and prevents HCV infection.

(808)

Glucocorticoid suppression of nuclear factor - kappa B : a role for histone modifications.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9970	Glucocorticoid suppression of nuclear factor - kappa B : a role for Proteinhistone modifications.

(809)

Corticosteroids are by far the most effective treatment for chronic inflammatory diseases such as asthma.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(810)

Inflammation in asthma is characterized by the increased expression of multiple inflammatory genes, including those that encode cytokines, chemokines, adhesion molecules, and inflammatory enzymes and receptors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(811)

Increased expression of inflammatory genes is regulated by pro - inflammatory transcription factors, such as nuclear factor kappa B ( NF - kappa B ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(812)

These bind to, and activate, co - activator molecules that then acetylate core histones resulting in elevated gene transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9980	These bind to, and activate, co - activator molecules that then acetylate core Proteinhistones resulting in elevated gene transcription.
0.9838	These bind to, and activate, co - activator molecules that then Acetylationacetylate core histones resulting in elevated gene transcription.

(813)

Corticosteroids reverse histone acetylation at the site of inflammatory gene transcription, either by direct binding of the activated glucocorticoid receptor to NF - kappa B - associated co - activators or by recruitment of histone deacetylases to the activated transcription complex.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	Corticosteroids reverse histone acetylation at the site of inflammatory gene transcription, either by direct binding of the activated glucocorticoid receptor to NF - kappa B - associated co - activators or by recruitment of Proteinhistone deacetylases to the activated transcription complex.
0.9971	Corticosteroids reverse Proteinhistone acetylation at the site of inflammatory gene transcription, either by direct binding of the activated glucocorticoid receptor to NF - kappa B - associated co - activators or by recruitment of histone deacetylases to the activated transcription complex.
0.9951	Corticosteroids reverse histone Acetylationacetylation at the site of inflammatory gene transcription, either by direct binding of the activated glucocorticoid receptor to NF - kappa B - associated co - activators or by recruitment of histone deacetylases to the activated transcription complex.
0.9606	Corticosteroids reverse histone acetylation at the site of inflammatory gene transcription, either by direct binding of the activated Proteinglucocorticoid receptor to NF - kappa B - associated co - activators or by recruitment of histone deacetylases to the activated transcription complex.

(814)

Understanding how corticosteroids work in asthma may help in designing novel corticosteroids with fewer systemic effects, as well as novel anti - inflammatory approaches.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(815)

Probing the conformational and dynamical effects of O - glycosylation within the immunodominant region of a MUC1 peptide tumor antigen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9929	Probing the conformational and dynamical effects of O - glycosylation within the immunodominant region of a ProteinMUC1 peptide tumor antigen.
0.9927	Probing the conformational and dynamical effects of GlycosylationO - glycosylation within the immunodominant region of a MUC1 peptide tumor antigen.
0.7962	Probing the conformational and dynamical effects of O - glycosylation within the Entityimmunodominant region of a MUC1 peptide tumor antigen.

(816)

MUC1 mucin is a large transmembrane glycoprotein, the extracellular domain of which is formed by a repeating 20 amino acid sequence, GVTSAPDTRPAPGSTAPPAH.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9826	ProteinMUC1 mucin is a large transmembrane glycoprotein, the extracellular domain of which is formed by a repeating 20 amino acid sequence, GVTSAPDTRPAPGSTAPPAH.
0.5706	MUC1 mucin is a large transmembrane glycoprotein, the extracellular domain of which is formed by a repeating 20 amino acid sequence, ProteinGVTSAPDTRPAPGSTAPPAH .

(817)

In normal breast epithelial cells, the extracellular domain is densely covered with highly branched complex carbohydrate structures.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(818)

However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.

Precision: 26.92

Recall: 87.50

F1: 41.18

Score	Text
0.9895	However, in neoplastic breast tissue, the extracellular domain is Glycosylationunder - glycosylated , resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.9833	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core EntityTn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.9695	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), EntitySTn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.9624	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and EntityTF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.9619	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn Entity( GalNAc ) , STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.8804	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF Entity( Gal beta1 - 3 GalNAc ) carbohydrates.
0.8337	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn Entity( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.9785	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 EntityGalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.9575	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 EntityGalNAc ) carbohydrates.
0.9523	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core EntityTn ( GalNAc ) , STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.9232	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl Entityalpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.9076	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal Entitybeta1 - 3 GalNAc ) carbohydrates.
0.8761	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and EntityTF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.8266	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), EntitySTn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.7873	However, in neoplastic breast tissue, the extracellular Entitydomain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.7721	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl Entityalpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.7588	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and EntityTF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.7582	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal Entitybeta1 - 3 GalNAc ) carbohydrates.
0.7263	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF Entity( Gal beta1 - 3 GalNAc ) carbohydrates.
0.7236	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and EntityTF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.6652	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), EntitySTn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.6415	However, in neoplastic breast tissue, the Glycosylationextracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.6345	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF Entity( Gal beta1 - 3 GalNAc ) carbohydrates.
0.6316	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn Entity( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.6209	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), EntitySTn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.
0.5170	However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 EntityGalNAc ) carbohydrates .
	However, in neoplastic breast tissue, the Entityextracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.

(819)

Here, we report the results of 1H NMR structural studies, natural abundance 13C NMR relaxation measurements and distance - restrained MD simulations designed to probe the structural and dynamical effects of Tn - glycosylation within the PDTRP core peptide epitope.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.6019	Here, we report the results of 1H NMR structural studies, natural abundance 13C NMR relaxation measurements and distance - restrained MD simulations designed to probe the structural and dynamical effects of GlycosylationTn - glycosylation within the PDTRP core peptide epitope.

(820)

Two synthetic peptides were studied : a nine - residue MUC1 peptide of the sequence, Thr1 - Ser2 - Ala3 - Pro4 - Asp5 - Thr6 - Arg7 - Pro8 - Ala9, and a Tn - glycosylated version of this peptide, Thr1 - Ser2 - Ala3 - Pro4 - Asp5 - Thr6 ( alphaGalNAc ) - Arg7 - Pro8 - Ala9.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Text
0.9968	Two synthetic peptides were studied : a nine - residue ProteinMUC1 peptide of the sequence, Thr1 - Ser2 - Ala3 - Pro4 - Asp5 - Thr6 - Arg7 - Pro8 - Ala9, and a Tn - glycosylated version of this peptide, Thr1 - Ser2 - Ala3 - Pro4 - Asp5 - Thr6 ( alphaGalNAc ) - Arg7 - Pro8 - Ala9.
0.9156	Two synthetic peptides were studied : a nine - residue MUC1 peptide of the sequence, Thr1 - Ser2 - Ala3 - Pro4 - Asp5 - Thr6 - Arg7 - Pro8 - Ala9, and a GlycosylationTn - glycosylated version of this peptide, Thr1 - Ser2 - Ala3 - Pro4 - Asp5 - Thr6 ( alphaGalNAc ) - Arg7 - Pro8 - Ala9.
0.6593	Two synthetic peptides were studied : a nine - residue MUC1 peptide of the sequence, EntityThr1 - Ser2 - Ala3 - Pro4 - Asp5 - Thr6 - Arg7 - Pro8 - Ala9 , and a Tn - glycosylated version of this peptide, Thr1 - Ser2 - Ala3 - Pro4 - Asp5 - Thr6 ( alphaGalNAc ) - Arg7 - Pro8 - Ala9.

(821)

The results of these studies show that a type I beta - turn conformation is adopted by residues PDTR within the PDTRP region of the unglycosylated MUC1 sequence.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9973	The results of these studies show that a type I beta - turn conformation is adopted by residues PDTR within the PDTRP region of the unglycosylated ProteinMUC1 sequence.
0.9303	The results of these studies show that a type I beta - turn conformation is adopted by residues PDTR within the PDTRP region of the Glycosylationunglycosylated MUC1 sequence.

(822)

The existence of a similar beta - turn within the PDTRP core peptide epitope of the under - glycosylated cancer - associated MUC1 mucin protein might explain the immunodominance of this region in vivo, as the presence of defined secondary structure within peptide epitope regions has been correlated with increased immunogenicity in other systems.

Precision: 66.67

Recall: 66.67

F1: 66.67

Score	Text
0.9935	The existence of a similar beta - turn within the PDTRP core peptide epitope of the Glycosylationunder - glycosylated cancer - associated MUC1 mucin protein might explain the immunodominance of this region in vivo, as the presence of defined secondary structure within peptide epitope regions has been correlated with increased immunogenicity in other systems.
0.9255	The existence of a similar beta - turn within the PDTRP core peptide epitope of the under - glycosylated cancer - associated ProteinMUC1 mucin protein might explain the immunodominance of this region in vivo, as the presence of defined secondary structure within peptide epitope regions has been correlated with increased immunogenicity in other systems.
0.7533	The existence of a similar beta - turn within the PDTRP core peptide Entityepitope of the under - glycosylated cancer - associated MUC1 mucin protein might explain the immunodominance of this region in vivo, as the presence of defined secondary structure within peptide epitope regions has been correlated with increased immunogenicity in other systems.
	The existence of a similar beta - turn within the EntityPDTRP core peptide epitope of the under - glycosylated cancer - associated MUC1 mucin protein might explain the immunodominance of this region in vivo, as the presence of defined secondary structure within peptide epitope regions has been correlated with increased immunogenicity in other systems.

(823)

Our results have also shown that Tn glycosylation at the central threonine within the PDTRP core epitope region shifts the conformational equilibrium away from the type I beta - turn conformation and toward a more rigid and extended state.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.8089	Our results have also shown that Tn Glycosylationglycosylation at the central threonine within the PDTRP core epitope region shifts the conformational equilibrium away from the type I beta - turn conformation and toward a more rigid and extended state.

(824)

The significance of these results are discussed in relation to the possible roles that peptide epitope secondary structure and glycosylation state may play in MUC1 tumor immunogenicity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	The significance of these results are discussed in relation to the possible roles that peptide epitope secondary structure and glycosylation state may play in ProteinMUC1 tumor immunogenicity.
0.9714	The significance of these results are discussed in relation to the possible roles that peptide epitope secondary structure and Glycosylationglycosylation state may play in MUC1 tumor immunogenicity.

(825)

Lysine - 79 of histone H3 is hypomethylated at silenced loci in yeast and mammalian cells : a potential mechanism for position - effect variegation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9743	EntityLysine - 79 of histone H3 is hypomethylated at silenced loci in yeast and mammalian cells : a potential mechanism for position - effect variegation.
0.9735	Lysine - 79 of histone H3 is Methylationhypomethylated at silenced loci in yeast and mammalian cells : a potential mechanism for position - effect variegation.
0.9558	Lysine - 79 of Proteinhistone H3 is hypomethylated at silenced loci in yeast and mammalian cells : a potential mechanism for position - effect variegation.

(826)

Methylation of lysine - 79 ( K79 ) within the globular domain of histone H3 by Dot1 methylase is important for transcriptional silencing and for association of the Sir silencing proteins in yeast.

Precision: 100.00

Recall: 83.33

F1: 90.91

Score	Text
0.9465	Methylation of lysine - 79 ( K79 ) within the globular domain of Proteinhistone H3 by Dot1 methylase is important for transcriptional silencing and for association of the Sir silencing proteins in yeast.
0.9322	Methylation of lysine - 79 ( K79 ) within the globular domain of histone H3 by ProteinDot1 methylase is important for transcriptional silencing and for association of the Sir silencing proteins in yeast.
0.9133	Methylation of lysine - 79 Entity( K79 ) within the globular domain of histone H3 by Dot1 methylase is important for transcriptional silencing and for association of the Sir silencing proteins in yeast.
0.8890	Methylation of Entitylysine - 79 ( K79 ) within the globular domain of histone H3 by Dot1 methylase is important for transcriptional silencing and for association of the Sir silencing proteins in yeast.
0.7087	MethylationMethylation of lysine - 79 ( K79 ) within the globular domain of histone H3 by Dot1 methylase is important for transcriptional silencing and for association of the Sir silencing proteins in yeast.
	CatalysisMethylation of lysine - 79 ( K79 ) within the globular domain of histone H3 by Dot1 methylase is important for transcriptional silencing and for association of the Sir silencing proteins in yeast.

(827)

Here, we show that the level of H3 - K79 methylation is low at all Sir - dependent silenced loci but not at other transcriptionally repressed regions.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9696	Here, we show that the level of H3 - K79 Methylationmethylation is low at all Sir - dependent silenced loci but not at other transcriptionally repressed regions.
0.4977	Here, we show that the level of EntityH3 - K79 methylation is low at all Sir - dependent silenced loci but not at other transcriptionally repressed regions.
	Here, we show that the level of ProteinH3 - K79 methylation is low at all Sir - dependent silenced loci but not at other transcriptionally repressed regions.

(828)

Hypomethylation of H3 - K79 at the telomeric and silent mating - type loci, but not the ribosomal DNA, requires the Sir proteins.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.8828	MethylationHypomethylation of H3 - K79 at the telomeric and silent mating - type loci, but not the ribosomal DNA, requires the Sir proteins.
0.5668	Hypomethylation of ProteinH3 - K79 at the telomeric and silent mating - type loci, but not the ribosomal DNA, requires the Sir proteins.
	Hypomethylation of EntityH3 - K79 at the telomeric and silent mating - type loci, but not the ribosomal DNA, requires the Sir proteins.

(829)

Overexpression of Sir3 concomitantly extends the domain of Sir protein association and H3 - K79 hypomethylation at telomeres.

Precision: 100.00

Recall: 75.00

F1: 85.71

Score	Text
0.9980	Overexpression of ProteinSir3 concomitantly extends the domain of Sir protein association and H3 - K79 hypomethylation at telomeres.
0.9036	Overexpression of Sir3 concomitantly extends the domain of Sir protein association and H3 - K79 Methylationhypomethylation at telomeres.
0.6012	Overexpression of Sir3 concomitantly extends the domain of Sir protein association and EntityH3 - K79 hypomethylation at telomeres.
	Overexpression of Sir3 concomitantly extends the domain of Sir protein association and ProteinH3 - K79 hypomethylation at telomeres.

(830)

In mammalian cells, H3 - K79 methylation is found at loci that are active for V ( D ) J recombination, but not at recombinationally inactive loci that are heterochromatic.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9888	In mammalian cells, H3 - K79 Methylationmethylation is found at loci that are active for V ( D ) J recombination, but not at recombinationally inactive loci that are heterochromatic.
0.5263	In mammalian cells, EntityH3 - K79 methylation is found at loci that are active for V ( D ) J recombination, but not at recombinationally inactive loci that are heterochromatic.
	In mammalian cells, ProteinH3 - K79 methylation is found at loci that are active for V ( D ) J recombination, but not at recombinationally inactive loci that are heterochromatic.

(831)

These results suggest that H3 - K79 methylation is an evolutionarily conserved marker of active chromatin regions, and that silencing proteins block the ability of Dot1 to methylate histone H3.

Precision: 100.00

Recall: 71.43

F1: 83.33

Score	Text
0.9973	These results suggest that H3 - K79 methylation is an evolutionarily conserved marker of active chromatin regions, and that silencing proteins block the ability of ProteinDot1 to methylate histone H3.
0.9796	These results suggest that H3 - K79 Methylationmethylation is an evolutionarily conserved marker of active chromatin regions, and that silencing proteins block the ability of Dot1 to methylate histone H3.
0.9361	These results suggest that H3 - K79 methylation is an evolutionarily conserved marker of active chromatin regions, and that silencing proteins block the ability of Dot1 to methylate Proteinhistone H3 .
0.9027	These results suggest that H3 - K79 methylation is an evolutionarily conserved marker of active chromatin regions, and that silencing proteins block the ability of Dot1 to Methylationmethylate histone H3.
0.5730	These results suggest that ProteinH3 - K79 methylation is an evolutionarily conserved marker of active chromatin regions, and that silencing proteins block the ability of Dot1 to methylate histone H3.
	These results suggest that EntityH3 - K79 methylation is an evolutionarily conserved marker of active chromatin regions, and that silencing proteins block the ability of Dot1 to methylate histone H3.
	These results suggest that H3 - K79 methylation is an evolutionarily conserved marker of active chromatin regions, and that silencing proteins block the ability of Dot1 to Catalysismethylate histone H3.

(832)

Further, they suggest that Sir proteins preferentially bind chromatin with hypomethylated H3 - K79 and then block H3 - K79 methylation.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9930	Further, they suggest that Sir proteins preferentially bind chromatin with hypomethylated H3 - K79 and then block H3 - K79 Methylationmethylation .
0.9891	Further, they suggest that Sir proteins preferentially bind chromatin with Methylationhypomethylated H3 - K79 and then block H3 - K79 methylation.
0.5344	Further, they suggest that Sir proteins preferentially bind chromatin with hypomethylated EntityH3 - K79 and then block H3 - K79 methylation.
0.5262	Further, they suggest that Sir proteins preferentially bind chromatin with hypomethylated H3 - K79 and then block EntityH3 - K79 methylation.
	Further, they suggest that Sir proteins preferentially bind chromatin with hypomethylated ProteinH3 - K79 and then block H3 - K79 methylation.
	Further, they suggest that Sir proteins preferentially bind chromatin with hypomethylated H3 - K79 and then block ProteinH3 - K79 methylation.

(833)

This positive feedback loop, and the reverse loop in which H3 - K79 methylation weakens Sir protein association and leads to further methylation, suggests a model for position - effect variegation.

Precision: 100.00

Recall: 66.67

F1: 80.00

Score	Text
0.9815	This positive feedback loop, and the reverse loop in which H3 - K79 Methylationmethylation weakens Sir protein association and leads to further methylation, suggests a model for position - effect variegation.
0.4951	This positive feedback loop, and the reverse loop in which ProteinH3 - K79 methylation weakens Sir protein association and leads to further methylation, suggests a model for position - effect variegation.
	This positive feedback loop, and the reverse loop in which EntityH3 - K79 methylation weakens Sir protein association and leads to further methylation, suggests a model for position - effect variegation.

(834)

Cyclosporin A prevents the hypoxic adaptation by activating hypoxia - inducible factor - 1alpha Pro - 564 hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9717	Cyclosporin A prevents the hypoxic adaptation by activating hypoxia - inducible factor - 1alpha EntityPro - 564 hydroxylation.
0.9477	Cyclosporin A prevents the hypoxic adaptation by activating hypoxia - inducible factor - 1alpha Pro - 564 Hydroxylationhydroxylation .
0.6583	Cyclosporin A prevents the hypoxic adaptation by activating Proteinhypoxia - inducible factor - 1alpha Pro - 564 hydroxylation.

(835)

The mechanism by which hypoxia induces gene transcription involves the inhibition of hypoxia - inducible factor ( HIF ) - 1alpha prolyl hydroxylase activity, which prevents von Hippel - Lindau ( vHL ) - dependent targeting of HIF - 1alpha to the ubiquitin - proteasome pathway.

Precision: 80.00

Recall: 80.00

F1: 80.00

Score	Text
0.9987	The mechanism by which hypoxia induces gene transcription involves the inhibition of hypoxia - inducible factor ( HIF ) - 1alpha prolyl hydroxylase activity, which prevents von Hippel - Lindau ( vHL ) - dependent targeting of ProteinHIF - 1alpha to the ubiquitin - proteasome pathway.
0.9985	The mechanism by which hypoxia induces gene transcription involves the inhibition of hypoxia - inducible factor ( HIF ) - 1alpha prolyl hydroxylase activity, which prevents von Hippel - Lindau ( vHL ) - dependent targeting of HIF - 1alpha to the Proteinubiquitin - proteasome pathway.
0.9351	The mechanism by which hypoxia induces gene transcription involves the inhibition of hypoxia - inducible factor ( HIF ) - 1alpha prolyl hydroxylase activity, which prevents von Hippel - Lindau Protein( vHL ) - dependent targeting of HIF - 1alpha to the ubiquitin - proteasome pathway.
0.6907	The mechanism by which hypoxia induces gene transcription involves the inhibition of hypoxia - inducible factor ( HIF ) - 1alpha prolyl hydroxylase activity, which prevents Proteinvon Hippel - Lindau ( vHL ) - dependent targeting of HIF - 1alpha to the ubiquitin - proteasome pathway.
0.5809	The mechanism by which hypoxia induces gene transcription involves the inhibition of Proteinhypoxia - inducible factor ( HIF ) - 1alpha prolyl hydroxylase activity, which prevents von Hippel - Lindau ( vHL ) - dependent targeting of HIF - 1alpha to the ubiquitin - proteasome pathway.
	The mechanism by which hypoxia induces gene transcription involves the inhibition of Proteinhypoxia - inducible factor ( HIF ) - 1alpha prolyl hydroxylase activity, which prevents von Hippel - Lindau ( vHL ) - dependent targeting of HIF - 1alpha to the ubiquitin - proteasome pathway.

(836)

HIF - 1alpha is stabilized, translocates to the nucleus, interacts with hypoxia - responsive elements, and promotes the activation of target genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	ProteinHIF - 1alpha is stabilized, translocates to the nucleus, interacts with hypoxia - responsive elements, and promotes the activation of target genes.

(837)

This report shows that cyclosporin A ( CsA ) interferes with the hypoxic signaling cascade in C6 glioma cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(838)

CsA inhibits hypoxia - dependent gene transcription in a reporter gene assay and prevents the hypoxic accumulation of HIF - 1alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9979	CsA inhibits hypoxia - dependent gene transcription in a reporter gene assay and prevents the hypoxic accumulation of ProteinHIF - 1alpha .

(839)

Addition of the 530 - 603 C - terminal oxygen - dependent degradation ( ODD ) domain of HIF - 1alpha to the green fluorescent protein ( GFP ) destabilized the protein in an oxygen - dependent manner.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9992	Addition of the 530 - 603 C - terminal oxygen - dependent degradation ( ODD ) domain of ProteinHIF - 1alpha to the green fluorescent protein ( GFP ) destabilized the protein in an oxygen - dependent manner.
0.9878	Addition of the 530 - 603 C - terminal oxygen - dependent degradation ( ODD ) domain of HIF - 1alpha to the green fluorescent protein Protein( GFP ) destabilized the protein in an oxygen - dependent manner.
0.9178	Addition of the 530 - 603 C - terminal oxygen - dependent degradation ( ODD ) domain of HIF - 1alpha to the Proteingreen fluorescent protein ( GFP ) destabilized the protein in an oxygen - dependent manner.
0.6449	Addition of the 530 - 603 C - terminal oxygen - dependent degradation ( ODD ) domain of HIF - 1alpha to the green Proteinfluorescent protein ( GFP ) destabilized the protein in an oxygen - dependent manner.

(840)

CsA prevented the hypoxic stabilization of an ODD. GFP fusion protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9812	CsA prevented the hypoxic stabilization of an ODD Protein. GFP fusion protein.

(841)

An assay for 2 - oxoglutarate - dependent dioxygenases was developed using a light mitochondrial kidney fraction as a source of enzyme.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(842)

It uses the capacity of specific peptides to stimulate the degradation of [ ( 14 ) C ] 2 - oxoglutarate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(843)

CsA stimulated the enzymatic activity in the presence of a peptide that mimicked the 557 - 576 sequence of HIF - 1alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9990	CsA stimulated the enzymatic activity in the presence of a peptide that mimicked the 557 - 576 sequence of ProteinHIF - 1alpha .

(844)

The enzyme promoted [ ( 35 ) S ] vHL binding to glutathione S - transferase ( GST ). ODD fusion protein.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9857	The enzyme promoted Protein[ ( 35 ) S ] vHL binding to glutathione S - transferase ( GST ). ODD fusion protein.
0.6056	The enzyme promoted [ ( 35 ) S ] vHL binding to Proteinglutathione S - transferase ( GST ). ODD fusion protein.

(845)

This association increased in the presence of CsA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(846)

CsA effects were not observed when the proline residue corresponding to Pro - 564 in the HIF - 1alpha sequence was replaced by a hydroxyproline or an alanine residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	CsA effects were not observed when the proline residue corresponding to Pro - 564 in the ProteinHIF - 1alpha sequence was replaced by a hydroxyproline or an alanine residue.

(847)

Finally, CsA increased vHL - ODD interaction during hypoxia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	Finally, CsA increased ProteinvHL - ODD interaction during hypoxia.

(848)

We conclude that CsA destabilizes HIF - 1alpha by promoting hydroxylation of Pro - 564 in the ODD domain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	We conclude that CsA destabilizes ProteinHIF - 1alpha by promoting hydroxylation of Pro - 564 in the ODD domain.
0.9597	We conclude that CsA destabilizes HIF - 1alpha by promoting Hydroxylationhydroxylation of Pro - 564 in the ODD domain.
0.9593	We conclude that CsA destabilizes HIF - 1alpha by promoting hydroxylation of EntityPro - 564 in the ODD domain.

(849)

Such a mechanism may prevent hypoxic adaptation during CsA - induced nephrotoxicity and contribute to the adverse effects of this drug.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(850)

A role for N - glycanase in the cytosolic turnover of glycoproteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(851)

Successful maturation determines the intracellular fate of secretory and membrane proteins in the endoplasmic reticulum ( ER ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(852)

Failure of proteins to fold or assemble properly can lead to their retention in the ER and redirects them to the cytosol for degradation by the proteasome.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(853)

Proteasome inhibitors can yield deglycosylated cytoplasmic intermediates that are the result of an N - glycanase activity, believed to act prior to destruction of these substrates by the proteasome.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(854)

A gene encoding a yeast peptide : N - glycanase, PNG1, has been cloned, but this N - glycanase and its mammalian homolog were reported to be incapable of deglycosylating full - length glycoproteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	A gene encoding a yeast peptide : N - glycanase, ProteinPNG1 , has been cloned, but this N - glycanase and its mammalian homolog were reported to be incapable of deglycosylating full - length glycoproteins.

(855)

We show that both the yeast PNG1 enzyme and its mammalian homolog display N - glycanase activity towards intact glycoproteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	We show that both the yeast ProteinPNG1 enzyme and its mammalian homolog display N - glycanase activity towards intact glycoproteins.

(856)

As substrates, cytosolic PNGase activity prefers proteins containing high - mannose over those bearing complex type oligosaccharides.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	As substrates, cytosolic ProteinPNGase activity prefers proteins containing high - mannose over those bearing complex type oligosaccharides.

(857)

Importantly, PNG1 discriminates between non - native and folded glycoproteins, consistent with a role for N - glycanase in cytoplasmic turnover of glycoproteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	Importantly, ProteinPNG1 discriminates between non - native and folded glycoproteins, consistent with a role for N - glycanase in cytoplasmic turnover of glycoproteins.

(858)

Preclinical evaluation of antineoplastic activity of inhibitors of DNA methylation ( 5 - aza - 2'- deoxycytidine ) and histone deacetylation ( trichostatin A, depsipeptide ) in combination against myeloid leukemic cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9984	Preclinical evaluation of antineoplastic activity of inhibitors of DNA methylation ( 5 - aza - 2'- deoxycytidine ) and Proteinhistone deacetylation ( trichostatin A, depsipeptide ) in combination against myeloid leukemic cells.
0.8599	Preclinical evaluation of antineoplastic activity of inhibitors of DNA methylation ( 5 - aza - 2'- deoxycytidine ) and histone Deacetylationdeacetylation ( trichostatin A, depsipeptide ) in combination against myeloid leukemic cells.

(859)

During the development of leukemia, genes that suppress growth and induce differentiation can be silenced by aberrant DNA methylation and by changes in chromatin structure that involve histone deacetylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9967	During the development of leukemia, genes that suppress growth and induce differentiation can be silenced by aberrant DNA methylation and by changes in chromatin structure that involve Proteinhistone deacetylation.
0.9130	During the development of leukemia, genes that suppress growth and induce differentiation can be silenced by aberrant DNA methylation and by changes in chromatin structure that involve histone Deacetylationdeacetylation .

(860)

It has been reported that a positive interaction between DNA methylation and histone deacetylation takes place to inhibit transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9962	It has been reported that a positive interaction between DNA methylation and Proteinhistone deacetylation takes place to inhibit transcription.
0.8901	It has been reported that a positive interaction between DNA methylation and histone Deacetylationdeacetylation takes place to inhibit transcription.

(861)

Based on this observation, our working hypothesis was that a combination of inhibitors of these processes should produce an enhancement of their antineoplastic activity on leukemic cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(862)

The cytosine nucleoside analog, 5 - aza - 2'- deoxycytidine ( 5AZA ), is a potent inhibitor of DNA methylation, which can activate tumor suppressor genes in leukemic cells that have been silenced by aberrant methylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(863)

In clinical trials, 5AZA was demonstrated to be an active antileukemic agent.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(864)

Histone deacetylase inhibitors ( HDI ) can also activate gene expression in leukemic cell lines by producing changes in chromatin configuration, and show antineoplastic activity in preclinical studies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	ProteinHistone deacetylase inhibitors ( HDI ) can also activate gene expression in leukemic cell lines by producing changes in chromatin configuration, and show antineoplastic activity in preclinical studies.

(865)

In this report, we investigated the in vitro antineoplastic activity of 5AZA, alone and in combination with the HDI, trichostatin A ( TSA ) and depsipeptide ( FR901228, depsi ), on the human myeloid leukemic cell lines, HL - 60 and KG1a.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(866)

The results showed that the combination of 5AZA with TSA or depsi produced a greater inhibition of growth and DNA synthesis and a greater loss of clonogenicity than either agent alone.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(867)

These results suggest that 5AZA used in combination with HDI may be an interesting chemotherapeutic regimen to investigate in patients with acute myeloid leukemia that is resistant to conventional chemotherapy.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(868)

Complex - type biantennary N - glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [ beta ] - 1, 4 - galactosyltransferase and [ beta ] - 1, 2 - N - acetylglucosaminyltransferase II.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9931	Complex - type biantennary N - glycans of recombinant human Proteintransferrin from Trichoplusia ni insect cells expressing mammalian [ beta ] - 1, 4 - galactosyltransferase and [ beta ] - 1, 2 - N - acetylglucosaminyltransferase II.
0.5402	Complex - type biantennary N - glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [ beta ] - 1, 4 - galactosyltransferase and Protein[ beta ] - 1, 2 - N - acetylglucosaminyltransferase II .
0.6196	Complex - type biantennary N - glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [ beta ] - 1, 4 - galactosyltransferase and [ beta ] - 1 Protein, 2 - N - acetylglucosaminyltransferase II .

(869)

A novel recombinant baculovirus expression vector was used to produce His - tagged human transferrin in a transformed insect cell line ( Tn5beta4GalT ) that constitutively expresses a mammalian beta - 1, 4 - galactosyltransferase.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9994	A novel recombinant baculovirus expression vector was used to produce His - tagged human Proteintransferrin in a transformed insect cell line ( Tn5beta4GalT ) that constitutively expresses a mammalian beta - 1, 4 - galactosyltransferase.
0.7021	A novel recombinant baculovirus expression vector was used to produce His - tagged human transferrin in a transformed insect cell line Protein( Tn5beta4GalT ) that constitutively expresses a mammalian beta - 1, 4 - galactosyltransferase.

(870)

This virus encoded the His - tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9981	This virus encoded the His - tagged human Proteintransferrin protein in conventional fashion under the control of the very late polyhedrin promoter.
0.9892	This virus encoded the His - tagged human transferrin protein in conventional fashion under the control of the very late Proteinpolyhedrin promoter.

(871)

In addition, to enhance the synthesis of galactosylated biantennary N - glycans, this virus encoded human beta - 1, 2 - N - acetylglucosaminyltransferase II under the control of an immediate - early ( ie1 ) promoter.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9649	In addition, to enhance the synthesis of galactosylated biantennary N - glycans, this virus encoded human beta - 1, 2 - N - acetylglucosaminyltransferase II under the control of an immediate - early Protein( ie1 ) promoter.
0.5298	In addition, to enhance the synthesis of galactosylated biantennary N - glycans, this virus encoded human Proteinbeta - 1, 2 - N - acetylglucosaminyltransferase II under the control of an immediate - early ( ie1 ) promoter.
0.6877	In addition, to enhance the synthesis of galactosylated biantennary N - glycans, this virus encoded human beta - 1, 2 - ProteinN - acetylglucosaminyltransferase II under the control of an immediate - early ( ie1 ) promoter.
0.5578	In addition, to enhance the synthesis of galactosylated biantennary N - glycans, this virus encoded human beta - 1 Protein, 2 - N - acetylglucosaminyltransferase II under the control of an immediate - early ( ie1 ) promoter.

(872)

Detailed analyses by MALDI - TOF MS, exoglycosidase digestion, and two - dimensional HPLC revealed that the N - glycans on the purified recombinant human transferrin produced by this virus - host system included four different fully galactosylated, biantennary, complex - type glycans.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	Detailed analyses by MALDI - TOF MS, exoglycosidase digestion, and two - dimensional HPLC revealed that the N - glycans on the purified recombinant human Proteintransferrin produced by this virus - host system included four different fully galactosylated, biantennary, complex - type glycans.

(873)

Thus, this study describes a novel baculovirus - host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N - glycans.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(874)

Overexpression of the CT GalNAc transferase inhibits muscular dystrophy in a cleavage - resistant dystroglycan mutant mouse.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9169	Overexpression of the ProteinCT GalNAc transferase inhibits muscular dystrophy in a cleavage - resistant dystroglycan mutant mouse.
0.5200	Overexpression of the CT ProteinGalNAc transferase inhibits muscular dystrophy in a cleavage - resistant dystroglycan mutant mouse.

(875)

Transgenic mice that express dystroglycan containing a serine to alanine point mutation at the normal site of cleavage ( DG ( S654A ) ) in their skeletal muscles fail to express endogenously cleaved dystroglycan and have muscular dystrophy [ Neuromusc.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9982	Transgenic mice that express Proteindystroglycan containing a serine to alanine point mutation at the normal site of cleavage ( DG ( S654A ) ) in their skeletal muscles fail to express endogenously cleaved dystroglycan and have muscular dystrophy [ Neuromusc.
0.9977	Transgenic mice that express dystroglycan containing a serine to alanine point mutation at the normal site of cleavage ( DG ( S654A ) ) in their skeletal muscles fail to express endogenously cleaved Proteindystroglycan and have muscular dystrophy [ Neuromusc.
0.9892	Transgenic mice that express dystroglycan containing a serine to alanine point mutation at the normal site of cleavage Protein( DG ( S654A ) ) in their skeletal muscles fail to express endogenously cleaved dystroglycan and have muscular dystrophy [ Neuromusc.

(876)

Disord., in press ].

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(877)

Dystrophic DG ( S654A ) muscles have reduced binding of antibodies, including VIA4 - 1, that recognize carbohydrate antigens on alpha dystroglycan, a finding similar to muscles in some forms of congenital muscular dystrophy.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9897	Dystrophic ProteinDG ( S654A ) muscles have reduced binding of antibodies, including VIA4 - 1, that recognize carbohydrate antigens on alpha dystroglycan, a finding similar to muscles in some forms of congenital muscular dystrophy.
0.8732	Dystrophic DG ( S654A ) muscles have reduced binding of antibodies, including VIA4 - 1, that recognize carbohydrate antigens on Proteinalpha dystroglycan , a finding similar to muscles in some forms of congenital muscular dystrophy.
0.7869	Dystrophic DG ( S654A ) muscles have reduced binding of antibodies, including VIA4 - 1, that recognize carbohydrate antigens on alpha Proteindystroglycan , a finding similar to muscles in some forms of congenital muscular dystrophy.

(878)

Here we describe one DG ( S654A ) transgenic line where VIA4 - 1 antibody binding is absent in skeletal muscle.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9952	Here we describe one ProteinDG ( S654A ) transgenic line where VIA4 - 1 antibody binding is absent in skeletal muscle.

(879)

In theory, the absence of this carbohydrate antigen should inhibit later glycosylation events that would occur on the structure or structures this antibody binds to.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(880)

One such modification is likely to be the CT carbohydrate antigen, which is present on alpha dystroglycan in muscles overexpressing the CT GalNAc transferase [ Dev.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Text
0.8865	One such modification is likely to be the CT carbohydrate antigen, which is present on alpha dystroglycan in muscles overexpressing the ProteinCT GalNAc transferase [ Dev.
0.5733	One such modification is likely to be the CT carbohydrate antigen, which is present on alpha dystroglycan in muscles overexpressing the CT ProteinGalNAc transferase [ Dev.
0.5548	One such modification is likely to be the CT carbohydrate antigen, which is present on Proteinalpha dystroglycan in muscles overexpressing the CT GalNAc transferase [ Dev.

(881)

Biol. 242 ( 2002 ) 58 ].

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(882)

To test the relationship between the VIA4 - 1 and CT carbohydrate antigens, we made DG ( S654A ) / CT GalNAc transferase ( DG ( S654A ) / CT ) transgenic mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9634	To test the relationship between the VIA4 - 1 and CT carbohydrate antigens, we made DG ( S654A ) / CT GalNAc transferase Protein( DG ( S654A ) / CT ) transgenic mice.
0.9532	To test the relationship between the VIA4 - 1 and CT carbohydrate antigens, we made ProteinDG ( S654A ) / CT GalNAc transferase ( DG ( S654A ) / CT ) transgenic mice.
0.5933	To test the relationship between the VIA4 - 1 and CT carbohydrate antigens, we made ProteinDG ( S654A ) / CT GalNAc transferase ( DG ( S654A ) / CT ) transgenic mice.

(883)

Surprisingly, dystroglycan was cleaved, and alpha dystroglycan was glycosylated with the VIA4 - 1 antigen, in DG ( S654A ) / CT muscles.

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9874	Surprisingly, dystroglycan was cleaved, and alpha dystroglycan was glycosylated with the VIA4 - 1 antigen, in ProteinDG ( S654A ) / CT muscles.
0.9872	Surprisingly, Proteindystroglycan was cleaved, and alpha dystroglycan was glycosylated with the VIA4 - 1 antigen, in DG ( S654A ) / CT muscles.
0.9840	Surprisingly, dystroglycan was cleaved, and alpha dystroglycan was Glycosylationglycosylated with the VIA4 - 1 antigen, in DG ( S654A ) / CT muscles.
0.7953	Surprisingly, dystroglycan was cleaved, and Proteinalpha dystroglycan was glycosylated with the VIA4 - 1 antigen, in DG ( S654A ) / CT muscles.
0.7708	Surprisingly, dystroglycan was cleaved, and alpha Proteindystroglycan was glycosylated with the VIA4 - 1 antigen, in DG ( S654A ) / CT muscles.

(884)

In addition, muscles in DG ( S654A ) / CT transgenic mice had little or no evidence of muscular dystrophy when compared to DG ( S654A ) littermates.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	In addition, muscles in ProteinDG ( S654A ) / CT transgenic mice had little or no evidence of muscular dystrophy when compared to DG ( S654A ) littermates.
0.9957	In addition, muscles in DG ( S654A ) / CT transgenic mice had little or no evidence of muscular dystrophy when compared to ProteinDG ( S654A ) littermates.

(885)

These experiments demonstrate that the CT GalNAc transferase can affect the post - translational processing of dystroglycan and the extent of muscular dystrophy even in muscles where the VIA4 - 1 antigen is not present.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9948	These experiments demonstrate that the CT GalNAc transferase can affect the post - translational processing of Proteindystroglycan and the extent of muscular dystrophy even in muscles where the VIA4 - 1 antigen is not present.
0.9403	These experiments demonstrate that the ProteinCT GalNAc transferase can affect the post - translational processing of dystroglycan and the extent of muscular dystrophy even in muscles where the VIA4 - 1 antigen is not present.

(886)

Characterization of two novel proteins, NgRH1 and NgRH2, structurally and biochemically homologous to the Nogo - 66 receptor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	Characterization of two novel proteins, NgRH1 and ProteinNgRH2 , structurally and biochemically homologous to the Nogo - 66 receptor.
0.9993	Characterization of two novel proteins, ProteinNgRH1 and NgRH2, structurally and biochemically homologous to the Nogo - 66 receptor.
0.9235	Characterization of two novel proteins, NgRH1 and NgRH2, structurally and biochemically homologous to the ProteinNogo - 66 receptor .

(887)

Nogo - 66 receptor ( NgR ) has recently been identified as the neuronal receptor of the myelin - associated proteins Nogo - A, oligodendrocyte protein ( OMgp ) and myelin - associated glycoprotein ( MAG ), and mediates inhibition of axonal regeneration both in vitro and in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9952	Nogo - 66 receptor ( NgR ) has recently been identified as the neuronal receptor of the myelin - associated proteins ProteinNogo - A , oligodendrocyte protein ( OMgp ) and myelin - associated glycoprotein ( MAG ), and mediates inhibition of axonal regeneration both in vitro and in vivo.
0.9863	Nogo - 66 receptor Protein( NgR ) has recently been identified as the neuronal receptor of the myelin - associated proteins Nogo - A, oligodendrocyte protein ( OMgp ) and myelin - associated glycoprotein ( MAG ), and mediates inhibition of axonal regeneration both in vitro and in vivo.
0.9735	Nogo - 66 receptor ( NgR ) has recently been identified as the neuronal receptor of the myelin - associated proteins Nogo - A, oligodendrocyte protein Protein( OMgp ) and myelin - associated glycoprotein ( MAG ), and mediates inhibition of axonal regeneration both in vitro and in vivo.
0.9712	ProteinNogo - 66 receptor ( NgR ) has recently been identified as the neuronal receptor of the myelin - associated proteins Nogo - A, oligodendrocyte protein ( OMgp ) and myelin - associated glycoprotein ( MAG ), and mediates inhibition of axonal regeneration both in vitro and in vivo.
0.9659	Nogo - 66 receptor ( NgR ) has recently been identified as the neuronal receptor of the myelin - associated proteins Nogo - A, oligodendrocyte protein ( OMgp ) and myelin - associated glycoprotein Protein( MAG ) , and mediates inhibition of axonal regeneration both in vitro and in vivo.
0.9487	Nogo - 66 receptor ( NgR ) has recently been identified as the neuronal receptor of the myelin - associated proteins Nogo - A, Proteinoligodendrocyte protein ( OMgp ) and myelin - associated glycoprotein ( MAG ), and mediates inhibition of axonal regeneration both in vitro and in vivo.
0.8808	Nogo - 66 receptor ( NgR ) has recently been identified as the neuronal receptor of the myelin - associated proteins Nogo - A, oligodendrocyte protein ( OMgp ) and Proteinmyelin - associated glycoprotein ( MAG ), and mediates inhibition of axonal regeneration both in vitro and in vivo.

(888)

Through database searches, we have identified two novel proteins ( NgRH1 and NgRH2 ) that turned out to be homologous in their primary structures, biochemical properties and expression patterns to NgR.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Through database searches, we have identified two novel proteins ( NgRH1 and NgRH2 ) that turned out to be homologous in their primary structures, biochemical properties and expression patterns to ProteinNgR .
0.9997	Through database searches, we have identified two novel proteins ( NgRH1 and ProteinNgRH2 ) that turned out to be homologous in their primary structures, biochemical properties and expression patterns to NgR.
0.9988	Through database searches, we have identified two novel proteins Protein( NgRH1 and NgRH2 ) that turned out to be homologous in their primary structures, biochemical properties and expression patterns to NgR.

(889)

Like NgR, the homologues contain eight leucine - rich repeats ( LRR ) flanked by a leucine - rich repeat C - terminus ( LRRCT ) and a leucine - rich repeat N - terminus ( LRRNT ), and also have a C - terminal GPI signal sequence.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	Like ProteinNgR , the homologues contain eight leucine - rich repeats ( LRR ) flanked by a leucine - rich repeat C - terminus ( LRRCT ) and a leucine - rich repeat N - terminus ( LRRNT ), and also have a C - terminal GPI signal sequence.

(890)

Northern blot analysis showed predominant expression of NgRH1 and NgRH2 mRNA in the brain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Northern blot analysis showed predominant expression of NgRH1 and ProteinNgRH2 mRNA in the brain.
0.9996	Northern blot analysis showed predominant expression of ProteinNgRH1 and NgRH2 mRNA in the brain.

(891)

In situ hybridization and immunohistochemistry on rat brain slices revealed neuronal expression of the genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(892)

NgRH1 and NgRH2 were detected on the cell surface of recombinant cell lines as N - glycosylated GPI anchored proteins and, consistent with other GPI anchored proteins, were localized within the lipid rafts of cellular membranes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9990	NgRH1 and ProteinNgRH2 were detected on the cell surface of recombinant cell lines as N - glycosylated GPI anchored proteins and, consistent with other GPI anchored proteins, were localized within the lipid rafts of cellular membranes.
0.9985	ProteinNgRH1 and NgRH2 were detected on the cell surface of recombinant cell lines as N - glycosylated GPI anchored proteins and, consistent with other GPI anchored proteins, were localized within the lipid rafts of cellular membranes.
0.9899	NgRH1 and NgRH2 were detected on the cell surface of recombinant cell lines as GlycosylationN - glycosylated GPI anchored proteins and, consistent with other GPI anchored proteins, were localized within the lipid rafts of cellular membranes.

(893)

In addition, an N - terminal proteolytic fragment of NgR comprising the majority of the ectodomain was found to be constitutively secreted from cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	In addition, an N - terminal proteolytic fragment of ProteinNgR comprising the majority of the ectodomain was found to be constitutively secreted from cells.

(894)

Our data indicate that NgR, NgRH1 and NgRH2 constitute a novel receptor protein family, which may play related roles within the CNS.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	Our data indicate that NgR, NgRH1 and ProteinNgRH2 constitute a novel receptor protein family, which may play related roles within the CNS.
0.9986	Our data indicate that NgR, ProteinNgRH1 and NgRH2 constitute a novel receptor protein family, which may play related roles within the CNS.
0.9973	Our data indicate that ProteinNgR , NgRH1 and NgRH2 constitute a novel receptor protein family, which may play related roles within the CNS.

(895)

Synthesis of hydroxymethylglutathione from glutathione and L - serine catalyzed by carboxypeptidase Y.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9438	Synthesis of hydroxymethylglutathione from glutathione and L - serine catalyzed by Proteincarboxypeptidase Y .

(896)

Hydroxymethylglutathione ( gamma - L - glutamyl - L - cysteinyl - L - serine ; hmGSH ) occurs in many species belonging to the family Gramineae, but the biosynthetic pathway for hmGSH has not been identified.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(897)

We found that carboxypeptidase Y ( CPY ), but not carboxypeptidase A, catalyzed hmGSH synthesis from glutathione and L - serine in vitro at acidic pH.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9779	We found that carboxypeptidase Y Protein( CPY ) , but not carboxypeptidase A, catalyzed hmGSH synthesis from glutathione and L - serine in vitro at acidic pH.
0.8863	We found that Proteincarboxypeptidase Y ( CPY ), but not carboxypeptidase A, catalyzed hmGSH synthesis from glutathione and L - serine in vitro at acidic pH.
0.8455	We found that carboxypeptidase Y ( CPY ), but not Proteincarboxypeptidase A , catalyzed hmGSH synthesis from glutathione and L - serine in vitro at acidic pH.

(898)

CPY also catalyzed methylglutathione synthesis from glutathione and L - alanine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(899)

These findings suggested that a carboxypeptidase - like enzyme may be involved in hmGSH synthesis in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(900)

Transcriptional elongation by RNA polymerase II and histone methylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9975	Transcriptional elongation by RNA polymerase II and Proteinhistone methylation.
0.9516	Transcriptional elongation by RNA polymerase II and histone Methylationmethylation .

(901)

mRNA synthesis in eukaryotic organisms is a key biological process that is regulated at multiple levels.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(902)

From the covalent modifications of chromatin by a number of chromatin remodeling complexes during the initiation and activation steps of transcription to the processing of mRNA transcripts, a very large consortium of proteins and multiprotein complexes is critical for gene expression by RNA polymerase II.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(903)

The list of proteins essential for the successful synthesis of mRNA continues to grow at a rapid pace.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(904)

Recent advances in this area of research have been focused on transcription through chromatin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(905)

In this article, we will review the recent literature linking the key biochemical process of transcriptional elongation by RNA polymerase II to histone methylation by COMPASS, Dot1p, and Set2 methyltransferases.

Precision: 100.00

Recall: 80.00

F1: 88.89

Score	Text
0.9981	In this article, we will review the recent literature linking the key biochemical process of transcriptional elongation by RNA polymerase II to histone methylation by COMPASS, ProteinDot1p , and Set2 methyltransferases.
0.9981	In this article, we will review the recent literature linking the key biochemical process of transcriptional elongation by RNA polymerase II to Proteinhistone methylation by COMPASS, Dot1p, and Set2 methyltransferases.
0.9981	In this article, we will review the recent literature linking the key biochemical process of transcriptional elongation by RNA polymerase II to histone methylation by COMPASS, Dot1p, and ProteinSet2 methyltransferases.
0.5500	In this article, we will review the recent literature linking the key biochemical process of transcriptional elongation by RNA polymerase II to histone Methylationmethylation by COMPASS, Dot1p, and Set2 methyltransferases.
	In this article, we will review the recent literature linking the key biochemical process of transcriptional elongation by RNA polymerase II to histone Catalysismethylation by COMPASS, Dot1p, and Set2 methyltransferases.

(906)

Prolyl hydroxylation of collagen type I is required for efficient binding to integrin alpha 1 beta 1 and platelet glycoprotein VI but not to alpha 2 beta 1.

Precision: 38.46

Recall: 100.00

F1: 55.56

Score	Text
0.9353	Prolyl hydroxylation of collagen type I is required for efficient binding to integrin alpha 1 beta 1 and Proteinplatelet glycoprotein VI but not to alpha 2 beta 1.
0.7942	Prolyl hydroxylation of collagen type I is required for efficient binding to integrin alpha 1 beta 1 and platelet glycoprotein VI but not to alpha 2 Proteinbeta 1 .
0.7669	Prolyl hydroxylation of collagen type I is required for efficient binding to integrin alpha 1 beta 1 and platelet glycoprotein VI but not to Proteinalpha 2 beta 1.
0.7274	Prolyl hydroxylation of collagen type I is required for efficient binding to integrin alpha 1 Proteinbeta 1 and platelet glycoprotein VI but not to alpha 2 beta 1.
0.7192	Prolyl hydroxylation of collagen type I is required for efficient binding to Proteinintegrin alpha 1 beta 1 and platelet glycoprotein VI but not to alpha 2 beta 1.
0.9459	Prolyl hydroxylation of collagen type I is required for efficient binding to integrin alpha 1 beta 1 and platelet glycoprotein VI but not to Proteinalpha 2 beta 1 .
0.9447	Prolyl hydroxylation of collagen type I is required for efficient binding to Proteinintegrin alpha 1 beta 1 and platelet glycoprotein VI but not to alpha 2 beta 1.
0.7023	Prolyl hydroxylation of collagen type I is required for efficient binding to integrin alpha 1 beta 1 and platelet glycoprotein VI but not to Proteinalpha 2 beta 1.
0.6902	Prolyl hydroxylation of collagen type I is required for efficient binding to integrin alpha 1 beta 1 and platelet Proteinglycoprotein VI but not to alpha 2 beta 1.
0.6827	Prolyl hydroxylation of collagen type I is required for efficient binding to Proteinintegrin alpha 1 beta 1 and platelet glycoprotein VI but not to alpha 2 beta 1.
0.6570	Prolyl hydroxylation of collagen type I is required for efficient binding to integrin alpha 1 beta 1 and platelet glycoprotein VI but not to alpha Protein2 beta 1 .
0.5569	Prolyl hydroxylation of collagen type I is required for efficient binding to integrin alpha Protein1 beta 1 and platelet glycoprotein VI but not to alpha 2 beta 1.
0.5300	Prolyl hydroxylation of collagen type I is required for efficient binding to integrin Proteinalpha 1 beta 1 and platelet glycoprotein VI but not to alpha 2 beta 1.

(907)

Collagen is a potent adhesive substrate for cells, an event essentially mediated by the integrins alpha 1 beta 1 and alpha 2 beta 1.

Precision: 44.44

Recall: 100.00

F1: 61.54

Score	Text
0.8100	Collagen is a potent adhesive substrate for cells, an event essentially mediated by the integrins alpha 1 beta 1 and alpha 2 Proteinbeta 1 .
0.6564	Collagen is a potent adhesive substrate for cells, an event essentially mediated by the integrins alpha 1 beta 1 and Proteinalpha 2 beta 1.
0.6361	Collagen is a potent adhesive substrate for cells, an event essentially mediated by the Proteinintegrins alpha 1 beta 1 and alpha 2 beta 1.
0.6072	Collagen is a potent adhesive substrate for cells, an event essentially mediated by the integrins alpha 1 Proteinbeta 1 and alpha 2 beta 1.
0.9314	Collagen is a potent adhesive substrate for cells, an event essentially mediated by the integrins alpha 1 beta 1 and Proteinalpha 2 beta 1 .
0.9018	Collagen is a potent adhesive substrate for cells, an event essentially mediated by the Proteinintegrins alpha 1 beta 1 and alpha 2 beta 1.
0.6696	Collagen is a potent adhesive substrate for cells, an event essentially mediated by the integrins alpha 1 beta 1 and alpha Protein2 beta 1 .
0.5961	Collagen is a potent adhesive substrate for cells, an event essentially mediated by the Proteinintegrins alpha 1 beta 1 and alpha 2 beta 1.
0.5501	Collagen is a potent adhesive substrate for cells, an event essentially mediated by the integrins alpha 1 beta 1 and Proteinalpha 2 beta 1.

(908)

Collagen fibrils also bind to the integrin alpha 2 beta 1 and the platelet receptor glycoprotein VI to activate and aggregate platelets.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Text
0.8534	Collagen fibrils also bind to the integrin alpha 2 beta 1 and the Proteinplatelet receptor glycoprotein VI to activate and aggregate platelets.
0.8337	Collagen fibrils also bind to the Proteinintegrin alpha 2 beta 1 and the platelet receptor glycoprotein VI to activate and aggregate platelets.
0.7515	Collagen fibrils also bind to the integrin alpha 2 Proteinbeta 1 and the platelet receptor glycoprotein VI to activate and aggregate platelets.
0.9398	Collagen fibrils also bind to the Proteinintegrin alpha 2 beta 1 and the platelet receptor glycoprotein VI to activate and aggregate platelets.
0.8212	Collagen fibrils also bind to the Proteinintegrin alpha 2 beta 1 and the platelet receptor glycoprotein VI to activate and aggregate platelets.
0.6488	Collagen fibrils also bind to the integrin alpha Protein2 beta 1 and the platelet receptor glycoprotein VI to activate and aggregate platelets.
0.6096	Collagen fibrils also bind to the integrin alpha 2 beta 1 and the platelet receptor Proteinglycoprotein VI to activate and aggregate platelets.
0.5837	Collagen fibrils also bind to the integrin alpha 2 beta 1 and the platelet Proteinreceptor glycoprotein VI to activate and aggregate platelets.
0.5626	Collagen fibrils also bind to the integrin Proteinalpha 2 beta 1 and the platelet receptor glycoprotein VI to activate and aggregate platelets.

(909)

The distinct triple helical recognition motifs for these receptors, GXOGER and ( GPO ) n, respectively, all contain hydroxyproline.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(910)

Using unhydroxylated collagen I produced in transgenic plants, we investigated the role of hydroxyproline in the receptor - binding properties of collagen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(911)

We show that alpha 2 beta 1 but not alpha 1 beta 1 mediates cell adhesion to unhydroxylated collagen.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.7679	We show that alpha 2 Proteinbeta 1 but not alpha 1 beta 1 mediates cell adhesion to unhydroxylated collagen.
0.7187	We show that Proteinalpha 2 beta 1 but not alpha 1 beta 1 mediates cell adhesion to unhydroxylated collagen.
0.6411	We show that alpha 2 beta 1 but not alpha 1 Proteinbeta 1 mediates cell adhesion to unhydroxylated collagen.
0.6122	We show that alpha 2 beta 1 but not Proteinalpha 1 beta 1 mediates cell adhesion to unhydroxylated collagen.
0.9010	We show that Proteinalpha 2 beta 1 but not alpha 1 beta 1 mediates cell adhesion to unhydroxylated collagen.
0.8705	We show that alpha 2 beta 1 but not Proteinalpha 1 beta 1 mediates cell adhesion to unhydroxylated collagen.
0.6512	We show that alpha Protein2 beta 1 but not alpha 1 beta 1 mediates cell adhesion to unhydroxylated collagen.
0.6263	We show that Proteinalpha 2 beta 1 but not alpha 1 beta 1 mediates cell adhesion to unhydroxylated collagen.

(912)

Soluble recombinant alpha 1 beta 1 binding to unhydroxylated collagen is considerably reduced compared with bovine collagens, but binding can be restored by prolyl hydroxylation of recombinant collagen.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Text
0.8606	Soluble recombinant alpha 1 Proteinbeta 1 binding to unhydroxylated collagen is considerably reduced compared with bovine collagens, but binding can be restored by prolyl hydroxylation of recombinant collagen.
0.7338	Soluble recombinant Proteinalpha 1 beta 1 binding to unhydroxylated collagen is considerably reduced compared with bovine collagens, but binding can be restored by prolyl hydroxylation of recombinant collagen.
0.8975	Soluble recombinant Proteinalpha 1 beta 1 binding to unhydroxylated collagen is considerably reduced compared with bovine collagens, but binding can be restored by prolyl hydroxylation of recombinant collagen.
0.8135	Soluble recombinant alpha Protein1 beta 1 binding to unhydroxylated collagen is considerably reduced compared with bovine collagens, but binding can be restored by prolyl hydroxylation of recombinant collagen.
0.6925	Soluble recombinant Proteinalpha 1 beta 1 binding to unhydroxylated collagen is considerably reduced compared with bovine collagens, but binding can be restored by prolyl hydroxylation of recombinant collagen.
0.5248	Soluble recombinant alpha Protein1 beta 1 binding to unhydroxylated collagen is considerably reduced compared with bovine collagens, but binding can be restored by prolyl hydroxylation of recombinant collagen.

(913)

We also show that platelets use alpha 2 beta 1 to adhere to the unhydroxylated recombinant molecules, but the adhesion is weaker than on fully hydroxylated collagen, and the unhydroxylated collagen fibrils fail to aggregate platelets.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Text
0.9418	We also show that platelets use alpha 2 Proteinbeta 1 to adhere to the unhydroxylated recombinant molecules, but the adhesion is weaker than on fully hydroxylated collagen, and the unhydroxylated collagen fibrils fail to aggregate platelets.
0.8463	We also show that platelets use Proteinalpha 2 beta 1 to adhere to the unhydroxylated recombinant molecules, but the adhesion is weaker than on fully hydroxylated collagen, and the unhydroxylated collagen fibrils fail to aggregate platelets.
0.9671	We also show that platelets use Proteinalpha 2 beta 1 to adhere to the unhydroxylated recombinant molecules, but the adhesion is weaker than on fully hydroxylated collagen, and the unhydroxylated collagen fibrils fail to aggregate platelets.
0.9109	We also show that platelets use alpha Protein2 beta 1 to adhere to the unhydroxylated recombinant molecules, but the adhesion is weaker than on fully hydroxylated collagen, and the unhydroxylated collagen fibrils fail to aggregate platelets.
0.8095	We also show that platelets use Proteinalpha 2 beta 1 to adhere to the unhydroxylated recombinant molecules, but the adhesion is weaker than on fully hydroxylated collagen, and the unhydroxylated collagen fibrils fail to aggregate platelets.
0.6020	We also show that platelets use alpha Protein2 beta 1 to adhere to the unhydroxylated recombinant molecules, but the adhesion is weaker than on fully hydroxylated collagen, and the unhydroxylated collagen fibrils fail to aggregate platelets.

(914)

Prolyl hydroxylation is thus required for binding of collagen to platelet glycoprotein VI and to cells by alpha 1 beta 1.

Precision: 42.86

Recall: 100.00

F1: 60.00

Score	Text
0.9091	Prolyl hydroxylation is thus required for binding of collagen to Proteinplatelet glycoprotein VI and to cells by alpha 1 beta 1.
0.8324	Prolyl hydroxylation is thus required for binding of collagen to platelet glycoprotein VI and to cells by alpha 1 Proteinbeta 1 .
0.6804	Prolyl hydroxylation is thus required for binding of collagen to platelet glycoprotein VI and to cells by Proteinalpha 1 beta 1.
0.9264	Prolyl hydroxylation is thus required for binding of collagen to platelet glycoprotein VI and to cells by Proteinalpha 1 beta 1 .
0.7171	Prolyl hydroxylation is thus required for binding of collagen to platelet glycoprotein VI and to cells by alpha Protein1 beta 1 .
0.6073	Prolyl hydroxylation is thus required for binding of collagen to platelet glycoprotein VI and to cells by Proteinalpha 1 beta 1.
0.5952	Prolyl hydroxylation is thus required for binding of collagen to platelet Proteinglycoprotein VI and to cells by alpha 1 beta 1.

(915)

These observations give new insights into the molecular basis of collagen - receptor interactions and offer new selective applications for the recombinant unhydroxylated collagen I.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(916)

Mechanism, mutagenesis, and chemical rescue of a beta - mannosidase from cellulomonas fimi.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9920	Mechanism, mutagenesis, and chemical rescue of a Proteinbeta - mannosidase from cellulomonas fimi.

(917)

The chemical mechanism of a retaining beta - mannosidase from Cellulomonas fimi has been characterized through steady - state kinetic analyses with a range of substrates, coupled with chemical rescue studies on both the wild - type enzyme and mutants in which active site carboxyl groups have been replaced.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9940	The chemical mechanism of a retaining Proteinbeta - mannosidase from Cellulomonas fimi has been characterized through steady - state kinetic analyses with a range of substrates, coupled with chemical rescue studies on both the wild - type enzyme and mutants in which active site carboxyl groups have been replaced.

(918)

Studies with a series of aryl beta - mannosides of vastly different reactivities ( pK ( a ) ( lg ) = 4 - 10 ) allowed kinetic isolation of the glycosylation and deglycosylation steps.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(919)

Substrate inhibition was observed for all but the least reactive of these substrates.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(920)

Bronsted analysis of k ( cat ) revealed a downward breaking plot ( beta ( lg ) = - 0. 54 + / - 0. 05 ) that is consistent with a change in rate - determining step ( glycosylation to deglycosylation ), and this was confirmed by partitioning studies with ethylene glycol.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(921)

The pH dependence of k ( cat ) / K ( m ) follows an apparent single ionization of a group of pK ( a ) = 7. 65 that must be protonated for catalysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(922)

The tentative assignment of E429 as the acid - base catalyst of Man2A on the basis of sequence alignments with other family 2 glycosidases was confirmed by the increased turnover rate observed for the mutant E429A in the presence of azide and fluoride, leading to the production of beta - mannosyl azide and beta - mannosyl fluoride, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9971	The tentative assignment of E429 as the acid - base catalyst of ProteinMan2A on the basis of sequence alignments with other family 2 glycosidases was confirmed by the increased turnover rate observed for the mutant E429A in the presence of azide and fluoride, leading to the production of beta - mannosyl azide and beta - mannosyl fluoride, respectively.

(923)

A pH - dependent chemical rescue of E429A activity is also observed with citrate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(924)

Substantial oxocarbenium ion character at the transition state was demonstrated by the alpha - deuterium kinetic isotope effect for Man2A E429A of alpha - D ( V ) = 1. 12 + / - 0. 01.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9968	Substantial oxocarbenium ion character at the transition state was demonstrated by the alpha - deuterium kinetic isotope effect for ProteinMan2A E429A of alpha - D ( V ) = 1. 12 + / - 0. 01.

(925)

Surprisingly, this isotope effect was substantially greater in the presence of azide ( alpha - D ( V ) = 1. 166 + / - 0. 009 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(926)

Likely involvement of acid / base catalysis was revealed by the pH dependence of k ( cat ) for Man2A E429A, which follows a bell - shaped profile described by pK ( a ) values of 6. 1 and 8. 4, substantially different from that of the wild - type enzyme.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9856	Likely involvement of acid / base catalysis was revealed by the pH dependence of k ( cat ) for ProteinMan2A E429A, which follows a bell - shaped profile described by pK ( a ) values of 6. 1 and 8. 4, substantially different from that of the wild - type enzyme.

(927)

The glycosidic bond cleaving activity of Man2A E519A and E519S nucleophile mutants is restored with azide and fluoride and appears to correlate with the corresponding " glycosynthase " activities.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9930	The glycosidic bond cleaving activity of ProteinMan2A E519A and E519S nucleophile mutants is restored with azide and fluoride and appears to correlate with the corresponding " glycosynthase " activities.

(928)

The contribution of the substrate 2 - hydroxyl to stabilization of the Man2A glycosylation transition state ( DeltaDeltaG ( ) = 5. 1 kcal mol ( - 1 ) ) was probed using a 2 - deoxymannose substrate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9983	The contribution of the substrate 2 - hydroxyl to stabilization of the ProteinMan2A glycosylation transition state ( DeltaDeltaG ( ) = 5. 1 kcal mol ( - 1 ) ) was probed using a 2 - deoxymannose substrate.

(929)

This value, surprisingly, is comparable to that found from equivalent studies with beta - glucosidases despite the geometric differences at C - 2 and the importance of hydrogen bonding at that position.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.7864	This value, surprisingly, is comparable to that found from equivalent studies with Proteinbeta - glucosidases despite the geometric differences at C - 2 and the importance of hydrogen bonding at that position.

(930)

Modes of stabilizing the mannosidase transition state are discussed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(931)

Occurrence of O - linked Xyl - GlcNAc and Xyl - Glc disaccharides in trocarin, a factor Xa homolog from snake venom.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9896	Occurrence of O - linked Xyl - GlcNAc and EntityXyl - Glc disaccharides in trocarin, a factor Xa homolog from snake venom.
0.9890	Occurrence of O - linked EntityXyl - GlcNAc and Xyl - Glc disaccharides in trocarin, a factor Xa homolog from snake venom.
0.9839	Occurrence of O - linked Xyl - GlcNAc and Xyl - Glc disaccharides in Proteintrocarin , a factor Xa homolog from snake venom.
0.9321	Occurrence of O - linked Xyl - GlcNAc and Xyl - Glc disaccharides in trocarin, a Proteinfactor Xa homolog from snake venom.
0.7677	Occurrence of GlycosylationO - linked Xyl - GlcNAc and Xyl - Glc disaccharides in trocarin, a factor Xa homolog from snake venom.

(932)

Trocarin is a 46515 - Da group D prothrombin - activating glycoprotein from the venom of the Australian elapid, Tropidechis carinatus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	ProteinTrocarin is a 46515 - Da group D prothrombin - activating glycoprotein from the venom of the Australian elapid, Tropidechis carinatus.
0.9932	Trocarin is a 46515 - Da group D Proteinprothrombin - activating glycoprotein from the venom of the Australian elapid, Tropidechis carinatus.

(933)

Amino acid sequencing and functional characterization of trocarin demonstrated that it is a structural and functional homolog of mammalian blood coagulation factor ( F ) Xa.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9985	Amino acid sequencing and functional characterization of Proteintrocarin demonstrated that it is a structural and functional homolog of mammalian blood coagulation factor ( F ) Xa.
0.6913	Amino acid sequencing and functional characterization of trocarin demonstrated that it is a structural and functional homolog of mammalian blood Proteincoagulation factor ( F ) Xa .

(934)

In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its heavy chain.

Precision: 52.94

Recall: 100.00

F1: 69.23

Score	Text
0.9912	In this study we show that, in contrast to mammalian Xa, which is not Glycosylationglycosylated , trocarin contains an O - linked carbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its heavy chain.
0.9650	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, Proteintrocarin contains an O - linked carbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its heavy chain.
0.9144	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an GlycosylationO - linked carbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its heavy chain.
0.8990	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its light chain and an GlycosylationN - linked carbohydrate oligosaccharide in its heavy chain.
0.8868	In this study we show that, in contrast to mammalian ProteinXa , which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its heavy chain.
0.7687	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its Entityheavy chain .
0.7522	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its light chain and an N - linked Entitycarbohydrate oligosaccharide in its heavy chain.
0.6818	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its Entitylight chain and an N - linked carbohydrate oligosaccharide in its heavy chain.
0.6305	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked Entitycarbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its heavy chain.
0.8622	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its light chain and an N - linked carbohydrate Entityoligosaccharide in its heavy chain.
0.8159	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its light Entitychain and an N - linked carbohydrate oligosaccharide in its heavy chain.
0.8087	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its heavy Entitychain .
0.7069	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its Entityheavy chain.
0.6974	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate Entitymoiety in its light chain and an N - linked carbohydrate oligosaccharide in its heavy chain.
0.6749	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its light chain and an N - linked Entitycarbohydrate oligosaccharide in its heavy chain.
0.5696	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked Entitycarbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its heavy chain.
0.5134	In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its Entitylight chain and an N - linked carbohydrate oligosaccharide in its heavy chain.

(935)

Mass spectrometry and sugar compositional analysis indicate that the O - linked carbohydrate moiety is a mixture of Xyl - GlcNAc -, GlcNAc -, Xyl - Glc - and Glc - structures linked to Ser 52.

Precision: 85.71

Recall: 100.00

F1: 92.31

Score	Text
0.9855	Mass spectrometry and sugar compositional analysis indicate that the O - linked carbohydrate moiety is a mixture of Xyl - GlcNAc -, EntityGlcNAc - , Xyl - Glc - and Glc - structures linked to Ser 52.
0.9813	Mass spectrometry and sugar compositional analysis indicate that the O - linked carbohydrate moiety is a mixture of Xyl - GlcNAc -, GlcNAc -, EntityXyl - Glc - and Glc - structures linked to Ser 52.
0.9705	Mass spectrometry and sugar compositional analysis indicate that the O - linked carbohydrate moiety is a mixture of EntityXyl - GlcNAc - , GlcNAc -, Xyl - Glc - and Glc - structures linked to Ser 52.
0.9619	Mass spectrometry and sugar compositional analysis indicate that the O - linked carbohydrate moiety is a mixture of Xyl - GlcNAc -, GlcNAc -, Xyl - Glc - and EntityGlc - structures linked to Ser 52.
0.9477	Mass spectrometry and sugar compositional analysis indicate that the GlycosylationO - linked carbohydrate moiety is a mixture of Xyl - GlcNAc -, GlcNAc -, Xyl - Glc - and Glc - structures linked to Ser 52.
0.8243	Mass spectrometry and sugar compositional analysis indicate that the O - linked carbohydrate moiety is a mixture of Xyl - GlcNAc -, GlcNAc -, Xyl - Glc - and Glc - structures linked to EntitySer 52 .
0.7969	Mass spectrometry and sugar compositional analysis indicate that the O - linked carbohydrate moiety is a mixture of Xyl - GlcNAc -, GlcNAc -, Xyl - Glc - and EntityGlc - structures linked to Ser 52.

(936)

The N - linked carbohydrate on Asn 45 of the heavy chain is a sialylated, diantennary oligosaccharide that is located at the lip of the active site of the prothrombin activator.

Precision: 50.00

Recall: 75.00

F1: 60.00

Score	Text
0.9811	The N - linked carbohydrate on Asn 45 of the heavy chain is a sialylated, diantennary oligosaccharide that is located at the lip of the active site of the Proteinprothrombin activator.
0.9423	The GlycosylationN - linked carbohydrate on Asn 45 of the heavy chain is a sialylated, diantennary oligosaccharide that is located at the lip of the active site of the prothrombin activator.
0.9020	The N - linked carbohydrate on EntityAsn 45 of the heavy chain is a sialylated, diantennary oligosaccharide that is located at the lip of the active site of the prothrombin activator.
0.7438	The N - linked Entitycarbohydrate on Asn 45 of the heavy chain is a sialylated, diantennary oligosaccharide that is located at the lip of the active site of the prothrombin activator.
0.5997	The N - linked carbohydrate on Asn 45 of the heavy chain is a sialylated, diantennary Entityoligosaccharide that is located at the lip of the active site of the prothrombin activator.
0.5575	The N - linked carbohydrate on EntityAsn 45 of the heavy chain is a sialylated, diantennary oligosaccharide that is located at the lip of the active site of the prothrombin activator.
	The N - linked carbohydrate on Asn 45 of the heavy chain is a Entitysialylated, diantennary oligosaccharide that is located at the lip of the active site of the prothrombin activator.

(937)

Differential glycosylation regulates processing of lipoprotein receptors by gamma - secretase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(938)

The low density lipoprotein ( LDL ) receptor - related protein 1 ( LRP1 ) belongs to a growing number of cell surface proteins that undergo regulated proteolytic processing that culminates in the release of their intracellular domain ( ICD ) by the intramembranous protease gamma - secretase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9825	The low density lipoprotein ( LDL ) receptor - related protein 1 Protein( LRP1 ) belongs to a growing number of cell surface proteins that undergo regulated proteolytic processing that culminates in the release of their intracellular domain ( ICD ) by the intramembranous protease gamma - secretase.
0.5787	The Proteinlow density lipoprotein ( LDL ) receptor - related protein 1 ( LRP1 ) belongs to a growing number of cell surface proteins that undergo regulated proteolytic processing that culminates in the release of their intracellular domain ( ICD ) by the intramembranous protease gamma - secretase.

(939)

Here we show that LRP1 is differentially glycosylated in a tissue - specific manner and that carbohydrate addition reduces proteolytic cleavage of the extracellular domain and, concomitantly, ICD release.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9970	Here we show that ProteinLRP1 is differentially glycosylated in a tissue - specific manner and that carbohydrate addition reduces proteolytic cleavage of the extracellular domain and, concomitantly, ICD release.
0.9955	Here we show that LRP1 is differentially Glycosylationglycosylated in a tissue - specific manner and that carbohydrate addition reduces proteolytic cleavage of the extracellular domain and, concomitantly, ICD release.

(940)

The apolipoprotein E ( apoE ) receptor - 2 ( apoER2 ), another member of the LDL receptor family with functions in cellular signal transmission, also undergoes sequential proteolytic processing, resulting in intracellular domain release into the cytoplasm.

Precision: 100.00

Recall: 50.00

F1: 66.67

Score	Text
0.9797	The apolipoprotein E ( apoE ) receptor - 2 Protein( apoER2 ) , another member of the LDL receptor family with functions in cellular signal transmission, also undergoes sequential proteolytic processing, resulting in intracellular domain release into the cytoplasm.
	The Proteinapolipoprotein E ( apoE ) receptor - 2 ( apoER2 ), another member of the LDL receptor family with functions in cellular signal transmission, also undergoes sequential proteolytic processing, resulting in intracellular domain release into the cytoplasm.

(941)

The penultimate processing step also involves cleavage of the apoER2 extracellular domain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9980	The penultimate processing step also involves cleavage of the ProteinapoER2 extracellular domain.

(942)

The rate at which this cleavage step occurs is determined by the glycosylation state of the receptor, which in turn is regulated by the alternative splicing of an exon encoding several O - linked sugar attachment sites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9695	The rate at which this cleavage step occurs is determined by the Glycosylationglycosylation state of the receptor, which in turn is regulated by the alternative splicing of an exon encoding several O - linked sugar attachment sites.

(943)

These findings suggest a role for differential and tissue - specific glycosylation as a physiological switch that modulates the diverse biological functions of these receptors in a cell - type specific manner.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(944)

Inactivation of p16 gene in leukemia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Inactivation of Proteinp16 gene in leukemia.

(945)

To determine the frequency of p16 gene inactivation in leukemia cells, and to evaluate their value in the prediction of their clinical outcome.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	To determine the frequency of Proteinp16 gene inactivation in leukemia cells, and to evaluate their value in the prediction of their clinical outcome.

(946)

Bone marrow or peripheral blood samples from 48 patients with leukemia were examined by multiplex polymerase chain reaction ( MPCR ) to detect p16 gene homozygous deletion, and restriction enzyme PCR to detect p16 gene methylation. p16 gene inactivation were detected in 10 of the 48 patients ( 20. 4 % ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9990	Bone marrow or peripheral blood samples from 48 patients with leukemia were examined by multiplex polymerase chain reaction ( MPCR ) to detect p16 gene homozygous deletion, and restriction enzyme PCR to detect Proteinp16 gene methylation. p16 gene inactivation were detected in 10 of the 48 patients ( 20. 4 % ).
0.9989	Bone marrow or peripheral blood samples from 48 patients with leukemia were examined by multiplex polymerase chain reaction ( MPCR ) to detect Proteinp16 gene homozygous deletion, and restriction enzyme PCR to detect p16 gene methylation. p16 gene inactivation were detected in 10 of the 48 patients ( 20. 4 % ).
0.9989	Bone marrow or peripheral blood samples from 48 patients with leukemia were examined by multiplex polymerase chain reaction ( MPCR ) to detect p16 gene homozygous deletion, and restriction enzyme PCR to detect p16 gene methylation. Proteinp16 gene inactivation were detected in 10 of the 48 patients ( 20. 4 % ).
0.9972	Bone marrow or peripheral blood samples from 48 patients with leukemia were examined by multiplex polymerase chain reaction ( MPCR ) to detect p16 gene homozygous deletion, and restriction enzyme PCR to detect p16 gene DNA_methylationmethylation . p16 gene inactivation were detected in 10 of the 48 patients ( 20. 4 % ).

(947)

They were five patients with p16 homozygous deletion, and five patients with p16 methylation, respectively. p16 gene inactivation correlates with adverse prognosis features.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	They were five patients with Proteinp16 homozygous deletion, and five patients with p16 methylation, respectively. p16 gene inactivation correlates with adverse prognosis features.
0.9981	They were five patients with p16 homozygous deletion, and five patients with p16 methylation, respectively. Proteinp16 gene inactivation correlates with adverse prognosis features.
0.9966	They were five patients with p16 homozygous deletion, and five patients with Proteinp16 methylation, respectively. p16 gene inactivation correlates with adverse prognosis features.
0.9877	They were five patients with p16 homozygous deletion, and five patients with p16 DNA_methylationmethylation , respectively. p16 gene inactivation correlates with adverse prognosis features.

(948)

The patients with p16 inactivation had poor response to chemotherapy, and had significantly shorter survival times than the patients in whom p16 gene was preserved ( P < 0. 001 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	The patients with p16 inactivation had poor response to chemotherapy, and had significantly shorter survival times than the patients in whom Proteinp16 gene was preserved ( P < 0. 001 ).
0.9998	The patients with Proteinp16 inactivation had poor response to chemotherapy, and had significantly shorter survival times than the patients in whom p16 gene was preserved ( P < 0. 001 ).

(949)

The inactivation of p16 gene play a key role in the pathogenesis and the progression of some leukemia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	The inactivation of Proteinp16 gene play a key role in the pathogenesis and the progression of some leukemia.

(950)

The detection of p16 gene is reliable prognostic factor that predict shortened survival times.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	The detection of Proteinp16 gene is reliable prognostic factor that predict shortened survival times.

(951)

A haploid affair : core histone transitions during spermatogenesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9955	A haploid affair : core Proteinhistone transitions during spermatogenesis.

(952)

The process of meiosis reduces a diploid cell to four haploid gametes and is accompanied by extensive recombination.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(953)

Thus, the dynamics of chromatin during meiosis are significantly different than in mitotic cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(954)

As spermatogenesis progresses, there is a widespread reorganization of the haploid genome followed by extensive DNA compaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(955)

It has become increasingly clear that the dynamic composition of chromatin plays a critical role in the activities of enzymes and processes that act upon it.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(956)

Therefore, an analysis of the role of histone variants and modifications in these processes may shed light upon the mechanisms involved and the control of chromatin structure in general.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9968	Therefore, an analysis of the role of Proteinhistone variants and modifications in these processes may shed light upon the mechanisms involved and the control of chromatin structure in general.

(957)

Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9946	Histone variants such as histone H3. 3, ProteinH2AX , and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.9895	Histone variants such as histone H3. 3, H2AX, and ProteinmacroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.9845	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of Proteinhistones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.9843	ProteinHistone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.9743	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, ProteinuH2B ) , and phosphorylation of histone H3 ( H3p ).
0.9689	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of Proteinhistone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.9676	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and ProteinH2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.9651	Histone variants such as Proteinhistone H3. 3 , H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.9373	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), Ubiquitinationubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.9341	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B Protein( uH2A , uH2B ), and phosphorylation of histone H3 ( H3p ).
0.9341	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and Phosphorylationphosphorylation of histone H3 ( H3p ).
0.9327	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 Protein( acH4 ) , ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.9241	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 Protein( H3p ) .
0.9062	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of Proteinhistone H3 ( H3p ).
0.8937	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated Acetylationacetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.9158	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and ProteinH2B ( uH2A , uH2B ), and phosphorylation of histone H3 ( H3p ).
0.9029	Histone variants such as Proteinhistone H3 . 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.7177	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of Proteinhistones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.6610	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones ProteinH2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).
0.6304	Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of Proteinhistone H4 ( acH4 ) , ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).

(958)

This review will examine recent discoveries concerning the role of histone modifications and variants during meiosis and spermatogenesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9976	This review will examine recent discoveries concerning the role of Proteinhistone modifications and variants during meiosis and spermatogenesis.

(959)

HIF prolyl - hydroxylase 2 is the key oxygen sensor setting low steady - state levels of HIF - 1alpha in normoxia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9982	HIF prolyl - hydroxylase 2 is the key oxygen sensor setting low steady - state levels of ProteinHIF - 1alpha in normoxia.
0.8177	ProteinHIF prolyl - hydroxylase 2 is the key oxygen sensor setting low steady - state levels of HIF - 1alpha in normoxia.

(960)

Hypoxia - inducible factor ( HIF ), a transcriptional complex conserved from Caenorhabditis elegans to vertebrates, plays a pivotal role in cellular adaptation to low oxygen availability.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(961)

In normoxia, the HIF - alpha subunits are targeted for destruction by prolyl hydroxylation, a specific modification that provides recognition for the E3 ubiquitin ligase complex containing the von Hippel - Lindau tumour suppressor protein ( pVHL ).

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Text
0.9855	In normoxia, the HIF - alpha subunits are targeted for destruction by prolyl hydroxylation, a specific modification that provides recognition for the E3 Proteinubiquitin ligase complex containing the von Hippel - Lindau tumour suppressor protein ( pVHL ).
0.9764	In normoxia, the HIF - alpha subunits are targeted for destruction by prolyl hydroxylation, a specific modification that provides recognition for the E3 ubiquitin ligase complex containing the von Hippel - Lindau tumour suppressor protein Protein( pVHL ) .
0.8452	In normoxia, the HIF - alpha subunits are targeted for destruction by prolyl hydroxylation, a specific modification that provides recognition for the E3 ubiquitin ligase complex containing the Proteinvon Hippel - Lindau tumour suppressor protein ( pVHL ).
0.7291	In normoxia, the HIF - alpha subunits are targeted for destruction by prolyl hydroxylation, a specific modification that provides recognition for the E3 ubiquitin ligase complex containing the von ProteinHippel - Lindau tumour suppressor protein ( pVHL ).
0.6931	In normoxia, the HIF - alpha subunits are targeted for destruction by prolyl hydroxylation, a specific modification that provides recognition for the E3 ubiquitin ligase complex containing the von Hippel - Lindau Proteintumour suppressor protein ( pVHL ).

(962)

Three HIF prolyl - hydroxylases ( PHD1, 2 and 3 ) were identified recently in mammals and shown to hydroxylate HIF - alpha subunits.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9990	Three HIF prolyl - hydroxylases ( PHD1, Protein2 and 3 ) were identified recently in mammals and shown to hydroxylate HIF - alpha subunits.
0.9976	Three HIF prolyl - hydroxylases ( PHD1, 2 and Protein3 ) were identified recently in mammals and shown to hydroxylate HIF - alpha subunits.
0.9942	Three HIF prolyl - hydroxylases Protein( PHD1 , 2 and 3 ) were identified recently in mammals and shown to hydroxylate HIF - alpha subunits.

(963)

Here we show that specific'silencing'of PHD2 with short interfering RNAs is sufficient to stabilize and activate HIF - 1alpha in normoxia in all the human cells investigated.'Silencing'of PHD1 and PHD3 has no effect on the stability of HIF - 1alpha either in normoxia or upon re - oxygenation of cells briefly exposed to hypoxia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Here we show that specific'silencing'of ProteinPHD2 with short interfering RNAs is sufficient to stabilize and activate HIF - 1alpha in normoxia in all the human cells investigated.'Silencing'of PHD1 and PHD3 has no effect on the stability of HIF - 1alpha either in normoxia or upon re - oxygenation of cells briefly exposed to hypoxia.
0.9998	Here we show that specific'silencing'of PHD2 with short interfering RNAs is sufficient to stabilize and activate HIF - 1alpha in normoxia in all the human cells investigated.'Silencing'of PHD1 and ProteinPHD3 has no effect on the stability of HIF - 1alpha either in normoxia or upon re - oxygenation of cells briefly exposed to hypoxia.
0.9998	Here we show that specific'silencing'of PHD2 with short interfering RNAs is sufficient to stabilize and activate HIF - 1alpha in normoxia in all the human cells investigated.'Silencing'of ProteinPHD1 and PHD3 has no effect on the stability of HIF - 1alpha either in normoxia or upon re - oxygenation of cells briefly exposed to hypoxia.
0.9995	Here we show that specific'silencing'of PHD2 with short interfering RNAs is sufficient to stabilize and activate HIF - 1alpha in normoxia in all the human cells investigated.'Silencing'of PHD1 and PHD3 has no effect on the stability of ProteinHIF - 1alpha either in normoxia or upon re - oxygenation of cells briefly exposed to hypoxia.
0.9994	Here we show that specific'silencing'of PHD2 with short interfering RNAs is sufficient to stabilize and activate ProteinHIF - 1alpha in normoxia in all the human cells investigated.'Silencing'of PHD1 and PHD3 has no effect on the stability of HIF - 1alpha either in normoxia or upon re - oxygenation of cells briefly exposed to hypoxia.

(964)

We therefore conclude that, in vivo, PHDs have distinct assigned functions, PHD2 being the critical oxygen sensor setting the low steady - state levels of HIF - 1alpha in normoxia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	We therefore conclude that, in vivo, PHDs have distinct assigned functions, ProteinPHD2 being the critical oxygen sensor setting the low steady - state levels of HIF - 1alpha in normoxia.
0.9993	We therefore conclude that, in vivo, PHDs have distinct assigned functions, PHD2 being the critical oxygen sensor setting the low steady - state levels of ProteinHIF - 1alpha in normoxia.

(965)

Interestingly, PHD2 is upregulated by hypoxia, providing an HIF - 1 - dependent auto - regulatory mechanism driven by the oxygen tension.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Interestingly, ProteinPHD2 is upregulated by hypoxia, providing an HIF - 1 - dependent auto - regulatory mechanism driven by the oxygen tension.

(966)

Insulation of the chicken beta - globin chromosomal domain from a chromatin - condensing protein, MENT.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	Insulation of the chicken beta - globin chromosomal domain from a chromatin - condensing protein, ProteinMENT .
0.9720	Insulation of the chicken Proteinbeta - globin chromosomal domain from a chromatin - condensing protein, MENT.

(967)

Active genes are insulated from developmentally regulated chromatin condensation in terminally differentiated cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(968)

We mapped the topography of a terminal stage - specific chromatin - condensing protein, MENT, across the active chicken beta - globin domain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	We mapped the topography of a terminal stage - specific chromatin - condensing protein, ProteinMENT , across the active chicken beta - globin domain.
0.9894	We mapped the topography of a terminal stage - specific chromatin - condensing protein, MENT, across the active chicken Proteinbeta - globin domain.

(969)

We observed two sharp transitions of MENT concentration coinciding with the beta - globin boundary elements.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9990	We observed two sharp transitions of ProteinMENT concentration coinciding with the beta - globin boundary elements.
0.9971	We observed two sharp transitions of MENT concentration coinciding with the Proteinbeta - globin boundary elements.

(970)

The MENT distribution profile was opposite to that of acetylated core histones but correlated with that of histone H3 dimethylated at lysine 9 ( H3me2K9 ).

Precision: 100.00

Recall: 87.50

F1: 93.33

Score	Text
0.9977	The ProteinMENT distribution profile was opposite to that of acetylated core histones but correlated with that of histone H3 dimethylated at lysine 9 ( H3me2K9 ).
0.9802	The MENT distribution profile was opposite to that of acetylated core Proteinhistones but correlated with that of histone H3 dimethylated at lysine 9 ( H3me2K9 ).
0.9703	The MENT distribution profile was opposite to that of Acetylationacetylated core histones but correlated with that of histone H3 dimethylated at lysine 9 ( H3me2K9 ).
0.9696	The MENT distribution profile was opposite to that of acetylated core histones but correlated with that of histone H3 Methylationdimethylated at lysine 9 ( H3me2K9 ).
0.9544	The MENT distribution profile was opposite to that of acetylated core histones but correlated with that of Proteinhistone H3 dimethylated at lysine 9 ( H3me2K9 ).
0.8819	The MENT distribution profile was opposite to that of acetylated core histones but correlated with that of histone H3 dimethylated at Entitylysine 9 ( H3me2K9 ).
0.5393	The MENT distribution profile was opposite to that of acetylated core histones but correlated with that of histone H3 dimethylated at lysine 9 Entity( H3me2K9 ) .
	The MENT distribution profile was opposite to that of acetylated core histones but correlated with that of histone H3 dimethylated at lysine 9 Protein( H3me2K9 ) .

(971)

Ectopic MENT expression in NIH 3T3 cells caused a large - scale and specific remodeling of chromatin marked by H3me2K9.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	Ectopic ProteinMENT expression in NIH 3T3 cells caused a large - scale and specific remodeling of chromatin marked by H3me2K9.

(972)

MENT colocalized with H3me2K9 both in chicken erythrocytes and NIH 3T3 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9990	ProteinMENT colocalized with H3me2K9 both in chicken erythrocytes and NIH 3T3 cells.
0.9532	MENT colocalized with ProteinH3me2K9 both in chicken erythrocytes and NIH 3T3 cells.

(973)

Mutational analysis of MENT and experiments with deacetylase inhibitors revealed the essential role of the reaction center loop domain and an inhibitory affect of histone hyperacetylation on the MENT - induced chromatin remodeling in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	Mutational analysis of ProteinMENT and experiments with deacetylase inhibitors revealed the essential role of the reaction center loop domain and an inhibitory affect of histone hyperacetylation on the MENT - induced chromatin remodeling in vivo.
0.9899	Mutational analysis of MENT and experiments with deacetylase inhibitors revealed the essential role of the reaction center loop domain and an inhibitory affect of histone hyperacetylation on the ProteinMENT - induced chromatin remodeling in vivo.
0.9848	Mutational analysis of MENT and experiments with deacetylase inhibitors revealed the essential role of the reaction center loop domain and an inhibitory affect of Proteinhistone hyperacetylation on the MENT - induced chromatin remodeling in vivo.
0.9657	Mutational analysis of MENT and experiments with deacetylase inhibitors revealed the essential role of the reaction center loop domain and an inhibitory affect of histone Acetylationhyperacetylation on the MENT - induced chromatin remodeling in vivo.

(974)

In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with dimethylated but not acetylated N - terminal domain of histone H3 specifically restored chromatin self - association by MENT.

Precision: 77.78

Recall: 100.00

F1: 87.50

Score	Text
0.9971	In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by ProteinMENT , while reconstitution with dimethylated but not acetylated N - terminal domain of histone H3 specifically restored chromatin self - association by MENT.
0.9966	In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with dimethylated but not acetylated N - terminal domain of histone H3 specifically restored chromatin self - association by ProteinMENT .
0.9753	In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with dimethylated but not acetylated N - terminal domain of Proteinhistone H3 specifically restored chromatin self - association by MENT.
0.9649	In vitro, the elimination of the Proteinhistone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with dimethylated but not acetylated N - terminal domain of histone H3 specifically restored chromatin self - association by MENT.
0.9461	In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with Methylationdimethylated but not acetylated N - terminal domain of histone H3 specifically restored chromatin self - association by MENT.
0.9369	In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with dimethylated but not Acetylationacetylated N - terminal domain of histone H3 specifically restored chromatin self - association by MENT.
0.7865	In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with dimethylated but not acetylated EntityN - terminal domain of histone H3 specifically restored chromatin self - association by MENT.
0.6977	In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with dimethylated but not acetylated EntityN - terminal domain of histone H3 specifically restored chromatin self - association by MENT.
0.6568	In vitro, the elimination of the histone H3 N - terminal peptide containing Entitylysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with dimethylated but not acetylated N - terminal domain of histone H3 specifically restored chromatin self - association by MENT.

(975)

We suggest that histone H3 modification at lysine 9 directly regulates chromatin condensation by recruiting MENT to chromatin in a fashion that is spatially constrained from active genes by gene boundary elements and histone hyperacetylation.

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9996	We suggest that histone H3 modification at lysine 9 directly regulates chromatin condensation by recruiting ProteinMENT to chromatin in a fashion that is spatially constrained from active genes by gene boundary elements and histone hyperacetylation.
0.9942	We suggest that histone H3 modification at lysine 9 directly regulates chromatin condensation by recruiting MENT to chromatin in a fashion that is spatially constrained from active genes by gene boundary elements and Proteinhistone hyperacetylation.
0.9781	We suggest that Proteinhistone H3 modification at lysine 9 directly regulates chromatin condensation by recruiting MENT to chromatin in a fashion that is spatially constrained from active genes by gene boundary elements and histone hyperacetylation.
0.9725	We suggest that histone H3 modification at lysine 9 directly regulates chromatin condensation by recruiting MENT to chromatin in a fashion that is spatially constrained from active genes by gene boundary elements and histone Acetylationhyperacetylation .
0.5107	We suggest that histone H3 modification at Entitylysine 9 directly regulates chromatin condensation by recruiting MENT to chromatin in a fashion that is spatially constrained from active genes by gene boundary elements and histone hyperacetylation.

(976)

Requirement for neo1p in retrograde transport from the Golgi complex to the endoplasmic reticulum.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Requirement for Proteinneo1p in retrograde transport from the Golgi complex to the endoplasmic reticulum.

(977)

Neo1p from Saccharomyces cerevisiae is an essential P - type ATPase and potential aminophospholipid translocase ( flippase ) in the Drs2p family.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	ProteinNeo1p from Saccharomyces cerevisiae is an essential P - type ATPase and potential aminophospholipid translocase ( flippase ) in the Drs2p family.
0.9971	Neo1p from Saccharomyces cerevisiae is an essential P - type ATPase and potential aminophospholipid translocase ( flippase ) in the ProteinDrs2p family.

(978)

We have previously implicated Drs2p in protein transport steps in the late secretory pathway requiring ADP - ribosylation factor ( ARF ) and clathrin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	We have previously implicated ProteinDrs2p in protein transport steps in the late secretory pathway requiring ADP - ribosylation factor ( ARF ) and clathrin.

(979)

Here, we present evidence that epitope - tagged Neo1p localizes to the endoplasmic reticulum ( ER ) and Golgi complex and is required for a retrograde transport pathway between these organelles.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	Here, we present evidence that epitope - tagged ProteinNeo1p localizes to the endoplasmic reticulum ( ER ) and Golgi complex and is required for a retrograde transport pathway between these organelles.

(980)

Using conditional alleles of NEO1, we find that loss of Neo1p function causes cargo - specific defects in anterograde protein transport early in the secretory pathway and perturbs glycosylation in the Golgi complex.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Using conditional alleles of ProteinNEO1 , we find that loss of Neo1p function causes cargo - specific defects in anterograde protein transport early in the secretory pathway and perturbs glycosylation in the Golgi complex.
0.9996	Using conditional alleles of NEO1, we find that loss of ProteinNeo1p function causes cargo - specific defects in anterograde protein transport early in the secretory pathway and perturbs glycosylation in the Golgi complex.

(981)

Rer1 - GFP, a protein that cycles between the ER and Golgi complex in COPI and COPII vesicles, is mislocalized to the vacuole in neo1 - ts at the nonpermissive temperature.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	ProteinRer1 - GFP , a protein that cycles between the ER and Golgi complex in COPI and COPII vesicles, is mislocalized to the vacuole in neo1 - ts at the nonpermissive temperature.
0.9994	Rer1 - GFP, a protein that cycles between the ER and Golgi complex in COPI and COPII vesicles, is mislocalized to the vacuole in Proteinneo1 - ts at the nonpermissive temperature.

(982)

These phenotypes suggest that the anterograde protein transport defect is a secondary consequence of a defect in a COPI - dependent retrograde pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(983)

We propose that loss of lipid asymmetry in the cis Golgi perturbs retrograde protein transport to the ER.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(984)

Fabrication of nickel and chromium nanoparticles using the protein cage of apoferritin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(985)

The iron storage protein, apoferritin, has a cavity in which iron is oxidized and stored as a hydrated oxide core.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(986)

The size of the core is about 7 nm in diameter and is regulated by the cavity size.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(987)

The cavity can be utilized as a nanoreactor to grow inorganic crystals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(988)

We incubated apoferritin in nickel or chromium salt solutions to fabricate hydroxide nanoparticles in the cavity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(989)

By using a solution containing dissolved carbon dioxide and by precisely controlling the pH, we succeeded in fabricating nickel and chromium cores.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(990)

During the hydroxylation process of nickel ions a large portion of the apoferritin precipitated through bulk precipitation of nickel hydroxide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(991)

Bulk precipitation was suppressed by adding ammonium ions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(992)

However, even in the presence of ammonium ions the core did not form using a degassed solution.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(993)

We concluded that carbonate ions were indispensable for core formation and that the ammonium ions prevented precipitation in the bulk solution.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(994)

The optimized condition for nickel core formation was 0. 3 mg / mL horse spleen apoferritin and 5 mM ammonium nickel sulfate in water containing dissolved carbon dioxide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(995)

The pH was maintained at 8. 65 using two buffer solutions : 150 mM HEPES ( pH 7. 5 ) and 195 mM CAPSO ( pH 9. 5 ) with 20 mM ammonium at 23 degrees C.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(996)

The pH had not changed after 48 h.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(997)

After 24 h of incubation, all apoferritins remained in the supernatant and all of them had cores.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(998)

Recombinant L - ferritin showed less precipitation even above a pH of 8. 65.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9852	Recombinant ProteinL - ferritin showed less precipitation even above a pH of 8. 65.

(999)

A chromium core was formed under the following conditions : 0. 1 mg / mL apoferritin, 1 mM ammonium chromium sulfate, 100 mM HEPES ( pH 7. 5 ) with a solution containing dissolved carbon dioxide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1000)

About 80 % of the supernatant apoferritin ( 0. 07 mg / mL ) formed a core.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1001)

In nickel and chromium core formation, carbonate ions would play an important role in accelerating the hydroxylation in the apoferritin cavity compared to the bulk solution outside.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1002)

Histone hyperacetylation in mitosis prevents sister chromatid separation and produces chromosome segregation defects.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9962	ProteinHistone hyperacetylation in mitosis prevents sister chromatid separation and produces chromosome segregation defects.
0.9733	Histone Acetylationhyperacetylation in mitosis prevents sister chromatid separation and produces chromosome segregation defects.

(1003)

Posttranslational modifications of core histones contribute to driving changes in chromatin conformation and compaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9936	Posttranslational modifications of core Proteinhistones contribute to driving changes in chromatin conformation and compaction.

(1004)

Herein, we investigated the role of histone deacetylation on the mitotic process by inhibiting histone deacetylases shortly before mitosis in human primary fibroblasts.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9982	Herein, we investigated the role of histone deacetylation on the mitotic process by inhibiting Proteinhistone deacetylases shortly before mitosis in human primary fibroblasts.
0.9976	Herein, we investigated the role of Proteinhistone deacetylation on the mitotic process by inhibiting histone deacetylases shortly before mitosis in human primary fibroblasts.
0.8260	Herein, we investigated the role of histone Deacetylationdeacetylation on the mitotic process by inhibiting histone deacetylases shortly before mitosis in human primary fibroblasts.

(1005)

Cells entering mitosis with hyperacetylated histones displayed altered chromatin conformation associated with decreased reactivity to the anti - Ser 10 phospho H3 antibody, increased recruitment of protein phosphatase 1 - delta on mitotic chromosomes, and depletion of heterochromatin protein 1 from the centromeric heterochromatin.

Precision: 100.00

Recall: 75.00

F1: 85.71

Score	Text
0.9981	Cells entering mitosis with hyperacetylated Proteinhistones displayed altered chromatin conformation associated with decreased reactivity to the anti - Ser 10 phospho H3 antibody, increased recruitment of protein phosphatase 1 - delta on mitotic chromosomes, and depletion of heterochromatin protein 1 from the centromeric heterochromatin.
0.9821	Cells entering mitosis with Acetylationhyperacetylated histones displayed altered chromatin conformation associated with decreased reactivity to the anti - Ser 10 phospho H3 antibody, increased recruitment of protein phosphatase 1 - delta on mitotic chromosomes, and depletion of heterochromatin protein 1 from the centromeric heterochromatin.
0.9808	Cells entering mitosis with hyperacetylated histones displayed altered chromatin conformation associated with decreased reactivity to the anti - Ser 10 phospho ProteinH3 antibody, increased recruitment of protein phosphatase 1 - delta on mitotic chromosomes, and depletion of heterochromatin protein 1 from the centromeric heterochromatin.
	Cells entering mitosis with hyperacetylated histones displayed altered chromatin conformation associated with decreased reactivity to the anti - Ser 10 phospho H3 antibody, increased recruitment of Proteinprotein phosphatase 1 - delta on mitotic chromosomes, and depletion of heterochromatin protein 1 from the centromeric heterochromatin.

(1006)

Inhibition of histone deacetylation before mitosis produced defective chromosome condensation and impaired mitotic progression in living cells, suggesting that improper chromosome condensation may induce mitotic checkpoint activation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9980	Inhibition of Proteinhistone deacetylation before mitosis produced defective chromosome condensation and impaired mitotic progression in living cells, suggesting that improper chromosome condensation may induce mitotic checkpoint activation.
0.8839	Inhibition of histone Deacetylationdeacetylation before mitosis produced defective chromosome condensation and impaired mitotic progression in living cells, suggesting that improper chromosome condensation may induce mitotic checkpoint activation.

(1007)

In situ hybridization analysis on anaphase cells demonstrated the presence of chromatin bridges, which were caused by persisting cohesion along sister chromatid arms after centromere separation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1008)

Thus, the presence of hyperacetylated chromatin during mitosis impairs proper chromosome condensation during the pre - anaphase stages, resulting in poor sister chromatid resolution.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1009)

Lagging chromosomes consisting of single or paired sisters were also induced by the presence of hyperacetylated histones, indicating that the less constrained centromeric organization associated with heterochromatin protein 1 depletion may promote the attachment of kinetochores to microtubules coming from both poles.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9959	Lagging chromosomes consisting of single or paired sisters were also induced by the presence of hyperacetylated Proteinhistones , indicating that the less constrained centromeric organization associated with heterochromatin protein 1 depletion may promote the attachment of kinetochores to microtubules coming from both poles.
0.9890	Lagging chromosomes consisting of single or paired sisters were also induced by the presence of Acetylationhyperacetylated histones, indicating that the less constrained centromeric organization associated with heterochromatin protein 1 depletion may promote the attachment of kinetochores to microtubules coming from both poles.

(1010)

Hydroxylated kininogens and kinins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9939	Hydroxylated kininogens and Proteinkinins .
0.9793	Hydroxylated Proteinkininogens and kinins.
0.8546	HydroxylationHydroxylated kininogens and kinins.

(1011)

Hydroxyprolyl - 3 - bradykinin was identified in the digest of purified human high molecular weight ( H ) kininogen with plasma kallikrein.

Precision: 66.67

Recall: 66.67

F1: 66.67

Score	Text
0.9824	ProteinHydroxyprolyl - 3 - bradykinin was identified in the digest of purified human high molecular weight ( H ) kininogen with plasma kallikrein.
0.9182	Hydroxyprolyl - 3 - bradykinin was identified in the digest of purified human high molecular weight ( H ) kininogen with Proteinplasma kallikrein .
0.9063	Hydroxyprolyl - 3 - bradykinin was identified in the digest of purified human high molecular weight ( H ) kininogen with plasma Proteinkallikrein .
	Hydroxyprolyl - 3 - bradykinin was identified in the digest of purified human Proteinhigh molecular weight ( H ) kininogen with plasma kallikrein.

(1012)

Hydroxyproline was not detected in the heavy and light chains portions of H kininogen, although they include three possible sites for hydroxylation of proline by proline hydroxylase.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Text
0.9069	Hydroxyproline was not detected in the heavy and light chains portions of ProteinH kininogen , although they include three possible sites for hydroxylation of proline by proline hydroxylase.
0.7060	Hydroxyproline was not detected in the heavy and light chains portions of H kininogen, although they include three possible sites for Hydroxylationhydroxylation of proline by proline hydroxylase.
0.5596	Hydroxyproline was not detected in the heavy and light chains portions of H Proteinkininogen , although they include three possible sites for hydroxylation of proline by proline hydroxylase.

(1013)

The content of hydroxyprolyl - 3 - bradykinin in H kininogen from individual plasmas varied from 14 % to 64 % of total kinin.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9973	The content of hydroxyprolyl - 3 - bradykinin in H kininogen from individual plasmas varied from 14 % to 64 % of total Proteinkinin .
0.9842	The content of Proteinhydroxyprolyl - 3 - bradykinin in H kininogen from individual plasmas varied from 14 % to 64 % of total kinin.
0.8891	The content of hydroxyprolyl - 3 - bradykinin in ProteinH kininogen from individual plasmas varied from 14 % to 64 % of total kinin.
0.5702	The content of hydroxyprolyl - 3 - bradykinin in H Proteinkininogen from individual plasmas varied from 14 % to 64 % of total kinin.

(1014)

The present results and our previous results indicate that only kinin moity in H kininogen from human and monkey plasmas has been partially hydroxylated post - translationally by proline - 4 - hydroxylase.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9690	The present results and our previous results indicate that only Proteinkinin moity in H kininogen from human and monkey plasmas has been partially hydroxylated post - translationally by proline - 4 - hydroxylase.
0.8933	The present results and our previous results indicate that only kinin moity in ProteinH kininogen from human and monkey plasmas has been partially hydroxylated post - translationally by proline - 4 - hydroxylase.
0.6976	The present results and our previous results indicate that only kinin moity in H kininogen from human and monkey plasmas has been partially Hydroxylationhydroxylated post - translationally by proline - 4 - hydroxylase.
0.6174	The present results and our previous results indicate that only kinin moity in H kininogen from human and monkey plasmas has been partially hydroxylated post - translationally by Proteinproline - 4 - hydroxylase .
0.5876	The present results and our previous results indicate that only kinin moity in H Proteinkininogen from human and monkey plasmas has been partially hydroxylated post - translationally by proline - 4 - hydroxylase.
0.5086	The present results and our previous results indicate that only kinin moity in ProteinH kininogen from human and monkey plasmas has been partially hydroxylated post - translationally by proline - 4 - hydroxylase.

(1015)

Purification and characterization of soluble forms of human and rat stem cell factor recombinantly expressed by Escherichia coli and by Chinese hamster ovary cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9407	Purification and characterization of soluble forms of human and rat Proteinstem cell factor recombinantly expressed by Escherichia coli and by Chinese hamster ovary cells.

(1016)

Stem cell factor ( SCF ) is a novel, early - acting hematopoietic factor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9647	Stem cell factor Protein( SCF ) is a novel, early - acting hematopoietic factor.
0.6746	ProteinStem cell factor ( SCF ) is a novel, early - acting hematopoietic factor.

(1017)

It was isolated from the medium of a rat cell line in a soluble, processed form ( Zsebo et al., 1990, Cell 63, 195 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1018)

The cloned human and rat genes encode the soluble form plus additional C - terminal amino acids including a hydrophobic transmembrane domain ( Martin et al., 1990, Cell 63, 203 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1019)

We have recombinantly expressed forms of human and rat SCF corresponding to the soluble, processed form in Escherichia coli and in Chinese hamster ovary ( CHO ) cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	We have recombinantly expressed forms of human and rat ProteinSCF corresponding to the soluble, processed form in Escherichia coli and in Chinese hamster ovary ( CHO ) cells.

(1020)

After expression in E. coli, folding and oxidation of the SCF polypeptides are required.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9970	After expression in E. coli, folding and oxidation of the ProteinSCF polypeptides are required.

(1021)

The SCFs expressed in CHO cells are secreted into the medium in active state and, like the natural SCF, are glycosylated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9934	The ProteinSCFs expressed in CHO cells are secreted into the medium in active state and, like the natural SCF, are glycosylated.
0.9844	The SCFs expressed in CHO cells are secreted into the medium in active state and, like the natural ProteinSCF , are glycosylated.
0.9834	The SCFs expressed in CHO cells are secreted into the medium in active state and, like the natural SCF, are Glycosylationglycosylated .

(1022)

Purification of the recombinant SCFs is described.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	Purification of the recombinant ProteinSCFs is described.

(1023)

Biological and biochemical characterization includes activity toward responsive human and mouse cell lines, N - terminal amino acid sequences, disulfide bond linkages, and sites of glycosylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1024)

Inhibition of fusion by neutralizing monoclonal antibodies to the haemagglutinin - neuraminidase glycoprotein of Newcastle disease virus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9809	Inhibition of fusion by neutralizing monoclonal antibodies to the Proteinhaemagglutinin - neuraminidase glycoprotein of Newcastle disease virus.

(1025)

The majority of neutralizing monoclonal antibodies ( MAbs ) to the haemagglutinin - neuraminidase ( HN ) glycoprotein of Newcastle disease virus prevent attachment of the virus to cellular receptors and inhibits virion - induced fusion from without ( FFWO ) and fusion from within ( FFWI ) mediated by the virus glycoprotein - laden infected cell surface.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9728	The majority of neutralizing monoclonal antibodies ( MAbs ) to the Proteinhaemagglutinin - neuraminidase ( HN ) glycoprotein of Newcastle disease virus prevent attachment of the virus to cellular receptors and inhibits virion - induced fusion from without ( FFWO ) and fusion from within ( FFWI ) mediated by the virus glycoprotein - laden infected cell surface.
0.9529	The majority of neutralizing monoclonal antibodies ( MAbs ) to the haemagglutinin - neuraminidase Protein( HN ) glycoprotein of Newcastle disease virus prevent attachment of the virus to cellular receptors and inhibits virion - induced fusion from without ( FFWO ) and fusion from within ( FFWI ) mediated by the virus glycoprotein - laden infected cell surface.

(1026)

For these antibodies, the inhibition of fusion is presumed to be the result of the prevention of HN - mediated bridging of potential fusion partners.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9948	For these antibodies, the inhibition of fusion is presumed to be the result of the prevention of ProteinHN - mediated bridging of potential fusion partners.

(1027)

MAbs against antigenic sites 3 and 4 neutralize virus infectivity, but by a mechanism other than the prevention of attachment, the exact nature of which remains to be established.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1028)

Antibodies to both of these sites effectively inhibit virion - induced FFWO, even when the inducing virus is not infectious.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1029)

This is consistent with the mechanism of neutralization of these MAbs involving the inhibition of an early, post - attachment step in infection.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1030)

MAbs to site 3 also inhibit FFWI, but those to site 4 do not, even when added at high concentrations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1031)

This suggests that the requirement for HN may be different in the two modes of fusion.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9972	This suggests that the requirement for ProteinHN may be different in the two modes of fusion.

(1032)

The epitopes recognized by MAbs to sites 3 and 4 have been delineated by the identification of individual nucleotide substitutions in the HN genes of neutralization escape variants.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9983	The epitopes recognized by MAbs to sites 3 and 4 have been delineated by the identification of individual nucleotide substitutions in the ProteinHN genes of neutralization escape variants.

(1033)

Some of the deduced amino acid substitutions result in additional N - linked glycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.

Precision: 42.86

Recall: 100.00

F1: 60.00

Score	Text
0.9536	Some of the deduced amino acid substitutions result in additional N - linked glycosylation sites in ProteinHN , which are utilized and presumably account for the escape from neutralization.
0.8201	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.8191	Some of the deduced amino acid substitutions result in additional N - linked glycosylation sites in HN, which are Glycosylationutilized and presumably account for the escape from neutralization.
0.8283	Some of the deduced amino acid substitutions result in additional N - linked Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.7204	Some of the deduced amino acid substitutions result in additional N - linked Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.6617	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.4769	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.

(1034)

The influence of N - linked glycosylation on the antigenicity and immunogenicity of rubella virus E1 glycoprotein.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9976	The influence of N - linked glycosylation on the antigenicity and immunogenicity of rubella virus ProteinE1 glycoprotein.
0.8220	The influence of GlycosylationN - linked glycosylation on the antigenicity and immunogenicity of rubella virus E1 glycoprotein.
0.8842	The influence of N - linked Glycosylationglycosylation on the antigenicity and immunogenicity of rubella virus E1 glycoprotein.

(1035)

Rubella virus E1 glycoprotein contains three functional N - linked glycosylation sites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	Rubella virus ProteinE1 glycoprotein contains three functional N - linked glycosylation sites.

(1036)

The role of N - linked glycosylation on the antigenicity and immunogenicity of E1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Text
0.9989	The role of N - linked glycosylation on the antigenicity and immunogenicity of ProteinE1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.
0.9953	The role of N - linked glycosylation on the antigenicity and immunogenicity of E1 glycoprotein was studied using vaccinia recombinants expressing ProteinE1 glycosylation mutants.
0.8824	The role of GlycosylationN - linked glycosylation on the antigenicity and immunogenicity of E1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.
0.8918	The role of N - linked Glycosylationglycosylation on the antigenicity and immunogenicity of E1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.
0.7445	The role of GlycosylationN - linked glycosylation on the antigenicity and immunogenicity of E1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.

(1037)

Expressed E1 glycosylation mutant proteins were recognized by a panel of E1 - specific monoclonal antibodies in radioimmunoprecipitation, immunofluorescence, and immunoblotting, indicating that carbohydrate side chains on E1 are not involved in the constitution of epitopes recognized by these monoclonal antibodies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9990	Expressed E1 glycosylation mutant proteins were recognized by a panel of E1 - specific monoclonal antibodies in radioimmunoprecipitation, immunofluorescence, and immunoblotting, indicating that carbohydrate side chains on ProteinE1 are not involved in the constitution of epitopes recognized by these monoclonal antibodies.
0.9989	Expressed E1 glycosylation mutant proteins were recognized by a panel of ProteinE1 - specific monoclonal antibodies in radioimmunoprecipitation, immunofluorescence, and immunoblotting, indicating that carbohydrate side chains on E1 are not involved in the constitution of epitopes recognized by these monoclonal antibodies.
0.9976	Expressed ProteinE1 glycosylation mutant proteins were recognized by a panel of E1 - specific monoclonal antibodies in radioimmunoprecipitation, immunofluorescence, and immunoblotting, indicating that carbohydrate side chains on E1 are not involved in the constitution of epitopes recognized by these monoclonal antibodies.

(1038)

This observation was further supported by the fact that removal of oligosaccharides on E1 by glycosidase digestion did not significantly change the antigenicity of E1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	This observation was further supported by the fact that removal of oligosaccharides on E1 by glycosidase digestion did not significantly change the antigenicity of ProteinE1 .
0.9968	This observation was further supported by the fact that removal of oligosaccharides on ProteinE1 by glycosidase digestion did not significantly change the antigenicity of E1.

(1039)

All the glycosylation mutants were capable of eliciting anti - RV E1 antibodies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9974	All the glycosylation mutants were capable of eliciting anti - RV ProteinE1 antibodies.

(1040)

The single glycosylation mutants ( G1, G2, and G3 ), but not the double mutant ( G23 ) or the triple mutant ( G123 ), were found to be capable of inducing virus neutralizing antibodies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1041)

Among the single glycosylation mutants, only G2 and G3 were active in producing hemagglutination inhibition antibodies in mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1042)

Our findings suggest that although carbohydrate on E1 is not directly involved in the antigenic structures of E1, it is important in maintaining proper protein folding and stable conformation for expression of immunological epitopes on E1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9982	Our findings suggest that although carbohydrate on E1 is not directly involved in the antigenic structures of ProteinE1 , it is important in maintaining proper protein folding and stable conformation for expression of immunological epitopes on E1.
0.9982	Our findings suggest that although carbohydrate on ProteinE1 is not directly involved in the antigenic structures of E1, it is important in maintaining proper protein folding and stable conformation for expression of immunological epitopes on E1.
0.9979	Our findings suggest that although carbohydrate on E1 is not directly involved in the antigenic structures of E1, it is important in maintaining proper protein folding and stable conformation for expression of immunological epitopes on ProteinE1 .

(1043)

Glycosylation of the interleukin - 1 receptor type I is required for optimal binding of interleukin - 1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9534	GlycosylationGlycosylation of the interleukin - 1 receptor type I is required for optimal binding of interleukin - 1.
0.8109	Glycosylation of the Proteininterleukin - 1 receptor type I is required for optimal binding of interleukin - 1.

(1044)

The two types of cell surface receptors for interleukin - 1 ( IL - 1 ) are glycoproteins that contain N - glycosidic chains on their extracellular portions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1045)

To determine the role of glycosylation of the IL - 1 receptor type I ( IL - 1RtI ) in the binding and function of IL - 1, we used four plant lectins and glycosidase treatment on two different T - cell lines ( EL4 - 6. 1 and D10S ) and expressing high number of binding sites for IL - 1.

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Text
0.9895	To determine the role of Glycosylationglycosylation of the IL - 1 receptor type I ( IL - 1RtI ) in the binding and function of IL - 1, we used four plant lectins and glycosidase treatment on two different T - cell lines ( EL4 - 6. 1 and D10S ) and expressing high number of binding sites for IL - 1.
0.9695	To determine the role of glycosylation of the IL - 1 receptor type I Protein( IL - 1RtI ) in the binding and function of IL - 1, we used four plant lectins and glycosidase treatment on two different T - cell lines ( EL4 - 6. 1 and D10S ) and expressing high number of binding sites for IL - 1.
0.9090	To determine the role of glycosylation of the ProteinIL - 1 receptor type I ( IL - 1RtI ) in the binding and function of IL - 1, we used four plant lectins and glycosidase treatment on two different T - cell lines ( EL4 - 6. 1 and D10S ) and expressing high number of binding sites for IL - 1.
0.8954	To determine the role of glycosylation of the IL - 1 receptor type I ( IL - 1RtI ) in the binding and function of IL - 1, we used four plant lectins and glycosidase treatment on two different T - cell lines ( EL4 - 6. 1 and D10S ) and expressing high number of binding sites for ProteinIL - 1 .
0.6039	To determine the role of glycosylation of the IL - 1 receptor type I ( IL - 1RtI ) in the binding and function of ProteinIL - 1 , we used four plant lectins and glycosidase treatment on two different T - cell lines ( EL4 - 6. 1 and D10S ) and expressing high number of binding sites for IL - 1.

(1046)

The lectins wheat germ agglutinin, phytohemagglutinin, and concanavalin A inhibited in a dose - response manner the IL - 1 - induced proliferation of D10S cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9197	The lectins wheat germ agglutinin, phytohemagglutinin, and Proteinconcanavalin A inhibited in a dose - response manner the IL - 1 - induced proliferation of D10S cells.

(1047)

Binding of IL - 1 was blocked and radioactive IL - 1 was displaced from these cells by these lectins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1048)

Specific sugars ( GlcNAc, NeuAc, Gal - GlcNAc - Man, Man, or alpha - MeMan ) did not themselves affect IL - 1 binding but reversed the blocking effects of the lectins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1049)

The two cell lines differed in their responses to the lectin - mediated inhibition of IL - 1 binding.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1050)

Digestion by N - glycosidase significantly decreased the capacity of cells to bind IL - 1, and reduced by approximately 20, 000 D the M ( r ) of the IL - 1RtI.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9902	Digestion by N - glycosidase significantly decreased the capacity of cells to bind IL - 1, and reduced by approximately 20, 000 D the M ( r ) of the ProteinIL - 1RtI .

(1051)

Neuraminidase and O - glycanase treatment did not alter the binding of IL - 1 to D10S or EL4 - 6. 1 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1052)

This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this N - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.

Precision: 55.56

Recall: 100.00

F1: 71.43

Score	Text
0.9839	This study demonstrates that Glycosylationglycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this N - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.9773	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of Glycosylationglycosylation can vary in cells of different lineage, and that this N - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.9554	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this GlycosylationN - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.9376	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked Entitycarbohydrates , that the degree of glycosylation can vary in cells of different lineage, and that this N - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.7725	This study demonstrates that glycosylation of the extracellular domain of the ProteinIL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this N - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.9880	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this N - linked glycosylation appears to be essential for optimal binding and activity of ProteinIL - 1 to its type I receptor.
0.9640	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this N - linked Glycosylationglycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.8293	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this GlycosylationN - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.4637	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to GlycosylationN - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this N - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.

(1053)

Expression of Cpgp40 / 15 in Toxoplasma gondii : a surrogate system for the study of Cryptosporidium glycoprotein antigens.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	Expression of ProteinCpgp40 / 15 in Toxoplasma gondii : a surrogate system for the study of Cryptosporidium glycoprotein antigens.

(1054)

Cryptosporidium parvum is a waterborne enteric coccidian that causes diarrheal disease in a wide range of hosts.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1055)

Development of successful therapies is hampered by the inability to culture the parasite and the lack of a transfection system for genetic manipulation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1056)

The glycoprotein products of the Cpgp40 / 15 gene, gp40 and gp15, are involved in C. parvum sporozoite attachment to and invasion of host cells and, as such, may be good targets for anticryptosporidial therapies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	The glycoprotein products of the Cpgp40 / 15 gene, gp40 and Proteingp15 , are involved in C. parvum sporozoite attachment to and invasion of host cells and, as such, may be good targets for anticryptosporidial therapies.
0.9996	The glycoprotein products of the Cpgp40 / 15 gene, Proteingp40 and gp15, are involved in C. parvum sporozoite attachment to and invasion of host cells and, as such, may be good targets for anticryptosporidial therapies.
0.9990	The glycoprotein products of the ProteinCpgp40 / 15 gene, gp40 and gp15, are involved in C. parvum sporozoite attachment to and invasion of host cells and, as such, may be good targets for anticryptosporidial therapies.

(1057)

However, the function of these antigens appears to be dependent on the presence of multiple O - linked alpha - N - acetylgalactosamine ( alpha - GalNAc ) determinants.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9575	However, the function of these antigens appears to be dependent on the presence of multiple O - linked Entityalpha - N - acetylgalactosamine ( alpha - GalNAc ) determinants.
0.9502	However, the function of these antigens appears to be dependent on the presence of multiple GlycosylationO - linked alpha - N - acetylgalactosamine ( alpha - GalNAc ) determinants.
0.9233	However, the function of these antigens appears to be dependent on the presence of multiple O - linked alpha - N - acetylgalactosamine Entity( alpha - GalNAc ) determinants.
0.6846	However, the function of these antigens appears to be dependent on the presence of multiple O - linked Entityalpha - N - acetylgalactosamine ( alpha - GalNAc ) determinants.

(1058)

A eukaryotic expression system that would produce proteins bearing glycosylation patterns similar to those found on the native C. parvum glycoproteins would greatly facilitate the molecular and functional characterization of these antigens.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1059)

As a unique approach to this problem, the Cpgp40 / 15 gene was transiently expressed in Toxoplasma gondii, and the expressed recombinant glycoproteins were characterized.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	As a unique approach to this problem, the ProteinCpgp40 / 15 gene was transiently expressed in Toxoplasma gondii, and the expressed recombinant glycoproteins were characterized.

(1060)

Antisera to gp40 and gp15 reacted with the surface membranes of tachyzoites expressing the Cpgp40 / 15 construct, and this reactivity colocalized with that of antiserum to the T. gondii surface protein SAG1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Antisera to gp40 and Proteingp15 reacted with the surface membranes of tachyzoites expressing the Cpgp40 / 15 construct, and this reactivity colocalized with that of antiserum to the T. gondii surface protein SAG1.
0.9996	Antisera to gp40 and gp15 reacted with the surface membranes of tachyzoites expressing the ProteinCpgp40 / 15 construct, and this reactivity colocalized with that of antiserum to the T. gondii surface protein SAG1.
0.9996	Antisera to Proteingp40 and gp15 reacted with the surface membranes of tachyzoites expressing the Cpgp40 / 15 construct, and this reactivity colocalized with that of antiserum to the T. gondii surface protein SAG1.
0.9995	Antisera to gp40 and gp15 reacted with the surface membranes of tachyzoites expressing the Cpgp40 / 15 construct, and this reactivity colocalized with that of antiserum to the T. gondii surface protein ProteinSAG1 .

(1061)

Surface membrane localization was dependent on the presence of the glycophosphatidylinositol anchor attachment site present in the gp15 coding sequence.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9981	Surface membrane localization was dependent on the presence of the glycophosphatidylinositol anchor attachment site present in the Proteingp15 coding sequence.

(1062)

The presence of terminal O - linked alpha - GalNAc determinants on the T. gondii recombinant gp40 was confirmed by reactivity with Helix pomatia lectin and the monoclonal antibody 4E9, which recognizes alpha - GalNAc residues, and digestion with alpha - N - acetylgalactosaminidase.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9955	The presence of terminal O - linked alpha - GalNAc determinants on the T. gondii recombinant Proteingp40 was confirmed by reactivity with Helix pomatia lectin and the monoclonal antibody 4E9, which recognizes alpha - GalNAc residues, and digestion with alpha - N - acetylgalactosaminidase.
0.9929	The presence of terminal O - linked Entityalpha - GalNAc determinants on the T. gondii recombinant gp40 was confirmed by reactivity with Helix pomatia lectin and the monoclonal antibody 4E9, which recognizes alpha - GalNAc residues, and digestion with alpha - N - acetylgalactosaminidase.
0.9624	The presence of terminal GlycosylationO - linked alpha - GalNAc determinants on the T. gondii recombinant gp40 was confirmed by reactivity with Helix pomatia lectin and the monoclonal antibody 4E9, which recognizes alpha - GalNAc residues, and digestion with alpha - N - acetylgalactosaminidase.
0.9794	The presence of terminal O - linked alpha - GalNAc determinants on the T. gondii recombinant gp40 was confirmed by reactivity with Helix pomatia lectin and the monoclonal antibody 4E9, which recognizes alpha - GalNAc residues, and digestion with Proteinalpha - N - acetylgalactosaminidase .
0.7244	The presence of terminal O - linked alpha - GalNAc determinants on the T. gondii recombinant gp40 was confirmed by reactivity with Helix pomatia lectin and the monoclonal antibody 4E9, which recognizes Entityalpha - GalNAc residues, and digestion with alpha - N - acetylgalactosaminidase.
0.5534	The presence of terminal O - linked alpha - GalNAc determinants on the T. gondii recombinant gp40 was confirmed by reactivity with Helix pomatia lectin and the monoclonal antibody 4E9, which recognizes Entityalpha - GalNAc residues , and digestion with alpha - N - acetylgalactosaminidase.

(1063)

In addition to appropriate localization and glycosylation, T. gondii apparently processes the gp40 / 15 precursor into the gp40 and gp15 component glycopolypeptides, albeit inefficiently.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	In addition to appropriate localization and glycosylation, T. gondii apparently processes the gp40 / 15 precursor into the gp40 and Proteingp15 component glycopolypeptides, albeit inefficiently.
0.9990	In addition to appropriate localization and glycosylation, T. gondii apparently processes the gp40 / 15 precursor into the Proteingp40 and gp15 component glycopolypeptides, albeit inefficiently.
0.9982	In addition to appropriate localization and glycosylation, T. gondii apparently processes the Proteingp40 / 15 precursor into the gp40 and gp15 component glycopolypeptides, albeit inefficiently.

(1064)

These results suggest that a surrogate system using T. gondii for the study of Cryptosporidium biology may be useful.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1065)

Aberrant methylation of DAP - kinase in therapy - related acute myeloid leukemia and myelodysplastic syndromes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9962	Aberrant methylation of ProteinDAP - kinase in therapy - related acute myeloid leukemia and myelodysplastic syndromes.
0.9670	Aberrant DNA_methylationmethylation of DAP - kinase in therapy - related acute myeloid leukemia and myelodysplastic syndromes.

(1066)

Death - associated protein kinase ( DAP - kinase ), a proapoptotic serine / threonine kinase, is a candidate tumor suppressor gene.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9698	Death - associated protein kinase Protein( DAP - kinase ) , a proapoptotic serine / threonine kinase, is a candidate tumor suppressor gene.
0.8802	ProteinDeath - associated protein kinase ( DAP - kinase ), a proapoptotic serine / threonine kinase, is a candidate tumor suppressor gene.

(1067)

We studied the methylation status of DAP - kinase of 194 bone marrow samples from 160 patients with acute myeloid leukemia ( AML ) and 34 with a myelodysplastic syndrome ( MDS ) at the time of initial diagnosis by polymerase chain reaction ( PCR ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9948	We studied the methylation status of ProteinDAP - kinase of 194 bone marrow samples from 160 patients with acute myeloid leukemia ( AML ) and 34 with a myelodysplastic syndrome ( MDS ) at the time of initial diagnosis by polymerase chain reaction ( PCR ).
0.9888	We studied the DNA_methylationmethylation status of DAP - kinase of 194 bone marrow samples from 160 patients with acute myeloid leukemia ( AML ) and 34 with a myelodysplastic syndrome ( MDS ) at the time of initial diagnosis by polymerase chain reaction ( PCR ).

(1068)

Hypermethylation of DAP - kinase was present in 27. 5 % ( 44 of 160 ) of AML and in 47 % ( 16 of 34 ) of MDS specimens and significantly correlated to loss of DAP - kinase expression ( P =. 008 ).

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9984	Hypermethylation of ProteinDAP - kinase was present in 27. 5 % ( 44 of 160 ) of AML and in 47 % ( 16 of 34 ) of MDS specimens and significantly correlated to loss of DAP - kinase expression ( P =. 008 ).
0.9769	DNA_methylationHypermethylation of DAP - kinase was present in 27. 5 % ( 44 of 160 ) of AML and in 47 % ( 16 of 34 ) of MDS specimens and significantly correlated to loss of DAP - kinase expression ( P =. 008 ).
0.8817	Hypermethylation of DAP - kinase was present in 27. 5 % ( 44 of 160 ) of AML and in 47 % ( 16 of 34 ) of MDS specimens and significantly correlated to loss of ProteinDAP - kinase expression ( P =. 008 ).

(1069)

It was significantly more frequent in AML secondary to therapy for other malignancies ( s - AML ; 14 of 29, 48. 3 % ), as compared to de novo AML ( 30 of 131, 22. 9 %, P =. 01 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1070)

DAP - kinase hypermethylation in AML was associated with myelodysplastic changes in the bone marrow at the time of the initial diagnosis ( P =. 002 ) and with the presence of cytogenetic abnormalities ( P =. 02 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9871	ProteinDAP - kinase hypermethylation in AML was associated with myelodysplastic changes in the bone marrow at the time of the initial diagnosis ( P =. 002 ) and with the presence of cytogenetic abnormalities ( P =. 02 ).
0.9825	DAP - kinase DNA_methylationhypermethylation in AML was associated with myelodysplastic changes in the bone marrow at the time of the initial diagnosis ( P =. 002 ) and with the presence of cytogenetic abnormalities ( P =. 02 ).

(1071)

Alteration in the apoptotic response due to the loss of DAP - kinase function may be an early event in the transformation pathway to secondary leukemia via myelodysplasia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	Alteration in the apoptotic response due to the loss of ProteinDAP - kinase function may be an early event in the transformation pathway to secondary leukemia via myelodysplasia.

(1072)

Molecular pathology of atypical polypoid adenomyoma of the uterus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1073)

Atypical polypoid adenomyoma ( APA ) is an uncommon and benign tumor of the uterus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1074)

In some patients, however, APA has been found to coexist with or to precede the development of an endometrioid adenocarcinoma similarly to complex endometrial hyperplasia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1075)

The molecular changes underlying the progression from APA to adenocarcinoma are unknown.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1076)

DNA from paraffin - embedded tissue of 6 APAs was evaluated for microsatellite instability ( MI ), MLH - 1 promoter hypermethylation, and CTNNB - 1 mutations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9979	DNA from paraffin - embedded tissue of 6 APAs was evaluated for microsatellite instability ( MI ), MLH - 1 promoter hypermethylation, and ProteinCTNNB - 1 mutations.
0.9890	DNA from paraffin - embedded tissue of 6 APAs was evaluated for microsatellite instability ( MI ), MLH - 1 Entitypromoter hypermethylation, and CTNNB - 1 mutations.
0.9818	DNA from paraffin - embedded tissue of 6 APAs was evaluated for microsatellite instability ( MI ), MLH - 1 promoter DNA_methylationhypermethylation , and CTNNB - 1 mutations.
0.9682	DNA from paraffin - embedded tissue of 6 APAs was evaluated for microsatellite instability ( MI ), ProteinMLH - 1 promoter hypermethylation, and CTNNB - 1 mutations.

(1077)

Tissue sections were also subjected to MLH - 1, MSH - 2, and beta - catenin immunostaining.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	Tissue sections were also subjected to MLH - 1, ProteinMSH - 2 , and beta - catenin immunostaining.
0.9981	Tissue sections were also subjected to MLH - 1, MSH - 2, and Proteinbeta - catenin immunostaining.
0.9925	Tissue sections were also subjected to ProteinMLH - 1 , MSH - 2, and beta - catenin immunostaining.

(1078)

MI was not detected in any case.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1079)

Two tumors exhibited MLH - 1 promoter hypermethylation and showed focal negative MHL - 1 immunostaining ; 1 of these showed marked architectural complexity and cellular pleomorphism.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9870	Two tumors exhibited ProteinMLH - 1 promoter hypermethylation and showed focal negative MHL - 1 immunostaining ; 1 of these showed marked architectural complexity and cellular pleomorphism.
0.9864	Two tumors exhibited MLH - 1 promoter DNA_methylationhypermethylation and showed focal negative MHL - 1 immunostaining ; 1 of these showed marked architectural complexity and cellular pleomorphism.
0.9824	Two tumors exhibited MLH - 1 Entitypromoter hypermethylation and showed focal negative MHL - 1 immunostaining ; 1 of these showed marked architectural complexity and cellular pleomorphism.

(1080)

Five cases presented beta - catenin nuclear immunoreactivity, but none of them had CTNNB - 1 mutations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Five cases presented Proteinbeta - catenin nuclear immunoreactivity, but none of them had CTNNB - 1 mutations.
0.9971	Five cases presented beta - catenin nuclear immunoreactivity, but none of them had ProteinCTNNB - 1 mutations.

(1081)

The results of this study suggest that APA and complex endometrial hyperplasia may share some molecular alterations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1082)

Some APAs exhibit MLH - 1 promoter hypermethylation with focal lack of MLH - 1 immunostaining, a molecular abnormality involved in the transition from complex atypical hyperplasia to endometrioid adenocarcinoma.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9910	Some APAs exhibit MLH - 1 promoter DNA_methylationhypermethylation with focal lack of MLH - 1 immunostaining, a molecular abnormality involved in the transition from complex atypical hyperplasia to endometrioid adenocarcinoma.
0.9863	Some APAs exhibit ProteinMLH - 1 promoter hypermethylation with focal lack of MLH - 1 immunostaining, a molecular abnormality involved in the transition from complex atypical hyperplasia to endometrioid adenocarcinoma.
0.9695	Some APAs exhibit MLH - 1 Entitypromoter hypermethylation with focal lack of MLH - 1 immunostaining, a molecular abnormality involved in the transition from complex atypical hyperplasia to endometrioid adenocarcinoma.
0.8882	Some APAs exhibit MLH - 1 promoter hypermethylation with focal lack of ProteinMLH - 1 immunostaining, a molecular abnormality involved in the transition from complex atypical hyperplasia to endometrioid adenocarcinoma.

(1083)

A common polymorphism in the oxygen - dependent degradation ( ODD ) domain of hypoxia inducible factor - 1alpha ( HIF - 1alpha ) does not impair Pro - 564 hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9941	A common polymorphism in the oxygen - dependent degradation ( ODD ) domain of hypoxia inducible factor - 1alpha ( HIF - 1alpha ) does not impair EntityPro - 564 hydroxylation.
0.9544	A common polymorphism in the oxygen - dependent degradation ( ODD ) domain of hypoxia inducible factor - 1alpha ( HIF - 1alpha ) does not impair Pro - 564 Hydroxylationhydroxylation .
0.9011	A common polymorphism in the oxygen - dependent degradation ( ODD ) domain of hypoxia inducible factor - 1alpha Protein( HIF - 1alpha ) does not impair Pro - 564 hydroxylation.
0.8000	A common polymorphism in the oxygen - dependent degradation ( ODD ) domain of Proteinhypoxia inducible factor - 1alpha ( HIF - 1alpha ) does not impair Pro - 564 hydroxylation.

(1084)

BACKGROUND : The hypoxia - inducible factor ( HIF ) transcription complex, which is activated by low oxygen tension, controls a diverse range of cellular processes including angiogenesis and erythropoiesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1085)

Under normoxic conditions, the alpha subunit of HIF is rapidly degraded in a manner dependent on hydroxylation of two conserved proline residues at positions 402 and 564 in HIF - 1alpha in the oxygen - dependent degradation ( ODD ) domain.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9968	Under normoxic conditions, the alpha subunit of HIF is rapidly degraded in a manner dependent on hydroxylation of two conserved proline residues at positions 402 and 564 in ProteinHIF - 1alpha in the oxygen - dependent degradation ( ODD ) domain.
0.9745	Under normoxic conditions, the alpha subunit of HIF is rapidly degraded in a manner dependent on Hydroxylationhydroxylation of two conserved proline residues at positions 402 and 564 in HIF - 1alpha in the oxygen - dependent degradation ( ODD ) domain.
0.8789	Under normoxic conditions, the alpha subunit of HIF is rapidly degraded in a manner dependent on hydroxylation of two conserved Entityproline residues at positions 402 and 564 in HIF - 1alpha in the oxygen - dependent degradation ( ODD ) domain.
0.6517	Under normoxic conditions, the alpha subunit of HIF is rapidly degraded in a manner dependent on hydroxylation of two conserved Entityproline residues at positions 402 and 564 in HIF - 1alpha in the oxygen - dependent degradation ( ODD ) domain.

(1086)

This allows subsequent recognition by the von Hippel - Lindau ( VHL ) tumor suppressor protein, which targets HIF for degradation by the ubiquitin - proteasome pathway.

Precision: 50.00

Recall: 50.00

F1: 50.00

Score	Text
0.9993	This allows subsequent recognition by the von Hippel - Lindau ( VHL ) tumor suppressor protein, which targets HIF for degradation by the Proteinubiquitin - proteasome pathway.
0.8779	This allows subsequent recognition by the von Hippel - Lindau Protein( VHL ) tumor suppressor protein, which targets HIF for degradation by the ubiquitin - proteasome pathway.
	This allows subsequent recognition by the Proteinvon Hippel - Lindau ( VHL ) tumor suppressor protein, which targets HIF for degradation by the ubiquitin - proteasome pathway.

(1087)

Under hypoxic conditions, prolyl hydroxylation of HIF is inhibited, allowing it to escape VHL - mediated degradation.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.9610	Under hypoxic conditions, prolyl hydroxylation of HIF is inhibited, allowing it to escape ProteinVHL - mediated degradation.

(1088)

The transcriptional regulation of the erythropoietin gene by HIF raises the possibility that HIF may play a role in disorders of erythropoiesis, such as idiopathic erythrocytosis ( IE ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9959	The transcriptional regulation of the Proteinerythropoietin gene by HIF raises the possibility that HIF may play a role in disorders of erythropoiesis, such as idiopathic erythrocytosis ( IE ).

(1089)

RESULTS : Patients with IE were screened for changes in the HIF - 1alpha coding sequence, and a change in the ODD domain that converts Pro - 582 to Ser was identified in several patients.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	RESULTS : Patients with IE were screened for changes in the ProteinHIF - 1alpha coding sequence, and a change in the ODD domain that converts Pro - 582 to Ser was identified in several patients.

(1090)

This same change, however, was also detected at a significant frequency, 0. 073, in unaffected controls compared to 0. 109 in the IE patient group.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1091)

In vitro hydroxylation assays examining this amino acid change failed to reveal a discernible effect on HIF hydroxylation at Pro - 564.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1092)

CONCLUSION : The Pro582Ser change represents a common polymorphism of HIF - 1alpha that does not impair HIF - 1alpha prolyl hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9946	CONCLUSION : The Pro582Ser change represents a common polymorphism of ProteinHIF - 1alpha that does not impair HIF - 1alpha prolyl hydroxylation.
0.9924	CONCLUSION : The Pro582Ser change represents a common polymorphism of HIF - 1alpha that does not impair ProteinHIF - 1alpha prolyl hydroxylation.
0.9489	CONCLUSION : The Pro582Ser change represents a common polymorphism of HIF - 1alpha that does not impair HIF - 1alpha prolyl Hydroxylationhydroxylation .
0.8726	CONCLUSION : The Pro582Ser change represents a common polymorphism of HIF - 1alpha that does not impair HIF - 1alpha Entityprolyl hydroxylation.

(1093)

Although the Pro582Ser polymorphism is located in the ODD domain of HIF - 1alpha it does not diminish the association of HIF - 1alpha with VHL.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	Although the Pro582Ser polymorphism is located in the ODD domain of HIF - 1alpha it does not diminish the association of HIF - 1alpha with ProteinVHL .
0.9993	Although the Pro582Ser polymorphism is located in the ODD domain of HIF - 1alpha it does not diminish the association of ProteinHIF - 1alpha with VHL.
0.9988	Although the Pro582Ser polymorphism is located in the ODD domain of ProteinHIF - 1alpha it does not diminish the association of HIF - 1alpha with VHL.

(1094)

Thus, it is unlikely that this polymorphism accounts for the erythrocytosis in the group of IE patients studied.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1095)

Expression of histone acetyltransferases was down - regulated in poly ( ADP - ribose ) polymerase - 1 - deficient murine cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9959	Expression of Proteinhistone acetyltransferases was down - regulated in poly ( ADP - ribose ) polymerase - 1 - deficient murine cells.
0.9189	Expression of histone acetyltransferases was down - regulated in Proteinpoly ( ADP - ribose ) polymerase - 1 - deficient murine cells.

(1096)

NF - kappaB - dependent, as well as human immunodeficiency virus type - 1 ( HIV - 1 ) long terminal repeat ( LTR ) - dependent, reporter gene expression was significantly impaired in cells derived from poly ( ADP - ribose ) polymerase - 1 ( PARP - 1 ) - knockout ( PARP - 1 - / - ) mice.

Precision: 50.00

Recall: 66.67

F1: 57.14

Score	Text
0.9774	NF - kappaB - dependent, as well as human immunodeficiency virus type - 1 ( HIV - 1 ) long terminal repeat ( LTR ) - dependent, reporter gene expression was significantly impaired in cells derived from poly ( ADP - ribose ) polymerase - 1 ( PARP - 1 ) - knockout Protein( PARP - 1 - / - ) mice.
0.8555	NF - kappaB - dependent, as well as human immunodeficiency virus type - 1 ( HIV - 1 ) long terminal repeat ( LTR ) - dependent, reporter gene expression was significantly impaired in cells derived from Proteinpoly ( ADP - ribose ) polymerase - 1 ( PARP - 1 ) - knockout ( PARP - 1 - / - ) mice.
0.9747	NF - kappaB - dependent, as well as human immunodeficiency virus type - 1 ( HIV - 1 ) long terminal repeat ( LTR ) - dependent, reporter gene expression was significantly impaired in cells derived from poly ( ADP - ribose ) polymerase - 1 ( PARP - 1 ) - knockout Protein( PARP - 1 - / - ) mice.
0.9455	NF - kappaB - dependent, as well as human immunodeficiency virus type - 1 ( HIV - 1 ) long terminal repeat ( LTR ) - dependent, reporter gene expression was significantly impaired in cells derived from poly ( ADP - ribose ) polymerase - 1 ( PARP - 1 ) - knockout ( PARP - 1 Protein- / - ) mice.
	NF - kappaB - dependent, as well as human immunodeficiency virus type - 1 ( HIV - 1 ) long terminal repeat ( LTR ) - dependent, reporter gene expression was significantly impaired in cells derived from poly ( ADP - ribose ) polymerase - 1 Protein( PARP - 1 ) - knockout ( PARP - 1 - / - ) mice.

(1097)

In addition, the level of protein acetylation was markedly lower in PARP - 1 - / - cells than control ( PARP - 1 + / + ) cells.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9983	In addition, the level of protein acetylation was markedly lower in ProteinPARP - 1 - / - cells than control ( PARP - 1 + / + ) cells.
0.9866	In addition, the level of protein acetylation was markedly lower in PARP - 1 - / - cells than control Protein( PARP - 1 + / + ) cells.
0.5370	In addition, the level of protein Acetylationacetylation was markedly lower in PARP - 1 - / - cells than control ( PARP - 1 + / + ) cells.

(1098)

Surprisingly, the expression levels of histone acetyltransferases ( HATs ), p300, cAMP response element - binding protein - binding protein ( CBP ), and p300 / CBP - associated factor ( PCAF ), were significantly reduced in PARP - 1 - / - cells, as compared with PARP - 1 + / + cells.

Precision: 88.89

Recall: 100.00

F1: 94.12

Score	Text
0.9995	Surprisingly, the expression levels of histone acetyltransferases ( HATs ), Proteinp300 , cAMP response element - binding protein - binding protein ( CBP ), and p300 / CBP - associated factor ( PCAF ), were significantly reduced in PARP - 1 - / - cells, as compared with PARP - 1 + / + cells.
0.9991	Surprisingly, the expression levels of histone acetyltransferases ( HATs ), p300, cAMP response element - binding protein - binding protein ( CBP ), and p300 / CBP - associated factor ( PCAF ), were significantly reduced in ProteinPARP - 1 - / - cells, as compared with PARP - 1 + / + cells.
0.9990	Surprisingly, the expression levels of histone acetyltransferases ( HATs ), p300, cAMP response element - binding protein - binding protein ( CBP ), and p300 / CBP - associated factor ( PCAF ), were significantly reduced in PARP - 1 - / - cells, as compared with ProteinPARP - 1 + / + cells.
0.9936	Surprisingly, the expression levels of histone acetyltransferases ( HATs ), p300, cAMP response element - binding protein - binding protein ( CBP ), and p300 / CBP - associated factor Protein( PCAF ) , were significantly reduced in PARP - 1 - / - cells, as compared with PARP - 1 + / + cells.
0.9929	Surprisingly, the expression levels of Proteinhistone acetyltransferases ( HATs ), p300, cAMP response element - binding protein - binding protein ( CBP ), and p300 / CBP - associated factor ( PCAF ), were significantly reduced in PARP - 1 - / - cells, as compared with PARP - 1 + / + cells.
0.9807	Surprisingly, the expression levels of histone acetyltransferases ( HATs ), p300, cAMP response element - binding protein - binding protein Protein( CBP ) , and p300 / CBP - associated factor ( PCAF ), were significantly reduced in PARP - 1 - / - cells, as compared with PARP - 1 + / + cells.
0.9652	Surprisingly, the expression levels of histone acetyltransferases ( HATs ), p300, cAMP response element - binding protein - binding protein ( CBP ), and Proteinp300 / CBP - associated factor ( PCAF ), were significantly reduced in PARP - 1 - / - cells, as compared with PARP - 1 + / + cells.
0.8667	Surprisingly, the expression levels of histone acetyltransferases ( HATs ), p300, ProteincAMP response element - binding protein - binding protein ( CBP ), and p300 / CBP - associated factor ( PCAF ), were significantly reduced in PARP - 1 - / - cells, as compared with PARP - 1 + / + cells.
0.5825	Surprisingly, the expression levels of histone acetyltransferases ( HATs ), p300, cAMP response element - binding protein - binding protein ( CBP ), and Proteinp300 / CBP - associated factor ( PCAF ) , were significantly reduced in PARP - 1 - / - cells, as compared with PARP - 1 + / + cells.

(1099)

These results suggest that PARP - 1 is required for the proper expression of particular HATs.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	These results suggest that ProteinPARP - 1 is required for the proper expression of particular HATs.

(1100)

Since p300 and CBP are coactivators of NF - kappaB, we propose here that PARP - 1 participates in NF - kappaB - dependent transcription by means of maintaining the expression of HATs.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	Since p300 and CBP are coactivators of NF - kappaB, we propose here that ProteinPARP - 1 participates in NF - kappaB - dependent transcription by means of maintaining the expression of HATs.
0.9989	Since p300 and ProteinCBP are coactivators of NF - kappaB, we propose here that PARP - 1 participates in NF - kappaB - dependent transcription by means of maintaining the expression of HATs.
0.9982	Since Proteinp300 and CBP are coactivators of NF - kappaB, we propose here that PARP - 1 participates in NF - kappaB - dependent transcription by means of maintaining the expression of HATs.

(1101)

The ITIM - bearing CLECSF6 ( DCIR ) is down - modulated in neutrophils by neutrophil activating agents.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9693	The ITIM - bearing ProteinCLECSF6 ( DCIR ) is down - modulated in neutrophils by neutrophil activating agents.
0.9686	The ITIM - bearing CLECSF6 Protein( DCIR ) is down - modulated in neutrophils by neutrophil activating agents.

(1102)

The recently discovered CLECSF6 protein displays the features of a receptor involved in the down - modulation of leukocyte activation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	The recently discovered ProteinCLECSF6 protein displays the features of a receptor involved in the down - modulation of leukocyte activation.

(1103)

Although CLECSF6 has been the focus of the interest of many researchers lately, a Western blot characterization of the protein is still lacking.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9984	Although ProteinCLECSF6 has been the focus of the interest of many researchers lately, a Western blot characterization of the protein is still lacking.

(1104)

This highly reduces our ability to gain full knowledge of the biological relevance of this protein in cell responses.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1105)

We produced two rabbit polyclonal antisera that detected a glycosylated protein at approximately 35kDa in neutrophils.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1106)

Four different CLECSF6 mRNA species have been discovered to date.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	Four different ProteinCLECSF6 mRNA species have been discovered to date.

(1107)

When deglycosylated, the protein displayed the molecular weight expected for the longest CLECSF6 form.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	When deglycosylated, the protein displayed the molecular weight expected for the longest ProteinCLECSF6 form.
0.4936	When Deglycosylationdeglycosylated , the protein displayed the molecular weight expected for the longest CLECSF6 form.

(1108)

Neutrophil membrane fractions were strongly enriched in the protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1109)

We showed a down - modulation of the expression of this protein in neutrophils treated with granulocyte - macrophage - colony stimulating factor ( GM - CSF ), tumor necrosis factor ( TNF - alpha ), lipopolysaccharide ( LPS ), and interleukin ( IL ) - 4.

Precision: 83.33

Recall: 100.00

F1: 90.91

Score	Text
0.9869	We showed a down - modulation of the expression of this protein in neutrophils treated with granulocyte - macrophage - colony stimulating factor ( GM - CSF ), tumor necrosis factor Protein( TNF - alpha ) , lipopolysaccharide ( LPS ), and interleukin ( IL ) - 4.
0.9723	We showed a down - modulation of the expression of this protein in neutrophils treated with granulocyte - macrophage - colony stimulating factor Protein( GM - CSF ) , tumor necrosis factor ( TNF - alpha ), lipopolysaccharide ( LPS ), and interleukin ( IL ) - 4.
0.9241	We showed a down - modulation of the expression of this protein in neutrophils treated with granulocyte - macrophage - colony stimulating factor ( GM - CSF ), tumor necrosis factor ( TNF - alpha ), lipopolysaccharide ( LPS ), and Proteininterleukin ( IL ) - 4 .
0.9057	We showed a down - modulation of the expression of this protein in neutrophils treated with Proteingranulocyte - macrophage - colony stimulating factor ( GM - CSF ), tumor necrosis factor ( TNF - alpha ), lipopolysaccharide ( LPS ), and interleukin ( IL ) - 4.
0.7893	We showed a down - modulation of the expression of this protein in neutrophils treated with granulocyte - macrophage - colony stimulating factor ( GM - CSF ), Proteintumor necrosis factor ( TNF - alpha ), lipopolysaccharide ( LPS ), and interleukin ( IL ) - 4.
0.8551	We showed a down - modulation of the expression of this protein in neutrophils treated with granulocyte - macrophage - colony stimulating factor ( GM - CSF ), tumor necrosis factor ( TNF - alpha ), lipopolysaccharide ( LPS ), and interleukin Protein( IL ) - 4 .

(1110)

This work supports the hypothesis that CLECSF6 is involved in the control of inflammation in neutrophils.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	This work supports the hypothesis that ProteinCLECSF6 is involved in the control of inflammation in neutrophils.

(1111)

Epigenetic switch from posttranscriptional to transcriptional silencing is correlated with promoter hypermethylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1112)

Changes in the distribution of methylcytosine residues along a transgene locus of tobacco ( Nicotiana tabacum ) in relation to the type of gene silencing were studied in parental plant leaves, calli, and regenerated plants derived thereof.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1113)

Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine methylation restricted to the 3'end and center part of the transcribed region.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9613	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing ProteinnptII ( neomycin phosphotransferase II ) transgene and cytosine methylation restricted to the 3'end and center part of the transcribed region.
0.9420	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine DNA_methylationmethylation restricted to the 3'end and center part of the transcribed region.
0.7816	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine methylation restricted to the Entity3'end and center part of the transcribed region.
0.7225	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine methylation restricted to the 3'end and Entitycenter part of the transcribed region.
0.5416	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII Protein( neomycin phosphotransferase II ) transgene and cytosine methylation restricted to the 3'end and center part of the transcribed region.
0.7828	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine methylation restricted to the 3 ' Entityend and center part of the transcribed region.
0.7353	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine methylation restricted to the Entity3 ' end and center part of the transcribed region.
0.7227	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine methylation restricted to the 3'end and Entitycenter part of the transcribed region.
0.5820	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing ProteinnptII ( neomycin phosphotransferase II ) transgene and cytosine methylation restricted to the 3'end and center part of the transcribed region.
0.5382	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine methylation restricted to the 3'end and center part of the Entitytranscribed region .

(1114)

Here, we report that with an increasing number of cell cycles, DNA methylation changes gradually, and methylation is introduced into the promoter during cell culture and more slowly in vegetatively propagated plants.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.6076	Here, we report that with an increasing number of cell cycles, DNA methylation changes gradually, and methylation is introduced into the Entitypromoter during cell culture and more slowly in vegetatively propagated plants.

(1115)

After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical ( CG and CNG ) sites was almost complete within the 5'end of the nptII - transcribed region and the 35S promoter.

Precision: 44.44

Recall: 100.00

F1: 61.54

Score	Text
0.9740	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine DNA_methylationmethylation of symmetrical ( CG and CNG ) sites was almost complete within the 5'end of the nptII - transcribed region and the 35S promoter.
0.9654	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical ( CG and CNG ) sites was almost complete within the 5'end of the ProteinnptII - transcribed region and the 35S promoter.
0.9293	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical ( CG and CNG ) sites was almost complete within the 5'end of the nptII - transcribed region and the Entity35S promoter .
0.8839	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical ( CG and CNG ) sites was almost complete within the Entity5'end of the nptII - transcribed region and the 35S promoter.
0.9458	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical ( CG and CNG ) sites was almost complete within the 5'end of the nptII - transcribed region and the 35S Entitypromoter .
0.9054	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical ( CG and CNG ) sites was almost complete within the Entity5 ' end of the nptII - transcribed region and the 35S promoter.
0.8944	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical ( CG and CNG ) sites was almost complete within the 5'end of the nptII - transcribed region and the Entity35S promoter.
0.6277	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical ( CG and CNG ) sites was almost complete within the 5 ' Entityend of the nptII - transcribed region and the 35S promoter.
0.5728	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical ( CG and CNG ) sites was almost complete within the 5'end of the nptII - transcribed Entityregion and the 35S promoter.

(1116)

Further, methylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of methylation was significantly reduced in the 3'end of the transcribed region when compared with locus 1.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9692	Further, DNA_methylationmethylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of methylation was significantly reduced in the 3'end of the transcribed region when compared with locus 1.
0.8032	Further, methylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of methylation was significantly reduced in the 3'end of the Entitytranscribed region when compared with locus 1.
0.7896	Further, methylation of nonsymmetrical sites appeared de novo in the Entitypromoter , whereas this type of methylation was significantly reduced in the 3'end of the transcribed region when compared with locus 1.
0.9536	Further, methylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of DNA_methylationmethylation was significantly reduced in the 3'end of the transcribed region when compared with locus 1.
0.6352	Further, methylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of methylation was significantly reduced in the 3'end of the Entitytranscribed region when compared with locus 1.
0.5083	Further, methylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of methylation was significantly reduced in the Entity3 ' end of the transcribed region when compared with locus 1.

(1117)

The newly established epigenetic patterns were stably transmitted from calli into regenerated plants and their progeny.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1118)

The protein and steady - state RNA levels remained low in locus 1E, whereas with nuclear run - on assays, no detectable amounts of primary transcripts were found along the nptII gene, indicating that the methylated promoter became inactivated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	The protein and steady - state RNA levels remained low in locus 1E, whereas with nuclear run - on assays, no detectable amounts of primary transcripts were found along the ProteinnptII gene, indicating that the methylated promoter became inactivated.
0.9927	The protein and steady - state RNA levels remained low in locus 1E, whereas with nuclear run - on assays, no detectable amounts of primary transcripts were found along the nptII gene, indicating that the methylated Entitypromoter became inactivated.
0.9706	The protein and steady - state RNA levels remained low in locus 1E, whereas with nuclear run - on assays, no detectable amounts of primary transcripts were found along the nptII gene, indicating that the DNA_methylationmethylated promoter became inactivated.

(1119)

The results suggest that a switch between posttranscriptional and transcriptional gene silencing could be a mechanism leading to irrevocable shut down of gene expression within a finite number of generations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1120)

Frequent methylation of p16INK4A and p14ARF genes implicated in the evolution of chronic myeloid leukaemia from its chronic to accelerated phase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9968	Frequent methylation of p16INK4A and Proteinp14ARF genes implicated in the evolution of chronic myeloid leukaemia from its chronic to accelerated phase.
0.9966	Frequent methylation of Proteinp16INK4A and p14ARF genes implicated in the evolution of chronic myeloid leukaemia from its chronic to accelerated phase.
0.8214	Frequent DNA_methylationmethylation of p16INK4A and p14ARF genes implicated in the evolution of chronic myeloid leukaemia from its chronic to accelerated phase.

(1121)

The frequency and mechanism of p16 ( INK4A ) and p14 ( ARF ) gene alterations were studied in cell samples from 30 patients with Philadelphia ( Ph ) chromosome - positive chronic myeloid leukaemia ( CML ), both at diagnosis and at the onset of the accelerated phase ( AP ) of the disease.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9971	The frequency and mechanism of p16 ( INK4A ) and Proteinp14 ( ARF ) gene alterations were studied in cell samples from 30 patients with Philadelphia ( Ph ) chromosome - positive chronic myeloid leukaemia ( CML ), both at diagnosis and at the onset of the accelerated phase ( AP ) of the disease.
0.9964	The frequency and mechanism of Proteinp16 ( INK4A ) and p14 ( ARF ) gene alterations were studied in cell samples from 30 patients with Philadelphia ( Ph ) chromosome - positive chronic myeloid leukaemia ( CML ), both at diagnosis and at the onset of the accelerated phase ( AP ) of the disease.

(1122)

No alterations in the p16 ( INK4A ) or p14 ( ARF ) genes were found in any of the chronic phase ( CP ) samples.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9950	No alterations in the Proteinp16 ( INK4A ) or p14 ( ARF ) genes were found in any of the chronic phase ( CP ) samples.
0.9935	No alterations in the p16 ( INK4A ) or Proteinp14 ( ARF ) genes were found in any of the chronic phase ( CP ) samples.

(1123)

DNA sequencing analyses detected p16 ( INK4A ) or p14 ( ARF ) mutations in 17 AP samples.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9970	DNA sequencing analyses detected Proteinp16 ( INK4A ) or p14 ( ARF ) mutations in 17 AP samples.
0.9970	DNA sequencing analyses detected p16 ( INK4A ) or Proteinp14 ( ARF ) mutations in 17 AP samples.

(1124)

All mutations were heterozygous without loss of the other allele.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1125)

Aberrant methylation of the p16 ( INK4A ) or p14 ( ARF ) promoters was found in 14 of 30 AP samples.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9930	Aberrant DNA_methylationmethylation of the p16 ( INK4A ) or p14 ( ARF ) promoters was found in 14 of 30 AP samples.
0.9839	Aberrant methylation of the p16 ( INK4A ) or Proteinp14 ( ARF ) promoters was found in 14 of 30 AP samples.
0.9797	Aberrant methylation of the Proteinp16 ( INK4A ) or p14 ( ARF ) promoters was found in 14 of 30 AP samples.
0.9729	Aberrant methylation of the p16 ( INK4A ) or p14 ( ARF ) Entitypromoters was found in 14 of 30 AP samples.

(1126)

The most common situation was the simultaneous methylation of both promoters.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9765	The most common situation was the simultaneous methylation of both Entitypromoters .
0.9643	The most common situation was the simultaneous DNA_methylationmethylation of both promoters.

(1127)

Our data indicate that p16 ( INK4A ) and p14 ( ARF ) are primary targets for inactivation by promoter methylation in the acceleration of CML.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9933	Our data indicate that p16 ( INK4A ) and Proteinp14 ( ARF ) are primary targets for inactivation by promoter methylation in the acceleration of CML.
0.9919	Our data indicate that Proteinp16 ( INK4A ) and p14 ( ARF ) are primary targets for inactivation by promoter methylation in the acceleration of CML.
0.9915	Our data indicate that p16 ( INK4A ) and p14 ( ARF ) are primary targets for inactivation by Entitypromoter methylation in the acceleration of CML.
0.9585	Our data indicate that p16 ( INK4A ) and p14 ( ARF ) are primary targets for inactivation by promoter DNA_methylationmethylation in the acceleration of CML.

(1128)

Transcriptional silencing of the p16 ( INK4A ) and p14 ( ARF ) genes may be important in the conversion of CML from the CP to the AP.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9957	Transcriptional silencing of the Proteinp16 ( INK4A ) and p14 ( ARF ) genes may be important in the conversion of CML from the CP to the AP.
0.9926	Transcriptional silencing of the p16 ( INK4A ) and Proteinp14 ( ARF ) genes may be important in the conversion of CML from the CP to the AP.

(1129)

mSin3A / histone deacetylase 2 - and PRMT5 - containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad.

Precision: 85.71

Recall: 100.00

F1: 92.31

Score	Text
0.9996	mSin3A / histone deacetylase 2 - and PRMT5 - containing Brg1 complex is involved in transcriptional repression of the Myc target gene Proteincad .
0.9996	mSin3A / histone deacetylase 2 - and PRMT5 - containing Brg1 complex is involved in transcriptional repression of the ProteinMyc target gene cad.
0.9763	ProteinmSin3A / histone deacetylase 2 - and PRMT5 - containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad.
0.9317	mSin3A / histone deacetylase 2 - and PRMT5 - containing ProteinBrg1 complex is involved in transcriptional repression of the Myc target gene cad.
0.7093	ProteinmSin3A / histone deacetylase 2 - and PRMT5 - containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad.
0.6314	mSin3A / histone deacetylase 2 - and ProteinPRMT5 - containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad.
0.7867	mSin3A / histone deacetylase 2 - and ProteinPRMT5 - containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad.

(1130)

The role of hSWI / SNF complexes in transcriptional activation is well characterized ; however, little is known about their function in transcriptional repression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1131)

We have previously shown that subunits of the mSin3A / histone deacetylase 2 ( HDAC2 ) corepressor complex copurify with hSWI / SNF complexes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9715	We have previously shown that subunits of the mSin3A / histone deacetylase 2 Protein( HDAC2 ) corepressor complex copurify with hSWI / SNF complexes.
0.9288	We have previously shown that subunits of the ProteinmSin3A / histone deacetylase 2 ( HDAC2 ) corepressor complex copurify with hSWI / SNF complexes.
0.6487	We have previously shown that subunits of the ProteinmSin3A / histone deacetylase 2 ( HDAC2 ) corepressor complex copurify with hSWI / SNF complexes.

(1132)

Here we show that the type II arginine - specific methyltransferase PRMT5, which is involved in cyclin E repression, can be found in association with Brg1 and hBrm - based hSWI / SNF complexes.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9999	Here we show that the type II arginine - specific methyltransferase ProteinPRMT5 , which is involved in cyclin E repression, can be found in association with Brg1 and hBrm - based hSWI / SNF complexes.
0.9998	Here we show that the type II arginine - specific methyltransferase PRMT5, which is involved in cyclin E repression, can be found in association with ProteinBrg1 and hBrm - based hSWI / SNF complexes.
0.9986	Here we show that the type II arginine - specific methyltransferase PRMT5, which is involved in cyclin E repression, can be found in association with Brg1 and ProteinhBrm - based hSWI / SNF complexes.
0.7728	Here we show that the type II arginine - specific methyltransferase PRMT5, which is involved in Proteincyclin E repression, can be found in association with Brg1 and hBrm - based hSWI / SNF complexes.

(1133)

We also show that hSWI / SNF - associated PRMT5 can methylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.

Precision: 77.78

Recall: 77.78

F1: 77.78

Score	Text
0.9889	We also show that hSWI / SNF - associated ProteinPRMT5 can methylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.
0.9673	We also show that hSWI / SNF - associated PRMT5 can methylate hypoacetylated histones H3 and ProteinH4 more efficiently than hyperacetylated histones H3 and H4.
0.9507	We also show that hSWI / SNF - associated PRMT5 can methylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and ProteinH4 .
0.8992	We also show that hSWI / SNF - associated PRMT5 can methylate hypoacetylated Proteinhistones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.
0.8981	We also show that hSWI / SNF - associated PRMT5 can methylate hypoacetylated histones H3 and H4 more efficiently than Acetylationhyperacetylated histones H3 and H4.
0.8859	We also show that hSWI / SNF - associated PRMT5 can methylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated Proteinhistones H3 and H4.
0.5522	We also show that hSWI / SNF - associated PRMT5 can Methylationmethylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.
0.8602	We also show that hSWI / SNF - associated PRMT5 can methylate Acetylationhypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.
0.4086	We also show that hSWI / SNF - associated PRMT5 can Acetylationmethylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.
	We also show that hSWI / SNF - associated PRMT5 can Catalysismethylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.
	We also show that hSWI / SNF - associated PRMT5 can methylate Deacetylationhypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.

(1134)

Protein - protein interaction studies indicate that PRMT5 and mSin3A interact with the same hSWI / SNF subunits as those targeted by c - Myc.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Protein - protein interaction studies indicate that PRMT5 and mSin3A interact with the same hSWI / SNF subunits as those targeted by Proteinc - Myc .
0.9997	Protein - protein interaction studies indicate that ProteinPRMT5 and mSin3A interact with the same hSWI / SNF subunits as those targeted by c - Myc.
0.9996	Protein - protein interaction studies indicate that PRMT5 and ProteinmSin3A interact with the same hSWI / SNF subunits as those targeted by c - Myc.

(1135)

These observations prompted us to examine the expression profile of the c - Myc target genes, carbamoyl - phosphate synthase - aspartate carbamoyltransferase - dihydroorotase ( cad ) and nucleolin ( nuc ).

Precision: 77.78

Recall: 100.00

F1: 87.50

Score	Text
0.9995	These observations prompted us to examine the expression profile of the Proteinc - Myc target genes, carbamoyl - phosphate synthase - aspartate carbamoyltransferase - dihydroorotase ( cad ) and nucleolin ( nuc ).
0.9893	These observations prompted us to examine the expression profile of the c - Myc target genes, carbamoyl - phosphate synthase - aspartate carbamoyltransferase - dihydroorotase Protein( cad ) and nucleolin ( nuc ).
0.9884	These observations prompted us to examine the expression profile of the c - Myc target genes, carbamoyl - phosphate synthase - aspartate carbamoyltransferase - dihydroorotase ( cad ) and nucleolin Protein( nuc ) .
0.8008	These observations prompted us to examine the expression profile of the c - Myc target genes, Proteincarbamoyl - phosphate synthase - aspartate carbamoyltransferase - dihydroorotase ( cad ) and nucleolin ( nuc ).
0.7198	These observations prompted us to examine the expression profile of the c - Myc target genes, carbamoyl - phosphate synthase - aspartate carbamoyltransferase - dihydroorotase ( cad ) and Proteinnucleolin ( nuc ).
0.6112	These observations prompted us to examine the expression profile of the c - Myc target genes, carbamoyl - phosphate synthase - aspartate Proteincarbamoyltransferase - dihydroorotase ( cad ) and nucleolin ( nuc ).
0.5662	These observations prompted us to examine the expression profile of the c - Myc target genes, carbamoyl - phosphate Proteinsynthase - aspartate carbamoyltransferase - dihydroorotase ( cad ) and nucleolin ( nuc ).
0.7418	These observations prompted us to examine the expression profile of the c - Myc target genes, Proteincarbamoyl - phosphate synthase - aspartate carbamoyltransferase - dihydroorotase ( cad ) and nucleolin ( nuc ).
0.5126	These observations prompted us to examine the expression profile of the c - Myc target genes, carbamoyl - phosphate Proteinsynthase - aspartate carbamoyltransferase - dihydroorotase ( cad ) and nucleolin ( nuc ).

(1136)

We found that cad repression is altered in cells that express inactive Brg1 and in cells treated with the HDAC inhibitor depsipeptide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	We found that Proteincad repression is altered in cells that express inactive Brg1 and in cells treated with the HDAC inhibitor depsipeptide.
0.9997	We found that cad repression is altered in cells that express inactive ProteinBrg1 and in cells treated with the HDAC inhibitor depsipeptide.

(1137)

Using chromatin immunoprecipitation assays, we found that Brg1, mSin3A, HDAC2, and PRMT5 are directly recruited to the cad promoter.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Using chromatin immunoprecipitation assays, we found that Brg1, mSin3A, HDAC2, and ProteinPRMT5 are directly recruited to the cad promoter.
0.9996	Using chromatin immunoprecipitation assays, we found that Brg1, mSin3A, ProteinHDAC2 , and PRMT5 are directly recruited to the cad promoter.
0.9995	Using chromatin immunoprecipitation assays, we found that Brg1, ProteinmSin3A , HDAC2, and PRMT5 are directly recruited to the cad promoter.
0.9994	Using chromatin immunoprecipitation assays, we found that ProteinBrg1 , mSin3A, HDAC2, and PRMT5 are directly recruited to the cad promoter.
0.9967	Using chromatin immunoprecipitation assays, we found that Brg1, mSin3A, HDAC2, and PRMT5 are directly recruited to the Proteincad promoter.

(1138)

These results suggest that hSWI / SNF complexes, through their ability to interact with activator and repressor proteins, control expression of genes involved in cell growth and proliferation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1139)

Centromere silencing and function in fission yeast is governed by the amino terminus of histone H3.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9651	Centromere silencing and function in fission yeast is governed by the amino terminus of Proteinhistone H3 .

(1140)

BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by hypoacetylation of histones H3 and H4 and methylation of lysine 9 of histone H3 ( K9 - MeH3 ).

Precision: 87.50

Recall: 87.50

F1: 87.50

Score	Text
0.9866	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by hypoacetylation of histones H3 and ProteinH4 and methylation of lysine 9 of histone H3 ( K9 - MeH3 ).
0.9522	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by hypoacetylation of histones H3 and H4 and Methylationmethylation of lysine 9 of histone H3 ( K9 - MeH3 ).
0.9332	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by hypoacetylation of Proteinhistones H3 and H4 and methylation of lysine 9 of histone H3 ( K9 - MeH3 ).
0.9282	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by hypoacetylation of histones H3 and H4 and methylation of lysine 9 of Proteinhistone H3 ( K9 - MeH3 ).
0.9264	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by Acetylationhypoacetylation of histones H3 and H4 and methylation of lysine 9 of histone H3 ( K9 - MeH3 ).
0.8943	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by hypoacetylation of histones H3 and H4 and methylation of Entitylysine 9 of histone H3 ( K9 - MeH3 ).
0.5115	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by hypoacetylation of histones H3 and H4 and methylation of lysine 9 of histone H3 Entity( K9 - MeH3 ) .
0.5391	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by hypoacetylation of histones H3 and H4 and methylation of Entitylysine 9 of histone H3 ( K9 - MeH3 ).
	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by hypoacetylation of histones H3 and H4 and methylation of lysine 9 of histone H3 Protein( K9 - MeH3 ) .

(1141)

Perturbation of this underacetylated state by transient treatment with histone deacetylase inhibitors leads to defective centromere function, correlating with delocalization of the heterochromatin protein Swi6 / HP1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	Perturbation of this underacetylated state by transient treatment with histone deacetylase inhibitors leads to defective centromere function, correlating with delocalization of the heterochromatin protein ProteinSwi6 / HP1 .
0.9987	Perturbation of this underacetylated state by transient treatment with Proteinhistone deacetylase inhibitors leads to defective centromere function, correlating with delocalization of the heterochromatin protein Swi6 / HP1.

(1142)

Likewise, deletion of the K9 - MeH3 methyltransferase Clr4 / Suvar39 causes defective chromosome segregation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	Likewise, deletion of the K9 - MeH3 methyltransferase ProteinClr4 / Suvar39 causes defective chromosome segregation.
0.9893	Likewise, deletion of the ProteinK9 - MeH3 methyltransferase Clr4 / Suvar39 causes defective chromosome segregation.

(1143)

Here, we create fission yeast strains retaining one histone H3 and H4 gene ; the creation of these strains allows mutation of specific N - terminal tail residues and their role in centromeric silencing and chromosome stability to be investigated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	Here, we create fission yeast strains retaining one histone H3 and ProteinH4 gene ; the creation of these strains allows mutation of specific N - terminal tail residues and their role in centromeric silencing and chromosome stability to be investigated.
0.9899	Here, we create fission yeast strains retaining one Proteinhistone H3 and H4 gene ; the creation of these strains allows mutation of specific N - terminal tail residues and their role in centromeric silencing and chromosome stability to be investigated.

(1144)

RESULTS : Reduction of H3 / H4 gene dosage to one - third does not affect cell viability or heterochromatin formation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	RESULTS : Reduction of ProteinH3 / H4 gene dosage to one - third does not affect cell viability or heterochromatin formation.

(1145)

Mutation of lysines 9 or 14 or serine 10 within the amino terminus of histone H3 impairs centromere function, leading to defective chromosome segregation and Swi6 delocalization.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	Mutation of lysines 9 or 14 or serine 10 within the amino terminus of histone H3 impairs centromere function, leading to defective chromosome segregation and ProteinSwi6 delocalization.
0.9658	Mutation of lysines 9 or 14 or serine 10 within the amino terminus of Proteinhistone H3 impairs centromere function, leading to defective chromosome segregation and Swi6 delocalization.

(1146)

Surprisingly, silent centromeric chromatin does not require the conserved lysine 8 and 16 residues of histone H4.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9723	Surprisingly, silent centromeric chromatin does not require the conserved lysine 8 and 16 residues of Proteinhistone H4 .

(1147)

CONCLUSIONS : To date, mutation of conserved N - terminal residues in endogenous histone genes has only been performed in budding yeast, which lacks the Clr4 / Suvar39 histone methyltransferase and Swi6 / HP1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	CONCLUSIONS : To date, mutation of conserved N - terminal residues in endogenous histone genes has only been performed in budding yeast, which lacks the Clr4 / Suvar39 histone methyltransferase and ProteinSwi6 / HP1 .
0.9976	CONCLUSIONS : To date, mutation of conserved N - terminal residues in endogenous Proteinhistone genes has only been performed in budding yeast, which lacks the Clr4 / Suvar39 histone methyltransferase and Swi6 / HP1.
0.9228	CONCLUSIONS : To date, mutation of conserved N - terminal residues in endogenous histone genes has only been performed in budding yeast, which lacks the ProteinClr4 / Suvar39 histone methyltransferase and Swi6 / HP1.

(1148)

We demonstrate the importance of conserved residues within the histone H3 N terminus for the maintenance of centromeric heterochromatin in fission yeast.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9839	We demonstrate the importance of conserved residues within the Proteinhistone H3 N terminus for the maintenance of centromeric heterochromatin in fission yeast.

(1149)

In sharp contrast, mutation of two conserved lysines within the histone H4 tail has no impact on the integrity of centromeric heterochromatin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9493	In sharp contrast, mutation of two conserved lysines within the Proteinhistone H4 tail has no impact on the integrity of centromeric heterochromatin.

(1150)

Our data highlight the striking divergence between the histone tail requirements for the fission yeast and budding yeast silencing pathways.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9949	Our data highlight the striking divergence between the Proteinhistone tail requirements for the fission yeast and budding yeast silencing pathways.

(1151)

Thermal stability and aggregation of sulfolobus solfataricus beta - glycosidase are dependent upon the N - epsilon - methylation of specific lysyl residues : critical role of in vivo post - translational modifications.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9844	Thermal stability and aggregation of sulfolobus solfataricus Proteinbeta - glycosidase are dependent upon the N - epsilon - methylation of specific lysyl residues : critical role of in vivo post - translational modifications.
0.9442	Thermal stability and aggregation of sulfolobus solfataricus beta - glycosidase are dependent upon the N - epsilon - methylation of specific Entitylysyl residues : critical role of in vivo post - translational modifications.
0.6472	Thermal stability and aggregation of sulfolobus solfataricus beta - glycosidase are dependent upon the MethylationN - epsilon - methylation of specific lysyl residues : critical role of in vivo post - translational modifications.
0.8880	Thermal stability and aggregation of sulfolobus solfataricus beta - glycosidase are dependent upon the N - epsilon - methylation of specific Entitylysyl residues : critical role of in vivo post - translational modifications.

(1152)

Methylation in vivo is a post - translational modification observed in several organisms belonging to eucarya, bacteria, and archaea.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1153)

Although important implications of this modification have been demonstrated in several eucaryotes, its biological role in hyperthermophilic archaea is far from being understood.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1154)

The aim of this work is to clarify some effects of methylation on the properties of beta - glycosidase from Sulfolobus solfataricus, by a structural comparison between the native, methylated protein and its unmethylated counterpart, recombinantly expressed in Escherichia coli.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9784	The aim of this work is to clarify some effects of methylation on the properties of beta - glycosidase from Sulfolobus solfataricus, by a structural comparison between the native, Methylationmethylated protein and its unmethylated counterpart, recombinantly expressed in Escherichia coli.
0.9692	The aim of this work is to clarify some effects of methylation on the properties of Proteinbeta - glycosidase from Sulfolobus solfataricus, by a structural comparison between the native, methylated protein and its unmethylated counterpart, recombinantly expressed in Escherichia coli.
0.9608	The aim of this work is to clarify some effects of methylation on the properties of beta - glycosidase from Sulfolobus solfataricus, by a structural comparison between the native, methylated protein and its Methylationunmethylated counterpart, recombinantly expressed in Escherichia coli.
0.9157	The aim of this work is to clarify some effects of Methylationmethylation on the properties of beta - glycosidase from Sulfolobus solfataricus, by a structural comparison between the native, methylated protein and its unmethylated counterpart, recombinantly expressed in Escherichia coli.

(1155)

Analysis by Fourier transform infrared spectroscopy indicated similar secondary structure contents for the two forms of the protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1156)

However, the study of temperature perturbation by Fourier transform infrared spectroscopy and turbidimetry evidenced denaturation and aggregation events more pronounced in recombinant than in native beta - glycosidase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9969	However, the study of temperature perturbation by Fourier transform infrared spectroscopy and turbidimetry evidenced denaturation and aggregation events more pronounced in recombinant than in native Proteinbeta - glycosidase .

(1157)

Red Nile fluorescence analysis revealed significant differences of surface hydrophobicity between the two forms of the protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1158)

Unlike the native enzyme, which dissociated into SDS - resistant dimers upon exposure to the detergent, the recombinant enzyme partially dissociated into monomers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1159)

By electrospray mapping, the methylation sites of the native protein were identified.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1160)

A computational analysis of beta - glycosidase three - dimensional structure and comparisons with other proteins from S. solfataricus revealed analogies in the localization of methylation sites in terms of secondary structural elements and overall topology.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9915	A computational analysis of Proteinbeta - glycosidase three - dimensional structure and comparisons with other proteins from S. solfataricus revealed analogies in the localization of methylation sites in terms of secondary structural elements and overall topology.

(1161)

These observations suggest a role for the methylation of lysyl residues, located in selected domains, in the thermal stabilization of beta - glycosidase from S. solfataricus.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9913	These observations suggest a role for the methylation of lysyl residues, located in selected domains, in the thermal stabilization of Proteinbeta - glycosidase from S. solfataricus.
0.9567	These observations suggest a role for the Methylationmethylation of lysyl residues, located in selected domains, in the thermal stabilization of beta - glycosidase from S. solfataricus.
0.9143	These observations suggest a role for the methylation of Entitylysyl residues , located in selected domains, in the thermal stabilization of beta - glycosidase from S. solfataricus.
0.6708	These observations suggest a role for the methylation of Entitylysyl residues, located in selected domains, in the thermal stabilization of beta - glycosidase from S. solfataricus.

(1162)

Pathways for phosphatidylcholine biosynthesis in bacteria.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1163)

Phosphatidylcholine ( PC ) is the major membrane - forming phospholipid in eukaryotes with important structural and signalling functions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1164)

Although many prokaryotes lack PC, it can be found in significant amounts in membranes of rather diverse bacteria.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1165)

Two pathways for PC biosynthesis are known in bacteria, the methylation pathway and the phosphatidylcholine synthase ( PCS ) pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9632	Two pathways for PC biosynthesis are known in bacteria, the methylation pathway and the phosphatidylcholine synthase Protein( PCS ) pathway.
0.8586	Two pathways for PC biosynthesis are known in bacteria, the methylation pathway and the Proteinphosphatidylcholine synthase ( PCS ) pathway.

(1166)

In the methylation pathway, phosphatidylethanolamine is methylated three times to yield PC, in reactions catalysed by one or several phospholipid N - methyltransferases ( PMTs ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1167)

In the PCS pathway, choline is condensed directly with CDP - diacylglyceride to form PC in a reaction catalysed by PCS.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1168)

Using cell - free extracts, it was demonstrated that Sinorhizobium meliloti, Agrobacterium tumefaciens, Rhizobium leguminosarum, Bradyrhizobium japonicum, Mesorhizobium loti and Legionella pneumophila have both PMT and PCS activities.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9990	Using cell - free extracts, it was demonstrated that Sinorhizobium meliloti, Agrobacterium tumefaciens, Rhizobium leguminosarum, Bradyrhizobium japonicum, Mesorhizobium loti and Legionella pneumophila have both PMT and ProteinPCS activities.
0.9123	Using cell - free extracts, it was demonstrated that Sinorhizobium meliloti, Agrobacterium tumefaciens, Rhizobium leguminosarum, Bradyrhizobium japonicum, Mesorhizobium loti and Legionella pneumophila have both ProteinPMT and PCS activities.

(1169)

In addition, Rhodobacter sphaeroides has PMT activity and Brucella melitensis, Pseudomonas aeruginosa and Borrelia burgdorferi have PCS activities.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1170)

Genes from M. loti and L. pneumophila encoding a Pmt or a Pcs activity and the genes from P. aeruginosa and Borrelia burgdorferi responsible for Pcs activity have been identified.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	Genes from M. loti and L. pneumophila encoding a ProteinPmt or a Pcs activity and the genes from P. aeruginosa and Borrelia burgdorferi responsible for Pcs activity have been identified.
0.9987	Genes from M. loti and L. pneumophila encoding a Pmt or a Pcs activity and the genes from P. aeruginosa and Borrelia burgdorferi responsible for ProteinPcs activity have been identified.
0.9986	Genes from M. loti and L. pneumophila encoding a Pmt or a ProteinPcs activity and the genes from P. aeruginosa and Borrelia burgdorferi responsible for Pcs activity have been identified.

(1171)

Based on these functional assignments and on genomic data, one might predict that if bacteria contain PC as a membrane lipid, they usually possess both bacterial pathways for PC biosynthesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1172)

However, important pathogens such as Brucella melitensis, P. aeruginosa and Borrelia burgdorferi seem to be exceptional as they possess only the PCS pathway for PC formation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	However, important pathogens such as Brucella melitensis, P. aeruginosa and Borrelia burgdorferi seem to be exceptional as they possess only the ProteinPCS pathway for PC formation.

(1173)

Rapid screening for S - adenosylmethionine - dependent methylation products by enzyme - transferred isotope patterns analysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1174)

We report here an isotopic labeling and mass spectrometric method to rapidly identify S - adenosylmethionine ( AdoMet ) - dependent methylation products.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1175)

In the presence of CH ( 3 ) - and CD ( 3 ) - labeled AdoMet, a methyl transfer product appears as a doublet separated by 3 Da in a mass spectrum, while other compounds show their normal isotopic distribution.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1176)

Based on this unique isotopic pattern, methylation product ( s ) can be easily detected even from a mixture of cellular components.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1177)

To validate our method, the product of human thiopurine methyltransferase ( TPMT, EC 2. 1. 1. 67 ) has been successfully identified from both an in vitro assay and a whole - cell assay.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9858	To validate our method, the product of human thiopurine methyltransferase Protein( TPMT , EC 2. 1. 1. 67 ) has been successfully identified from both an in vitro assay and a whole - cell assay.
0.9026	To validate our method, the product of human Proteinthiopurine methyltransferase ( TPMT, EC 2. 1. 1. 67 ) has been successfully identified from both an in vitro assay and a whole - cell assay.

(1178)

This method is generally applicable to AdoMet - dependent transmethylation and other group - transfer reactions, and constitutes the first example of a general strategy of enzyme - transferred isotope patterns ( ETIPs ) analysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1179)

Subunit - specific sulphation of oligosaccharides relating to charge - heterogeneity in porcine lutrophin isoforms.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1180)

Lutrophin ( LH ) consists of an array of isoforms with different charges and bioactivities.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1181)

This study was undertaken to clarify specifically how oligosaccharides of alpha and beta subunits contribute to LH isoform charges.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1182)

Porcine LH ( pLH ) was separated into four isoforms by isoelectric focusing ( IEF ), followed by subunit isolation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1183)

Their oligosaccharides were released by hydrazinolysis, labelled by reduction with NaB3H4, and fractionated by HPLC with a Mono Q column into five populations differing in the number of sulphate ( S ) and sialic acid ( N ) residues, designated as Neutral, N - 1, S - 1, S - N and S - 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1184)

Oligosaccharides were predominantly sulphated ( S - 1 and S - 2 ) and infrequently sialylated ( N - 1 and S - N ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1185)

Further analysis, including concanavalin A ( Con A ) affinity chromatography, desialylation, desulphation, sequential exoglycosidase digestion and methylation, clarified the structures of the acidic oligosaccharides.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9297	Further analysis, including concanavalin A Protein( Con A ) affinity chromatography, desialylation, desulphation, sequential exoglycosidase digestion and methylation, clarified the structures of the acidic oligosaccharides.
0.9002	Further analysis, including Proteinconcanavalin A ( Con A ) affinity chromatography, desialylation, desulphation, sequential exoglycosidase digestion and methylation, clarified the structures of the acidic oligosaccharides.
0.7227	Further analysis, including concanavalin A Protein( Con A ) affinity chromatography, desialylation, desulphation, sequential exoglycosidase digestion and methylation, clarified the structures of the acidic oligosaccharides.
0.5534	Further analysis, including concanavalin A ( Con ProteinA ) affinity chromatography, desialylation, desulphation, sequential exoglycosidase digestion and methylation, clarified the structures of the acidic oligosaccharides.

(1186)

All were of the biantennary complex type.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1187)

Their two peripheral branches were SO4 - 4GalNAc beta 1 - 4Glc - NAc and GalNAc beta 1 - 4GlcNAc or GlcNAc in S - 1, SO4 - 4GalNAc beta 1 - 4GlcNAc and Sia alpha 2 - 6Gal beta 1 - 4GlcNAc in S - N, and ( SO4 - 4GalNAc beta 1 - 4GlcNAc ) 2 in S - 2 ( where GalNAc is N - acetylgalactosamine and GlcNAc is N - acetylglucosamine ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1188)

Ten percent of S - 1 and of S - N had a bisecting GlcNAc residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1189)

Sulphate residues occurred in nearly the same amount for both subunits ; however, the alpha and beta subunits were sulphated differently.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1190)

S - 1 predominated in the alpha subunit, while S - 1 and S - 2 were major components in the beta subunit. ( ABSTRACT TRUNCATED AT 250 WORDS )

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1191)

The MTHFR 677C > T polymorphism is associated with an increased risk of hepatocellular carcinoma in patients with alcoholic cirrhosis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9942	The ProteinMTHFR 677C > T polymorphism is associated with an increased risk of hepatocellular carcinoma in patients with alcoholic cirrhosis.

(1192)

Methylenetetrahydrofolate reductase ( MTHFR ), a key enzyme in folate metabolism, plays a major role in the provision of methyl groups for DNA methylation and in the production of dTMP for DNA synthesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9879	Methylenetetrahydrofolate reductase Protein( MTHFR ) , a key enzyme in folate metabolism, plays a major role in the provision of methyl groups for DNA methylation and in the production of dTMP for DNA synthesis.
0.9147	ProteinMethylenetetrahydrofolate reductase ( MTHFR ), a key enzyme in folate metabolism, plays a major role in the provision of methyl groups for DNA methylation and in the production of dTMP for DNA synthesis.

(1193)

Different polymorphisms have been described for this enzyme, the most studied being the C677T, which has been shown to be associated with predisposition to colorectal cancer in patients who consume a high alcohol diet.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1194)

The aim of this study was to determine whether the MTHFR polymorphism is related to hepatocellular carcinoma ( HCC ) in patients with alcoholic cirrhosis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	The aim of this study was to determine whether the ProteinMTHFR polymorphism is related to hepatocellular carcinoma ( HCC ) in patients with alcoholic cirrhosis.

(1195)

MTHFR genotypes were determined in 300 liver transplant patients, 72 of whom had alcoholic cirrhosis with HCC and 122 of whom had alcoholic cirrhosis without HCC.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	ProteinMTHFR genotypes were determined in 300 liver transplant patients, 72 of whom had alcoholic cirrhosis with HCC and 122 of whom had alcoholic cirrhosis without HCC.

(1196)

The remaining patients were transplanted for HCC on normal liver ( n = 27 ) or viral cirrhosis with HCC ( n = 49 ) or without HCC ( n = 30 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1197)

We also tested 80 healthy subjects.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1198)

Among the group of patients transplanted for alcoholic cirrhosis, the frequency of MTHFR variants CC versus CT and TT was significantly higher in patients with HCC than in patients without macroscopic evidence of HCC ( P = 0. 02 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	Among the group of patients transplanted for alcoholic cirrhosis, the frequency of ProteinMTHFR variants CC versus CT and TT was significantly higher in patients with HCC than in patients without macroscopic evidence of HCC ( P = 0. 02 ).

(1199)

This difference was not observed between patients with and without HCC developed either on viral cirrhosis or on non - cirrhotic liver.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1200)

If we considered all the patients transplanted for HCC, the MTHFR CC genotype was significantly higher in patients who had developed HCC on alcoholic cirrhosis rather than on viral cirrhosis ( P = 0. 002 ) or on non - cirrhotic livers ( P = 0. 02 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9975	If we considered all the patients transplanted for HCC, the ProteinMTHFR CC genotype was significantly higher in patients who had developed HCC on alcoholic cirrhosis rather than on viral cirrhosis ( P = 0. 002 ) or on non - cirrhotic livers ( P = 0. 02 ).

(1201)

The relative risk for HCC in subjects with alcoholic cirrhosis and the CC genotype was 2. 03.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1202)

These results suggest that the MTHFR CC genotype increases the risk to develop HCC in patients who consume a high alcohol diet.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9920	These results suggest that the ProteinMTHFR CC genotype increases the risk to develop HCC in patients who consume a high alcohol diet.

(1203)

Mbd1 is recruited to both methylated and nonmethylated CpGs via distinct DNA binding domains.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	ProteinMbd1 is recruited to both methylated and nonmethylated CpGs via distinct DNA binding domains.

(1204)

MBD1 is a vertebrate methyl - CpG binding domain protein ( MBD ) that can bring about repression of methylated promoter DNA sequences.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	ProteinMBD1 is a vertebrate methyl - CpG binding domain protein ( MBD ) that can bring about repression of methylated promoter DNA sequences.

(1205)

Like other MBD proteins, MBD1 localizes to nuclear foci that in mice are rich in methyl - CpG.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	Like other MBD proteins, ProteinMBD1 localizes to nuclear foci that in mice are rich in methyl - CpG.

(1206)

In methyl - CpG - deficient mouse cells, however, Mbd1 remains localized to heterochromatic foci whereas other MBD proteins become dispersed in the nucleus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	In methyl - CpG - deficient mouse cells, however, ProteinMbd1 remains localized to heterochromatic foci whereas other MBD proteins become dispersed in the nucleus.

(1207)

We find that Mbd1a, a major mouse isoform, contains a CXXC domain ( CXXC - 3 ) that binds specifically to nonmethylated CpG, suggesting an explanation for methylation - independent localization.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	We find that ProteinMbd1a , a major mouse isoform, contains a CXXC domain ( CXXC - 3 ) that binds specifically to nonmethylated CpG, suggesting an explanation for methylation - independent localization.

(1208)

Transfection studies demonstrate that the CXXC - 3 domain indeed targets nonmethylated CpG sites in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1209)

Repression of nonmethylated reporter genes depends on the CXXC - 3 domain, whereas repression of methylated reporters requires the MBD.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1210)

Our findings indicate that MBD1 can interpret the CpG dinucleotide as a repressive signal in vivo regardless of its methylation status.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Our findings indicate that ProteinMBD1 can interpret the CpG dinucleotide as a repressive signal in vivo regardless of its methylation status.

(1211)

Recombinant human zona pellucida proteins ZP1, ZP2 and ZP3 co - expressed in a human cell line.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Recombinant human zona pellucida proteins ZP1, ZP2 and ProteinZP3 co - expressed in a human cell line.
0.9995	Recombinant human zona pellucida proteins ZP1, ProteinZP2 and ZP3 co - expressed in a human cell line.
0.9994	Recombinant human zona pellucida proteins ProteinZP1 , ZP2 and ZP3 co - expressed in a human cell line.

(1212)

AIM : To produce biologically active recombinant human ( rh ) ZP proteins in a human cell for use in sperm function tests.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1213)

METHODS : The human embryonic kidney cell line 293T was employed to produce rhZP1, rhZP2 and rhZP3 proteins individually and together by co - expression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	METHODS : The human embryonic kidney cell line 293T was employed to produce rhZP1, ProteinrhZP2 and rhZP3 proteins individually and together by co - expression.
0.9997	METHODS : The human embryonic kidney cell line 293T was employed to produce ProteinrhZP1 , rhZP2 and rhZP3 proteins individually and together by co - expression.
0.9994	METHODS : The human embryonic kidney cell line 293T was employed to produce rhZP1, rhZP2 and ProteinrhZP3 proteins individually and together by co - expression.

(1214)

Presence of these proteins in the culture medium and cell lysate was assessed by Western blotting analysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1215)

The effect of the recombinant proteins on the human AR was assessed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1216)

RESULTS : RhZP2 and rhZP3 were secreted into the culture medium, whereas rhZP1 was found only in the cell lysate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	RESULTS : RhZP2 and rhZP3 were secreted into the culture medium, whereas ProteinrhZP1 was found only in the cell lysate.
0.9995	RESULTS : RhZP2 and ProteinrhZP3 were secreted into the culture medium, whereas rhZP1 was found only in the cell lysate.
0.9993	RESULTS : ProteinRhZP2 and rhZP3 were secreted into the culture medium, whereas rhZP1 was found only in the cell lysate.

(1217)

Interestingly, when all zona pellucida proteins were co - expressed in the same cells, rhZP1 was also secreted into the culture medium.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Interestingly, when all zona pellucida proteins were co - expressed in the same cells, ProteinrhZP1 was also secreted into the culture medium.

(1218)

However, despite the presence of all three ZP proteins in sufficient concentration and evidence of heavy glycosylation on gel electrophoresis, biological activity to induce the AR was not observed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1219)

CONCLUSION : RhZP1, rhZP2 and rhZP3 were successfully expressed in the human embryonic kidney cell line 293T.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9992	CONCLUSION : RhZP1, ProteinrhZP2 and rhZP3 were successfully expressed in the human embryonic kidney cell line 293T.
0.9991	CONCLUSION : RhZP1, rhZP2 and ProteinrhZP3 were successfully expressed in the human embryonic kidney cell line 293T.
0.9983	CONCLUSION : ProteinRhZP1 , rhZP2 and rhZP3 were successfully expressed in the human embryonic kidney cell line 293T.

(1220)

It appears that an interaction amongst these proteins may be required for release of rhZP1 from the cell.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	It appears that an interaction amongst these proteins may be required for release of ProteinrhZP1 from the cell.

(1221)

Although this approach is not satisfactory for producing active human ZP proteins, it makes a significant contribution to the understanding of the structural and functional characteristics of the ZP proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1222)

Leu - 574 of human HIF - 1alpha is a molecular determinant of prolyl hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9983	Leu - 574 of human ProteinHIF - 1alpha is a molecular determinant of prolyl hydroxylation.

(1223)

Hypoxia - inducible factor ( HIF ) - 1alpha, a master regulator of oxygen homeostasis, regulates genes crucial for cell growth and survival.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
	ProteinHypoxia - inducible factor ( HIF ) - 1alpha , a master regulator of oxygen homeostasis, regulates genes crucial for cell growth and survival.

(1224)

In normoxia, HIF - 1alpha is constantly degraded via the ubiquitin - proteasome pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	In normoxia, ProteinHIF - 1alpha is constantly degraded via the ubiquitin - proteasome pathway.
0.9977	In normoxia, HIF - 1alpha is constantly degraded via the Proteinubiquitin - proteasome pathway.

(1225)

The von Hippel - Lindau ( VHL ) E3 ubiquitin ligase binds HIF - 1alpha through specific recognition of hydroxylated Pro - 402 or Pro - 564, both of which are modified by the oxygen - dependent HIF prolyl hydroxylases ( PHDs / HPHs ).

Precision: 87.50

Recall: 100.00

F1: 93.33

Score	Text
0.9993	The von Hippel - Lindau ( VHL ) E3 ubiquitin ligase binds ProteinHIF - 1alpha through specific recognition of hydroxylated Pro - 402 or Pro - 564, both of which are modified by the oxygen - dependent HIF prolyl hydroxylases ( PHDs / HPHs ).
0.9910	The von Hippel - Lindau ( VHL ) E3 ubiquitin ligase binds HIF - 1alpha through specific recognition of hydroxylated Pro - 402 or EntityPro - 564 , both of which are modified by the oxygen - dependent HIF prolyl hydroxylases ( PHDs / HPHs ).
0.9771	The von Hippel - Lindau ( VHL ) E3 ubiquitin ligase binds HIF - 1alpha through specific recognition of hydroxylated EntityPro - 402 or Pro - 564, both of which are modified by the oxygen - dependent HIF prolyl hydroxylases ( PHDs / HPHs ).
0.9660	The von Hippel - Lindau ( VHL ) E3 Proteinubiquitin ligase binds HIF - 1alpha through specific recognition of hydroxylated Pro - 402 or Pro - 564, both of which are modified by the oxygen - dependent HIF prolyl hydroxylases ( PHDs / HPHs ).
0.9576	The von Hippel - Lindau ( VHL ) E3 ubiquitin ligase binds HIF - 1alpha through specific recognition of Hydroxylationhydroxylated Pro - 402 or Pro - 564, both of which are modified by the oxygen - dependent HIF prolyl hydroxylases ( PHDs / HPHs ).
0.9187	The von Hippel - Lindau Protein( VHL ) E3 ubiquitin ligase binds HIF - 1alpha through specific recognition of hydroxylated Pro - 402 or Pro - 564, both of which are modified by the oxygen - dependent HIF prolyl hydroxylases ( PHDs / HPHs ).
0.7938	The Proteinvon Hippel - Lindau ( VHL ) E3 ubiquitin ligase binds HIF - 1alpha through specific recognition of hydroxylated Pro - 402 or Pro - 564, both of which are modified by the oxygen - dependent HIF prolyl hydroxylases ( PHDs / HPHs ).
0.5841	The von ProteinHippel - Lindau ( VHL ) E3 ubiquitin ligase binds HIF - 1alpha through specific recognition of hydroxylated Pro - 402 or Pro - 564, both of which are modified by the oxygen - dependent HIF prolyl hydroxylases ( PHDs / HPHs ).

(1226)

Despite the identification of a conserved Leu - X - X - Leu - Ala - Pro motif, the molecular requirement of HIF - 1alpha for PHDs / HPHs binding remains elusive.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Despite the identification of a conserved Leu - X - X - Leu - Ala - Pro motif, the molecular requirement of ProteinHIF - 1alpha for PHDs / HPHs binding remains elusive.

(1227)

Recently, we demonstrated that Leu - 574 of human HIF - 1alpha - - 10 residues downstream of Pro - 564 - - is essential for VHL recognition.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9959	Recently, we demonstrated that Leu - 574 of human ProteinHIF - 1alpha - - 10 residues downstream of Pro - 564 - - is essential for VHL recognition.
0.9952	Recently, we demonstrated that Leu - 574 of human HIF - 1alpha - - 10 residues downstream of Pro - 564 - - is essential for ProteinVHL recognition.

(1228)

We show here that the role of Leu - 574 is to recruit PHD2 / HPH2 for Pro - 564 hydroxylation.

Precision: 100.00

Recall: 75.00

F1: 85.71

Score	Text
0.9905	We show here that the role of Leu - 574 is to recruit ProteinPHD2 / HPH2 for Pro - 564 hydroxylation.
0.9351	We show here that the role of Leu - 574 is to recruit PHD2 / HPH2 for EntityPro - 564 hydroxylation.
0.6596	We show here that the role of Leu - 574 is to recruit PHD2 / HPH2 for Pro - 564 Hydroxylationhydroxylation .
	We show here that the role of Leu - 574 is to recruit PHD2 / HPH2 for Pro - 564 Catalysishydroxylation .

(1229)

An antibody specific for hydroxylated Pro - 564 has been used to determine the hydroxylation status ; mutation or deletion of Leu - 574 results in a significant decrease in the ratio of the hydroxylated HIF - 1alpha to the total amount.

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9870	An antibody specific for hydroxylated Pro - 564 has been used to determine the hydroxylation status ; mutation or deletion of Leu - 574 results in a significant decrease in the ratio of the hydroxylated ProteinHIF - 1alpha to the total amount.
0.9752	An antibody specific for Hydroxylationhydroxylated Pro - 564 has been used to determine the hydroxylation status ; mutation or deletion of Leu - 574 results in a significant decrease in the ratio of the hydroxylated HIF - 1alpha to the total amount.
0.9707	An antibody specific for hydroxylated Pro - 564 has been used to determine the hydroxylation status ; mutation or deletion of Leu - 574 results in a significant decrease in the ratio of the Hydroxylationhydroxylated HIF - 1alpha to the total amount.
0.9686	An antibody specific for hydroxylated EntityPro - 564 has been used to determine the hydroxylation status ; mutation or deletion of Leu - 574 results in a significant decrease in the ratio of the hydroxylated HIF - 1alpha to the total amount.
0.9620	An antibody specific for hydroxylated Pro - 564 has been used to determine the Hydroxylationhydroxylation status ; mutation or deletion of Leu - 574 results in a significant decrease in the ratio of the hydroxylated HIF - 1alpha to the total amount.

(1230)

The nine - residue spacing between Pro - 564 and Leu - 574 is not obligatory for prolyl hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9767	The nine - residue spacing between Pro - 564 and Leu - 574 is not obligatory for prolyl Hydroxylationhydroxylation .
0.9677	The nine - residue spacing between Pro - 564 and Leu - 574 is not obligatory for Entityprolyl hydroxylation.

(1231)

Furthermore, mutation of Leu - 574 disrupts the binding of PHD2 / HPH2, a key prolyl hydroxylase for oxygen - dependent proteolysis of HIF - 1alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Furthermore, mutation of Leu - 574 disrupts the binding of ProteinPHD2 / HPH2 , a key prolyl hydroxylase for oxygen - dependent proteolysis of HIF - 1alpha.
0.9965	Furthermore, mutation of Leu - 574 disrupts the binding of PHD2 / HPH2, a key prolyl hydroxylase for oxygen - dependent proteolysis of ProteinHIF - 1alpha .

(1232)

Hence, our findings indicate that Leu - 574 is essential for recruiting PHD2 / HPH2, thereby providing a molecular basis for modulating HIF - 1alpha activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Hence, our findings indicate that Leu - 574 is essential for recruiting ProteinPHD2 / HPH2 , thereby providing a molecular basis for modulating HIF - 1alpha activity.
0.9995	Hence, our findings indicate that Leu - 574 is essential for recruiting PHD2 / HPH2, thereby providing a molecular basis for modulating ProteinHIF - 1alpha activity.

(1233)

Transferrin microheterogeneity in fetal blood.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9990	ProteinTransferrin microheterogeneity in fetal blood.

(1234)

OBJECTIVES : To investigate the distribution of microheterogeneous subfractions of transferrin in fetal blood and the influence of highly sialylated transferrins on fetal growth.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	OBJECTIVES : To investigate the distribution of microheterogeneous subfractions of Proteintransferrin in fetal blood and the influence of highly sialylated transferrins on fetal growth.
0.9996	OBJECTIVES : To investigate the distribution of microheterogeneous subfractions of transferrin in fetal blood and the influence of highly sialylated Proteintransferrins on fetal growth.

(1235)

STUDY METHOD : Serum transferrin concentrations were determined by a standard turbidimetric assay.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9974	STUDY METHOD : Serum Proteintransferrin concentrations were determined by a standard turbidimetric assay.

(1236)

Microheterogeneous transferrin subfractions were assessed by crossed immunoisoelectric focusing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	Microheterogeneous Proteintransferrin subfractions were assessed by crossed immunoisoelectric focusing.

(1237)

RESULTS : In normal term infants, total serum transferrin concentrations and percent distribution of highly sialylated transferrins ( > or = 5 - sialo - transferrins ) were markedly lower ; the percent distributions of hyposialylated transferrins ( 0 - and 1 - sialo - transferrins ) were apparently higher than those in non - pregnant and pregnant women.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9990	RESULTS : In normal term infants, total serum transferrin concentrations and percent distribution of highly sialylated Proteintransferrins ( > or = 5 - sialo - transferrins ) were markedly lower ; the percent distributions of hyposialylated transferrins ( 0 - and 1 - sialo - transferrins ) were apparently higher than those in non - pregnant and pregnant women.
0.9989	RESULTS : In normal term infants, total serum Proteintransferrin concentrations and percent distribution of highly sialylated transferrins ( > or = 5 - sialo - transferrins ) were markedly lower ; the percent distributions of hyposialylated transferrins ( 0 - and 1 - sialo - transferrins ) were apparently higher than those in non - pregnant and pregnant women.
0.9983	RESULTS : In normal term infants, total serum transferrin concentrations and percent distribution of highly sialylated transferrins ( > or = 5 - sialo - transferrins ) were markedly lower ; the percent distributions of hyposialylated Proteintransferrins ( 0 - and 1 - sialo - transferrins ) were apparently higher than those in non - pregnant and pregnant women.
0.9975	RESULTS : In normal term infants, total serum transferrin concentrations and percent distribution of highly sialylated transferrins ( > or = Protein5 - sialo - transferrins ) were markedly lower ; the percent distributions of hyposialylated transferrins ( 0 - and 1 - sialo - transferrins ) were apparently higher than those in non - pregnant and pregnant women.
0.9891	RESULTS : In normal term infants, total serum transferrin concentrations and percent distribution of highly sialylated transferrins ( > or = 5 - sialo - transferrins ) were markedly lower ; the percent distributions of hyposialylated transferrins ( 0 - and Protein1 - sialo - transferrins ) were apparently higher than those in non - pregnant and pregnant women.

(1238)

There was no significant positive correlation between the serum concentrations of total transferrin or highly sialylated transferrins in infants'blood and birth weights ( r = 0. 187, p = 0. 582 ; r = 0. 374, p = 0. 257, respectively ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	There was no significant positive correlation between the serum concentrations of total Proteintransferrin or highly sialylated transferrins in infants'blood and birth weights ( r = 0. 187, p = 0. 582 ; r = 0. 374, p = 0. 257, respectively ).
0.9993	There was no significant positive correlation between the serum concentrations of total transferrin or highly sialylated Proteintransferrins in infants'blood and birth weights ( r = 0. 187, p = 0. 582 ; r = 0. 374, p = 0. 257, respectively ).

(1239)

CONCLUSION : The transferrin microheterogeneity pattern shifted towards reduced glycosylation and sialylation in addition to a decrease in total transferrin concentration in fetal blood compared to that of non - pregnant and pregnant women.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	CONCLUSION : The Proteintransferrin microheterogeneity pattern shifted towards reduced glycosylation and sialylation in addition to a decrease in total transferrin concentration in fetal blood compared to that of non - pregnant and pregnant women.
0.9990	CONCLUSION : The transferrin microheterogeneity pattern shifted towards reduced glycosylation and sialylation in addition to a decrease in total Proteintransferrin concentration in fetal blood compared to that of non - pregnant and pregnant women.
0.9738	CONCLUSION : The transferrin microheterogeneity pattern shifted towards reduced Glycosylationglycosylation and sialylation in addition to a decrease in total transferrin concentration in fetal blood compared to that of non - pregnant and pregnant women.

(1240)

The concentrations of serum total transferrin and the highly sialylated transferrins in fetal blood, if higher than a certain level, did not seem to have any influence on normal fetal growth.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	The concentrations of serum total Proteintransferrin and the highly sialylated transferrins in fetal blood, if higher than a certain level, did not seem to have any influence on normal fetal growth.
0.9993	The concentrations of serum total transferrin and the highly sialylated Proteintransferrins in fetal blood, if higher than a certain level, did not seem to have any influence on normal fetal growth.

(1241)

Heterochromatin and tri - methylated lysine 20 of histone H4 in animals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9742	Heterochromatin and Methylationtri - methylated lysine 20 of histone H4 in animals.
0.9268	Heterochromatin and tri - methylated lysine 20 of Proteinhistone H4 in animals.
0.8498	Heterochromatin and tri - methylated Entitylysine 20 of histone H4 in animals.

(1242)

Tri - methylated lysine 20 on histone H4 ( Me ( 3 ) K20H4 ) is a marker of constitutive heterochromatin in murine interphase and metaphase cells.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9874	MethylationTri - methylated lysine 20 on histone H4 ( Me ( 3 ) K20H4 ) is a marker of constitutive heterochromatin in murine interphase and metaphase cells.
0.8946	Tri - methylated Entitylysine 20 on histone H4 ( Me ( 3 ) K20H4 ) is a marker of constitutive heterochromatin in murine interphase and metaphase cells.
0.8809	Tri - methylated lysine 20 on Proteinhistone H4 ( Me ( 3 ) K20H4 ) is a marker of constitutive heterochromatin in murine interphase and metaphase cells.
0.6483	Tri - methylated lysine 20 on histone H4 Protein( Me ( 3 ) K20H4 ) is a marker of constitutive heterochromatin in murine interphase and metaphase cells.
0.5268	Tri - methylated Entitylysine 20 on histone H4 ( Me ( 3 ) K20H4 ) is a marker of constitutive heterochromatin in murine interphase and metaphase cells.
0.5082	Tri - methylated lysine 20 on Proteinhistone H4 ( Me ( 3 ) K20H4 ) is a marker of constitutive heterochromatin in murine interphase and metaphase cells.

(1243)

Heterochromatin marked by Me ( 3 ) K20H4 replicates late during S phase of the cell cycle.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9916	Heterochromatin marked by ProteinMe ( 3 ) K20H4 replicates late during S phase of the cell cycle.

(1244)

Serum starvation increases the number of cells that exhibit high levels of Me ( 3 ) K20H4 at constitutive heterochromatin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9752	Serum starvation increases the number of cells that exhibit high levels of ProteinMe ( 3 ) K20H4 at constitutive heterochromatin.

(1245)

Me ( 3 ) K20H4 is also present at the centromeric heterochromatin of most meiotic chromosomes during spermatogenesis and at the pseudoautosomal region, as well as at some telomeres.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9932	ProteinMe ( 3 ) K20H4 is also present at the centromeric heterochromatin of most meiotic chromosomes during spermatogenesis and at the pseudoautosomal region, as well as at some telomeres.

(1246)

It is not present on the XY - body.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1247)

During murine embryogenesis the maternal pronucleus contains Me ( 3 ) K20H4 ; Me ( 3 ) K20H4 is absent from the paternal pronucleus.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9655	During murine embryogenesis the maternal pronucleus contains Me ( 3 ) K20H4 ; ProteinMe ( 3 ) K20H4 is absent from the paternal pronucleus.
0.9477	During murine embryogenesis the maternal pronucleus contains ProteinMe ( 3 ) K20H4 ; Me ( 3 ) K20H4 is absent from the paternal pronucleus.
0.9399	During murine embryogenesis the maternal pronucleus contains ProteinMe ( 3 ) K20H4 ; Me ( 3 ) K20H4 is absent from the paternal pronucleus.

(1248)

On Drosophila polytene chromosomes Me ( 3 ) K20H4 is present in a'punctate pattern'at many chromosomal bands, including the chromocenter.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9936	On Drosophila polytene chromosomes ProteinMe ( 3 ) K20H4 is present in a'punctate pattern'at many chromosomal bands, including the chromocenter.

(1249)

In coccids it is present on the facultatively heterochromatinised paternal chromosome set.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1250)

We also present evidence that Me ( 3 ) K20H4 is dependent upon H3 - specific Suv ( 3 ) 9 histone methyltransferase activity, suggesting that there may be'epigenetic cross - talk'between histones H3 and H4.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9988	We also present evidence that Me ( 3 ) K20H4 is dependent upon H3 - specific Suv ( 3 ) 9 histone methyltransferase activity, suggesting that there may be'epigenetic cross - talk'between histones H3 and ProteinH4 .
0.9911	We also present evidence that ProteinMe ( 3 ) K20H4 is dependent upon H3 - specific Suv ( 3 ) 9 histone methyltransferase activity, suggesting that there may be'epigenetic cross - talk'between histones H3 and H4.
0.9697	We also present evidence that Me ( 3 ) K20H4 is dependent upon H3 - specific Suv ( 3 ) 9 histone methyltransferase activity, suggesting that there may be'epigenetic cross - talk'between Proteinhistones H3 and H4.
0.6694	We also present evidence that Me ( 3 ) K20H4 is dependent upon ProteinH3 - specific Suv ( 3 ) 9 histone methyltransferase activity, suggesting that there may be'epigenetic cross - talk'between histones H3 and H4.
0.6830	We also present evidence that Me ( 3 ) K20H4 is dependent upon H3 - specific ProteinSuv ( 3 ) 9 histone methyltransferase activity, suggesting that there may be'epigenetic cross - talk'between histones H3 and H4.
0.6558	We also present evidence that Me ( 3 ) K20H4 is dependent upon H3 - specific Suv ( 3 ) 9 Proteinhistone methyltransferase activity, suggesting that there may be'epigenetic cross - talk'between histones H3 and H4.

(1251)

The histone modification pattern of active genes revealed through genome - wide chromatin analysis of a higher eukaryote.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	The Proteinhistone modification pattern of active genes revealed through genome - wide chromatin analysis of a higher eukaryote.

(1252)

The covalent modification of nucleosomal histones has emerged as a major determinant of chromatin structure and gene activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9890	The covalent modification of nucleosomal Proteinhistones has emerged as a major determinant of chromatin structure and gene activity.

(1253)

To understand the interplay between various histone modifications, including acetylation and methylation, we performed a genome - wide chromatin structure analysis in a higher eukaryote.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9949	To understand the interplay between various Proteinhistone modifications, including acetylation and methylation, we performed a genome - wide chromatin structure analysis in a higher eukaryote.
0.9871	To understand the interplay between various histone modifications, including Acetylationacetylation and methylation, we performed a genome - wide chromatin structure analysis in a higher eukaryote.
0.8893	To understand the interplay between various histone modifications, including acetylation and Methylationmethylation , we performed a genome - wide chromatin structure analysis in a higher eukaryote.

(1254)

We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.

Precision: 83.33

Recall: 100.00

F1: 90.91

Score	Text
0.9949	We found a binary pattern of Proteinhistone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.9866	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and Lys 79 of ProteinH3 , and inactive genes being hypomethylated and deacetylated at the same residues.
0.9506	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and ProteinH4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.9282	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for ProteinH3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.9132	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and EntityLys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.8731	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at EntityLys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.8616	We found a binary pattern of histone modifications among euchromatic genes, with active genes being Acetylationhyperacetylated for H3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.7554	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and Methylationhypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.6054	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being Methylationhypomethylated and deacetylated at the same residues.
0.3837	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and Deacetylationdeacetylated at the same residues.
0.7354	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and EntityLys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.6472	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at EntityLys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.

(1255)

Furthermore, the degree of modification correlates with the level of transcription, and modifications are largely restricted to transcribed regions, suggesting that their regulation is tightly linked to polymerase activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1256)

Less frequent promoter hypermethylation of DLC - 1 gene in primary breast cancers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9920	Less frequent promoter DNA_methylationhypermethylation of DLC - 1 gene in primary breast cancers.
0.9892	Less frequent promoter hypermethylation of ProteinDLC - 1 gene in primary breast cancers.
0.9601	Less frequent Entitypromoter hypermethylation of DLC - 1 gene in primary breast cancers.

(1257)

Absence or low expression of DLC - 1, a tumor suppressor gene, in breast cancers has been shown recently.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	Absence or low expression of ProteinDLC - 1 , a tumor suppressor gene, in breast cancers has been shown recently.

(1258)

LOH of 8p12 - p22, on which DLC - 1 is located, is frequent in breast cancers, but the correlation between low expression of DLC - 1 and LOH has not been confirmed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	LOH of 8p12 - p22, on which DLC - 1 is located, is frequent in breast cancers, but the correlation between low expression of ProteinDLC - 1 and LOH has not been confirmed.
0.9977	LOH of 8p12 - p22, on which ProteinDLC - 1 is located, is frequent in breast cancers, but the correlation between low expression of DLC - 1 and LOH has not been confirmed.

(1259)

To determine the implication of aberrant methylation, one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the methylation status of DLC - 1 promoter region in breast cancer cell lines and primary breast tumors.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9866	To determine the implication of aberrant methylation, one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the DNA_methylationmethylation status of DLC - 1 promoter region in breast cancer cell lines and primary breast tumors.
0.9374	To determine the implication of aberrant methylation, one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the methylation status of ProteinDLC - 1 promoter region in breast cancer cell lines and primary breast tumors.
0.9167	To determine the implication of aberrant methylation, one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the methylation status of DLC - 1 Entitypromoter region in breast cancer cell lines and primary breast tumors.
0.9389	To determine the implication of aberrant methylation, one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the methylation status of DLC - 1 Entitypromoter region in breast cancer cell lines and primary breast tumors.
0.8262	To determine the implication of aberrant DNA_methylationmethylation , one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the methylation status of DLC - 1 promoter region in breast cancer cell lines and primary breast tumors.
0.5645	To determine the implication of aberrant methylation, one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the methylation status of DLC - 1 promoter Entityregion in breast cancer cell lines and primary breast tumors.

(1260)

The hypermethylation status was examined by MSP and 25 % of cell lines harbored a methylated allele.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9837	The hypermethylation status was examined by MSP and 25 % of cell lines harbored a DNA_methylationmethylated allele.

(1261)

The gene silencing by methylation was also confirmed by the re - expression of DLC - 1 by the 5 - aza - 2'- deoxycytidine treatment in DLC - 1 hypermethylated cell line.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9996	The gene silencing by methylation was also confirmed by the re - expression of ProteinDLC - 1 by the 5 - aza - 2'- deoxycytidine treatment in DLC - 1 hypermethylated cell line.
0.9959	The gene silencing by methylation was also confirmed by the re - expression of DLC - 1 by the 5 - aza - 2'- deoxycytidine treatment in ProteinDLC - 1 hypermethylated cell line.
0.9005	The gene silencing by methylation was also confirmed by the re - expression of DLC - 1 by the 5 - aza - 2'- deoxycytidine treatment in DLC - 1 DNA_methylationhypermethylated cell line.
0.8026	The gene silencing by DNA_methylationmethylation was also confirmed by the re - expression of DLC - 1 by the 5 - aza - 2'- deoxycytidine treatment in DLC - 1 hypermethylated cell line.

(1262)

But the methylation of DLC - 1 gene was less frequently shown in primary breast cancers ( 10 % ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9972	But the methylation of ProteinDLC - 1 gene was less frequently shown in primary breast cancers ( 10 % ).
0.9943	But the DNA_methylationmethylation of DLC - 1 gene was less frequently shown in primary breast cancers ( 10 % ).

(1263)

These data suggest that hypermethylation is responsible for silencing of DLC - 1 gene in a limited portion of breast cancers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9911	These data suggest that hypermethylation is responsible for silencing of ProteinDLC - 1 gene in a limited portion of breast cancers.
0.9896	These data suggest that DNA_methylationhypermethylation is responsible for silencing of DLC - 1 gene in a limited portion of breast cancers.

(1264)

Glycosylation of the ENV spike of primate immunodeficiency viruses and antibody neutralization.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9962	Glycosylation of the ProteinENV spike of primate immunodeficiency viruses and antibody neutralization.
0.9626	GlycosylationGlycosylation of the ENV spike of primate immunodeficiency viruses and antibody neutralization.

(1265)

Neutralizing antibody titers have been correlated with protection following vaccination against many viral pathogens.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1266)

The logical target of protective antibody responses elicited by potential HIV vaccines should be the viral Env spike on the surface of the virion.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	The logical target of protective antibody responses elicited by potential HIV vaccines should be the viral ProteinEnv spike on the surface of the virion.

(1267)

However, the potency and titers of neutralizing antibodies that arise during HIV infection are generally discouragingly low and the antibodies that do arise recognize mainly autologous virus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1268)

This is thought to be a result of a combination of immunodominance of hypervariable regions of the Env protein that can easily escape neutralization, antibody reactivity to gp160 " decoy " protein in cell surface debris or monomeric gp120, conformational constraints within the Env trimer that create unfavorable antibody binding conditions and extensive glycosylation of the exposed regions of Env within the trimer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	This is thought to be a result of a combination of immunodominance of hypervariable regions of the ProteinEnv protein that can easily escape neutralization, antibody reactivity to gp160 " decoy " protein in cell surface debris or monomeric gp120, conformational constraints within the Env trimer that create unfavorable antibody binding conditions and extensive glycosylation of the exposed regions of Env within the trimer.
0.9998	This is thought to be a result of a combination of immunodominance of hypervariable regions of the Env protein that can easily escape neutralization, antibody reactivity to gp160 " decoy " protein in cell surface debris or monomeric gp120, conformational constraints within the ProteinEnv trimer that create unfavorable antibody binding conditions and extensive glycosylation of the exposed regions of Env within the trimer.
0.9990	This is thought to be a result of a combination of immunodominance of hypervariable regions of the Env protein that can easily escape neutralization, antibody reactivity to gp160 " decoy " protein in cell surface debris or monomeric gp120, conformational constraints within the Env trimer that create unfavorable antibody binding conditions and extensive glycosylation of the exposed regions of ProteinEnv within the trimer.
0.9984	This is thought to be a result of a combination of immunodominance of hypervariable regions of the Env protein that can easily escape neutralization, antibody reactivity to Proteingp160 " decoy " protein in cell surface debris or monomeric gp120, conformational constraints within the Env trimer that create unfavorable antibody binding conditions and extensive glycosylation of the exposed regions of Env within the trimer.
0.9979	This is thought to be a result of a combination of immunodominance of hypervariable regions of the Env protein that can easily escape neutralization, antibody reactivity to gp160 " decoy " protein in cell surface debris or monomeric Proteingp120 , conformational constraints within the Env trimer that create unfavorable antibody binding conditions and extensive glycosylation of the exposed regions of Env within the trimer.
0.9382	This is thought to be a result of a combination of immunodominance of hypervariable regions of the Env protein that can easily escape neutralization, antibody reactivity to gp160 " decoy " protein in cell surface debris or monomeric gp120, conformational constraints within the Env trimer that create unfavorable antibody binding conditions and extensive Glycosylationglycosylation of the exposed regions of Env within the trimer.

(1269)

This review will describe current knowledge regarding glycosylation as a mechanism of neutralization resistance and discuss experimental approaches used to overcome this resistance.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1270)

Part of the strategy toward development of an optimally immunogenic Env spike will likely require modification of Env glycosylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Part of the strategy toward development of an optimally immunogenic ProteinEnv spike will likely require modification of Env glycosylation.
0.9977	Part of the strategy toward development of an optimally immunogenic Env spike will likely require modification of ProteinEnv glycosylation.
0.9750	Part of the strategy toward development of an optimally immunogenic Env spike will likely require modification of Env Glycosylationglycosylation .

(1271)

Comparison of methylation - specific polymerase chain reaction ( MSP ) with reverse transcriptase - polymerase chain reaction ( RT - PCR ) in peripheral blood of gastric cancer patients.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9094	Comparison of methylation - specific polymerase chain reaction ( MSP ) with Proteinreverse transcriptase - polymerase chain reaction ( RT - PCR ) in peripheral blood of gastric cancer patients.
0.7326	Comparison of methylation - specific polymerase chain reaction ( MSP ) with reverse Proteintranscriptase - polymerase chain reaction ( RT - PCR ) in peripheral blood of gastric cancer patients.

(1272)

BACKGROUND AND OBJECTIVES : To achieve a complete cure in gastric cancer, primary and recurrent tumors must be detected at an early stage.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1273)

This study was designed to compare carcinoembryonic antigen ( CEA ) - specific reverse transcriptase - polymerase chain reaction ( RT - PCR ) and methylation - specific polymerase chain reaction ( MSP ) for p16, E - cadherin, and retinoic acid receptor beta ( RARbeta ) genes using blood samples from gastric cancer patients.

Precision: 83.33

Recall: 100.00

F1: 90.91

Score	Text
0.9988	This study was designed to compare carcinoembryonic antigen ( CEA ) - specific reverse transcriptase - polymerase chain reaction ( RT - PCR ) and methylation - specific polymerase chain reaction ( MSP ) for p16, ProteinE - cadherin , and retinoic acid receptor beta ( RARbeta ) genes using blood samples from gastric cancer patients.
0.9987	This study was designed to compare carcinoembryonic antigen ( CEA ) - specific reverse transcriptase - polymerase chain reaction ( RT - PCR ) and methylation - specific polymerase chain reaction ( MSP ) for Proteinp16 , E - cadherin, and retinoic acid receptor beta ( RARbeta ) genes using blood samples from gastric cancer patients.
0.9615	This study was designed to compare carcinoembryonic antigen ( CEA ) - specific reverse transcriptase - polymerase chain reaction ( RT - PCR ) and methylation - specific polymerase chain reaction ( MSP ) for p16, E - cadherin, and retinoic acid receptor beta Protein( RARbeta ) genes using blood samples from gastric cancer patients.
0.9401	This study was designed to compare carcinoembryonic antigen ( CEA ) - specific reverse transcriptase - polymerase chain reaction ( RT - PCR ) and methylation - specific polymerase chain reaction ( MSP ) for p16, E - cadherin, and Proteinretinoic acid receptor beta ( RARbeta ) genes using blood samples from gastric cancer patients.
0.9394	This study was designed to compare carcinoembryonic antigen ( CEA ) - specific Proteinreverse transcriptase - polymerase chain reaction ( RT - PCR ) and methylation - specific polymerase chain reaction ( MSP ) for p16, E - cadherin, and retinoic acid receptor beta ( RARbeta ) genes using blood samples from gastric cancer patients.
0.8514	This study was designed to compare carcinoembryonic antigen ( CEA ) - specific reverse Proteintranscriptase - polymerase chain reaction ( RT - PCR ) and methylation - specific polymerase chain reaction ( MSP ) for p16, E - cadherin, and retinoic acid receptor beta ( RARbeta ) genes using blood samples from gastric cancer patients.

(1274)

METHODS : Preoperative blood samples obtained from 41 patients with gastric cancer, including 9 with early - stage disease, and were subjected to RT - PCR and MSP assays.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1275)

RESULTS : Ten of 41 ( 24 % ) patients exhibited a CEA - specific signal by RT - PCR.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1276)

Positive rates were 11, 13, 50, and 50 % in stages I, II, III, and IV, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1277)

A significant association was found between RT - PCR results and stage ( P < 0. 01 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1278)

The MSP assay detected hypermethylation of p16 in 9 patients ( 22 % ), E - cadherin in 9 patients ( 22 % ), and RARbeta in 6 patients ( 15 % ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9973	The MSP assay detected hypermethylation of Proteinp16 in 9 patients ( 22 % ), E - cadherin in 9 patients ( 22 % ), and RARbeta in 6 patients ( 15 % ).
0.9958	The MSP assay detected hypermethylation of p16 in 9 patients ( 22 % ), E - cadherin in 9 patients ( 22 % ), and ProteinRARbeta in 6 patients ( 15 % ).
0.9950	The MSP assay detected hypermethylation of p16 in 9 patients ( 22 % ), ProteinE - cadherin in 9 patients ( 22 % ), and RARbeta in 6 patients ( 15 % ).
0.9851	The MSP assay detected DNA_methylationhypermethylation of p16 in 9 patients ( 22 % ), E - cadherin in 9 patients ( 22 % ), and RARbeta in 6 patients ( 15 % ).

(1279)

Altogether, 18 patients ( 44 % ) showed hypermethylation.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.4971	Altogether, 18 patients ( 44 % ) showed DNA_methylationhypermethylation .

(1280)

The positive rates were 37, 50, 40, and 75 % in stages I, II, III, and IV, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1281)

A significant association was found between aberrant methylation and venous invasion ( P < 0. 05 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1282)

Neither the CEA - specific signal nor hypermethylation was detected in serum from control volunteers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1283)

CONCLUSIONS : The detection rate of MSP was higher than that of RT - PCR in gastric cancer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1284)

Both assays can serve as markers that allow selection of those cases requiring more intensive screening and aggressive postoperative treatment.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1285)

Superoxide dismutase and catalase are required to detect (. - ) NO from both coupled and uncoupled neuronal no synthase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9985	Superoxide dismutase and Proteincatalase are required to detect (. - ) NO from both coupled and uncoupled neuronal no synthase.

(1286)

Despite numerous approaches to measuring nitric oxide ( (. - ) NO ) formation from purified NO synthase ( NOS ), it is still not clear whether (. - ) NO is a direct or indirect product of the NO synthase reaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9846	Despite numerous approaches to measuring nitric oxide ( (. - ) NO ) formation from purified NO synthase Protein( NOS ) , it is still not clear whether (. - ) NO is a direct or indirect product of the NO synthase reaction.
0.9135	Despite numerous approaches to measuring nitric oxide ( (. - ) NO ) formation from purified ProteinNO synthase ( NOS ), it is still not clear whether (. - ) NO is a direct or indirect product of the NO synthase reaction.

(1287)

The direct detection of catalytically formed (. - ) NO is complicated by side reactions with reactive oxide species like H ( 2 ) O ( 2 ) and superoxide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1288)

The aim of the present study was therefore to reinvestigate these reactions both electrochemically and by chemiluminescence detection with particular emphasis on the requirement for cofactors and their interference with (. - ) NO detection.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1289)

Flavins were found to generate large amounts of H ( 2 ) O ( 2 ) and were therefore excluded from subsequent incubations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1290)

Under conditions of both coupled and uncoupled catalysis, SOD was absolutely required to detect (. - ) NO from NOS.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1291)

H ( 2 ) O ( 2 ) formation took place also in the presence of SOD and gave a smaller yet significant interfering signal.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1292)

Similar data were obtained when the proposed intermediate N ( omega ) - hydroxy - l - arginine was utilized as substrate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1293)

In conclusion, standard Clark - type ( ) NO electrodes are cross - sensitive to H ( 2 ) O ( 2 ) and therefore both SOD and catalase are absolutely required to specifically detect (. - ) NO from NOS.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	In conclusion, standard Clark - type ( ) NO electrodes are cross - sensitive to H ( 2 ) O ( 2 ) and therefore both SOD and Proteincatalase are absolutely required to specifically detect (. - ) NO from NOS.

(1294)

Widespread activation of antibiotic biosynthesis by S - adenosylmethionine in streptomycetes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1295)

The effect of S - adenosylmethionine ( SAM ) on the production of various antibiotics was investigated to determine whether SAM - dependent methylation is required in biosynthetic pathways of antibiotics.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1296)

Pristinamycin II ( B ) and granaticin do not require SAM - dependent methylation in their biosynthesis pathways, and production of these two antibiotics was increased about 2 - fold when a low concentration ( 50 and 10 microM, respectively ) of SAM was treated ; in contrast, oleandomycin and avermectin B1a require SAM as a methyl donor in their biosynthesis, and production of these two antibiotics was increased 5 - fold and 6 - fold, depending on the SAM concentration within a certain range.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1297)

We also found that the transcription of a pathway - specific regulator, gra - ORF9, was activated by exogenous SAM treatment.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	We also found that the transcription of a pathway - specific regulator, Proteingra - ORF9 , was activated by exogenous SAM treatment.

(1298)

Production of oleandomycin and avermectin B1a was decreased by using a methyltransferase inhibitor, sinefungin, but the production levels of these antibiotics were restored to the control level by simultaneously adding SAM and sinefungin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1299)

Interestingly, we have found a similar stimulatory effect of S - adenosylhomocysteine ( SAH ), the methylation product of SAM, on antibiotic production in the four strains.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1300)

Our results clearly demonstrate the widespread activation of antibiotic production using SAM in streptomycetes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1301)

A phosphatidylserine - binding site in the cytosolic fragment of Clostridium sordellii lethal toxin facilitates glucosylation of membrane - bound Rac and is required for cytotoxicity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1302)

Large clostridial toxins glucosylate some small G proteins on a threonine residue, thereby preventing their interactions with effector molecules and regulators.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1303)

We show that the glucosyltransferase domain of lethal toxin from Clostridium sordellii ( LT ( cyt ) ; amino acids 1 - 546 ), which is released into the cytosol during cell infection, binds preferentially to liposomes containing phosphatidylserine as compared with other anionic lipids.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9701	We show that the glucosyltransferase domain of lethal toxin from Clostridium sordellii Protein( LT ( cyt ) ; amino acids 1 - 546 ), which is released into the cytosol during cell infection, binds preferentially to liposomes containing phosphatidylserine as compared with other anionic lipids.

(1304)

The binding of LT ( cyt ) to phosphatidylserine increases by two orders of magnitude the rate of glucosylation of liposome - bound geranyl - geranylated Rac - GDP.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9985	The binding of ProteinLT ( cyt ) to phosphatidylserine increases by two orders of magnitude the rate of glucosylation of liposome - bound geranyl - geranylated Rac - GDP.

(1305)

Limited proteolysis and deletion studies show that the binding site for phosphatidylserine lies within the first 18 N - terminal residues of LT ( cyt ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9940	Limited proteolysis and deletion studies show that the binding site for phosphatidylserine lies within the first 18 N - terminal residues of ProteinLT ( cyt ) .

(1306)

Deletion of these residues abolishes the effect of phosphatidylserine on the activity of LT ( cyt ) on liposome - bound geranyl - geranylated Rac - GDP and prevents the morphological effects induced by LT ( cyt ) microinjection into various cells, but it does not affect the intrinsic activity of LT ( cyt ) on non - geranyl - geranylated Rac - GDP in solution.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9985	Deletion of these residues abolishes the effect of phosphatidylserine on the activity of ProteinLT ( cyt ) on liposome - bound geranyl - geranylated Rac - GDP and prevents the morphological effects induced by LT ( cyt ) microinjection into various cells, but it does not affect the intrinsic activity of LT ( cyt ) on non - geranyl - geranylated Rac - GDP in solution.
0.9978	Deletion of these residues abolishes the effect of phosphatidylserine on the activity of LT ( cyt ) on liposome - bound geranyl - geranylated Rac - GDP and prevents the morphological effects induced by ProteinLT ( cyt ) microinjection into various cells, but it does not affect the intrinsic activity of LT ( cyt ) on non - geranyl - geranylated Rac - GDP in solution.
0.9972	Deletion of these residues abolishes the effect of phosphatidylserine on the activity of LT ( cyt ) on liposome - bound geranyl - geranylated Rac - GDP and prevents the morphological effects induced by LT ( cyt ) microinjection into various cells, but it does not affect the intrinsic activity of ProteinLT ( cyt ) on non - geranyl - geranylated Rac - GDP in solution.

(1307)

We conclude that the avidity of LT ( cyt ) for phosphatidylserine facilitates its targeting to the cytosolic leaflet of cell membranes and, notably, the plasma membrane, where this anionic lipid is abundant and where several targets of lethal toxin reside.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9984	We conclude that the avidity of ProteinLT ( cyt ) for phosphatidylserine facilitates its targeting to the cytosolic leaflet of cell membranes and, notably, the plasma membrane, where this anionic lipid is abundant and where several targets of lethal toxin reside.

(1308)

Arginine residues as stabilizing elements in proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1309)

Site - specific substitutions of arginine for lysine in the thermostable D - xylose isomerase ( XI ) from Actinoplanes missouriensis are shown to impart significant heat stability enhancement in the presence of sugar substrates most probably by interfering with nonenzymatic glycation.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9663	Site - specific substitutions of arginine for lysine in the thermostable D - xylose isomerase Protein( XI ) from Actinoplanes missouriensis are shown to impart significant heat stability enhancement in the presence of sugar substrates most probably by interfering with nonenzymatic glycation.
0.9347	Site - specific substitutions of arginine for lysine in the thermostable ProteinD - xylose isomerase ( XI ) from Actinoplanes missouriensis are shown to impart significant heat stability enhancement in the presence of sugar substrates most probably by interfering with nonenzymatic glycation.
0.5951	Site - specific substitutions of arginine for lysine in the thermostable ProteinD - xylose isomerase ( XI ) from Actinoplanes missouriensis are shown to impart significant heat stability enhancement in the presence of sugar substrates most probably by interfering with nonenzymatic glycation.

(1310)

The same substitutions are also found to increase heat stability in the absence of any sugar derivatives, where a mechanism based on prevention of glycation can no longer be invoked.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1311)

This rather conservative substitution is moreover shown to improve thermostability in two other structurally unrelated proteins, human copper, zinc - superoxide dismutase ( CuZnSOD ) and D - glyceraldehyde - 3 - phosphate dehydrogenase ( GAPDH ) from Bacillus subtilis.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9819	This rather conservative substitution is moreover shown to improve thermostability in two other structurally unrelated proteins, human copper, zinc - superoxide dismutase ( CuZnSOD ) and D - glyceraldehyde - 3 - phosphate dehydrogenase Protein( GAPDH ) from Bacillus subtilis.
0.9721	This rather conservative substitution is moreover shown to improve thermostability in two other structurally unrelated proteins, human copper, zinc - superoxide dismutase Protein( CuZnSOD ) and D - glyceraldehyde - 3 - phosphate dehydrogenase ( GAPDH ) from Bacillus subtilis.
0.9103	This rather conservative substitution is moreover shown to improve thermostability in two other structurally unrelated proteins, human copper, zinc - superoxide dismutase ( CuZnSOD ) and ProteinD - glyceraldehyde - 3 - phosphate dehydrogenase ( GAPDH ) from Bacillus subtilis.
0.8498	This rather conservative substitution is moreover shown to improve thermostability in two other structurally unrelated proteins, human Proteincopper, zinc - superoxide dismutase ( CuZnSOD ) and D - glyceraldehyde - 3 - phosphate dehydrogenase ( GAPDH ) from Bacillus subtilis.
0.8003	This rather conservative substitution is moreover shown to improve thermostability in two other structurally unrelated proteins, human copper, Proteinzinc - superoxide dismutase ( CuZnSOD ) and D - glyceraldehyde - 3 - phosphate dehydrogenase ( GAPDH ) from Bacillus subtilis.
0.7742	This rather conservative substitution is moreover shown to improve thermostability in two other structurally unrelated proteins, human copper Protein, zinc - superoxide dismutase ( CuZnSOD ) and D - glyceraldehyde - 3 - phosphate dehydrogenase ( GAPDH ) from Bacillus subtilis.

(1312)

The stabilizing effect of Lys - - - - Arg substitutions is rationalized on the basis of a detailed analysis of the crystal structures of wild - type XI and of engineered variants with Lys - - - - Arg substitution at four distinct locations, residues 253, 309, 319, and 323.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9960	The stabilizing effect of Lys - - - - Arg substitutions is rationalized on the basis of a detailed analysis of the crystal structures of wild - type ProteinXI and of engineered variants with Lys - - - - Arg substitution at four distinct locations, residues 253, 309, 319, and 323.

(1313)

Molecular model building analysis of the structures of wild - type and mutant CuZnSOD ( K9R ) and GAPDH ( G281K and G281R ) is used to explain the observed stability enhancement in these proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9974	Molecular model building analysis of the structures of wild - type and mutant CuZnSOD ( K9R ) and ProteinGAPDH ( G281K and G281R ) is used to explain the observed stability enhancement in these proteins.
0.9869	Molecular model building analysis of the structures of wild - type and mutant ProteinCuZnSOD ( K9R ) and GAPDH ( G281K and G281R ) is used to explain the observed stability enhancement in these proteins.

(1314)

In addition to demonstrating that even thermostable proteins can lend themselves to further stability improvement, our findings provide direct evidence that arginine residues are important stabilizing elements in proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1315)

Moreover, the stabilizing role of electrostatic interactions, particularly between subunits in oligomeric proteins, is documented.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1316)

Human SWI / SNF - associated PRMT5 methylates histone H3 arginine 8 and negatively regulates expression of ST7 and NM23 tumor suppressor genes.

Precision: 100.00

Recall: 85.71

F1: 92.31

Score	Text
0.9929	Human SWI / SNF - associated PRMT5 methylates histone H3 arginine 8 and negatively regulates expression of ST7 and ProteinNM23 tumor suppressor genes.
0.9891	Human SWI / SNF - associated ProteinPRMT5 methylates histone H3 arginine 8 and negatively regulates expression of ST7 and NM23 tumor suppressor genes.
0.9872	Human SWI / SNF - associated PRMT5 methylates histone H3 arginine 8 and negatively regulates expression of ProteinST7 and NM23 tumor suppressor genes.
0.5813	Human SWI / SNF - associated PRMT5 methylates Proteinhistone H3 arginine 8 and negatively regulates expression of ST7 and NM23 tumor suppressor genes.
0.5519	Human SWI / SNF - associated PRMT5 methylates histone H3 Entityarginine 8 and negatively regulates expression of ST7 and NM23 tumor suppressor genes.
0.5334	Human SWI / SNF - associated PRMT5 Methylationmethylates histone H3 arginine 8 and negatively regulates expression of ST7 and NM23 tumor suppressor genes.
	Human SWI / SNF - associated PRMT5 Catalysismethylates histone H3 arginine 8 and negatively regulates expression of ST7 and NM23 tumor suppressor genes.

(1317)

Protein arginine methyltransferases ( PRMTs ) have been implicated in transcriptional activation and repression, but their role in controlling cell growth and proliferation remains obscure.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1318)

We have recently shown that PRMT5 can interact with flag - tagged BRG1 - and hBRM - based hSWI / SNF chromatin remodelers and that both complexes can specifically methylate histones H3 and H4.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9994	We have recently shown that ProteinPRMT5 can interact with flag - tagged BRG1 - and hBRM - based hSWI / SNF chromatin remodelers and that both complexes can specifically methylate histones H3 and H4.
0.9992	We have recently shown that PRMT5 can interact with flag - tagged ProteinBRG1 - and hBRM - based hSWI / SNF chromatin remodelers and that both complexes can specifically methylate histones H3 and H4.
0.9968	We have recently shown that PRMT5 can interact with flag - tagged BRG1 - and ProteinhBRM - based hSWI / SNF chromatin remodelers and that both complexes can specifically methylate histones H3 and H4.
0.9908	We have recently shown that PRMT5 can interact with flag - tagged BRG1 - and hBRM - based hSWI / SNF chromatin remodelers and that both complexes can specifically methylate histones H3 and ProteinH4 .
0.9304	We have recently shown that PRMT5 can interact with flag - tagged BRG1 - and hBRM - based hSWI / SNF chromatin remodelers and that both complexes can specifically methylate Proteinhistones H3 and H4.
0.8629	We have recently shown that PRMT5 can interact with flag - tagged BRG1 - and hBRM - based hSWI / SNF chromatin remodelers and that both complexes can specifically Methylationmethylate histones H3 and H4.

(1319)

Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 arginine 8 and H4 arginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated PRMT5.

Precision: 81.82

Recall: 90.00

F1: 85.71

Score	Text
0.9991	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 arginine 8 and H4 arginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated ProteinPRMT5 .
0.9989	Here we report that ProteinPRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 arginine 8 and H4 arginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated PRMT5.
0.9927	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and ProteinH4 N - terminal tails, and show that H3 arginine 8 and H4 arginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated PRMT5.
0.9897	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate ProteinH3 and H4 N - terminal tails, and show that H3 arginine 8 and H4 arginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated PRMT5.
0.9599	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that ProteinH3 arginine 8 and H4 arginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated PRMT5.
0.9479	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 arginine 8 and ProteinH4 arginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated PRMT5.
0.9199	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 Entityarginine 8 and H4 arginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated PRMT5.
0.8236	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 arginine 8 and H4 Entityarginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated PRMT5.
0.6727	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 arginine 8 and H4 arginine 3 are preferred sites of Methylationmethylation by recombinant and hSWI / SNF - associated PRMT5.
0.6351	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 Entityarginine 8 and H4 arginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated PRMT5.
0.5712	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can Catalysismethylate H3 and H4 N - terminal tails, and show that H3 arginine 8 and H4 arginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated PRMT5.
	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 arginine 8 and H4 arginine 3 are preferred sites of Catalysismethylation by recombinant and hSWI / SNF - associated PRMT5.

(1320)

To elucidate the role played by PRMT5 in gene regulation, we have established a PRMT5 antisense cell line and determined by microarray analysis that more genes are derepressed when PRMT5 levels are reduced.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9985	To elucidate the role played by ProteinPRMT5 in gene regulation, we have established a PRMT5 antisense cell line and determined by microarray analysis that more genes are derepressed when PRMT5 levels are reduced.
0.9969	To elucidate the role played by PRMT5 in gene regulation, we have established a ProteinPRMT5 antisense cell line and determined by microarray analysis that more genes are derepressed when PRMT5 levels are reduced.
0.9962	To elucidate the role played by PRMT5 in gene regulation, we have established a PRMT5 antisense cell line and determined by microarray analysis that more genes are derepressed when ProteinPRMT5 levels are reduced.

(1321)

Among the affected genes, we show that suppressor of tumorigenicity 7 ( ST7 ) and nonmetastatic 23 ( NM23 ) are direct targets of PRMT5 - containing BRG1 and hBRM complexes.

Precision: 70.00

Recall: 100.00

F1: 82.35

Score	Text
0.9962	Among the affected genes, we show that suppressor of tumorigenicity 7 ( ST7 ) and nonmetastatic 23 ( NM23 ) are direct targets of PRMT5 - containing BRG1 and ProteinhBRM complexes.
0.9935	Among the affected genes, we show that suppressor of tumorigenicity 7 ( ST7 ) and nonmetastatic 23 ( NM23 ) are direct targets of PRMT5 - containing ProteinBRG1 and hBRM complexes.
0.9745	Among the affected genes, we show that suppressor of tumorigenicity 7 ( ST7 ) and nonmetastatic 23 Protein( NM23 ) are direct targets of PRMT5 - containing BRG1 and hBRM complexes.
0.9604	Among the affected genes, we show that suppressor of tumorigenicity 7 ( ST7 ) and nonmetastatic 23 ( NM23 ) are direct targets of ProteinPRMT5 - containing BRG1 and hBRM complexes.
0.9591	Among the affected genes, we show that suppressor of tumorigenicity 7 Protein( ST7 ) and nonmetastatic 23 ( NM23 ) are direct targets of PRMT5 - containing BRG1 and hBRM complexes.
0.9141	Among the affected genes, we show that suppressor of tumorigenicity 7 ( ST7 ) and Proteinnonmetastatic 23 ( NM23 ) are direct targets of PRMT5 - containing BRG1 and hBRM complexes.
0.7518	Among the affected genes, we show that Proteinsuppressor of tumorigenicity 7 ( ST7 ) and nonmetastatic 23 ( NM23 ) are direct targets of PRMT5 - containing BRG1 and hBRM complexes.
0.9664	Among the affected genes, we show that suppressor of tumorigenicity 7 ( ST7 ) and nonmetastatic 23 ( NM23 ) are direct targets of ProteinPRMT5 - containing BRG1 and hBRM complexes.
0.5466	Among the affected genes, we show that suppressor of Proteintumorigenicity 7 ( ST7 ) and nonmetastatic 23 ( NM23 ) are direct targets of PRMT5 - containing BRG1 and hBRM complexes.
0.5392	Among the affected genes, we show that suppressor Proteinof tumorigenicity 7 ( ST7 ) and nonmetastatic 23 ( NM23 ) are direct targets of PRMT5 - containing BRG1 and hBRM complexes.

(1322)

Furthermore, we demonstrate that expression of ST7 and NM23 is reduced in a cell line that overexpresses PRMT5 and that this decrease in expression correlates with H3R8 methylation, H3K9 deacetylation, and increased transformation of NIH 3T3 cells.

Precision: 100.00

Recall: 77.78

F1: 87.50

Score	Text
0.9998	Furthermore, we demonstrate that expression of ST7 and NM23 is reduced in a cell line that overexpresses ProteinPRMT5 and that this decrease in expression correlates with H3R8 methylation, H3K9 deacetylation, and increased transformation of NIH 3T3 cells.
0.9988	Furthermore, we demonstrate that expression of ProteinST7 and NM23 is reduced in a cell line that overexpresses PRMT5 and that this decrease in expression correlates with H3R8 methylation, H3K9 deacetylation, and increased transformation of NIH 3T3 cells.
0.9984	Furthermore, we demonstrate that expression of ST7 and ProteinNM23 is reduced in a cell line that overexpresses PRMT5 and that this decrease in expression correlates with H3R8 methylation, H3K9 deacetylation, and increased transformation of NIH 3T3 cells.
0.7305	Furthermore, we demonstrate that expression of ST7 and NM23 is reduced in a cell line that overexpresses PRMT5 and that this decrease in expression correlates with H3R8 Methylationmethylation , H3K9 deacetylation, and increased transformation of NIH 3T3 cells.
0.6449	Furthermore, we demonstrate that expression of ST7 and NM23 is reduced in a cell line that overexpresses PRMT5 and that this decrease in expression correlates with H3R8 methylation, EntityH3K9 deacetylation, and increased transformation of NIH 3T3 cells.
0.5277	Furthermore, we demonstrate that expression of ST7 and NM23 is reduced in a cell line that overexpresses PRMT5 and that this decrease in expression correlates with H3R8 methylation, H3K9 Deacetylationdeacetylation , and increased transformation of NIH 3T3 cells.
0.5087	Furthermore, we demonstrate that expression of ST7 and NM23 is reduced in a cell line that overexpresses PRMT5 and that this decrease in expression correlates with EntityH3R8 methylation, H3K9 deacetylation, and increased transformation of NIH 3T3 cells.
	Furthermore, we demonstrate that expression of ST7 and NM23 is reduced in a cell line that overexpresses PRMT5 and that this decrease in expression correlates with ProteinH3R8 methylation, H3K9 deacetylation, and increased transformation of NIH 3T3 cells.
	Furthermore, we demonstrate that expression of ST7 and NM23 is reduced in a cell line that overexpresses PRMT5 and that this decrease in expression correlates with H3R8 methylation, ProteinH3K9 deacetylation, and increased transformation of NIH 3T3 cells.

(1323)

These findings suggest that the BRG1 - and hBRM - associated PRMT5 regulates cell growth and proliferation by controlling expression of genes involved in tumor suppression.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9970	These findings suggest that the ProteinBRG1 - and hBRM - associated PRMT5 regulates cell growth and proliferation by controlling expression of genes involved in tumor suppression.
0.9871	These findings suggest that the BRG1 - and ProteinhBRM - associated PRMT5 regulates cell growth and proliferation by controlling expression of genes involved in tumor suppression.
0.6126	These findings suggest that the BRG1 - and hBRM - associated ProteinPRMT5 regulates cell growth and proliferation by controlling expression of genes involved in tumor suppression.
0.8841	These findings suggest that the BRG1 - and ProteinhBRM - associated PRMT5 regulates cell growth and proliferation by controlling expression of genes involved in tumor suppression.

(1324)

Sex - and tissue - specific expression of maintenance and de novo DNA methyltransferases upon low dose X - irradiation in mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1325)

DNA methylation is crucial for normal development, proliferation, and proper maintenance of genome stability for a given organism.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1326)

A variety of DNA damaging agents that are known to affect genome stability were also shown to alter DNA methylation patterns.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1327)

We have recently pioneered the studies in the area of the radiation effects on DNA methylation, and found that radiation exposure led to substantial dose - dependent and tissue - specific DNA hypomethylation, which was much more pronounced in spleen and liver of female animals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1328)

The exact mechanisms of radiation - induced DNA hypomethylation are still to be uncovered.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1329)

We have previously shown that one of those mechanisms may potentially be DNA repair related.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1330)

Another possible mechanism may be linked to changes in the expression of DNA methyltransferases ( DNMTs ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1331)

In the current study, we examined the radiation - induced changes in expression of maintenance DNMT1, and de novo methyltransferases DNMT3a and DNMT3b in spleen and liver of irradiated animals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	In the current study, we examined the radiation - induced changes in expression of maintenance ProteinDNMT1 , and de novo methyltransferases DNMT3a and DNMT3b in spleen and liver of irradiated animals.
0.9998	In the current study, we examined the radiation - induced changes in expression of maintenance DNMT1, and de novo methyltransferases ProteinDNMT3a and DNMT3b in spleen and liver of irradiated animals.
0.9998	In the current study, we examined the radiation - induced changes in expression of maintenance DNMT1, and de novo methyltransferases DNMT3a and ProteinDNMT3b in spleen and liver of irradiated animals.

(1332)

This was paralleled by the studies of acute and chronic IR - induced methylation changes in spleen and liver of intact animals, as well as in animals with altered sex hormone status.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1333)

Here we report that radiation - induced DNA methylation changes correlated with radiation - induced alterations in expression of DNA methyltransferases.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1334)

We present the data on tissue - specificity in radiation - induced expression of DNA methyltransferases, and prove that changes in the expression of de novo methyltransferases DNMT3a and DNMT3b are the most important in radiation - induced DNA methylation alterations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	We present the data on tissue - specificity in radiation - induced expression of DNA methyltransferases, and prove that changes in the expression of de novo methyltransferases DNMT3a and ProteinDNMT3b are the most important in radiation - induced DNA methylation alterations.
0.9998	We present the data on tissue - specificity in radiation - induced expression of DNA methyltransferases, and prove that changes in the expression of de novo methyltransferases ProteinDNMT3a and DNMT3b are the most important in radiation - induced DNA methylation alterations.

(1335)

We also discuss the role of sex hormones, especially estrogen, in the generation of the sex - specific radiation - induced methylation changes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1336)

Characterization of a novel amylolytic enzyme encoded by a gene from a soil - derived metagenomic library.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1337)

It has been estimated that less than 1 % of the microorganisms in nature can be cultivated by conventional techniques.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1338)

Thus, the classical approach of isolating enzymes from pure cultures allows the analysis of only a subset of the total naturally occurring microbiota in environmental samples enriched in microorganisms.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1339)

To isolate useful microbial enzymes from uncultured soil microorganisms, a metagenome was isolated from soil samples, and a metagenomic library was constructed by using the pUC19 vector.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.9290	To isolate useful microbial enzymes from uncultured soil microorganisms, a metagenome was isolated from soil samples, and a metagenomic library was constructed by using the ProteinpUC19 vector.

(1340)

The library was screened for amylase activity, and one clone from among approximately 30, 000 recombinant Escherichia coli clones showed amylase activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1341)

Sequencing of the clone revealed a novel amylolytic enzyme expressed from a novel gene.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1342)

The putative amylase gene ( amyM ) was overexpressed and purified for characterization.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9974	The putative amylase gene Protein( amyM ) was overexpressed and purified for characterization.

(1343)

Optimal conditions for the enzyme activity of the AmyM protein were 42 degrees C and pH 9. 0 ; Ca2 + stabilized the activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	Optimal conditions for the enzyme activity of the ProteinAmyM protein were 42 degrees C and pH 9. 0 ; Ca2 + stabilized the activity.

(1344)

The amylase hydrolyzed soluble starch and cyclodextrins to produce high levels of maltose and hydrolyzed pullulan to panose.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1345)

The enzyme showed a high transglycosylation activity, making alpha - ( 1, 4 ) linkages exclusively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1346)

The hydrolysis and transglycosylation properties of AmyM suggest that it has novel characteristics and can be regarded as an intermediate type of maltogenic amylase, alpha - amylase, and 4 - alpha - glucanotransferase.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9979	The hydrolysis and transglycosylation properties of ProteinAmyM suggest that it has novel characteristics and can be regarded as an intermediate type of maltogenic amylase, alpha - amylase, and 4 - alpha - glucanotransferase.
0.9341	The hydrolysis and transglycosylation properties of AmyM suggest that it has novel characteristics and can be regarded as an intermediate type of maltogenic amylase, alpha - amylase, and Protein4 - alpha - glucanotransferase .
0.4699	The hydrolysis and Glycosylationtransglycosylation properties of AmyM suggest that it has novel characteristics and can be regarded as an intermediate type of maltogenic amylase, alpha - amylase, and 4 - alpha - glucanotransferase.

(1347)

4 - Hydroxyphenylpyruvate dioxygenase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.8859	Protein4 - Hydroxyphenylpyruvate dioxygenase .

(1348)

4 - Hydroxyphenylpyruvate dioxygenase ( HPPD ) is an Fe ( II ) - dependent, non - heme oxygenase that catalyzes the conversion of 4 - hydroxyphenylpyruvate to homogentisate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9759	4 - Hydroxyphenylpyruvate dioxygenase Protein( HPPD ) is an Fe ( II ) - dependent, non - heme oxygenase that catalyzes the conversion of 4 - hydroxyphenylpyruvate to homogentisate.
0.8469	Protein4 - Hydroxyphenylpyruvate dioxygenase ( HPPD ) is an Fe ( II ) - dependent, non - heme oxygenase that catalyzes the conversion of 4 - hydroxyphenylpyruvate to homogentisate.

(1349)

This reaction involves decarboxylation, substituent migration and aromatic oxygenation in a single catalytic cycle.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1350)

HPPD is a member of the alpha - keto acid dependent oxygenases that typically require an alpha - keto acid ( almost exclusively alpha - ketoglutarate ) and molecular oxygen to either oxygenate or oxidize a third molecule.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	ProteinHPPD is a member of the alpha - keto acid dependent oxygenases that typically require an alpha - keto acid ( almost exclusively alpha - ketoglutarate ) and molecular oxygen to either oxygenate or oxidize a third molecule.

(1351)

As an exception in this class of enzymes HPPD has only two substrates, does not use alpha - ketoglutarate, and incorporates both atoms of dioxygen into the aromatic product, homogentisate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	As an exception in this class of enzymes ProteinHPPD has only two substrates, does not use alpha - ketoglutarate, and incorporates both atoms of dioxygen into the aromatic product, homogentisate.

(1352)

The tertiary structure of the enzyme would suggest that its mechanism converged with that of other alpha - keto acid enzymes from an extradiol dioxygenase progenitor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1353)

The transformation catalyzed by HPPD has both agricultural and therapeutic significance.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	The transformation catalyzed by ProteinHPPD has both agricultural and therapeutic significance.

(1354)

HPPD catalyzes the second step in the pathway for the catabolism of tyrosine, that is common to essentially all aerobic forms of life.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	ProteinHPPD catalyzes the second step in the pathway for the catabolism of tyrosine, that is common to essentially all aerobic forms of life.

(1355)

In plants this pathway has an anabolic branch from homogentisate that forms essential isoprenoid redox cofactors such as plastoquinone and tocopherol.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1356)

Naturally occurring multi - ketone molecules act as allelopathic agents by inhibiting HPPD and preventing the production of homogentisate and hence required redox cofactors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9989	Naturally occurring multi - ketone molecules act as allelopathic agents by inhibiting ProteinHPPD and preventing the production of homogentisate and hence required redox cofactors.

(1357)

This has been the basis for the development of a range of very effective herbicides that are currently used commercially.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1358)

In humans, deficiencies of specific enzymes of the tyrosine catabolism pathway give rise to a number of severe metabolic disorders.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1359)

Interestingly, HPPD inhibitor / herbicide molecules act also as therapeutic agents for a number of debilitating and lethal inborn defects in tyrosine catabolism by preventing the accumulation of toxic metabolites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9983	Interestingly, ProteinHPPD inhibitor / herbicide molecules act also as therapeutic agents for a number of debilitating and lethal inborn defects in tyrosine catabolism by preventing the accumulation of toxic metabolites.

(1360)

Distinct dynamics and distribution of histone methyl - lysine derivatives in mouse development.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9881	Distinct dynamics and distribution of Proteinhistone methyl - lysine derivatives in mouse development.

(1361)

Histone methylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and lysine residues on histones H3 and H4.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Text
0.9729	Histone methylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and lysine residues on histones H3 and ProteinH4 .
0.9491	Histone methylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and lysine residues on Proteinhistones H3 and H4.
0.9373	Histone Methylationmethylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and lysine residues on histones H3 and H4.
0.9284	ProteinHistone methylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and lysine residues on histones H3 and H4.
0.9095	Histone methylation acts as an epigenetic regulator of chromatin activity through the modification of Entityarginine and lysine residues on histones H3 and H4.
0.8980	Histone methylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and Entitylysine residues on histones H3 and H4.
0.7104	Histone methylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and Entitylysine residues on histones H3 and H4.
0.5154	Histone methylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and lysine residues on Proteinhistones H3 and H4.

(1362)

In the case of lysine, this includes the formation of mono -, di -, or trimethyl groups, each of which is presumed to represent a distinct functional state at the cellular level.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1363)

To examine the potential developmental roles of these modifications, we determined the global patterns of lysine methylation involving K9 on histone H3 and K20 on histone H4 in midgestation mouse embryos.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9925	To examine the potential developmental roles of these modifications, we determined the global patterns of lysine methylation involving K9 on histone H3 and EntityK20 on histone H4 in midgestation mouse embryos.
0.9920	To examine the potential developmental roles of these modifications, we determined the global patterns of lysine methylation involving EntityK9 on histone H3 and K20 on histone H4 in midgestation mouse embryos.
0.9461	To examine the potential developmental roles of these modifications, we determined the global patterns of lysine methylation involving K9 on Proteinhistone H3 and K20 on histone H4 in midgestation mouse embryos.
0.9343	To examine the potential developmental roles of these modifications, we determined the global patterns of lysine methylation involving K9 on histone H3 and K20 on Proteinhistone H4 in midgestation mouse embryos.
0.9261	To examine the potential developmental roles of these modifications, we determined the global patterns of lysine Methylationmethylation involving K9 on histone H3 and K20 on histone H4 in midgestation mouse embryos.

(1364)

For each lysine target site, we observed distinct subnuclear distributions of the mono - and trimethyl versions in 10T1 / 2 cells that were conserved within primary cultures and within the 3D - tissue architecture of the embryo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1365)

Interestingly, three of these modifications, histone H3 trimethyl K9, histone H4 monomethyl K20, and histone H4 trimethyl K20 exhibited marked differences in their distribution within the neuroepithelium.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9743	Interestingly, three of these modifications, histone H3 trimethyl EntityK9 , histone H4 monomethyl K20, and histone H4 trimethyl K20 exhibited marked differences in their distribution within the neuroepithelium.
0.9684	Interestingly, three of these modifications, histone H3 trimethyl K9, histone H4 monomethyl K20, and histone H4 trimethyl EntityK20 exhibited marked differences in their distribution within the neuroepithelium.
0.9539	Interestingly, three of these modifications, histone H3 trimethyl K9, histone H4 monomethyl EntityK20 , and histone H4 trimethyl K20 exhibited marked differences in their distribution within the neuroepithelium.
0.9449	Interestingly, three of these modifications, Proteinhistone H3 trimethyl K9, histone H4 monomethyl K20, and histone H4 trimethyl K20 exhibited marked differences in their distribution within the neuroepithelium.
0.9394	Interestingly, three of these modifications, histone H3 trimethyl K9, Proteinhistone H4 monomethyl K20, and histone H4 trimethyl K20 exhibited marked differences in their distribution within the neuroepithelium.
0.9185	Interestingly, three of these modifications, histone H3 trimethyl K9, histone H4 monomethyl K20, and Proteinhistone H4 trimethyl K20 exhibited marked differences in their distribution within the neuroepithelium.
0.8652	Interestingly, three of these Methylationmodifications , histone H3 trimethyl K9, histone H4 monomethyl K20, and histone H4 trimethyl K20 exhibited marked differences in their distribution within the neuroepithelium.

(1366)

Specifically, both histone H3 trimethyl K9 and H4 monomethyl K20 were elevated in proliferating cells of the neural tube, which in the case of the K9 modification was limited to mitotic cells on the luminal surface.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Text
0.9887	Specifically, both histone H3 trimethyl K9 and ProteinH4 monomethyl K20 were elevated in proliferating cells of the neural tube, which in the case of the K9 modification was limited to mitotic cells on the luminal surface.
0.9757	Specifically, both histone H3 trimethyl K9 and H4 monomethyl K20 were elevated in proliferating cells of the neural tube, which in the case of the EntityK9 modification was limited to mitotic cells on the luminal surface.
0.9641	Specifically, both histone H3 trimethyl K9 and H4 monomethyl K20 were elevated in proliferating cells of the neural tube, which in the case of the K9 Methylationmodification was limited to mitotic cells on the luminal surface.
0.9408	Specifically, both Proteinhistone H3 trimethyl K9 and H4 monomethyl K20 were elevated in proliferating cells of the neural tube, which in the case of the K9 modification was limited to mitotic cells on the luminal surface.
0.9344	Specifically, both histone H3 trimethyl EntityK9 and H4 monomethyl K20 were elevated in proliferating cells of the neural tube, which in the case of the K9 modification was limited to mitotic cells on the luminal surface.
0.8709	Specifically, both histone H3 trimethyl K9 and H4 monomethyl EntityK20 were elevated in proliferating cells of the neural tube, which in the case of the K9 modification was limited to mitotic cells on the luminal surface.

(1367)

In contrast, histone H4 trimethyl K20 was progressively lost from these medial regions and became enriched in differentiating neurons in the ventrolateral neural tube.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9584	In contrast, Proteinhistone H4 trimethyl K20 was progressively lost from these medial regions and became enriched in differentiating neurons in the ventrolateral neural tube.

(1368)

The inverse relationship of histone H4 K20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the trimethyl modification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Text
0.9813	The inverse relationship of histone H4 EntityK20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the trimethyl modification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.9358	The inverse relationship of Proteinhistone H4 K20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the trimethyl modification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.9302	The inverse relationship of histone H4 K20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the Methylationtrimethyl modification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.9676	The inverse relationship of histone H4 K20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the Methylationtrimethyl modification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.7513	The inverse relationship of histone H4 K20 Methylationmethyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the trimethyl modification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.7046	The inverse relationship of histone H4 K20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the trimethyl Methylationmodification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.

(1369)

Importantly, our results establish that histone lysine methylation occurs in a highly dynamic manner that is consistent with their function in an epigenetic program for cell division and differentiation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9796	Importantly, our results establish that histone lysine Methylationmethylation occurs in a highly dynamic manner that is consistent with their function in an epigenetic program for cell division and differentiation.
0.9606	Importantly, our results establish that histone Entitylysine methylation occurs in a highly dynamic manner that is consistent with their function in an epigenetic program for cell division and differentiation.
0.9308	Importantly, our results establish that Proteinhistone lysine methylation occurs in a highly dynamic manner that is consistent with their function in an epigenetic program for cell division and differentiation.

(1370)

Decreased expression of lysyl hydroxylase 2 ( LH2 ) in skin fibroblasts from three Ehlers - Danlos patients does not result from mutations in either the coding or proximal promoter region of the LH2 gene.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Decreased expression of lysyl hydroxylase 2 ( LH2 ) in skin fibroblasts from three Ehlers - Danlos patients does not result from mutations in either the coding or proximal promoter region of the ProteinLH2 gene.
0.9692	Decreased expression of lysyl hydroxylase 2 Protein( LH2 ) in skin fibroblasts from three Ehlers - Danlos patients does not result from mutations in either the coding or proximal promoter region of the LH2 gene.
0.8437	Decreased expression of Proteinlysyl hydroxylase 2 ( LH2 ) in skin fibroblasts from three Ehlers - Danlos patients does not result from mutations in either the coding or proximal promoter region of the LH2 gene.

(1371)

The Ehlers - Danlos syndromes ( EDS ) are a heterogeneous group of inherited connective tissue disorders characterized by tissue fragility, hyperelasticity of the skin and joint hypermobility.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1372)

This phenotype, accompanied by kyphoscoliosis and / or ocular fragility, is present in patients with the autosomal recessive type VI form of EDS.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1373)

These patients have significantly decreased levels of lysyl hydroxylase ( LH ) activity, due to mutations in the LH1 gene.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9967	These patients have significantly decreased levels of lysyl hydroxylase ( LH ) activity, due to mutations in the ProteinLH1 gene.

(1374)

LH hydroxylates specific lysine residues in the collagen molecule that are precursors for the formation of cross - links which provide collagen with its tensile strength.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.6730	LH Hydroxylationhydroxylates specific lysine residues in the collagen molecule that are precursors for the formation of cross - links which provide collagen with its tensile strength.

(1375)

No disorder has been directly linked to decreased expression of LH2 and LH3, two other isoforms of LH.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9988	No disorder has been directly linked to decreased expression of LH2 and ProteinLH3 , two other isoforms of LH.
0.9988	No disorder has been directly linked to decreased expression of ProteinLH2 and LH3, two other isoforms of LH.

(1376)

This study describes 3 patients with mixed phenotypes of EDS, who have significantly decreased mRNAs for LH2, but normal levels of LH1 and LH3 mRNAs, in their skin fibroblasts.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	This study describes 3 patients with mixed phenotypes of EDS, who have significantly decreased mRNAs for LH2, but normal levels of LH1 and ProteinLH3 mRNAs, in their skin fibroblasts.
0.9984	This study describes 3 patients with mixed phenotypes of EDS, who have significantly decreased mRNAs for ProteinLH2 , but normal levels of LH1 and LH3 mRNAs, in their skin fibroblasts.
0.9979	This study describes 3 patients with mixed phenotypes of EDS, who have significantly decreased mRNAs for LH2, but normal levels of ProteinLH1 and LH3 mRNAs, in their skin fibroblasts.

(1377)

In contrast to the effect of LH1 deficiency in EDS VI patients, the decreased expression of LH2 does not affect LH activity, bifunctional collagen cross - links ( measured after reduction as dihydroxylysinonorleucine ( DHLNL ) and hydroxylysinonorleucine ( HLNL ) ), or helical lysine hydroxylation in these cell lines.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9985	In contrast to the effect of ProteinLH1 deficiency in EDS VI patients, the decreased expression of LH2 does not affect LH activity, bifunctional collagen cross - links ( measured after reduction as dihydroxylysinonorleucine ( DHLNL ) and hydroxylysinonorleucine ( HLNL ) ), or helical lysine hydroxylation in these cell lines.
0.9984	In contrast to the effect of LH1 deficiency in EDS VI patients, the decreased expression of ProteinLH2 does not affect LH activity, bifunctional collagen cross - links ( measured after reduction as dihydroxylysinonorleucine ( DHLNL ) and hydroxylysinonorleucine ( HLNL ) ), or helical lysine hydroxylation in these cell lines.

(1378)

Sequence analysis of full length LH2 cDNAs and 1kb of the promoter region of LH2 does not show mutations that could explain the decreased expression of LH2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	Sequence analysis of full length LH2 cDNAs and 1kb of the promoter region of ProteinLH2 does not show mutations that could explain the decreased expression of LH2.
0.9995	Sequence analysis of full length LH2 cDNAs and 1kb of the promoter region of LH2 does not show mutations that could explain the decreased expression of ProteinLH2 .
0.9986	Sequence analysis of full length ProteinLH2 cDNAs and 1kb of the promoter region of LH2 does not show mutations that could explain the decreased expression of LH2.

(1379)

These results suggest that the deficiency of LH2 in these fibroblasts may be caused by changes in other factors required for the expression of LH2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	These results suggest that the deficiency of ProteinLH2 in these fibroblasts may be caused by changes in other factors required for the expression of LH2.
0.9995	These results suggest that the deficiency of LH2 in these fibroblasts may be caused by changes in other factors required for the expression of ProteinLH2 .

(1380)

Expression and purification of functionally active hyaluronan - binding domains from human cartilage link protein, aggrecan and versican : formation of ternary complexes with defined hyaluronan oligosaccharides.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	Expression and purification of functionally active hyaluronan - binding domains from human cartilage link protein, aggrecan and Proteinversican : formation of ternary complexes with defined hyaluronan oligosaccharides.
0.9995	Expression and purification of functionally active hyaluronan - binding domains from human cartilage link protein, Proteinaggrecan and versican : formation of ternary complexes with defined hyaluronan oligosaccharides.

(1381)

The chondroitin sulfate proteoglycan aggrecan forms link protein - stabilized complexes with hyaluronan ( HA ), via its N - terminal G1 - domain, that provide cartilage with its load bearing properties.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9984	The chondroitin sulfate proteoglycan Proteinaggrecan forms link protein - stabilized complexes with hyaluronan ( HA ), via its N - terminal G1 - domain, that provide cartilage with its load bearing properties.

(1382)

Similar aggregates ( potentially containing new members of the link protein family ), in which other chondroitin sulfate proteoglycans ( i. e. versican, brevican, and neurocan ) substitute for aggrecan, may contribute to the structural integrity of many other tissues including skin and brain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	Similar aggregates ( potentially containing new members of the link protein family ), in which other chondroitin sulfate proteoglycans ( i. e. versican, brevican, and neurocan ) substitute for Proteinaggrecan , may contribute to the structural integrity of many other tissues including skin and brain.
0.9986	Similar aggregates ( potentially containing new members of the link protein family ), in which other chondroitin sulfate proteoglycans ( i. e. versican, Proteinbrevican , and neurocan ) substitute for aggrecan, may contribute to the structural integrity of many other tissues including skin and brain.
0.9986	Similar aggregates ( potentially containing new members of the link protein family ), in which other chondroitin sulfate proteoglycans ( i. e. Proteinversican , brevican, and neurocan ) substitute for aggrecan, may contribute to the structural integrity of many other tissues including skin and brain.
0.9984	Similar aggregates ( potentially containing new members of the link protein family ), in which other chondroitin sulfate proteoglycans ( i. e. versican, brevican, and Proteinneurocan ) substitute for aggrecan, may contribute to the structural integrity of many other tissues including skin and brain.

(1383)

In this study, cartilage link protein ( cLP ) and the G1 - domains of aggrecan ( AG1 ) and versican ( VG1 ) were expressed in Drosophila S2 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9983	In this study, cartilage link protein ( cLP ) and the G1 - domains of Proteinaggrecan ( AG1 ) and versican ( VG1 ) were expressed in Drosophila S2 cells.
0.9975	In this study, cartilage link protein ( cLP ) and the G1 - domains of aggrecan ( AG1 ) and Proteinversican ( VG1 ) were expressed in Drosophila S2 cells.

(1384)

The recombinant human proteins were found to have properties similar to those described for the native molecules ( e. g. cLP was able to form oligomers, and HA decasaccharides were the minimum size that could compete effectively for their binding to polymeric HA ).

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Text
0.9383	The recombinant human proteins were found to have properties similar to those described for the native molecules ( e. g. cLP was able to form oligomers, and ProteinHA decasaccharides were the minimum size that could compete effectively for their binding to polymeric HA ).

(1385)

Gel filtration and protein cross - linking / matrix - assisted laser desorption ionization time - of - flight peptide fingerprinting showed that cLP and AG1 interact in the absence or presence of HA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1386)

Conversely, cLP and VG1 did not bind directly to each other in solution yet formed ternary complexes with HA24.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1387)

N - linked glycosylation of AG1 and VG1 was demonstrated to be unnecessary for either HA binding or the formation of ternary complexes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1388)

Surprisingly, the length of HA required to accommodate two G1 - domains was found to be significantly larger for aggrecan than versican, which may reflect differences in the conformation of HA stabilized on binding these proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9996	Surprisingly, the length of HA required to accommodate two G1 - domains was found to be significantly larger for aggrecan than Proteinversican , which may reflect differences in the conformation of HA stabilized on binding these proteins.
0.9995	Surprisingly, the length of HA required to accommodate two G1 - domains was found to be significantly larger for Proteinaggrecan than versican, which may reflect differences in the conformation of HA stabilized on binding these proteins.

(1389)

Protective mechanism of epigallocatechin - 3 - gallate against Helicobacter pylori - induced gastric epithelial cytotoxicity via the blockage of TLR - 4 signaling.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9968	Protective mechanism of epigallocatechin - 3 - gallate against Helicobacter pylori - induced gastric epithelial cytotoxicity via the blockage of ProteinTLR - 4 signaling.

(1390)

BACKGROUND : Helicobacter pylori infection leads to gastric mucosal damage by several mechanisms including the direct effect of virulence factors produced by H. pylori, propagation of inflammation, oxidative stress, DNA damage, and induction of apoptosis. ( - ) - Epigallocatechin - 3 - gallate ( EGCG ), one of the green tea catechins, is known to suppress H. pylori - induced gastritis through its antioxidative and antibacterial actions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1391)

In this study, we evaluated the protective mechanism of EGCG against H. pylori - induced cytotoxicity in gastric epithelial cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1392)

MATERIALS AND METHODS : MTT assays and dye exclusion assays were performed to analyze the effect of EGCG on the viability of gastric epithelial cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1393)

The degree of DNA damage was evaluated by Comet assay and apoptotic DNA fragmentation assay.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1394)

To investigate the effect of EGCG on H. pylori - induced toll - like receptor 4 ( TLR - 4 ) signaling, reverse transcription - polymerase chain reaction and Western blot analysis corresponding to glycosylated TLR - 4 were carried out.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9995	To investigate the effect of EGCG on H. pylori - induced toll - like receptor 4 ( TLR - 4 ) signaling, reverse transcription - polymerase chain reaction and Western blot analysis corresponding to glycosylated ProteinTLR - 4 were carried out.
0.9909	To investigate the effect of EGCG on H. pylori - induced toll - like receptor 4 ( TLR - 4 ) signaling, reverse transcription - polymerase chain reaction and Western blot analysis corresponding to Glycosylationglycosylated TLR - 4 were carried out.
0.9659	To investigate the effect of EGCG on H. pylori - induced toll - like receptor 4 Protein( TLR - 4 ) signaling, reverse transcription - polymerase chain reaction and Western blot analysis corresponding to glycosylated TLR - 4 were carried out.
0.7672	To investigate the effect of EGCG on H. pylori - induced Proteintoll - like receptor 4 ( TLR - 4 ) signaling, reverse transcription - polymerase chain reaction and Western blot analysis corresponding to glycosylated TLR - 4 were carried out.

(1395)

Lipoxygenase metabolites were measured with reverse - phase, high - performance liquid chromatography.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1396)

RESULTS : EGCG pretreatment effectively rescued gastric mucosal cells from the H. pylori - induced apoptotic cell death and DNA damage, and administration of this catechin enhanced gastric epithelial cell proliferation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1397)

Helicobacter pylori infection stimulated the glycosylation of TLR - 4, which initiates intracellular signaling in the infected host cell, but the pretreatment with EGCG completely blocked the TLR - 4 glycosylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9991	Helicobacter pylori infection stimulated the glycosylation of ProteinTLR - 4 , which initiates intracellular signaling in the infected host cell, but the pretreatment with EGCG completely blocked the TLR - 4 glycosylation.
0.9966	Helicobacter pylori infection stimulated the glycosylation of TLR - 4, which initiates intracellular signaling in the infected host cell, but the pretreatment with EGCG completely blocked the ProteinTLR - 4 glycosylation.
0.9873	Helicobacter pylori infection stimulated the Glycosylationglycosylation of TLR - 4, which initiates intracellular signaling in the infected host cell, but the pretreatment with EGCG completely blocked the TLR - 4 glycosylation.
0.9561	Helicobacter pylori infection stimulated the glycosylation of TLR - 4, which initiates intracellular signaling in the infected host cell, but the pretreatment with EGCG completely blocked the TLR - 4 Glycosylationglycosylation .

(1398)

The blockage of TLR - 4 activation by EGCG resulted in inactivation of extracellular signal response kinase 1 / 2 and of nuclear factor - kappaB, the downstream molecules of TLR - 4 signaling induced by H. pylori.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9998	The blockage of ProteinTLR - 4 activation by EGCG resulted in inactivation of extracellular signal response kinase 1 / 2 and of nuclear factor - kappaB, the downstream molecules of TLR - 4 signaling induced by H. pylori.
0.9996	The blockage of TLR - 4 activation by EGCG resulted in inactivation of extracellular signal response kinase 1 / 2 and of nuclear factor - kappaB, the downstream molecules of ProteinTLR - 4 signaling induced by H. pylori.
0.9632	The blockage of TLR - 4 activation by EGCG resulted in inactivation of extracellular signal response kinase Protein1 / 2 and of nuclear factor - kappaB, the downstream molecules of TLR - 4 signaling induced by H. pylori.
0.7716	The blockage of TLR - 4 activation by EGCG resulted in inactivation of Proteinextracellular signal response kinase 1 / 2 and of nuclear factor - kappaB, the downstream molecules of TLR - 4 signaling induced by H. pylori.

(1399)

This disturbance of H. pylori - induced host cell signaling by EGCG attenuated the synthesis of the proinflammatory mediator, hydroxyeicosatetraenoic acid.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1400)

CONCLUSIONS : EGCG pretreatment showed significant cytoprotective effects against H. pylori - induced gastric cytotoxicity via interference of the TLR - 4 signaling induced by H. pylori.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9979	CONCLUSIONS : EGCG pretreatment showed significant cytoprotective effects against H. pylori - induced gastric cytotoxicity via interference of the ProteinTLR - 4 signaling induced by H. pylori.

(1401)

Thus, our result implies that continuous intakes of green tea could prevent the deleterious consequences of H. pylori infection.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1402)

Bare - faced curassow lysozyme carrying amino acid substitutions at subsites E and F shows a change in activity against chitooligosaccharide caused by a local conformational change.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9966	Bare - faced curassow Proteinlysozyme carrying amino acid substitutions at subsites E and F shows a change in activity against chitooligosaccharide caused by a local conformational change.

(1403)

A new form of avian lysozyme, bare - faced curassow lysozyme ( BCL ), was purified and chemically sequenced.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9737	A new form of avian lysozyme, bare - faced curassow Proteinlysozyme ( BCL ), was purified and chemically sequenced.

(1404)

Of the 26 substitutions relative to chicken lysozyme, three, F34Y, T47S, and R114H, are of substrate - interacting residues in the E and F subsites, which would contribute to the acceptor binding for transglycosylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9986	Of the 26 substitutions relative to chicken Proteinlysozyme , three, F34Y, T47S, and R114H, are of substrate - interacting residues in the E and F subsites, which would contribute to the acceptor binding for transglycosylation.

(1405)

T47S is a novel substitution in this lysozyme class.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9817	T47S is a novel substitution in this Proteinlysozyme class.

(1406)

While other lysozymes also have substitutions at positions 114 and 34, they also contain numerous others, including ones in the other substrate binding sites, A - D.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9987	While other Proteinlysozymes also have substitutions at positions 114 and 34, they also contain numerous others, including ones in the other substrate binding sites, A - D.

(1407)

Furthermore, T47S lies on the left side, while F34Y and R114H are located on the right side of the E - F subsites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1408)

BCL therefore should allow comparison of the independent contributions of these sites to substrate binding and transglycosylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1409)

The activity toward the N - acetylglucosamine pentamer revealed that the substitutions at the E - F sites reduced the binding free energies at the E - F sites and the rate constant for transglycosylation without the conformation change of other substrate binding sites on the protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text

(1410)

MD simulation analysis of BCL suggested that the substituted amino acids changed the local conformation of this lysozyme at the E - F sites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9946	MD simulation analysis of BCL suggested that the substituted amino acids changed the local conformation of this Proteinlysozyme at the E - F sites.

(1411)

Histone H2B ubiquitylation is associated with elongating RNA polymerase II.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9727	Histone H2B Ubiquitinationubiquitylation is associated with elongating RNA polymerase II.
0.9419	ProteinHistone H2B ubiquitylation is associated with elongating RNA polymerase II.

(1412)

Rad6 - mediated ubiquitylation of histone H2B at lysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3.

Precision: 87.50

Recall: 100.00

F1: 93.33

Score	Text
0.9673	Rad6 - mediated ubiquitylation of histone H2B at lysine 123 has been linked to transcriptional activation and the regulation of lysine Methylationmethylation on histone H3.
0.9612	Rad6 - mediated Ubiquitinationubiquitylation of histone H2B at lysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3.
0.9571	Rad6 - mediated ubiquitylation of histone H2B at lysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on Proteinhistone H3 .
0.9294	Rad6 - mediated ubiquitylation of histone H2B at Entitylysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3.
0.9205	Rad6 - mediated ubiquitylation of histone H2B at lysine 123 has been linked to transcriptional activation and the regulation of Entitylysine methylation on histone H3.
0.9110	ProteinRad6 - mediated ubiquitylation of histone H2B at lysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3.
0.8977	Rad6 - mediated ubiquitylation of Proteinhistone H2B at lysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3.
0.5465	Rad6 - mediated ubiquitylation of histone H2B at Entitylysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3.

(1413)

However, how Rad6 and H2B ubiquitylation contribute to the transcription and histone methylation processes is poorly understood.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9952	However, how ProteinRad6 and H2B ubiquitylation contribute to the transcription and histone methylation processes is poorly understood.
0.9940	However, how Rad6 and ProteinH2B ubiquitylation contribute to the transcription and histone methylation processes is poorly understood.
0.9820	However, how Rad6 and H2B ubiquitylation contribute to the transcription and Proteinhistone methylation processes is poorly understood.
0.9460	However, how Rad6 and H2B Ubiquitinationubiquitylation contribute to the transcription and histone methylation processes is poorly understood.
0.8440	However, how Rad6 and H2B ubiquitylation contribute to the transcription and histone Methylationmethylation processes is poorly understood.

(1414)

Here, we show that the Paf1 transcription elongation complex and the E3 ligase for Rad6, Bre1, mediate an association of Rad6 with the hyperphosphorylated ( elongating ) form of RNA polymerase II ( Pol II ).

Precision: 80.00

Recall: 100.00

F1: 88.89

Score	Text
0.9996	Here, we show that the ProteinPaf1 transcription elongation complex and the E3 ligase for Rad6, Bre1, mediate an association of Rad6 with the hyperphosphorylated ( elongating ) form of RNA polymerase II ( Pol II ).
0.9991	Here, we show that the Paf1 transcription elongation complex and the E3 ligase for Rad6, ProteinBre1 , mediate an association of Rad6 with the hyperphosphorylated ( elongating ) form of RNA polymerase II ( Pol II ).
0.9991	Here, we show that the Paf1 transcription elongation complex and the E3 ligase for Rad6, Bre1, mediate an association of ProteinRad6 with the hyperphosphorylated ( elongating ) form of RNA polymerase II ( Pol II ).
0.9979	Here, we show that the Paf1 transcription elongation complex and the E3 ligase for ProteinRad6 , Bre1, mediate an association of Rad6 with the hyperphosphorylated ( elongating ) form of RNA polymerase II ( Pol II ).
0.6980	Here, we show that the Paf1 transcription elongation complex and the E3 ligase for Rad6, Bre1, mediate an association of Rad6 with the Phosphorylationhyperphosphorylated ( elongating ) form of RNA polymerase II ( Pol II ).

(1415)

This association appears to be necessary for the transcriptional activities of Rad6, as deletion of various Paf1 complex members or Bre1 abolishes H2B ubiquitylation ( ubH2B ) and reduces the recruitment of Rad6 to the promoters and transcribed regions of active genes.

Precision: 87.50

Recall: 100.00

F1: 93.33

Score	Text
0.9982	This association appears to be necessary for the transcriptional activities of Rad6, as deletion of various Paf1 complex members or ProteinBre1 abolishes H2B ubiquitylation ( ubH2B ) and reduces the recruitment of Rad6 to the promoters and transcribed regions of active genes.
0.9962	This association appears to be necessary for the transcriptional activities of Rad6, as deletion of various ProteinPaf1 complex members or Bre1 abolishes H2B ubiquitylation ( ubH2B ) and reduces the recruitment of Rad6 to the promoters and transcribed regions of active genes.
0.9946	This association appears to be necessary for the transcriptional activities of ProteinRad6 , as deletion of various Paf1 complex members or Bre1 abolishes H2B ubiquitylation ( ubH2B ) and reduces the recruitment of Rad6 to the promoters and transcribed regions of active genes.
0.9924	This association appears to be necessary for the transcriptional activities of Rad6, as deletion of various Paf1 complex members or Bre1 abolishes H2B ubiquitylation ( ubH2B ) and reduces the recruitment of ProteinRad6 to the promoters and transcribed regions of active genes.
0.9870	This association appears to be necessary for the transcriptional activities of Rad6, as deletion of various Paf1 complex members or Bre1 abolishes ProteinH2B ubiquitylation ( ubH2B ) and reduces the recruitment of Rad6 to the promoters and transcribed regions of active genes.
0.9690	This association appears to be necessary for the transcriptional activities of Rad6, as deletion of various Paf1 complex members or Bre1 abolishes H2B Ubiquitinationubiquitylation ( ubH2B ) and reduces the recruitment of Rad6 to the promoters and transcribed regions of active genes.
0.9536	This association appears to be necessary for the transcriptional activities of Rad6, as deletion of various Paf1 complex members or Bre1 abolishes H2B ubiquitylation Protein( ubH2B ) and reduces the recruitment of Rad6 to the promoters and transcribed regions of active genes.
0.5365	This association appears to be necessary for the transcriptional activities of Rad6, as deletion of various Paf1 complex members or Bre1 abolishes H2B Ubiquitinationubiquitylation ( ubH2B ) and reduces the recruitment of Rad6 to the promoters and transcribed regions of active genes.

(1416)

Using the inducible GAL1 gene as a model, we find that the recruitment of Rad6 upon activation occurs rapidly and transiently across the gene and coincides precisely with the appearance of Pol II.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9999	Using the inducible ProteinGAL1 gene as a model, we find that the recruitment of Rad6 upon activation occurs rapidly and transiently across the gene and coincides precisely with the appearance of Pol II.
0.9989	Using the inducible GAL1 gene as a model, we find that the recruitment of ProteinRad6 upon activation occurs rapidly and transiently across the gene and coincides precisely with the appearance of Pol II.

(1417)

Significantly, during GAL1 activation in an rtf1 deletion mutant, Rad6 accumulates at the promoter but is absent from the transcribed region.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9997	Significantly, during ProteinGAL1 activation in an rtf1 deletion mutant, Rad6 accumulates at the promoter but is absent from the transcribed region.
0.9996	Significantly, during GAL1 activation in an Proteinrtf1 deletion mutant, Rad6 accumulates at the promoter but is absent from the transcribed region.
0.9977	Significantly, during GAL1 activation in an rtf1 deletion mutant, ProteinRad6 accumulates at the promoter but is absent from the transcribed region.

(1418)

This fact suggests that Rad6 is recruited to promoters independently of the Paf1 complex but then requires this complex for entrance into the coding region of genes in a Pol II - associated manner.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9993	This fact suggests that Rad6 is recruited to promoters independently of the ProteinPaf1 complex but then requires this complex for entrance into the coding region of genes in a Pol II - associated manner.
0.9989	This fact suggests that ProteinRad6 is recruited to promoters independently of the Paf1 complex but then requires this complex for entrance into the coding region of genes in a Pol II - associated manner.

(1419)

In support of a role for Rad6 - dependent H2B ubiquitylation in transcription elongation, we find that ubH2B levels are dramatically reduced in strains bearing mutations of the Pol II C - terminal domain ( CTD ) and abolished by inactivation of Kin28, the serine 5 CTD kinase that promotes the transition from initiation to elongation.

Precision: 83.33

Recall: 100.00

F1: 90.91

Score	Text
0.9991	In support of a role for Rad6 - dependent H2B ubiquitylation in transcription elongation, we find that ProteinubH2B levels are dramatically reduced in strains bearing mutations of the Pol II C - terminal domain ( CTD ) and abolished by inactivation of Kin28, the serine 5 CTD kinase that promotes the transition from initiation to elongation.
0.9976	In support of a role for Rad6 - dependent H2B ubiquitylation in transcription elongation, we find that ubH2B levels are dramatically reduced in strains bearing mutations of the Pol II C - terminal domain ( CTD ) and abolished by inactivation of ProteinKin28 , the serine 5 CTD kinase that promotes the transition from initiation to elongation.
0.9699	In support of a role for Rad6 - dependent H2B Ubiquitinationubiquitylation in transcription elongation, we find that ubH2B levels are dramatically reduced in strains bearing mutations of the Pol II C - terminal domain ( CTD ) and abolished by inactivation of Kin28, the serine 5 CTD kinase that promotes the transition from initiation to elongation.
0.9572	In support of a role for Rad6 - dependent ProteinH2B ubiquitylation in transcription elongation, we find that ubH2B levels are dramatically reduced in strains bearing mutations of the Pol II C - terminal domain ( CTD ) and abolished by inactivation of Kin28, the serine 5 CTD kinase that promotes the transition from initiation to elongation.
0.9026	In support of a role for ProteinRad6 - dependent H2B ubiquitylation in transcription elongation, we find that ubH2B levels are dramatically reduced in strains bearing mutations of the Pol II C - terminal domain ( CTD ) and abolished by inactivation of Kin28, the serine 5 CTD kinase that promotes the transition from initiation to elongation.
0.8137	In support of a role for ProteinRad6 - dependent H2B ubiquitylation in transcription elongation, we find that ubH2B levels are dramatically reduced in strains bearing mutations of the Pol II C - terminal domain ( CTD ) and abolished by inactivation of Kin28, the serine 5 CTD kinase that promotes the transition from initiation to elongation.

(1420)

Furthermore, synthetic genetic array analysis reveals that the Rad6 complex interacts genetically with a number of known or suspected transcription elongation factors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9979	Furthermore, synthetic genetic array analysis reveals that the ProteinRad6 complex interacts genetically with a number of known or suspected transcription elongation factors.

(1421)

Finally, we show that Saccharomyces cerevisiae mutants bearing defects in the pathway to H2B ubiquitylation display transcription elongation defects as assayed by 6 - azauracil sensitivity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9909	Finally, we show that Saccharomyces cerevisiae mutants bearing defects in the pathway to ProteinH2B ubiquitylation display transcription elongation defects as assayed by 6 - azauracil sensitivity.
0.9725	Finally, we show that Saccharomyces cerevisiae mutants bearing defects in the pathway to H2B Ubiquitinationubiquitylation display transcription elongation defects as assayed by 6 - azauracil sensitivity.

(1422)

Collectively, our results indicate a role for Rad6 and H2B ubiquitylation during the elongation cycle of transcription and suggest a mechanism by which H3 methylation may be regulated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9955	Collectively, our results indicate a role for Rad6 and H2B ubiquitylation during the elongation cycle of transcription and suggest a mechanism by which ProteinH3 methylation may be regulated.
0.9910	Collectively, our results indicate a role for Rad6 and ProteinH2B ubiquitylation during the elongation cycle of transcription and suggest a mechanism by which H3 methylation may be regulated.
0.9833	Collectively, our results indicate a role for ProteinRad6 and H2B ubiquitylation during the elongation cycle of transcription and suggest a mechanism by which H3 methylation may be regulated.
0.9372	Collectively, our results indicate a role for Rad6 and H2B Ubiquitinationubiquitylation during the elongation cycle of transcription and suggest a mechanism by which H3 methylation may be regulated.
0.8328	Collectively, our results indicate a role for Rad6 and H2B ubiquitylation during the elongation cycle of transcription and suggest a mechanism by which H3 Methylationmethylation may be regulated.

(1423)

Glycosylation - related gene expression in prion diseases : PrPSc accumulation in scrapie infected GT1 cells depends on beta - 1, 4 - linked GalNAc - 4 - SO4 hyposulfation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Text
0.9972	Glycosylation - related gene expression in prion diseases : ProteinPrPSc accumulation in scrapie infected GT1 cells depends on beta - 1, 4 - linked GalNAc - 4 - SO4 hyposulfation.
0.9940	Glycosylation - related gene expression in Proteinprion diseases : PrPSc accumulation in scrapie infected GT1 cells depends on beta - 1, 4 - linked GalNAc - 4 - SO4 hyposulfation.

Entity Extraction Examples (1424)